Xer Recombinase and Genome Integrity in Helicobacter pylori, a Pathogen without Topoisomerase IV
Identifieur interne : 002859 ( Pmc/Curation ); précédent : 002858; suivant : 002860Xer Recombinase and Genome Integrity in Helicobacter pylori, a Pathogen without Topoisomerase IV
Auteurs : Aleksandra W. Debowski [États-Unis] ; Christophe Carnoy [France] ; Phebe Verbrugghe [États-Unis] ; Hans-Olof Nilsson [États-Unis] ; Jonathan C. Gauntlett [États-Unis] ; Alma Fulurija [États-Unis] ; Tania Camilleri [États-Unis] ; Douglas E. Berg [États-Unis] ; Barry J. Marshall [États-Unis] ; Mohammed Benghezal [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Abstract
In the model organism
Url:
DOI: 10.1371/journal.pone.0033310
PubMed: 22511919
PubMed Central: 3325230
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002A09
Links to Exploration step
PMC:3325230Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Xer Recombinase and Genome Integrity in <italic>Helicobacter pylori</italic>
, a Pathogen without Topoisomerase IV</title>
<author><name sortKey="Debowski, Aleksandra W" sort="Debowski, Aleksandra W" uniqKey="Debowski A" first="Aleksandra W." last="Debowski">Aleksandra W. Debowski</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Carnoy, Christophe" sort="Carnoy, Christophe" uniqKey="Carnoy C" first="Christophe" last="Carnoy">Christophe Carnoy</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>United States of America Center for Infection and Immunity of Lille, INSERM U 1019, CNRS UMR 8204, Univ Lille Nord de France, Institut Pasteur de Lille, Lille, France</addr-line>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>United States of America Center for Infection and Immunity of Lille, INSERM U 1019, CNRS UMR 8204, Univ Lille Nord de France, Institut Pasteur de Lille, Lille</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Verbrugghe, Phebe" sort="Verbrugghe, Phebe" uniqKey="Verbrugghe P" first="Phebe" last="Verbrugghe">Phebe Verbrugghe</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Nilsson, Hans Olof" sort="Nilsson, Hans Olof" uniqKey="Nilsson H" first="Hans-Olof" last="Nilsson">Hans-Olof Nilsson</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Gauntlett, Jonathan C" sort="Gauntlett, Jonathan C" uniqKey="Gauntlett J" first="Jonathan C." last="Gauntlett">Jonathan C. Gauntlett</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Fulurija, Alma" sort="Fulurija, Alma" uniqKey="Fulurija A" first="Alma" last="Fulurija">Alma Fulurija</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Camilleri, Tania" sort="Camilleri, Tania" uniqKey="Camilleri T" first="Tania" last="Camilleri">Tania Camilleri</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Berg, Douglas E" sort="Berg, Douglas E" uniqKey="Berg D" first="Douglas E." last="Berg">Douglas E. Berg</name>
<affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Marshall, Barry J" sort="Marshall, Barry J" uniqKey="Marshall B" first="Barry J." last="Marshall">Barry J. Marshall</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Benghezal, Mohammed" sort="Benghezal, Mohammed" uniqKey="Benghezal M" first="Mohammed" last="Benghezal">Mohammed Benghezal</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">22511919</idno>
<idno type="pmc">3325230</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3325230</idno>
<idno type="RBID">PMC:3325230</idno>
<idno type="doi">10.1371/journal.pone.0033310</idno>
<date when="2012">2012</date>
<idno type="wicri:Area/Pmc/Corpus">002A09</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002A09</idno>
<idno type="wicri:Area/Pmc/Curation">002859</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002859</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Xer Recombinase and Genome Integrity in <italic>Helicobacter pylori</italic>
, a Pathogen without Topoisomerase IV</title>
