Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria.
Identifieur interne : 001B59 ( PubMed/Checkpoint ); précédent : 001B58; suivant : 001B60Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria.
Auteurs : Eva C. Boritsch [France] ; Varun Khanna [France] ; Alexandre Pawlik [France] ; Nadine Honoré [France] ; Victor H. Navas [France] ; Laurence Ma [France] ; Christiane Bouchier [France] ; Torsten Seemann [Australie] ; Philip Supply [France] ; Timothy P. Stinear [Australie] ; Roland Brosch [France]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2016.
Abstract
Horizontal gene transfer (HGT) is a major driving force of bacterial diversification and evolution. For tuberculosis-causing mycobacteria, the impact of HGT in the emergence and distribution of dominant lineages remains a matter of debate. Here, by using fluorescence-assisted mating assays and whole genome sequencing, we present unique experimental evidence of chromosomal DNA transfer between tubercle bacilli of the early-branching Mycobacterium canettii clade. We found that the obtained recombinants had received multiple donor-derived DNA fragments in the size range of 100 bp to 118 kbp, fragments large enough to contain whole operons. Although the transfer frequency between M. canettii strains was low and no transfer could be observed among classical Mycobacterium tuberculosis complex (MTBC) strains, our study provides the proof of concept for genetic exchange in tubercle bacilli. This outstanding, now experimentally validated phenomenon presumably played a key role in the early evolution of the MTBC toward pathogenicity. Moreover, our findings also provide important information for the risk evaluation of potential transfer of drug resistance and fitness mutations among clinically relevant mycobacterial strains.
DOI: 10.1073/pnas.1604921113
PubMed: 27528665
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Boritsch</name><affiliation wicri:level="3"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Khanna, Varun" sort="Khanna, Varun" uniqKey="Khanna V" first="Varun" last="Khanna">Varun Khanna</name><affiliation wicri:level="3"><nlm:affiliation>IP, Hub Bioinformatique et Biostatistique, C3BI, Unité de Services et de Recherche, USR 3756, IP CNRS, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, Hub Bioinformatique et Biostatistique, C3BI, Unité de Services et de Recherche, USR 3756, IP CNRS, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Pawlik, Alexandre" sort="Pawlik, Alexandre" uniqKey="Pawlik A" first="Alexandre" last="Pawlik">Alexandre Pawlik</name><affiliation wicri:level="3"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Honore, Nadine" sort="Honore, Nadine" uniqKey="Honore N" first="Nadine" last="Honoré">Nadine Honoré</name><affiliation wicri:level="3"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Navas, Victor H" sort="Navas, Victor H" uniqKey="Navas V" first="Victor H" last="Navas">Victor H. Navas</name><affiliation wicri:level="3"><nlm:affiliation>IP, Lymphocyte Cell Biology Unit, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, Lymphocyte Cell Biology Unit, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Ma, Laurence" sort="Ma, Laurence" uniqKey="Ma L" first="Laurence" last="Ma">Laurence Ma</name><affiliation wicri:level="3"><nlm:affiliation>IP, PF1-Plate-Forme Génomique, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, PF1-Plate-Forme Génomique, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Bouchier, Christiane" sort="Bouchier, Christiane" uniqKey="Bouchier C" first="Christiane" last="Bouchier">Christiane Bouchier</name><affiliation wicri:level="3"><nlm:affiliation>IP, PF1-Plate-Forme Génomique, 75015 Paris, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IP, PF1-Plate-Forme Génomique, 75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Seemann, Torsten" sort="Seemann, Torsten" uniqKey="Seemann T" first="Torsten" last="Seemann">Torsten Seemann</name><affiliation wicri:level="4"><nlm:affiliation>Victorian Life Sciences Computation Initiative, University of Melbourne, Carlton, VIC 3053, Australia;</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Victorian Life Sciences Computation Initiative, University of Melbourne, Carlton, VIC 3053</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Supply, Philip" sort="Supply, Philip" uniqKey="Supply P" first="Philip" last="Supply">Philip Supply</name><affiliation wicri:level="1"><nlm:affiliation>INSERM U1019, CNRS UMR 8204, Center for Infection and Immunity, Institut Pasteur de Lille, Université de Lille, 59000 Lille, France;</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INSERM U1019, CNRS UMR 8204, Center for Infection and Immunity, Institut Pasteur de Lille, Université de Lille, 59000 Lille</wicri:regionArea><wicri:noRegion>59000 Lille</wicri:noRegion><wicri:noRegion>59000 Lille</wicri:noRegion></affiliation></author><author><name sortKey="Stinear, Timothy P" sort="Stinear, Timothy P" uniqKey="Stinear T" first="Timothy P" last="Stinear">Timothy P. Stinear</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC 3000, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC 3000</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Brosch, Roland" sort="Brosch, Roland" uniqKey="Brosch R" first="Roland" last="Brosch">Roland Brosch</name><affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France; roland.brosch@pasteur.fr.