Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract
Identifieur interne : 003A94 ( PascalFrancis/Corpus ); précédent : 003A93; suivant : 003A95Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract
Auteurs : Glen C. Ulett ; Jaione Valle ; Christophe Beloin ; Orla Sherlock ; Jean-Marc Ghigo ; Mark A. SchembriSource :
- Infection and immunity [ 0019-9567 ] ; 2007.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Escherichia coli is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with the virulence of uropathogenic E. coli (UPEC) are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter subgroup of proteins. The best characterized of these proteins, antigen 43 (Ag43), is a self-recognizing adhesin that is associated with cell aggregation and biofilm formation in E. coli K-12. The sequenced genome of prototype UPEC strain CFT073 contains two variant Ag43-encoding genes located on pathogenicity islands. The biological significance of both of these genes and their role in UPEC pathogenesis have not been investigated previously. Here we performed a detailed molecular characterization analysis of Ag43a (c3655) and Ag43b (c1273) from UPEC CFT073. Expression of Ag43a and Ag43b in a K-12 background revealed that they possess different functional properties. Ag43a produced a strong aggregation phenotype and promoted significant biofilm growth. Deletion mutants and strains constitutively expressing Ag43a and Ag43b were also constructed using CFT073. When these mutants were analyzed in a mouse model of UTI, Ag43a (but not Ag43b) promoted long-term persistence in the urinary bladder. Our findings demonstrate that Ag43a contributes to UPEC disease pathogenesis and reveal that there are pathogenicity-adapted variants of Ag43 with distinct virulence-related functions.
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| NO : | PASCAL 07-0361242 INIST |
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| ET : | Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract |
| AU : | ULETT (Glen C.); VALLE (Jaione); BELOIN (Christophe); SHERLOCK (Orla); GHIGO (Jean-Marc); SCHEMBRI (Mark A.) |
| AF : | School of Molecular and Microbial Sciences, University of Queensland/Brisbane, QLD 4072/Australie (1 aut., 4 aut., 6 aut.); Groupe de genetique des biofilms, URA CNRS 2172, Institut Pasteur, 25, rue du Dr Roux/75724 Paris/France (2 aut., 3 aut., 5 aut.) |
| DT : | Publication en série; Niveau analytique |
| SO : | Infection and immunity; ISSN 0019-9567; Coden INFIBR; Etats-Unis; Da. 2007; Vol. 75; No. 7; Pp. 3233-3244; Bibl. 57 ref. |
| LA : | Anglais |
| EA : | Escherichia coli is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with the virulence of uropathogenic E. coli (UPEC) are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter subgroup of proteins. The best characterized of these proteins, antigen 43 (Ag43), is a self-recognizing adhesin that is associated with cell aggregation and biofilm formation in E. coli K-12. The sequenced genome of prototype UPEC strain CFT073 contains two variant Ag43-encoding genes located on pathogenicity islands. The biological significance of both of these genes and their role in UPEC pathogenesis have not been investigated previously. Here we performed a detailed molecular characterization analysis of Ag43a (c3655) and Ag43b (c1273) from UPEC CFT073. Expression of Ag43a and Ag43b in a K-12 background revealed that they possess different functional properties. Ag43a produced a strong aggregation phenotype and promoted significant biofilm growth. Deletion mutants and strains constitutively expressing Ag43a and Ag43b were also constructed using CFT073. When these mutants were analyzed in a mouse model of UTI, Ag43a (but not Ag43b) promoted long-term persistence in the urinary bladder. Our findings demonstrate that Ag43a contributes to UPEC disease pathogenesis and reveal that there are pathogenicity-adapted variants of Ag43 with distinct virulence-related functions. |
| CC : | 002A05B15 |
| FD : | Escherichia coli; Antigène; Souche uropathogène |
| FG : | Enterobacteriaceae; Bactérie |
| ED : | Escherichia coli; Antigen |
| EG : | Enterobacteriaceae; Bacteria |
| SD : | Escherichia coli; Antígeno |
| LO : | INIST-15757.354000162965770030 |
| ID : | 07-0361242 |
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Ulett</name><affiliation><inist:fA14 i1="01"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1><s2>Brisbane, QLD 4072</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Valle, Jaione" sort="Valle, Jaione" uniqKey="Valle J" first="Jaione" last="Valle">Jaione Valle</name><affiliation><inist:fA14 i1="02"><s1>Groupe de genetique des biofilms, URA CNRS 2172, Institut Pasteur, 25, rue du Dr Roux</s1><s2>75724 Paris</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Beloin, Christophe" sort="Beloin, Christophe" uniqKey="Beloin C" first="Christophe" last="Beloin">Christophe Beloin</name><affiliation><inist:fA14 i1="02"><s1>Groupe de genetique des biofilms, URA CNRS 2172, Institut Pasteur, 25, rue du Dr Roux</s1><s2>75724 Paris</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Sherlock, Orla" sort="Sherlock, Orla" uniqKey="Sherlock O" first="Orla" last="Sherlock">Orla Sherlock</name><affiliation><inist:fA14 i1="01"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1><s2>Brisbane, QLD 4072</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Ghigo, Jean Marc" sort="Ghigo, Jean Marc" uniqKey="Ghigo J" first="Jean-Marc" last="Ghigo">Jean-Marc Ghigo</name><affiliation><inist:fA14 i1="02"><s1>Groupe de genetique des biofilms, URA CNRS 2172, Institut Pasteur, 25, rue du Dr Roux</s1><s2>75724 Paris</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Schembri, Mark A" sort="Schembri, Mark A" uniqKey="Schembri M" first="Mark A." last="Schembri">Mark A. Schembri</name><affiliation><inist:fA14 i1="01"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1><s2>Brisbane, QLD 4072</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14></affiliation></author></analytic><series><title level="j" type="main">Infection and immunity</title><title level="j" type="abbreviated">Infect. immun.</title><idno type="ISSN">0019-9567</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Infection and immunity</title><title level="j" type="abbreviated">Infect. immun.</title><idno type="ISSN">0019-9567</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigen</term><term>Escherichia coli</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Escherichia coli</term><term>Antigène</term><term>Souche uropathogène</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Escherichia coli is the primary cause of urinary tract infection (UTI) in the developed world. 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Ag43a produced a strong aggregation phenotype and promoted significant biofilm growth. Deletion mutants and strains constitutively expressing Ag43a and Ag43b were also constructed using CFT073. When these mutants were analyzed in a mouse model of UTI, Ag43a (but not Ag43b) promoted long-term persistence in the urinary bladder. Our findings demonstrate that Ag43a contributes to UPEC disease pathogenesis and reveal that there are pathogenicity-adapted variants of Ag43 with distinct virulence-related functions.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0019-9567</s0></fA01><fA02 i1="01"><s0>INFIBR</s0></fA02><fA03 i2="1"><s0>Infect. immun.</s0></fA03><fA05><s2>75</s2></fA05><fA06><s2>7</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract</s1></fA08><fA11 i1="01" i2="1"><s1>ULETT (Glen C.)</s1></fA11><fA11 i1="02" i2="1"><s1>VALLE (Jaione)</s1></fA11><fA11 i1="03" i2="1"><s1>BELOIN (Christophe)</s1></fA11><fA11 i1="04" i2="1"><s1>SHERLOCK (Orla)</s1></fA11><fA11 i1="05" i2="1"><s1>GHIGO (Jean-Marc)</s1></fA11><fA11 i1="06" i2="1"><s1>SCHEMBRI (Mark A.)</s1></fA11><fA14 i1="01"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1><s2>Brisbane, QLD 4072</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></fA14><fA14 i1="02"><s1>Groupe de genetique des biofilms, URA CNRS 2172, Institut Pasteur, 25, rue du Dr Roux</s1><s2>75724 Paris</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ></fA14><fA20><s1>3233-3244</s1></fA20><fA21><s1>2007</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>15757</s2><s5>354000162965770030</s5></fA43><fA44><s0>0000</s0><s1>© 2007 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>57 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>07-0361242</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Infection and immunity</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>Escherichia coli is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with the virulence of uropathogenic E. coli (UPEC) are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter subgroup of proteins. The best characterized of these proteins, antigen 43 (Ag43), is a self-recognizing adhesin that is associated with cell aggregation and biofilm formation in E. coli K-12. The sequenced genome of prototype UPEC strain CFT073 contains two variant Ag43-encoding genes located on pathogenicity islands. The biological significance of both of these genes and their role in UPEC pathogenesis have not been investigated previously. Here we performed a detailed molecular characterization analysis of Ag43a (c3655) and Ag43b (c1273) from UPEC CFT073. Expression of Ag43a and Ag43b in a K-12 background revealed that they possess different functional properties. Ag43a produced a strong aggregation phenotype and promoted significant biofilm growth. Deletion mutants and strains constitutively expressing Ag43a and Ag43b were also constructed using CFT073. When these mutants were analyzed in a mouse model of UTI, Ag43a (but not Ag43b) promoted long-term persistence in the urinary bladder. 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The major factors associated with the virulence of uropathogenic E. coli (UPEC) are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter subgroup of proteins. The best characterized of these proteins, antigen 43 (Ag43), is a self-recognizing adhesin that is associated with cell aggregation and biofilm formation in E. coli K-12. The sequenced genome of prototype UPEC strain CFT073 contains two variant Ag43-encoding genes located on pathogenicity islands. The biological significance of both of these genes and their role in UPEC pathogenesis have not been investigated previously. Here we performed a detailed molecular characterization analysis of Ag43a (c3655) and Ag43b (c1273) from UPEC CFT073. Expression of Ag43a and Ag43b in a K-12 background revealed that they possess different functional properties. Ag43a produced a strong aggregation phenotype and promoted significant biofilm growth. Deletion mutants and strains constitutively expressing Ag43a and Ag43b were also constructed using CFT073. When these mutants were analyzed in a mouse model of UTI, Ag43a (but not Ag43b) promoted long-term persistence in the urinary bladder. Our findings demonstrate that Ag43a contributes to UPEC disease pathogenesis and reveal that there are pathogenicity-adapted variants of Ag43 with distinct virulence-related functions.</EA><CC>002A05B15</CC><FD>Escherichia coli; Antigène; Souche uropathogène</FD><FG>Enterobacteriaceae; Bactérie</FG><ED>Escherichia coli; Antigen</ED><EG>Enterobacteriaceae; Bacteria</EG><SD>Escherichia coli; Antígeno</SD><LO>INIST-15757.354000162965770030</LO><ID>07-0361242</ID></server></inist></record>Pour manipuler ce document sous Unix (Dilib)
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