<author><name sortKey="Debowski, Aleksandra W" sort="Debowski, Aleksandra W" uniqKey="Debowski A" first="Aleksandra W." last="Debowski">Aleksandra W. Debowski</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Carnoy, Christophe" sort="Carnoy, Christophe" uniqKey="Carnoy C" first="Christophe" last="Carnoy">Christophe Carnoy</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>United States of America Center for Infection and Immunity of Lille, INSERM U 1019, CNRS UMR 8204, Univ Lille Nord de France, Institut Pasteur de Lille, Lille, France</addr-line>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>United States of America Center for Infection and Immunity of Lille, INSERM U 1019, CNRS UMR 8204, Univ Lille Nord de France, Institut Pasteur de Lille, Lille</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Verbrugghe, Phebe" sort="Verbrugghe, Phebe" uniqKey="Verbrugghe P" first="Phebe" last="Verbrugghe">Phebe Verbrugghe</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Nilsson, Hans Olof" sort="Nilsson, Hans Olof" uniqKey="Nilsson H" first="Hans-Olof" last="Nilsson">Hans-Olof Nilsson</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Gauntlett, Jonathan C" sort="Gauntlett, Jonathan C" uniqKey="Gauntlett J" first="Jonathan C." last="Gauntlett">Jonathan C. Gauntlett</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Fulurija, Alma" sort="Fulurija, Alma" uniqKey="Fulurija A" first="Alma" last="Fulurija">Alma Fulurija</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Camilleri, Tania" sort="Camilleri, Tania" uniqKey="Camilleri T" first="Tania" last="Camilleri">Tania Camilleri</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Berg, Douglas E" sort="Berg, Douglas E" uniqKey="Berg D" first="Douglas E." last="Berg">Douglas E. Berg</name>
<affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, United States of America</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Marshall, Barry J" sort="Marshall, Barry J" uniqKey="Marshall B" first="Barry J." last="Marshall">Barry J. Marshall</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Benghezal, Mohammed" sort="Benghezal, Mohammed" uniqKey="Benghezal M" first="Mohammed" last="Benghezal">Mohammed Benghezal</name>
<affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands</wicri:cityArea>
</affiliation>
</author>
</analytic>
<series><title level="j">PLoS ONE</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>In the model organism <italic>E. coli</italic>
, recombination mediated by the related XerC and XerD recombinases complexed with the FtsK translocase at specialized <italic>dif</italic>
sites, resolves dimeric chromosomes into free monomers to allow efficient chromosome segregation at cell division. Computational genome analysis of <italic>Helicobacter pylori</italic>
, a slow growing gastric pathogen, identified just one chromosomal <italic>xer</italic>
gene (<italic>xerH</italic>
) and its cognate <italic>dif</italic>
site (<italic>difH</italic>
). Here we show that recombination between directly repeated <italic>difH</italic>
sites requires XerH, FtsK but not XerT, the TnPZ transposon associated recombinase. <italic>xerH</italic>
inactivation was not lethal, but resulted in increased DNA per cell, suggesting defective chromosome segregation. The <italic>xerH</italic>
mutant also failed to colonize mice, and was more susceptible to UV and ciprofloxacin, which induce DNA breakage, and thereby recombination and chromosome dimer formation. <italic>xerH</italic>
inactivation and overexpression each led to a DNA segregation defect, suggesting a role for Xer recombination in regulation of replication. In addition to chromosome dimer resolution and based on the absence of genes for topoisomerase IV (<italic>parC</italic>
, <italic>parE</italic>
) in <italic>H. pylori</italic>
, we speculate that XerH may contribute to chromosome decatenation, although possible involvement of <italic>H. pylori</italic>
's DNA gyrase and topoisomerase III homologue are also considered. Further analyses of this system should contribute to general understanding of and possibly therapy development for <italic>H. pylori</italic>