</nlm:affiliation><country wicri:rule="url">France</country><wicri:regionArea>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris</wicri:regionArea><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Horizontal gene transfer (HGT) is a major driving force of bacterial diversification and evolution. For tuberculosis-causing mycobacteria, the impact of HGT in the emergence and distribution of dominant lineages remains a matter of debate. Here, by using fluorescence-assisted mating assays and whole genome sequencing, we present unique experimental evidence of chromosomal DNA transfer between tubercle bacilli of the early-branching Mycobacterium canettii clade. We found that the obtained recombinants had received multiple donor-derived DNA fragments in the size range of 100 bp to 118 kbp, fragments large enough to contain whole operons. Although the transfer frequency between M. canettii strains was low and no transfer could be observed among classical Mycobacterium tuberculosis complex (MTBC) strains, our study provides the proof of concept for genetic exchange in tubercle bacilli. This outstanding, now experimentally validated phenomenon presumably played a key role in the early evolution of the MTBC toward pathogenicity. Moreover, our findings also provide important information for the risk evaluation of potential transfer of drug resistance and fitness mutations among clinically relevant mycobacterial strains.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">27528665</PMID><DateCreated><Year>2016</Year><Month>08</Month><Day>31</Day></DateCreated><DateRevised><Year>2017</Year><Month>02</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN><JournalIssue CitedMedium="Internet"><Volume>113</Volume><Issue>35</Issue><PubDate><Year>2016</Year><Month>Aug</Month><Day>30</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc. Natl. Acad. Sci. U.S.A.</ISOAbbreviation></Journal><ArticleTitle>Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria.</ArticleTitle><Pagination><MedlinePgn>9876-81</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.1604921113</ELocationID><Abstract><AbstractText>Horizontal gene transfer (HGT) is a major driving force of bacterial diversification and evolution. For tuberculosis-causing mycobacteria, the impact of HGT in the emergence and distribution of dominant lineages remains a matter of debate. Here, by using fluorescence-assisted mating assays and whole genome sequencing, we present unique experimental evidence of chromosomal DNA transfer between tubercle bacilli of the early-branching Mycobacterium canettii clade. We found that the obtained recombinants had received multiple donor-derived DNA fragments in the size range of 100 bp to 118 kbp, fragments large enough to contain whole operons. Although the transfer frequency between M. canettii strains was low and no transfer could be observed among classical Mycobacterium tuberculosis complex (MTBC) strains, our study provides the proof of concept for genetic exchange in tubercle bacilli. This outstanding, now experimentally validated phenomenon presumably played a key role in the early evolution of the MTBC toward pathogenicity. Moreover, our findings also provide important information for the risk evaluation of potential transfer of drug resistance and fitness mutations among clinically relevant mycobacterial strains.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Boritsch</LastName><ForeName>Eva C</ForeName><Initials>EC</Initials><AffiliationInfo><Affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khanna</LastName><ForeName>Varun</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>IP, Hub Bioinformatique et Biostatistique, C3BI, Unité de Services et de Recherche, USR 3756, IP CNRS, 75015 Paris, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pawlik</LastName><ForeName>Alexandre</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Honoré</LastName><ForeName>Nadine</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Navas</LastName><ForeName>Victor H</ForeName><Initials>VH</Initials><AffiliationInfo><Affiliation>IP, Lymphocyte Cell Biology Unit, 75015 