Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts
Identifieur interne : 000B89 ( Pmc/Checkpoint ); précédent : 000B88; suivant : 000B90Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts
Auteurs : David Morse [Australie] ; Wesley Webster [Australie] ; Ming Kalanon [Australie] ; Gordon Langsley [France] ; Geoffrey I. Mcfadden [Australie]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
Over-expression of a GFP-
Url:
DOI: 10.1371/journal.pone.0158174
PubMed: 27348424
PubMed Central: 4922565
Affiliations:
- Australie, France
- Victoria (État), Île-de-France
- Melbourne, Paris
- Université Paris-Descartes, Université de Melbourne
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PMC:4922565Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>Plasmodium falciparum</italic> Rab1A Localizes to Rhoptries in Schizonts</title><author><name sortKey="Morse, David" sort="Morse, David" uniqKey="Morse D" first="David" last="Morse">David Morse</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Webster, Wesley" sort="Webster, Wesley" uniqKey="Webster W" first="Wesley" last="Webster">Wesley Webster</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Kalanon, Ming" sort="Kalanon, Ming" uniqKey="Kalanon M" first="Ming" last="Kalanon">Ming Kalanon</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Langsley, Gordon" sort="Langsley, Gordon" uniqKey="Langsley G" first="Gordon" last="Langsley">Gordon Langsley</name><affiliation wicri:level="4"><nlm:aff id="aff002"><addr-line>Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, 75014, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, 75014, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName><orgName type="university">Université Paris-Descartes</orgName></affiliation></author><author><name sortKey="Mcfadden, Geoffrey I" sort="Mcfadden, Geoffrey I" uniqKey="Mcfadden G" first="Geoffrey I." last="Mcfadden">Geoffrey I. Mcfadden</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27348424</idno><idno type="pmc">4922565</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4922565</idno><idno type="RBID">PMC:4922565</idno><idno type="doi">10.1371/journal.pone.0158174</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000621</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000621</idno><idno type="wicri:Area/Pmc/Curation">000620</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000620</idno><idno type="wicri:Area/Pmc/Checkpoint">000B89</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000B89</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>Plasmodium falciparum</italic> Rab1A Localizes to Rhoptries in Schizonts</title><author><name sortKey="Morse, David" sort="Morse, David" uniqKey="Morse D" first="David" last="Morse">David Morse</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Webster, Wesley" sort="Webster, Wesley" uniqKey="Webster W" first="Wesley" last="Webster">Wesley Webster</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Kalanon, Ming" sort="Kalanon, Ming" uniqKey="Kalanon M" first="Ming" last="Kalanon">Ming Kalanon</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Langsley, Gordon" sort="Langsley, Gordon" uniqKey="Langsley G" first="Gordon" last="Langsley">Gordon Langsley</name><affiliation wicri:level="4"><nlm:aff id="aff002"><addr-line>Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, 75014, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, 75014, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName><orgName type="university">Université Paris-Descartes</orgName></affiliation></author><author><name sortKey="Mcfadden, Geoffrey I" sort="Mcfadden, Geoffrey I" uniqKey="Mcfadden G" first="Geoffrey I." last="Mcfadden">Geoffrey I. Mcfadden</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Over-expression of a GFP-<italic>Pf</italic>Rab1A fusion protein in <italic>Plasmodium falciparum</italic> schizonts produces a punctate pattern of fluorescence typical of rhoptries, secretory organelles involved in host cell invasion. The GFP-positive bodies were purified by a combination of differential and density gradient centrifugation and their protein content determined by MS/MS sequencing. Consistent with the GFP rhoptry-like pattern of transgenic parasites, four of the 19 proteins identified have been previously described to be rhoptry-associated and another four are ER or ER-associated proteins. Confirmation that GFP-<italic>Pf</italic>Rab1A decorates rhoptries was obtained by its co-localization with Rap1 and Ron4 in late phase schizonts. We conclude that <italic>Pf</italic>Rab1A potentially regulates vesicular traffic from the endoplasmic reticulum to the rhoptries in <italic>Apicomplexa</italic> parasites.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cowman, Af" uniqKey="Cowman A">AF Cowman</name></author><author><name sortKey="Crabb, Bs" uniqKey="Crabb B">BS Crabb</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Waller, Rf" uniqKey="Waller R">RF Waller</name></author><author><name sortKey="Mcfadden, Gi" uniqKey="Mcfadden G">GI McFadden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ralph, Sa" uniqKey="Ralph S">SA Ralph</name></author><author><name sortKey="Van Dooren, Gg" uniqKey="Van Dooren G">GG Van Dooren</name></author><author><name sortKey="Waller, Rf" uniqKey="Waller R">RF Waller</name></author><author><name sortKey="Crawford, Mj" uniqKey="Crawford M">MJ 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27348424</article-id><article-id pub-id-type="pmc">4922565</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0158174</article-id><article-id pub-id-type="publisher-id">PONE-D-16-14978</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cellular Structures and Organelles</subject><subj-group><subject>Cell Membranes</subject><subj-group><subject>Membrane Proteins</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cellular Structures and Organelles</subject><subj-group><subject>Cell Membranes</subject><subj-group><subject>Membrane Proteins</subject><subj-group><subject>Integral Membrane Proteins</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Parasitology</subject><subj-group><subject>Parasite Groups</subject><subj-group><subject>Apicomplexa</subject><subj-group><subject>Plasmodium</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cellular Structures and Organelles</subject><subj-group><subject>Endoplasmic Reticulum</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cell Processes</subject><subj-group><subject>Secretory Pathway</subject><subj-group><subject>Endoplasmic Reticulum</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cellular Structures and