, which causes peptic ulcers and gastric cancer; for the closely related, diarrheagenic Campylobacter species; and for unrelated slow growing pathogens that lack topoisomerase IV, such as <italic>Mycobacterium tuberculosis</italic>
.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Blakely, G" uniqKey="Blakely G">G Blakely</name>
</author>
<author><name sortKey="May, G" uniqKey="May G">G May</name>
</author>
<author><name sortKey="Mcculloch, R" uniqKey="Mcculloch R">R McCulloch</name>
</author>
<author><name sortKey="Arciszewska, Lk" uniqKey="Arciszewska L">LK Arciszewska</name>
</author>
<author><name sortKey="Burke, M" uniqKey="Burke M">M Burke</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Aussel, L" uniqKey="Aussel L">L Aussel</name>
</author>
<author><name sortKey="Barre, Fx" uniqKey="Barre F">FX Barre</name>
</author>
<author><name sortKey="Aroyo, M" uniqKey="Aroyo M">M Aroyo</name>
</author>
<author><name sortKey="Stasiak, A" uniqKey="Stasiak A">A Stasiak</name>
</author>
<author><name sortKey="Stasiak, Az" uniqKey="Stasiak A">AZ Stasiak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Leslie, Nr" uniqKey="Leslie N">NR Leslie</name>
</author>
<author><name sortKey="Sherratt, Dj" uniqKey="Sherratt D">DJ Sherratt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huisman, O" uniqKey="Huisman O">O Huisman</name>
</author>
<author><name sortKey="D Ari, R" uniqKey="D Ari R">R D'Ari</name>
</author>
<author><name sortKey="Gottesman, S" uniqKey="Gottesman S">S Gottesman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crozat, E" uniqKey="Crozat E">E Crozat</name>
</author>
<author><name sortKey="Grainge, I" uniqKey="Grainge I">I Grainge</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hendrickson, H" uniqKey="Hendrickson H">H Hendrickson</name>
</author>
<author><name sortKey="Lawrence, Jg" uniqKey="Lawrence J">JG Lawrence</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grainge, I" uniqKey="Grainge I">I Grainge</name>
</author>
<author><name sortKey="Bregu, M" uniqKey="Bregu M">M Bregu</name>
</author>
<author><name sortKey="Vazquez, M" uniqKey="Vazquez M">M Vazquez</name>
</author>
<author><name sortKey="Sivanathan, V" uniqKey="Sivanathan V">V Sivanathan</name>
</author>
<author><name sortKey="Ip, Sc" uniqKey="Ip S">SC Ip</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zechiedrich, El" uniqKey="Zechiedrich E">EL Zechiedrich</name>
</author>
<author><name sortKey="Cozzarelli, Nr" uniqKey="Cozzarelli N">NR Cozzarelli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kato, J" uniqKey="Kato J">J Kato</name>
</author>
<author><name sortKey="Nishimura, Y" uniqKey="Nishimura Y">Y Nishimura</name>
</author>
<author><name sortKey="Imamura, R" uniqKey="Imamura R">R Imamura</name>
</author>
<author><name sortKey="Niki, H" uniqKey="Niki H">H Niki</name>
</author>
<author><name sortKey="Hiraga, S" uniqKey="Hiraga S">S Hiraga</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ip, Sc" uniqKey="Ip S">SC Ip</name>
</author>
<author><name sortKey="Bregu, M" uniqKey="Bregu M">M Bregu</name>
</author>
<author><name sortKey="Barre, Fx" uniqKey="Barre F">FX Barre</name>
</author>
<author><name sortKey="Sherratt, Dj" uniqKey="Sherratt D">DJ Sherratt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Espeli, O" uniqKey="Espeli O">O Espeli</name>
</author>
<author><name sortKey="Lee, C" uniqKey="Lee C">C Lee</name>
</author>
<author><name sortKey="Marians, Kj" uniqKey="Marians K">KJ Marians</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Carnoy, C" uniqKey="Carnoy C">C Carnoy</name>
</author>
<author><name sortKey="Floquet, S" uniqKey="Floquet S">S Floquet</name>
</author>
<author><name sortKey="Marceau, M" uniqKey="Marceau M">M Marceau</name>
</author>
<author><name sortKey="Sebbane, F" uniqKey="Sebbane F">F Sebbane</name>
</author>
<author><name sortKey="Haentjens Herwegh, S" uniqKey="Haentjens Herwegh S">S Haentjens-Herwegh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kono, N" uniqKey="Kono N">N Kono</name>
</author>
<author><name sortKey="Arakawa, K" uniqKey="Arakawa K">K Arakawa</name>
</author>
