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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Two Loci Control Phytoglycogen Production in the Monocellular
Green Alga <italic>Chlamydomonas reinhardtii</italic><xref ref-type="fn" rid="FN1">1</xref></title><author><name sortKey="Dauvillee, David" sort="Dauvillee, David" uniqKey="Dauvillee D" first="David" last="Dauvillée">David Dauvillée</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Colleoni, Christophe" sort="Colleoni, Christophe" uniqKey="Colleoni C" first="Christophe" last="Colleoni">Christophe Colleoni</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Mouille, Gregory" sort="Mouille, Gregory" uniqKey="Mouille G" first="Gregory" last="Mouille">Gregory Mouille</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Buleon, Alain" sort="Buleon, Alain" uniqKey="Buleon A" first="Alain" last="Buléon">Alain Buléon</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Gallant, Daniel J" sort="Gallant, Daniel J" uniqKey="Gallant D" first="Daniel J." last="Gallant">Daniel J. Gallant</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Bouchet, Brigitte" sort="Bouchet, Brigitte" uniqKey="Bouchet B" first="Brigitte" last="Bouchet">Brigitte Bouchet</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Morell, Matthew K" sort="Morell, Matthew K" uniqKey="Morell M" first="Matthew K." last="Morell">Matthew K. Morell</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="D Hulst, Christophe" sort="D Hulst, Christophe" uniqKey="D Hulst C" first="Christophe" last="D'Hulst">Christophe D'Hulst</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Myers, Alan M" sort="Myers, Alan M" uniqKey="Myers A" first="Alan M." last="Myers">Alan M. Myers</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Ball, Steven G" sort="Ball, Steven G" uniqKey="Ball S" first="Steven G." last="Ball">Steven G. Ball</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">11299352</idno><idno type="pmc">88828</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC88828</idno><idno type="RBID">PMC:88828</idno><date when="2001">2001</date><idno type="wicri:Area/Pmc/Corpus">000A12</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000A12</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Two Loci Control Phytoglycogen Production in the Monocellular
Green Alga <italic>Chlamydomonas reinhardtii</italic><xref ref-type="fn" rid="FN1">1</xref></title><author><name sortKey="Dauvillee, David" sort="Dauvillee, David" uniqKey="Dauvillee D" first="David" last="Dauvillée">David Dauvillée</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Colleoni, Christophe" sort="Colleoni, Christophe" uniqKey="Colleoni C" first="Christophe" last="Colleoni">Christophe Colleoni</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Mouille, Gregory" sort="Mouille, Gregory" uniqKey="Mouille G" first="Gregory" last="Mouille">Gregory Mouille</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Buleon, Alain" sort="Buleon, Alain" uniqKey="Buleon A" first="Alain" last="Buléon">Alain Buléon</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Gallant, Daniel J" sort="Gallant, Daniel J" uniqKey="Gallant D" first="Daniel J." last="Gallant">Daniel J. Gallant</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Bouchet, Brigitte" sort="Bouchet, Brigitte" uniqKey="Bouchet B" first="Brigitte" last="Bouchet">Brigitte Bouchet</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Morell, Matthew K" sort="Morell, Matthew K" uniqKey="Morell M" first="Matthew K." last="Morell">Matthew K. Morell</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="D Hulst, Christophe" sort="D Hulst, Christophe" uniqKey="D Hulst C" first="Christophe" last="D'Hulst">Christophe D'Hulst</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Myers, Alan M" sort="Myers, Alan M" uniqKey="Myers A" first="Alan M." last="Myers">Alan M. Myers</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Ball, Steven G" sort="Ball, Steven G" uniqKey="Ball S" first="Steven G." last="Ball">Steven G. Ball</name><affiliation><nlm:aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The <italic>STA8</italic> locus of <italic>Chlamydomonas
reinhardtii</italic> was identified in a genetic screen as a factor that
controls starch biosynthesis. Mutations of <italic>STA8</italic> cause a
significant reduction in the amount of granular starch produced during
nutrient limitation and accumulate phytoglycogen. The granules
remaining in <italic>sta8</italic> mutants are misshapen, and the
abundance of amylose and long chains in amylopectin is altered.
Mutations of the <italic>STA7</italic> locus, which completely lack
isoamylase activity, also cause accumulation of phytoglycogen, although
<italic>sta8</italic> and <italic>sta7</italic> mutants differ in that
there is a complete loss of granular starch in the latter. This is the
first instance in which mutations of two different genetic elements in
one plant species have been shown to cause phytoglycogen accumulation.
