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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biochemical Characterization of Wild-Type and Mutant Isoamylases
of <italic>Chlamydomonas reinhardtii</italic> Supports a Function of the
Multimeric Enzyme Organization in Amylopectin
Maturation<xref ref-type="fn" rid="FN1">1</xref></title><author><name sortKey="Dauvillee, David" sort="Dauvillee, David" uniqKey="Dauvillee D" first="David" last="Dauvillée">David Dauvillée</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Colleoni, Christophe" sort="Colleoni, Christophe" uniqKey="Colleoni C" first="Christophe" last="Colleoni">Christophe Colleoni</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Mouille, Gregory" sort="Mouille, Gregory" uniqKey="Mouille G" first="Gregory" last="Mouille">Gregory Mouille</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Morell, Matthew K" sort="Morell, Matthew K" uniqKey="Morell M" first="Matthew K." last="Morell">Matthew K. Morell</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="D Hulst, Christophe" sort="D Hulst, Christophe" uniqKey="D Hulst C" first="Christophe" last="D'Hulst">Christophe D'Hulst</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Wattebled, Fabrice" sort="Wattebled, Fabrice" uniqKey="Wattebled F" first="Fabrice" last="Wattebled">Fabrice Wattebled</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Lienard, Luc" sort="Lienard, Luc" uniqKey="Lienard L" first="Luc" last="Liénard">Luc Liénard</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Delvalle, David" sort="Delvalle, David" uniqKey="Delvalle D" first="David" last="Delvallé">David Delvallé</name></author><author><name sortKey="Ral, Jean Philippe" sort="Ral, Jean Philippe" uniqKey="Ral J" first="Jean-Philippe" last="Ral">Jean-Philippe Ral</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Myers, Alan M" sort="Myers, Alan M" uniqKey="Myers A" first="Alan M." last="Myers">Alan M. Myers</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Ball, Steven G" sort="Ball, Steven G" uniqKey="Ball S" first="Steven G." last="Ball">Steven G. Ball</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">11299353</idno><idno type="pmc">88829</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC88829</idno><idno type="RBID">PMC:88829</idno><date when="2001">2001</date><idno type="wicri:Area/Pmc/Corpus">000A13</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000A13</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Biochemical Characterization of Wild-Type and Mutant Isoamylases
of <italic>Chlamydomonas reinhardtii</italic> Supports a Function of the
Multimeric Enzyme Organization in Amylopectin
Maturation<xref ref-type="fn" rid="FN1">1</xref></title><author><name sortKey="Dauvillee, David" sort="Dauvillee, David" uniqKey="Dauvillee D" first="David" last="Dauvillée">David Dauvillée</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Colleoni, Christophe" sort="Colleoni, Christophe" uniqKey="Colleoni C" first="Christophe" last="Colleoni">Christophe Colleoni</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Mouille, Gregory" sort="Mouille, Gregory" uniqKey="Mouille G" first="Gregory" last="Mouille">Gregory Mouille</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Morell, Matthew K" sort="Morell, Matthew K" uniqKey="Morell M" first="Matthew K." last="Morell">Matthew K. Morell</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="D Hulst, Christophe" sort="D Hulst, Christophe" uniqKey="D Hulst C" first="Christophe" last="D'Hulst">Christophe D'Hulst</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Wattebled, Fabrice" sort="Wattebled, Fabrice" uniqKey="Wattebled F" first="Fabrice" last="Wattebled">Fabrice Wattebled</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Lienard, Luc" sort="Lienard, Luc" uniqKey="Lienard L" first="Luc" last="Liénard">Luc Liénard</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Delvalle, David" sort="Delvalle, David" uniqKey="Delvalle D" first="David" last="Delvallé">David Delvallé</name></author><author><name sortKey="Ral, Jean Philippe" sort="Ral, Jean Philippe" uniqKey="Ral J" first="Jean-Philippe" last="Ral">Jean-Philippe Ral</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Myers, Alan M" sort="Myers, Alan M" uniqKey="Myers A" first="Alan M." last="Myers">Alan M. Myers</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author><author><name sortKey="Ball, Steven G" sort="Ball, Steven G" uniqKey="Ball S" first="Steven G." last="Ball">Steven G. Ball</name><affiliation><nlm:aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</nlm:aff></affiliation></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><italic>Chlamydomonas reinhardtii</italic> mutants of the
<italic>STA8</italic> gene produce reduced amounts of high amylose starch
and phytoglycogen. In contrast to the previously described
phytoglycogen-producing mutants of <italic>C. reinhardtii</italic> that
contain no residual isoamylase activity, the <italic>sta8</italic>mutants still contained 35% of the normal amount of enzyme activity.