<author><name sortKey="Tomita, M" uniqKey="Tomita M">M Tomita</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Carnoy, C" uniqKey="Carnoy C">C Carnoy</name>
</author>
<author><name sortKey="Roten, Ca" uniqKey="Roten C">CA Roten</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Le Bourgeois, P" uniqKey="Le Bourgeois P">P Le Bourgeois</name>
</author>
<author><name sortKey="Bugarel, M" uniqKey="Bugarel M">M Bugarel</name>
</author>
<author><name sortKey="Campo, N" uniqKey="Campo N">N Campo</name>
</author>
<author><name sortKey="Daveran Mingot, Ml" uniqKey="Daveran Mingot M">ML Daveran-Mingot</name>
</author>
<author><name sortKey="Labonte, J" uniqKey="Labonte J">J Labonte</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sciochetti, Sa" uniqKey="Sciochetti S">SA Sciochetti</name>
</author>
<author><name sortKey="Piggot, Pj" uniqKey="Piggot P">PJ Piggot</name>
</author>
<author><name sortKey="Blakely, Gw" uniqKey="Blakely G">GW Blakely</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cortez, D" uniqKey="Cortez D">D Cortez</name>
</author>
<author><name sortKey="Quevillon Cheruel, S" uniqKey="Quevillon Cheruel S">S Quevillon-Cheruel</name>
</author>
<author><name sortKey="Gribaldo, S" uniqKey="Gribaldo S">S Gribaldo</name>
</author>
<author><name sortKey="Desnoues, N" uniqKey="Desnoues N">N Desnoues</name>
</author>
<author><name sortKey="Sezonov, G" uniqKey="Sezonov G">G Sezonov</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Duggin, Ig" uniqKey="Duggin I">IG Duggin</name>
</author>
<author><name sortKey="Dubarry, N" uniqKey="Dubarry N">N Dubarry</name>
</author>
<author><name sortKey="Bell, Sd" uniqKey="Bell S">SD Bell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ambur, Oh" uniqKey="Ambur O">OH Ambur</name>
</author>
<author><name sortKey="Davidsen, T" uniqKey="Davidsen T">T Davidsen</name>
</author>
<author><name sortKey="Frye, Sa" uniqKey="Frye S">SA Frye</name>
</author>
<author><name sortKey="Balasingham, Sv" uniqKey="Balasingham S">SV Balasingham</name>
</author>
<author><name sortKey="Lagesen, K" uniqKey="Lagesen K">K Lagesen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fischer, W" uniqKey="Fischer W">W Fischer</name>
</author>
<author><name sortKey="Windhager, L" uniqKey="Windhager L">L Windhager</name>
</author>
<author><name sortKey="Rohrer, S" uniqKey="Rohrer S">S Rohrer</name>
</author>
<author><name sortKey="Zeiller, M" uniqKey="Zeiller M">M Zeiller</name>
</author>
<author><name sortKey="Karnholz, A" uniqKey="Karnholz A">A Karnholz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kersulyte, D" uniqKey="Kersulyte D">D Kersulyte</name>
</author>
<author><name sortKey="Lee, W" uniqKey="Lee W">W Lee</name>
</author>
<author><name sortKey="Subramaniam, D" uniqKey="Subramaniam D">D Subramaniam</name>
</author>
<author><name sortKey="Anant, S" uniqKey="Anant S">S Anant</name>
</author>
<author><name sortKey="Herrera, P" uniqKey="Herrera P">P Herrera</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dailidiene, D" uniqKey="Dailidiene D">D Dailidiene</name>
</author>
<author><name sortKey="Dailide, G" uniqKey="Dailide G">G Dailide</name>
</author>
<author><name sortKey="Kersulyte, D" uniqKey="Kersulyte D">D Kersulyte</name>
</author>
<author><name sortKey="Berg, De" uniqKey="Berg D">DE Berg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dumrese, C" uniqKey="Dumrese C">C Dumrese</name>
</author>
<author><name sortKey="Slomianka, L" uniqKey="Slomianka L">L Slomianka</name>
</author>
<author><name sortKey="Ziegler, U" uniqKey="Ziegler U">U Ziegler</name>
</author>
<author><name sortKey="Choi, Ss" uniqKey="Choi S">SS Choi</name>
</author>
<author><name sortKey="Kalia, A" uniqKey="Kalia A">A Kalia</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bloor, Ae" uniqKey="Bloor A">AE Bloor</name>
</author>
<author><name sortKey="Cranenburgh, Rm" uniqKey="Cranenburgh R">RM Cranenburgh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Styer, Cm" uniqKey="Styer C">CM Styer</name>
</author>
<author><name sortKey="Hansen, Lm" uniqKey="Hansen L">LM Hansen</name>