Paris, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Laurence</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>IP, PF1-Plate-Forme Génomique, 75015 Paris, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bouchier</LastName><ForeName>Christiane</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>IP, PF1-Plate-Forme Génomique, 75015 Paris, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Seemann</LastName><ForeName>Torsten</ForeName><Initials>T</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6046-610X</Identifier><AffiliationInfo><Affiliation>Victorian Life Sciences Computation Initiative, University of Melbourne, Carlton, VIC 3053, Australia;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Supply</LastName><ForeName>Philip</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>INSERM U1019, CNRS UMR 8204, Center for Infection and Immunity, Institut Pasteur de Lille, Université de Lille, 59000 Lille, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stinear</LastName><ForeName>Timothy P</ForeName><Initials>TP</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-0150-123X</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC 3000, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brosch</LastName><ForeName>Roland</ForeName><Initials>R</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-2587-3863</Identifier><AffiliationInfo><Affiliation>Institut Pasteur (IP), Unit for Integrated Mycobacterial Pathogenomics, 75015 Paris, France; roland.brosch@pasteur.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>08</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Mol Microbiol. 1998 Sep;29(6):1331-9</RefSource><PMID Version="1">9781872</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Lancet. 1994 Jul 30;344(8918):293-8</RefSource><PMID Version="1">7914261</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Med Trop (Mars). 2002;62(1):70-2</RefSource><PMID Version="1">12038183</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Genome Res. 2012 Apr;22(4):721-34</RefSource><PMID Version="1">22377718</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13264-71</RefSource><PMID Version="1">25197070</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Genet. 2010 Jun;42(6):498-503</RefSource><PMID Version="1">20495566</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Microbiol. 2004 Aug;53(4):1233-41</RefSource><PMID Version="1">15306024</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Microbiol. 2008 Aug;69(4):794-808</RefSource><PMID Version="1">18554329</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Genome Biol Evol. 2016 Jan 08;8(2):387-402</RefSource><PMID Version="1">26748339</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Microbiol. 2003 Jan;47(2):529-38</RefSource><PMID Version="1">12519202</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12598-603</RefSource><PMID Version="1">15314236</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12420-5</RefSource><PMID Version="1">14557547</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Genome Biol Evol. 2014 Aug 30;6(9):2489-500</RefSource><PMID Version="1">25173757</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Tuber Lung Dis. 1999;79(3):171-80</RefSource><PMID Version="1">10656115</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10955-60</RefSource><PMID Version="1">9380741</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Lancet. 1993 Mar 13;341(8846):647-50</RefSource><PMID Version="1">8095569</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Microbiol. 2014 Sep;93(5):835-52</RefSource><PMID Version="1">25039682</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Microbiol. 1998 Sep;29(5):1203-14</RefSource><PMID Version="1">9767588</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS Pathog. 2005 Sep;1(1):e5</RefSource><PMID Version="1">16201017</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2011;6(6):e20499</RefSource><PMID Version="1">21673985</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Lancet Infect Dis. 2007 May;7(5):328-37</RefSource><PMID Version="1">17448936</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Bacteriol. 2006 Dec;188(23):8169-77</RefSource><PMID Version="1">16997954</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Microbiol. 1998 May;28(3):571-82</RefSource><PMID Version="1">9632259</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Emerg Infect Dis. 2004 Sep;10(9):1568-77</RefSource><PMID Version="1">15498158</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Genet. 2013 Feb;45(2):172-9</RefSource><PMID Version="1">23291586</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS Biol. 2013 Jul;11(7):e1001602</RefSource><PMID Version="1">23874149</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Clin Microbiol. 