Organelles</subject><subj-group><subject>Vesicles</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and life sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteins</subject><subj-group><subject>DNA-binding proteins</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Molecular Biology</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Cloning</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Cloning</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Protozoans</subject><subj-group><subject>Parasitic Protozoans</subject><subj-group><subject>Malarial Parasites</subject></subj-group></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title><italic>Plasmodium falciparum</italic> Rab1A Localizes to Rhoptries in Schizonts</article-title><alt-title alt-title-type="running-head">PfRab1A and Rhoptry Protein Targeting</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Morse</surname><given-names>David</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="author-notes" rid="currentaff001"><sup>¤a</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib><contrib contrib-type="author"><name><surname>Webster</surname><given-names>Wesley</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="author-notes" rid="currentaff002"><sup>¤b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kalanon</surname><given-names>Ming</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="author-notes" rid="currentaff003"><sup>¤c</sup></xref></contrib><contrib contrib-type="author"><name><surname>Langsley</surname><given-names>Gordon</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>McFadden</surname><given-names>Geoffrey I.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, 75014, Paris, France</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Tsuboi</surname><given-names>Takafumi</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Ehime University, JAPAN</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: DM, GL, GM. Performed the experiments: DM, WW. Analyzed the data: DM, MK, GL, GM. Contributed reagents/materials/analysis tools: DM, GL, GM. Wrote the paper: DM, GL.</p></fn><fn fn-type="current-aff" id="currentaff001"><label>¤a</label><p>Current address: On leave from the Institut de recherche en biologie végétale, Département de Sciences biologiques, Université de Montréal, Montréal, Canada</p></fn><fn fn-type="current-aff" id="currentaff002"><label>¤b</label><p>Current address: Centre for Regional and Rural Futures, Deakin University, Burwood, Australia</p></fn><fn fn-type="current-aff" id="currentaff003"><label>¤c</label><p>Current address: Faculty of Law, Deakin University, Burwood, Australia</p></fn><corresp id="cor001">* E-mail: <email>david.morse@umontreal.ca</email></corresp></author-notes><pub-date pub-type="epub"><day>27</day><month>6</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>11</volume><issue>6</issue><elocation-id>e0158174</elocation-id><history><date date-type="received"><day>13</day><month>4</month><year>2016</year></date><date date-type="accepted"><day>11</day><month>6</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 Morse et al</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Morse et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="pone.0158174.pdf"></self-uri><abstract><p>Over-expression of a GFP-<italic>Pf</italic>Rab1A fusion protein in <italic>Plasmodium falciparum</italic> schizonts produces a punctate pattern of fluorescence typical of rhoptries, secretory organelles involved in host cell invasion. The GFP-positive bodies were purified by a combination of differential and density gradient centrifugation and their protein content determined by MS/MS sequencing. Consistent with the GFP rhoptry-like pattern of transgenic parasites, four of the 19 proteins identified have been previously described to be rhoptry-associated and another four are ER or ER-associated proteins. Confirmation that GFP-<italic>Pf</italic>Rab1A decorates rhoptries was obtained by its co-localization with Rap1 and Ron4 in late phase schizonts. We conclude that <italic>Pf</italic>Rab1A potentially regulates vesicular traffic from the endoplasmic reticulum to the rhoptries in <italic>Apicomplexa</italic> parasites.</p></abstract><funding-group><award-group id="award001"><funding-source><institution-wrap><institution-id institution-id-type="funder-id">http://dx.doi.org/10.13039/100000025</institution-id><institution>National Institute of Mental Health</institution></institution-wrap></funding-source><principal-award-recipient>Geoff I. McFadden</principal-award-recipient></award-group><award-group id="award002"><funding-source><institution>Howard Hughes Medical Institute (US)</institution></funding-source><principal-award-recipient>Geoff I. McFadden</principal-award-recipient></award-group><award-group id="award003"><funding-source><institution>Labex ParaFrap</institution></funding-source><award-id>ANR-11-LABX-0024</award-id><principal-award-recipient><name><surname>Langsley</surname><given-names>Gordon</given-names></name></principal-award-recipient></award-group><funding-statement>Funded by National Health and Medical Research Council (Australia); Australian Research Council; Labex ParaFrap - ANR-11-LABX-0024. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><fig-count count="3"></fig-count><table-count count="1"></table-count><page-count count="13"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper and its Supporting Information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper and its Supporting Information files.</p></notes></front></pmc><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Victoria (État)</li><li>Île-de-France</li></region><settlement><li>Melbourne</li><li>Paris</li></settlement><orgName><li>Université Paris-Descartes</li><li>Université de Melbourne</li></orgName></list><tree><country name="Australie"><region name="Victoria (État)"><name sortKey="Morse, David" sort="Morse, David" uniqKey="Morse D" first="David" last="Morse">David Morse</name></region><name sortKey="Kalanon, Ming" sort="Kalanon, Ming" uniqKey="Kalanon M" first="Ming" last="Kalanon">Ming Kalanon</name><name sortKey="Mcfadden, Geoffrey I" sort="Mcfadden, Geoffrey I" uniqKey="Mcfadden G" first="Geoffrey I." last="Mcfadden">Geoffrey I. Mcfadden</name><name sortKey="Webster, Wesley" sort="Webster, Wesley" uniqKey="Webster W" first="Wesley" last="Webster">Wesley Webster</name></country><country name="France"><region name="Île-de-France"><name sortKey="Langsley, Gordon" sort="Langsley, Gordon" uniqKey="Langsley G" first="Gordon" last="Langsley">Gordon Langsley</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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