</author>
<author><name sortKey="Cooke, Cl" uniqKey="Cooke C">CL Cooke</name>
</author>
<author><name sortKey="Gundersen, Am" uniqKey="Gundersen A">AM Gundersen</name>
</author>
<author><name sortKey="Choi, Ss" uniqKey="Choi S">SS Choi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Langford, Ml" uniqKey="Langford M">ML Langford</name>
</author>
<author><name sortKey="Zabaleta, J" uniqKey="Zabaleta J">J Zabaleta</name>
</author>
<author><name sortKey="Ochoa, Ac" uniqKey="Ochoa A">AC Ochoa</name>
</author>
<author><name sortKey="Testerman, Tl" uniqKey="Testerman T">TL Testerman</name>
</author>
<author><name sortKey="Mcgee, Dj" uniqKey="Mcgee D">DJ McGee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Benghezal, M" uniqKey="Benghezal M">M Benghezal</name>
</author>
<author><name sortKey="Dieye, Y" uniqKey="Dieye Y">Y Dieye</name>
</author>
<author><name sortKey="Schwan, C" uniqKey="Schwan C">C Schwan</name>
</author>
<author><name sortKey="Sehnal, M" uniqKey="Sehnal M">M Sehnal</name>
</author>
<author><name sortKey="Fulurija, A" uniqKey="Fulurija A">A Fulurija</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shevchuk, Na" uniqKey="Shevchuk N">NA Shevchuk</name>
</author>
<author><name sortKey="Bryksin, Av" uniqKey="Bryksin A">AV Bryksin</name>
</author>
<author><name sortKey="Nusinovich, Ya" uniqKey="Nusinovich Y">YA Nusinovich</name>
</author>
<author><name sortKey="Cabello, Fc" uniqKey="Cabello F">FC Cabello</name>
</author>
<author><name sortKey="Sutherland, M" uniqKey="Sutherland M">M Sutherland</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schoep, Td" uniqKey="Schoep T">TD Schoep</name>
</author>
<author><name sortKey="Fulurija, A" uniqKey="Fulurija A">A Fulurija</name>
</author>
<author><name sortKey="Good, F" uniqKey="Good F">F Good</name>
</author>
<author><name sortKey="Lu, W" uniqKey="Lu W">W Lu</name>
</author>
<author><name sortKey="Himbeck, Rp" uniqKey="Himbeck R">RP Himbeck</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Takeshita, S" uniqKey="Takeshita S">S Takeshita</name>
</author>
<author><name sortKey="Sato, M" uniqKey="Sato M">M Sato</name>
</author>
<author><name sortKey="Toba, M" uniqKey="Toba M">M Toba</name>
</author>
<author><name sortKey="Masahashi, W" uniqKey="Masahashi W">W Masahashi</name>
</author>
<author><name sortKey="Hashimoto Gotoh, T" uniqKey="Hashimoto Gotoh T">T Hashimoto-Gotoh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zakrzewska Czerwinska, J" uniqKey="Zakrzewska Czerwinska J">J Zakrzewska-Czerwinska</name>
</author>
<author><name sortKey="Jakimowicz, D" uniqKey="Jakimowicz D">D Jakimowicz</name>
</author>
<author><name sortKey="Zawilak Pawlik, A" uniqKey="Zawilak Pawlik A">A Zawilak-Pawlik</name>
</author>
<author><name sortKey="Messer, W" uniqKey="Messer W">W Messer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Olczak, Aa" uniqKey="Olczak A">AA Olczak</name>
</author>
<author><name sortKey="Olson, Jw" uniqKey="Olson J">JW Olson</name>
</author>
<author><name sortKey="Maier, Rj" uniqKey="Maier R">RJ Maier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tamura, K" uniqKey="Tamura K">K Tamura</name>
</author>
<author><name sortKey="Dudley, J" uniqKey="Dudley J">J Dudley</name>
</author>
<author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name>
</author>
<author><name sortKey="Kumar, S" uniqKey="Kumar S">S Kumar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Saitou, N" uniqKey="Saitou N">N Saitou</name>
</author>
<author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name>
</author>
<author><name sortKey="Lutkenhaus, J" uniqKey="Lutkenhaus J">J Lutkenhaus</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Michel, B" uniqKey="Michel B">B Michel</name>
</author>
<author><name sortKey="Recchia, Gd" uniqKey="Recchia G">GD Recchia</name>
</author>
<author><name sortKey="Penel Colin, M" uniqKey="Penel Colin M">M Penel-Colin</name>
</author>
<author><name sortKey="Ehrlich, Sd" uniqKey="Ehrlich S">SD Ehrlich</name>
</author>
<author><name sortKey="Sherratt, Dj" uniqKey="Sherratt D">DJ Sherratt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kang, J" uniqKey="Kang J">J Kang</name>
</author>
<author><name sortKey="Blaser, Mj" uniqKey="Blaser M">MJ Blaser</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Cr" uniqKey="Chen C">CR Chen</name>
</author>
<author><name sortKey="Malik, M" uniqKey="Malik M">M Malik</name>
</author>
<author><name sortKey="Snyder, M" uniqKey="Snyder M">M Snyder</name>
</author>
<author><name sortKey="Drlica, K" uniqKey="Drlica K">K Drlica</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zawilak Pawlik, A" uniqKey="Zawilak Pawlik A">A Zawilak-Pawlik</name>
</author>
<author><name sortKey="Kois, A" uniqKey="Kois A">A Kois</name>
</author>
<author><name sortKey="Stingl, K" uniqKey="Stingl K">K Stingl</name>
</author>
<author><name sortKey="Boneca, Ig" uniqKey="Boneca I">IG Boneca</name>
</author>
<author><name sortKey="Skrobuk, P" uniqKey="Skrobuk P">P Skrobuk</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kuempel, Pl" uniqKey="Kuempel P">PL Kuempel</name>
</author>
<author><name sortKey="Henson, Jm" uniqKey="Henson J">JM Henson</name>
</author>
<author><name sortKey="Dircks, L" uniqKey="Dircks L">L Dircks</name>
</author>
<author><name sortKey="Tecklenburg, M" uniqKey="Tecklenburg M">M Tecklenburg</name>
</author>
<author><name sortKey="Lim, Df" uniqKey="Lim D">DF Lim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nolivos, S" uniqKey="Nolivos S">S Nolivos</name>
</author>
<author><name sortKey="Pages, C" uniqKey="Pages C">C Pages</name>
</author>
<author><name sortKey="Rousseau, P" uniqKey="Rousseau P">P Rousseau</name>
</author>
<author><name sortKey="Le Bourgeois, P" uniqKey="Le Bourgeois P">P Le Bourgeois</name>
</author>
<author><name sortKey="Cornet, F" uniqKey="Cornet F">F Cornet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Recchia, Gd" uniqKey="Recchia G">GD Recchia</name>
</author>
<author><name sortKey="Aroyo, M" uniqKey="Aroyo M">M Aroyo</name>
</author>
<author><name sortKey="Wolf, D" uniqKey="Wolf D">D Wolf</name>
</author>
<author><name sortKey="Blakely, G" uniqKey="Blakely G">G Blakely</name>
</author>
<author><name sortKey="Sherratt, Dj" uniqKey="Sherratt D">DJ Sherratt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Val, Me" uniqKey="Val M">ME Val</name>
</author>
<author><name sortKey="Kennedy, Sp" uniqKey="Kennedy S">SP Kennedy</name>
</author>
<author><name sortKey="El Karoui, M" uniqKey="El Karoui M">M El Karoui</name>
</author>
<author><name sortKey="Bonne, L" uniqKey="Bonne L">L Bonne</name>
</author>
<author><name sortKey="Chevalier, F" uniqKey="Chevalier F">F Chevalier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yates, J" uniqKey="Yates J">J Yates</name>
</author>
<author><name sortKey="Aroyo, M" uniqKey="Aroyo M">M Aroyo</name>
</author>
<author><name sortKey="Sherratt, Dj" uniqKey="Sherratt D">DJ Sherratt</name>
</author>
<author><name sortKey="Barre, Fx" uniqKey="Barre F">FX Barre</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Blakely, Gw" uniqKey="Blakely G">GW Blakely</name>
</author>
<author><name sortKey="Davidson, Ao" uniqKey="Davidson A">AO Davidson</name>
</author>
<author><name sortKey="Sherratt, Dj" uniqKey="Sherratt D">DJ Sherratt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Manjunatha, Uh" uniqKey="Manjunatha U">UH Manjunatha</name>
</author>
<author><name sortKey="Dalal, M" uniqKey="Dalal M">M Dalal</name>
</author>
<author><name sortKey="Chatterji, M" uniqKey="Chatterji M">M Chatterji</name>
</author>
<author><name sortKey="Radha, Dr" uniqKey="Radha D">DR Radha</name>
</author>
<author><name sortKey="Visweswariah, Ss" uniqKey="Visweswariah S">SS Visweswariah</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Aubry, A" uniqKey="Aubry A">A Aubry</name>
</author>
<author><name sortKey="Fisher, Lm" uniqKey="Fisher L">LM Fisher</name>
</author>
<author><name sortKey="Jarlier, V" uniqKey="Jarlier V">V Jarlier</name>
</author>
<author><name sortKey="Cambau, E" uniqKey="Cambau E">E Cambau</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nurse, P" uniqKey="Nurse P">P Nurse</name>