We have purified this residual isoamylase and compared it with the
wild-type <italic>C. reinhardtii</italic> enzyme. We have found that the
high-mass multimeric enzyme has reduced its average mass at least by
one-half. This coincides with the disappearance of two out of the three
activity bands that can be seen on zymogram gels. Wild-type and mutant
enzymes are shown to be located within the plastid. In addition, they
both act by cleaving off the outer branches of polysaccharides with no
consistent difference in enzyme specificity. Because the mutant enzyme
was demonstrated to digest phytoglycogen to completion in vitro, we
propose that its inability to do so in vivo supports a function of the
enzyme complex architecture in the processing of pre-amylopectin
chains.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="publisher-id">PLANT PHYSIOL</journal-id><journal-title>Plant Physiology</journal-title><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Physiologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">11299353</article-id><article-id pub-id-type="pmc">88829</article-id><article-id pub-id-type="publisher-id">6566</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject><subj-group><subject>Biochemical Processes and Macromolecular Structures</subject></subj-group></subj-group></article-categories><title-group><article-title>Biochemical Characterization of Wild-Type and Mutant Isoamylases
of <italic>Chlamydomonas reinhardtii</italic> Supports a Function of the
Multimeric Enzyme Organization in Amylopectin
Maturation<xref ref-type="fn" rid="FN1">1</xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Dauvillée</surname><given-names>David</given-names></name><xref ref-type="aff" rid="N0xad01480N0xabe5780"></xref><xref ref-type="aff" rid="N0xad01480N0xabe5780"></xref></contrib><contrib contrib-type="author"><name><surname>Colleoni</surname><given-names>Christophe</given-names></name><xref ref-type="aff" rid="N0xad01480N0xabe5780"></xref></contrib><contrib contrib-type="author"><name><surname>Mouille</surname><given-names>Gregory</given-names></name><xref ref-type="aff" rid="N0xad01480N0xabe5780"></xref></contrib><contrib contrib-type="author"><name><surname>Morell</surname><given-names>Matthew K.</given-names></name><xref ref-type="aff" rid="N0xad01480N0xabe5780"></xref></contrib><contrib contrib-type="author"><name><surname>d'Hulst</surname><given-names>Christophe</given-names></name><xref ref-type="aff" rid="N0xad01480N0xabe5780"></xref></contrib><contrib contrib-type="author"><name><surname>Wattebled</surname><given-names>Fabrice</given-names></name><xref ref-type="aff" rid="N0xad01480N0xabe5780"></xref></contrib><contrib contrib-type="author"><name><surname>Liénard</surname><given-names>Luc</given-names></name><xref ref-type="aff" rid="N0xad01480N0xabe5780"></xref></contrib><contrib contrib-type="author"><name><surname>Delvallé</surname><given-names>David</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ral</surname><given-names>Jean-Philippe</given-names></name><xref ref-type="aff" rid="N0xad01480N0xabe5780"></xref></contrib><contrib contrib-type="author"><name><surname>Myers</surname><given-names>Alan M.</given-names></name><xref ref-type="aff" rid="N0xad01480N0xabe5780"></xref></contrib><contrib contrib-type="author"><name><surname>Ball</surname><given-names>Steven G.</given-names></name><xref ref-type="author-notes" rid="FN150">*</xref><xref ref-type="aff" rid="N0xad01480N0xabe5780"></xref></contrib></contrib-group><aff id="N0xad01480N0xabe5780">Laboratoire de Chimie Biologique, Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 8576, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'ascq cedex, France (D.D., C.C., G.M., C.d.H, F.W., L.L., D.D., J.-P.R., S.G.B.); Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (M.K.M.); and Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (A.M.M.)</aff><author-notes><fn id="FN150"><label>*</label><p>Corresponding author; e-mail <email>steven.ball@univ-lille1.fr</email>; fax
33–3–20–43–65–55.</p></fn></author-notes><pub-date pub-type="ppub"><month>4</month><year>2001</year></pub-date><volume>125</volume><issue>4</issue><fpage>1723</fpage><lpage>1731</lpage><history><date date-type="received"><day>9</day><month>10</month><year>2000</year></date><date date-type="rev-recd"><day>30</day><month>11</month><year>2000</year></date><date date-type="accepted"><day>4</day><month>1</month><year>2001</year></date></history><copyright-statement>Copyright © 2001, American Society of Plant Physiologists</copyright-statement><copyright-year>2001</copyright-year><abstract><p><italic>Chlamydomonas reinhardtii</italic> mutants of the
<italic>STA8</italic> gene produce reduced amounts of high amylose starch
and phytoglycogen. In contrast to the previously described
phytoglycogen-producing mutants of <italic>C. reinhardtii</italic> that
contain no residual isoamylase activity, the <italic>sta8</italic>mutants still contained 35% of the normal amount of enzyme activity.
We have purified this residual isoamylase and compared it with the
wild-type <italic>C. reinhardtii</italic> enzyme. We have found that the
high-mass multimeric enzyme has reduced its average mass at least by
one-half. This coincides with the disappearance of two out of the three
activity bands that can be seen on zymogram gels. Wild-type and mutant
enzymes are shown to be located within the plastid. In addition, they
both act by cleaving off the outer branches of polysaccharides with no
consistent difference in enzyme specificity. Because the mutant enzyme
was demonstrated to digest phytoglycogen to completion in vitro, we
propose that its inability to do so in vivo supports a function of the
enzyme complex architecture in the processing of pre-amylopectin
chains.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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