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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Novel roles for KLF1 in erythropoiesis revealed by mRNA-seq</title><author><name sortKey="Tallack, Michael R" sort="Tallack, Michael R" uniqKey="Tallack M" first="Michael R." last="Tallack">Michael R. Tallack</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation></author><author><name sortKey="Magor, Graham W" sort="Magor, Graham W" uniqKey="Magor G" first="Graham W." last="Magor">Graham W. Magor</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation></author><author><name sortKey="Dartigues, Benjamin" sort="Dartigues, Benjamin" uniqKey="Dartigues B" first="Benjamin" last="Dartigues">Benjamin Dartigues</name><affiliation><nlm:aff id="aff2">Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">University of Bordeaux, 33400 Talence, France;</nlm:aff></affiliation></author><author><name sortKey="Sun, Lei" sort="Sun, Lei" uniqKey="Sun L" first="Lei" last="Sun">Lei Sun</name><affiliation><nlm:aff id="aff2">Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">School of Information and Electrical Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221008, China;</nlm:aff></affiliation></author><author><name sortKey="Huang, Stephen" sort="Huang, Stephen" uniqKey="Huang S" first="Stephen" last="Huang">Stephen Huang</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">School of Biomedical Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation></author><author><name sortKey="Fittock, Jessica M" sort="Fittock, Jessica M" uniqKey="Fittock J" first="Jessica M." last="Fittock">Jessica M. Fittock</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">School of Biomedical Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation></author><author><name sortKey="Fry, Sally V" sort="Fry, Sally V" uniqKey="Fry S" first="Sally V." last="Fry">Sally V. Fry</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation></author><author><name sortKey="Glazov, Evgeny A" sort="Glazov, Evgeny A" uniqKey="Glazov E" first="Evgeny A." last="Glazov">Evgeny A. Glazov</name><affiliation><nlm:aff id="aff6">The University of Queensland Diamantina Institute, The University of Queensland, Brisbane, Queensland 4102, Australia;</nlm:aff></affiliation></author><author><name sortKey="Bailey, Timothy L" sort="Bailey, Timothy L" uniqKey="Bailey T" first="Timothy L." last="Bailey">Timothy L. Bailey</name><affiliation><nlm:aff id="aff2">Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation></author><author><name sortKey="Perkins, Andrew C" sort="Perkins, Andrew C" uniqKey="Perkins A" first="Andrew C." last="Perkins">Andrew C. Perkins</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">School of Biomedical Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff7">The Princess Alexandra Hospital, Brisbane, Queensland 4102, Australia</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22835905</idno><idno type="pmc">3514668</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3514668</idno><idno type="RBID">PMC:3514668</idno><idno type="doi">10.1101/gr.135707.111</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">001847</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001847</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Novel roles for KLF1 in erythropoiesis revealed by mRNA-seq</title><author><name sortKey="Tallack, Michael R" sort="Tallack, Michael R" uniqKey="Tallack M" first="Michael R." last="Tallack">Michael R. Tallack</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation></author><author><name sortKey="Magor, Graham W" sort="Magor, Graham W" uniqKey="Magor G" first="Graham W." last="Magor">Graham W. Magor</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation></author><author><name sortKey="Dartigues, Benjamin" sort="Dartigues, Benjamin" uniqKey="Dartigues B" first="Benjamin" last="Dartigues">Benjamin Dartigues</name><affiliation><nlm:aff id="aff2">Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">University of Bordeaux, 33400 Talence, France;</nlm:aff></affiliation></author><author><name sortKey="Sun, Lei" sort="Sun, Lei" uniqKey="Sun L" first="Lei" last="Sun">Lei Sun</name><affiliation><nlm:aff id="aff2">Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">School of Information and Electrical Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221008, China;</nlm:aff></affiliation></author><author><name sortKey="Huang, Stephen" sort="Huang, Stephen" uniqKey="Huang S" first="Stephen" last="Huang">Stephen Huang</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">School