</author>
<author><name sortKey="Levine, C" uniqKey="Levine C">C Levine</name>
</author>
<author><name sortKey="Hassing, H" uniqKey="Hassing H">H Hassing</name>
</author>
<author><name sortKey="Marians, Kj" uniqKey="Marians K">KJ Marians</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bigot, S" uniqKey="Bigot S">S Bigot</name>
</author>
<author><name sortKey="Saleh, Oa" uniqKey="Saleh O">OA Saleh</name>
</author>
<author><name sortKey="Lesterlin, C" uniqKey="Lesterlin C">C Lesterlin</name>
</author>
<author><name sortKey="Pages, C" uniqKey="Pages C">C Pages</name>
</author>
<author><name sortKey="El Karoui, M" uniqKey="El Karoui M">M El Karoui</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id>
<journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id>
<journal-id journal-id-type="publisher-id">plos</journal-id>
<journal-id journal-id-type="pmc">plosone</journal-id>
<journal-title-group><journal-title>PLoS ONE</journal-title>
</journal-title-group>
<issn pub-type="epub">1932-6203</issn>
<publisher><publisher-name>Public Library of Science</publisher-name>
<publisher-loc>San Francisco, USA</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">22511919</article-id>
<article-id pub-id-type="pmc">3325230</article-id>
<article-id pub-id-type="publisher-id">PONE-D-11-25692</article-id>
<article-id pub-id-type="doi">10.1371/journal.pone.0033310</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
<subj-group subj-group-type="Discipline-v2"><subject>Biology</subject>
<subj-group><subject>Computational Biology</subject>
<subj-group><subject>Genomics</subject>
<subj-group><subject>Genome Analysis Tools</subject>
<subj-group><subject>Genome Scans</subject>
<subject>Genome-Wide Association Studies</subject>
</subj-group>
</subj-group>
</subj-group>
<subj-group><subject>Molecular Genetics</subject>
<subj-group><subject>Gene Identification and Analysis</subject>
</subj-group>
</subj-group>
</subj-group>
<subj-group><subject>Molecular Cell Biology</subject>
<subj-group><subject>Nucleic Acids</subject>
<subj-group><subject>DNA</subject>
<subj-group><subject>DNA recombination</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
<subj-group subj-group-type="Discipline-v2"><subject>Medicine</subject>
<subj-group><subject>Infectious Diseases</subject>
<subj-group><subject>Bacterial Diseases</subject>
<subj-group><subject>Helicobacter Pylori</subject>
</subj-group>
</subj-group>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Xer Recombinase and Genome Integrity in <italic>Helicobacter pylori</italic>
, a Pathogen without Topoisomerase IV</article-title>
<alt-title alt-title-type="running-head">Xer Recombination and Genome Integrity</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Debowski</surname>
<given-names>Aleksandra W.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Carnoy</surname>
<given-names>Christophe</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Verbrugghe</surname>
<given-names>Phebe</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Nilsson</surname>
<given-names>Hans-Olof</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gauntlett</surname>
<given-names>Jonathan C.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Fulurija</surname>
<given-names>Alma</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Camilleri</surname>
<given-names>Tania</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Berg</surname>
<given-names>Douglas E.</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Marshall</surname>
<given-names>Barry J.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Benghezal</surname>
<given-names>Mohammed</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label>
<addr-line>Ondek Pty Ltd and Helicobacter pylori Research Laboratory, School of Pathology & Laboratory Medicine, M504, Marshall Centre for Infectious Disease Research and Training, University of Western Australia, Nedlands, Washington,</addr-line>
</aff>
<aff id="aff2"><label>2</label>