An analytical procedure that allows assay of isoamylase in total
extracts was developed and used to show that <italic>sta8</italic>mutations cause a 65% reduction in the level of this activity. All
other enzymes known to be involved in starch biosynthesis were shown to
be unaffected in <italic>sta8</italic> mutants. The same amount of total
isoamylase activity (approximately) as that present in
<italic>sta8</italic> mutants was observed in heterozygous triploids
containing two <italic>sta7</italic> mutant alleles and one wild-type
allele. This strain, however, accumulates normal levels of starch
granules and lacks phytoglycogen. The total level of isoamylase
activity, therefore, is not the major determinant of whether granule
production is reduced and phytoglycogen accumulates. Instead, a
qualitative property of the isoamylase that is affected by the
<italic>sta8</italic> mutation is likely to be the critical factor in
phytoglycogen production.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="publisher-id">PLANT PHYSIOL</journal-id><journal-title>Plant Physiology</journal-title><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Physiologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">11299352</article-id><article-id pub-id-type="pmc">88828</article-id><article-id pub-id-type="publisher-id">6565</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject><subj-group><subject>Biochemical Processes and Macromolecular Structures</subject></subj-group></subj-group></article-categories><title-group><article-title>Two Loci Control Phytoglycogen Production in the Monocellular
Green Alga <italic>Chlamydomonas reinhardtii</italic><xref ref-type="fn" rid="FN1">1</xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Dauvillée</surname><given-names>David</given-names></name><xref ref-type="aff" rid="N0x8a7a9c8N0xa031190"></xref></contrib><contrib contrib-type="author"><name><surname>Colleoni</surname><given-names>Christophe</given-names></name><xref ref-type="aff" rid="N0x8a7a9c8N0xa031190"></xref></contrib><contrib contrib-type="author"><name><surname>Mouille</surname><given-names>Gregory</given-names></name><xref ref-type="aff" rid="N0x8a7a9c8N0xa031190"></xref></contrib><contrib contrib-type="author"><name><surname>Buléon</surname><given-names>Alain</given-names></name><xref ref-type="aff" rid="N0x8a7a9c8N0xa031190"></xref></contrib><contrib contrib-type="author"><name><surname>Gallant</surname><given-names>Daniel J.</given-names></name><xref ref-type="aff" rid="N0x8a7a9c8N0xa031190"></xref></contrib><contrib contrib-type="author"><name><surname>Bouchet</surname><given-names>Brigitte</given-names></name><xref ref-type="aff" rid="N0x8a7a9c8N0xa031190"></xref></contrib><contrib contrib-type="author"><name><surname>Morell</surname><given-names>Matthew K.</given-names></name><xref ref-type="aff" rid="N0x8a7a9c8N0xa031190"></xref></contrib><contrib contrib-type="author"><name><surname>d'Hulst</surname><given-names>Christophe</given-names></name><xref ref-type="aff" rid="N0x8a7a9c8N0xa031190"></xref></contrib><contrib contrib-type="author"><name><surname>Myers</surname><given-names>Alan M.</given-names></name><xref ref-type="aff" rid="N0x8a7a9c8N0xa031190"></xref></contrib><contrib contrib-type="author"><name><surname>Ball</surname><given-names>Steven G.</given-names></name><xref ref-type="author-notes" rid="FN150">*</xref><xref ref-type="aff" rid="N0x8a7a9c8N0xa031190"></xref></contrib></contrib-group><aff id="N0x8a7a9c8N0xa031190">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France (D.D., C.C., G.M., C.d.H., S.G.B.); Institut National de la Recherche Agronomique, Centre de Recherches Agroalimentaires, Rue de la Géraudière, Boîte Postale 71627, 44316 Nantes cedex 03, France (A.B., D.J.G., B.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</aff><author-notes><fn id="FN150"><label>*</label><p>Corresponding author; e-mail <email>steven.ball@univ-lille1.fr</email>; fax
33–3–20–43–65–55.</p></fn></author-notes><pub-date pub-type="ppub"><month>4</month><year>2001</year></pub-date><volume>125</volume><issue>4</issue><fpage>1710</fpage><lpage>1722</lpage><history><date date-type="received"><day>9</day><month>10</month><year>2000</year></date><date date-type="rev-recd"><day>30</day><month>11</month><year>2000</year></date><date date-type="accepted"><day>4</day><month>1</month><year>2001</year></date></history><copyright-statement>Copyright © 2001, American Society of Plant Physiologists</copyright-statement><copyright-year>2001</copyright-year><abstract><p>The <italic>STA8</italic> locus of <italic>Chlamydomonas
reinhardtii</italic> was identified in a genetic screen as a factor that
controls starch biosynthesis. Mutations of <italic>STA8</italic> cause a
significant reduction in the amount of granular starch produced during
nutrient limitation and accumulate phytoglycogen. The granules
remaining in <italic>sta8</italic> mutants are misshapen, and the
abundance of amylose and long chains in amylopectin is altered.
Mutations of the <italic>STA7</italic> locus, which completely lack
isoamylase activity, also cause accumulation of phytoglycogen, although
<italic>sta8</italic> and <italic>sta7</italic> mutants differ in that
there is a complete loss of granular starch in the latter. This is the
first instance in which mutations of two different genetic elements in
one plant species have been shown to cause phytoglycogen accumulation.
An analytical procedure that allows assay of isoamylase in total
extracts was developed and used to show that <italic>sta8</italic>mutations cause a 65% reduction in the level of this activity. All
other enzymes known to be involved in starch biosynthesis were shown to
be unaffected in <italic>sta8</italic> mutants. The same amount of total
isoamylase activity (approximately) as that present in
<italic>sta8</italic> mutants was observed in heterozygous triploids
containing two <italic>sta7</italic> mutant alleles and one wild-type
allele. This strain, however, accumulates normal levels of starch
granules and lacks phytoglycogen. The total level of isoamylase
activity, therefore, is not the major determinant of whether granule
production is reduced and phytoglycogen accumulates. Instead, a
qualitative property of the isoamylase that is affected by the
<italic>sta8</italic> mutation is likely to be the critical factor in
phytoglycogen production.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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