of Biomedical Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation></author><author><name sortKey="Fittock, Jessica M" sort="Fittock, Jessica M" uniqKey="Fittock J" first="Jessica M." last="Fittock">Jessica M. Fittock</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">School of Biomedical Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation></author><author><name sortKey="Fry, Sally V" sort="Fry, Sally V" uniqKey="Fry S" first="Sally V." last="Fry">Sally V. Fry</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation></author><author><name sortKey="Glazov, Evgeny A" sort="Glazov, Evgeny A" uniqKey="Glazov E" first="Evgeny A." last="Glazov">Evgeny A. Glazov</name><affiliation><nlm:aff id="aff6">The University of Queensland Diamantina Institute, The University of Queensland, Brisbane, Queensland 4102, Australia;</nlm:aff></affiliation></author><author><name sortKey="Bailey, Timothy L" sort="Bailey, Timothy L" uniqKey="Bailey T" first="Timothy L." last="Bailey">Timothy L. Bailey</name><affiliation><nlm:aff id="aff2">Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation></author><author><name sortKey="Perkins, Andrew C" sort="Perkins, Andrew C" uniqKey="Perkins A" first="Andrew C." last="Perkins">Andrew C. Perkins</name><affiliation><nlm:aff id="aff1">Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">School of Biomedical Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia;</nlm:aff></affiliation><affiliation><nlm:aff id="aff7">The Princess Alexandra Hospital, Brisbane, Queensland 4102, Australia</nlm:aff></affiliation></author></analytic><series><title level="j">Genome Research</title><idno type="ISSN">1088-9051</idno><idno type="eISSN">1549-5469</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>KLF1 (formerly known as EKLF) regulates the development of erythroid cells from bi-potent progenitor cells via the transcriptional activation of a diverse set of genes. Mice lacking <italic>Klf1</italic> die in utero prior to E15 from severe anemia due to the inadequate expression of genes controlling hemoglobin production, cell membrane and cytoskeletal integrity, and the cell cycle. We have recently described the full repertoire of KLF1 binding sites in vivo by performing KLF1 ChIP-seq in primary erythroid tissue (E14.5 fetal liver). Here we describe the KLF1-dependent erythroid transcriptome by comparing mRNA-seq from <italic>Klf1<sup>+/+</sup></italic> and <italic>Klf1<sup>−/−</sup></italic> erythroid tissue. This has revealed novel target genes not previously obtainable by traditional microarray technology, and provided novel insights into the function of KLF1 as a transcriptional activator. We define a <italic>cis</italic>-regulatory module bound by KLF1, GATA1, TAL1, and EP300 that coordinates a core set of erythroid genes. We also describe a novel set of erythroid-specific promoters that drive high-level expression of otherwise ubiquitously expressed genes in erythroid cells. Our study has identified two novel lncRNAs that are dynamically expressed during erythroid differentiation, and discovered a role for KLF1 in directing apoptotic gene expression to drive the terminal stages of erythroid maturation.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Genome Res</journal-id><journal-id journal-id-type="iso-abbrev">Genome Res</journal-id><journal-id journal-id-type="publisher-id">GENOME</journal-id><journal-title-group><journal-title>Genome Research</journal-title></journal-title-group><issn pub-type="ppub">1088-9051</issn><issn pub-type="epub">1549-5469</issn><publisher><publisher-name>Cold Spring Harbor Laboratory Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22835905</article-id><article-id pub-id-type="pmc">3514668</article-id><article-id pub-id-type="medline">9518021</article-id><article-id pub-id-type="doi">10.1101/gr.135707.111</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research</subject></subj-group></article-categories><title-group><article-title>Novel roles for KLF1 in erythropoiesis revealed by mRNA-seq</article-title><alt-title alt-title-type="left-running">Tallack et al.</alt-title><alt-title alt-title-type="right-running">The KLF1-dependent erythroid transcriptome</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Tallack</surname><given-names>Michael R.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Magor</surname><given-names>Graham W.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Dartigues</surname><given-names>Benjamin</given-names></name><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Sun</surname><given-names>Lei</given-names></name><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Stephen</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff5">5</xref></contrib><contrib contrib-type="author"><name><surname>Fittock</surname><given-names>Jessica M.</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff5">5</xref></contrib><contrib contrib-type="author"><name><surname>Fry</surname><given-names>Sally V.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Glazov</surname><given-names>Evgeny A.</given-names></name><xref ref-type="aff" rid="aff6">6</xref></contrib><contrib contrib-type="author"><name><surname>Bailey</surname><given-names>Timothy L.</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Perkins</surname><given-names>Andrew C.</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff5">5</xref><xref ref-type="aff" rid="aff7">7</xref><xref ref-type="corresp" rid="cor1">8</xref></contrib><aff id="aff1"><label>1</label>Mater Medical Research Institute, Mater Hospital, Brisbane, Queensland 4101, Australia;</aff><aff id="aff2"><label>2</label>Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia;</aff><aff id="aff3"><label>3</label>University of Bordeaux, 33400 Talence, France;</aff><aff id="aff4"><label>4</label>School of Information and Electrical Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221008, China;</aff><aff id="aff5"><label>5</label>School of Biomedical Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia;</aff><aff id="aff6"><label>6</label>The University of Queensland Diamantina Institute, The University of Queensland, Brisbane, Queensland 4102, Australia;</aff><aff id="aff7"><label>7</label>The Princess Alexandra Hospital, Brisbane, Queensland 4102, Australia</aff></contrib-group><author-notes><corresp id="cor1"><label>8</label>Corresponding author E-mail <email xlink:type="simple">aperkins@mmri.mater.org.au</email></corresp></author-notes><pub-date pub-type="ppub"><month>12</month><year>2012</year></pub-date><volume>22</volume><issue>12</issue><fpage>2385</fpage><lpage>2398</lpage><history><date date-type="received"><day>1</day><month>12</month><year>2011</year></date><date date-type="accepted"><day>23</day><month>7</month><year>2012</year></date></history><permissions><copyright-statement><ext-link ext-link-type="uri" xlink:href="http://www.genome.org/site/misc/terms.xhtml">© 2012, Published by Cold Spring Harbor Laboratory Press</ext-link></copyright-statement><copyright-year>2012</copyright-year><license license-type="creative-commons" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/"><license-p>This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see <ext-link ext-link-type="uri" xlink:href="http://genome.cshlp.org/site/misc/terms.xhtml">http://genome.cshlp.org/site/misc/terms.xhtml</ext-link>). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0/</ext-link>.</license-p></license></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="2385.pdf"></self-uri><abstract><p>KLF1 (formerly known as EKLF) regulates the development of erythroid cells from bi-potent progenitor cells via the transcriptional activation of a diverse set of genes. Mice lacking <italic>Klf1</italic> die in utero prior to E15 from severe anemia due to the inadequate expression of genes controlling hemoglobin production, cell membrane and cytoskeletal integrity, and the cell cycle. We have recently described the full repertoire of KLF1 binding sites in vivo by performing KLF1 ChIP-seq in primary erythroid tissue (E14.5 fetal liver). Here we describe the KLF1-dependent erythroid transcriptome by comparing mRNA-seq from <italic>Klf1<sup>+/+</sup></italic> and <italic>Klf1<sup>−/−</sup></italic> erythroid tissue. This has revealed novel target genes not previously obtainable by traditional microarray technology, and provided novel insights into the function of KLF1 as a transcriptional activator. We define a <italic>cis</italic>-regulatory module bound by KLF1, GATA1, TAL1, and EP300 that coordinates a core set of erythroid genes. We also describe a novel set of erythroid-specific promoters that drive high-level expression of otherwise ubiquitously expressed genes in erythroid cells. Our study has identified two novel lncRNAs that are dynamically expressed during erythroid differentiation, and discovered a role for KLF1 in directing apoptotic gene expression to drive the terminal stages of erythroid maturation.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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