AustralieFrV1, PubMed, Corpus, bibRecord, 001191

CRISPR/Cas9 in the Chicken Embryo.

Identifieur interne : 001191 ( PubMed/Corpus ); précédent : 001190; suivant : 001192

CRISPR/Cas9 in the Chicken Embryo.

Auteurs : Valérie Morin ; Nadège Véron ; Christophe Marcelle

Source :

Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2017.

RBID : pubmed:28809017

Abstract

Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms. We recently showed that this technique can be combined with in vivo electroporation to inhibit the function of genes of interest in somatic cells of the developing chicken embryo. We present here a simplified version of the previously described technique that leads to effective gene loss-of-function.

DOI: 10.1007/978-1-4939-7216-6_7
PubMed: 28809017

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pubmed:28809017

Le document en format XML

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<author><name sortKey="Morin, Valerie" sort="Morin, Valerie" uniqKey="Morin V" first="Valérie" last="Morin">Valérie Morin</name>
<affiliation><nlm:affiliation>Institut NeuroMyoGène, INMG, Faculty of Medicine Laënnec, University Lyon1, Bâtiment B, 7 rue Guillaume Paradin, 69008, Lyon, France.</nlm:affiliation>
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<author><name sortKey="Veron, Nadege" sort="Veron, Nadege" uniqKey="Veron N" first="Nadège" last="Véron">Nadège Véron</name>
<affiliation><nlm:affiliation>EMBL Australia, Australian Regenerative Medicine Institute, Monash University, 15 Innovation Walk, Clayton, VIC, 3800, Australia.</nlm:affiliation>
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<author><name sortKey="Marcelle, Christophe" sort="Marcelle, Christophe" uniqKey="Marcelle C" first="Christophe" last="Marcelle">Christophe Marcelle</name>
<affiliation><nlm:affiliation>Institut NeuroMyoGène, INMG, Faculty of Medicine Laënnec, University Lyon1, Bâtiment B, 7 rue Guillaume Paradin, 69008, Lyon, France. christophe.marcelle@monash.edu.</nlm:affiliation>
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<front><div type="abstract" xml:lang="en">Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms. We recently showed that this technique can be combined with in vivo electroporation to inhibit the function of genes of interest in somatic cells of the developing chicken embryo. We present here a simplified version of the previously described technique that leads to effective gene loss-of-function.</div>
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<Abstract><AbstractText>Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms. We recently showed that this technique can be combined with in vivo electroporation to inhibit the function of genes of interest in somatic cells of the developing chicken embryo. We present here a simplified version of the previously described technique that leads to effective gene loss-of-function.</AbstractText>
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CRISPR/Cas9 in the Chicken Embryo.

CRISPR/Cas9 in the Chicken Embryo.

Source :

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki