CRISPR/Cas9 in the Chicken Embryo.
Identifieur interne : 004C95 ( Ncbi/Curation ); précédent : 004C94; suivant : 004C96CRISPR/Cas9 in the Chicken Embryo.
Auteurs : Valérie Morin [France] ; Nadège Véron [Australie] ; Christophe Marcelle [France]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2017.
Abstract
Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms. We recently showed that this technique can be combined with in vivo electroporation to inhibit the function of genes of interest in somatic cells of the developing chicken embryo. We present here a simplified version of the previously described technique that leads to effective gene loss-of-function.
DOI: 10.1007/978-1-4939-7216-6_7
PubMed: 28809017
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pubmed:28809017Le document en format XML
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<front><div type="abstract" xml:lang="en">Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms. We recently showed that this technique can be combined with in vivo electroporation to inhibit the function of genes of interest in somatic cells of the developing chicken embryo. We present here a simplified version of the previously described technique that leads to effective gene loss-of-function.</div>
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