2004 Jul;42(7):3248-55</RefSource><PMID Version="1">15243089</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9869-74</RefSource><PMID Version="1">9275218</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Biol Chem. 2005 Oct 7;280(40):33953-9</RefSource><PMID Version="1">16048998</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Microbiol. 2016 Jan 27;1:15019</RefSource><PMID Version="1">27571976</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3684-9</RefSource><PMID Version="1">11891304</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7877-82</RefSource><PMID Version="1">12788972</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cold Spring Harb Symp Quant Biol. 1956;21:141-62</RefSource><PMID Version="1">13433587</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Rev Microbiol. 2005 Sep;3(9):711-21</RefSource><PMID Version="1">16138099</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Clin Microbiol Infect. 2011 Jul;17(7):1013-9</RefSource><PMID Version="1">20831613</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Infect Dis. 2002 Jul 1;186(1):74-80</RefSource><PMID Version="1">12089664</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Front Cell Infect Microbiol. 2012 Aug 21;2:110</RefSource><PMID Version="1">22919697</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10961-6</RefSource><PMID Version="1">9380742</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15961-6</RefSource><PMID Version="1">18832470</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Int J Syst Bacteriol. 1997 Oct;47(4):1236-45</RefSource><PMID Version="1">9336935</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Future Microbiol. 2013 Nov;8(11):1405-18</RefSource><PMID Version="1">24199800</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Emerg Infect Dis. 2014 Jan;20(1):21-8</RefSource><PMID Version="1">24520560</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Bacteriol. 2011 Mar;193(5):1114-21</RefSource><PMID Version="1">21169481</PMID></CommentsCorrections></CommentsCorrectionsList><OtherID Source="NLM">PMC5024641 [Available on 02/28/17]</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">DNA transfer</Keyword><Keyword MajorTopicYN="N">Mycobacterium canettii</Keyword><Keyword MajorTopicYN="N">evolution</Keyword><Keyword MajorTopicYN="N">recombination</Keyword><Keyword MajorTopicYN="N">tuberculosis</Keyword></KeywordList><CoiStatement>The authors declare no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pmc-release"><Year>2017</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>8</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>8</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>8</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27528665</ArticleId><ArticleId IdType="pii">1604921113</ArticleId><ArticleId IdType="doi">10.1073/pnas.1604921113</ArticleId><ArticleId IdType="pmc">PMC5024641</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Victoria (État)</li><li>Île-de-France</li></region><settlement><li>Melbourne</li><li>Paris</li></settlement><orgName><li>Université de Melbourne</li></orgName></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Boritsch, Eva C" sort="Boritsch, Eva C" uniqKey="Boritsch E" first="Eva C" last="Boritsch">Eva C. Boritsch</name></region><name sortKey="Bouchier, Christiane" sort="Bouchier, Christiane" uniqKey="Bouchier C" first="Christiane" last="Bouchier">Christiane Bouchier</name><name sortKey="Brosch, Roland" sort="Brosch, Roland" uniqKey="Brosch R" first="Roland" last="Brosch">Roland Brosch</name><name sortKey="Honore, Nadine" sort="Honore, Nadine" uniqKey="Honore N" first="Nadine" last="Honoré">Nadine Honoré</name><name sortKey="Khanna, Varun" sort="Khanna, Varun" uniqKey="Khanna V" first="Varun" last="Khanna">Varun Khanna</name><name sortKey="Ma, Laurence" sort="Ma, Laurence" uniqKey="Ma L" first="Laurence" last="Ma">Laurence Ma</name><name sortKey="Navas, Victor H" sort="Navas, Victor H" uniqKey="Navas V" first="Victor H" last="Navas">Victor H. Navas</name><name sortKey="Pawlik, Alexandre" sort="Pawlik, Alexandre" uniqKey="Pawlik A" first="Alexandre" last="Pawlik">Alexandre Pawlik</name><name sortKey="Supply, Philip" sort="Supply, Philip" uniqKey="Supply P" first="Philip" last="Supply">Philip Supply</name></country><country name="Australie"><region name="Victoria (État)"><name sortKey="Seemann, Torsten" sort="Seemann, Torsten" uniqKey="Seemann T" first="Torsten" last="Seemann">Torsten Seemann</name></region><name sortKey="Stinear, Timothy P" sort="Stinear, Timothy P" uniqKey="Stinear T" first="Timothy P" last="Stinear">Timothy P. Stinear</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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