<addr-line>United States of America Center for Infection and Immunity of Lille, INSERM U 1019, CNRS UMR 8204, Univ Lille Nord de France, Institut Pasteur de Lille, Lille, France</addr-line>
</aff>
<aff id="aff3"><label>3</label>
<addr-line>Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, United States of America</addr-line>
</aff>
<contrib-group><contrib contrib-type="editor"><name><surname>Ahmed</surname>
<given-names>Niyaz</given-names>
</name>
<role>Editor</role>
<xref ref-type="aff" rid="edit1"></xref>
</contrib>
</contrib-group>
<aff id="edit1">University of Hyderabad, India</aff>
<author-notes><corresp id="cor1">* E-mail: <email>mbenghezal@ondek.com</email>
</corresp>
<fn fn-type="con"><p>Conceived and designed the experiments: AWD CC AF DEB BJM MB. Performed the experiments: AWD CC PV HN JCG TC MB. Analyzed the data: AWD CC PV HN DEB BJM MB. Contributed reagents/materials/analysis tools: AWD CC HN. Wrote the paper: AWD CC DEB MB.</p>
</fn>
</author-notes>
<pub-date pub-type="collection"><year>2012</year>
</pub-date>
<pub-date pub-type="epub"><day>12</day>
<month>4</month>
<year>2012</year>
</pub-date>
<volume>7</volume>
<issue>4</issue>
<elocation-id>e33310</elocation-id>
<history><date date-type="received"><day>15</day>
<month>12</month>
<year>2011</year>
</date>
<date date-type="accepted"><day>7</day>
<month>2</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>Debowski et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</copyright-statement>
<copyright-year>2012</copyright-year>
</permissions>
<abstract><p>In the model organism <italic>E. coli</italic>
, recombination mediated by the related XerC and XerD recombinases complexed with the FtsK translocase at specialized <italic>dif</italic>
sites, resolves dimeric chromosomes into free monomers to allow efficient chromosome segregation at cell division. Computational genome analysis of <italic>Helicobacter pylori</italic>
, a slow growing gastric pathogen, identified just one chromosomal <italic>xer</italic>
gene (<italic>xerH</italic>
) and its cognate <italic>dif</italic>
site (<italic>difH</italic>
). Here we show that recombination between directly repeated <italic>difH</italic>
sites requires XerH, FtsK but not XerT, the TnPZ transposon associated recombinase. <italic>xerH</italic>
inactivation was not lethal, but resulted in increased DNA per cell, suggesting defective chromosome segregation. The <italic>xerH</italic>
mutant also failed to colonize mice, and was more susceptible to UV and ciprofloxacin, which induce DNA breakage, and thereby recombination and chromosome dimer formation. <italic>xerH</italic>
inactivation and overexpression each led to a DNA segregation defect, suggesting a role for Xer recombination in regulation of replication. In addition to chromosome dimer resolution and based on the absence of genes for topoisomerase IV (<italic>parC</italic>
, <italic>parE</italic>
) in <italic>H. pylori</italic>
, we speculate that XerH may contribute to chromosome decatenation, although possible involvement of <italic>H. pylori</italic>
's DNA gyrase and topoisomerase III homologue are also considered. Further analyses of this system should contribute to general understanding of and possibly therapy development for <italic>H. pylori</italic>
, which causes peptic ulcers and gastric cancer; for the closely related, diarrheagenic Campylobacter species; and for unrelated slow growing pathogens that lack topoisomerase IV, such as <italic>Mycobacterium tuberculosis</italic>
.</p>
</abstract>
<counts><page-count count="15"></page-count>
</counts>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002859 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 002859 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:3325230 |texte= Xer Recombinase and Genome Integrity in Helicobacter pylori, a Pathogen without Topoisomerase IV }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:22511919" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a AustralieFrV1
This area was generated with Dilib version V0.6.33. |