A simple way to track single gold-loaded alginate microcapsules using x-ray CT in small animal longitudinal studies.
Identifieur interne : 001B57 ( Ncbi/Curation ); précédent : 001B56; suivant : 001B58A simple way to track single gold-loaded alginate microcapsules using x-ray CT in small animal longitudinal studies.
Auteurs : Alberto Astolfo [Australie] ; Fengxiang Qie [Australie] ; Astrid Kibleur [France] ; Xiaojuan Hao [Australie] ; Ralf Hendrik Menk [Italie] ; Fulvia Arfelli [Italie] ; Luigi Rigon [Italie] ; Tracey M. Hinton [Australie] ; Malsha Wickramaratna [Australie] ; Tianwei Tan [République populaire de Chine] ; Timothy C. Hughes [Australie]Source :
- Nanomedicine : nanotechnology, biology, and medicine [ 1549-9642 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Alginates, Capsules, Glucuronic Acid, Gold, Hexuronic Acids.
- chemistry : Metal Nanoparticles.
- Animals, Tomography, X-Ray Computed.
Abstract
The use of alginate based microcapsules to deliver drugs and cells with a minimal host interaction is increasingly being proposed. A proficient method to track the position of the microcapsules during such therapies, particularly if they are amenable to commonly used instrumentation, would greatly help the development of such treatments. Here we propose to label the microcapsules with gold nanoparticles to provide a bright contrast on small animal x-ray micro-CT systems enabling single microcapsule detection. The microcapsules preparation is based on a simple protocol using inexpensive compounds. This, combined with the widespread availability of micro-CT apparatus, renders our method more accessible compared with other methods. Our labeled microcapsules showed good mechanical stability and low cytotoxicity in-vitro. Our post-mortem rodent model data strongly suggest that the high signal intensity generated by the labeled microcapsules permits the use of a reduced radiation dose yielding a method fully compatible with longitudinal in-vivo studies.
DOI: 10.1016/j.nano.2014.06.008
PubMed: 24954384
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :003359
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :003248
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :003248
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001B57
Links to Exploration step
pubmed:24954384Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A simple way to track single gold-loaded alginate microcapsules using x-ray CT in small animal longitudinal studies.</title>
<author><name sortKey="Astolfo, Alberto" sort="Astolfo, Alberto" uniqKey="Astolfo A" first="Alberto" last="Astolfo">Alberto Astolfo</name>
<affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, 5232 Villigen, PSI, Switzerland; Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria, Australia. Electronic address: alberto.astolfo@psi.ch.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Swiss Light Source, 5232 Villigen, PSI, Switzerland; Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Qie, Fengxiang" sort="Qie, Fengxiang" uniqKey="Qie F" first="Fengxiang" last="Qie">Fengxiang Qie</name>
<affiliation wicri:level="1"><nlm:affiliation>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China; CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China; CSIRO Materials Science and Engineering, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kibleur, Astrid" sort="Kibleur, Astrid" uniqKey="Kibleur A" first="Astrid" last="Kibleur">Astrid Kibleur</name>
<affiliation wicri:level="3"><nlm:affiliation>Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria, Australia; Grenoble Institut des Neurosciences, Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria, Australia; Grenoble Institut des Neurosciences, Grenoble</wicri:regionArea>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hao, Xiaojuan" sort="Hao, Xiaojuan" uniqKey="Hao X" first="Xiaojuan" last="Hao">Xiaojuan Hao</name>
<affiliation wicri:level="1"><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>CSIRO Materials Science and Engineering, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Menk, Ralf Hendrik" sort="Menk, Ralf Hendrik" uniqKey="Menk R" first="Ralf Hendrik" last="Menk">Ralf Hendrik Menk</name>
<affiliation wicri:level="1"><nlm:affiliation>Elettra-Sincrotrone Trieste S.C.p.A., Trieste, Italy; Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Elettra-Sincrotrone Trieste S.C.p.A., Trieste, Italy; Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste</wicri:regionArea>
<wicri:noRegion>Trieste</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Arfelli, Fulvia" sort="Arfelli, Fulvia" uniqKey="Arfelli F" first="Fulvia" last="Arfelli">Fulvia Arfelli</name>
<affiliation wicri:level="1"><nlm:affiliation>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste</wicri:regionArea>
<wicri:noRegion>Trieste</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rigon, Luigi" sort="Rigon, Luigi" uniqKey="Rigon L" first="Luigi" last="Rigon">Luigi Rigon</name>
<affiliation wicri:level="1"><nlm:affiliation>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste</wicri:regionArea>
<wicri:noRegion>Trieste</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hinton, Tracey M" sort="Hinton, Tracey M" uniqKey="Hinton T" first="Tracey M" last="Hinton">Tracey M. Hinton</name>
<affiliation wicri:level="1"><nlm:affiliation>CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>CSIRO Animal, Food and Health Sciences, Geelong, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wickramaratna, Malsha" sort="Wickramaratna, Malsha" uniqKey="Wickramaratna M" first="Malsha" last="Wickramaratna">Malsha Wickramaratna</name>
<affiliation wicri:level="1"><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>CSIRO Materials Science and Engineering, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tan, Tianwei" sort="Tan, Tianwei" uniqKey="Tan T" first="Tianwei" last="Tan">Tianwei Tan</name>
<affiliation wicri:level="3"><nlm:affiliation>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hughes, Timothy C" sort="Hughes, Timothy C" uniqKey="Hughes T" first="Timothy C" last="Hughes">Timothy C. Hughes</name>
<affiliation wicri:level="1"><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia. Electronic address: tim.hughes@csiro.au.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>CSIRO Materials Science and Engineering, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2014">2014</date>
<idno type="RBID">pubmed:24954384</idno>
<idno type="pmid">24954384</idno>
<idno type="doi">10.1016/j.nano.2014.06.008</idno>
<idno type="wicri:Area/PubMed/Corpus">003359</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003359</idno>
<idno type="wicri:Area/PubMed/Curation">003248</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003248</idno>
<idno type="wicri:Area/PubMed/Checkpoint">003248</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003248</idno>
<idno type="wicri:Area/Ncbi/Merge">001B57</idno>
<idno type="wicri:Area/Ncbi/Curation">001B57</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A simple way to track single gold-loaded alginate microcapsules using x-ray CT in small animal longitudinal studies.</title>
<author><name sortKey="Astolfo, Alberto" sort="Astolfo, Alberto" uniqKey="Astolfo A" first="Alberto" last="Astolfo">Alberto Astolfo</name>
<affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, 5232 Villigen, PSI, Switzerland; Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria, Australia. Electronic address: alberto.astolfo@psi.ch.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Swiss Light Source, 5232 Villigen, PSI, Switzerland; Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Qie, Fengxiang" sort="Qie, Fengxiang" uniqKey="Qie F" first="Fengxiang" last="Qie">Fengxiang Qie</name>
<affiliation wicri:level="1"><nlm:affiliation>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China; CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China; CSIRO Materials Science and Engineering, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kibleur, Astrid" sort="Kibleur, Astrid" uniqKey="Kibleur A" first="Astrid" last="Kibleur">Astrid Kibleur</name>
<affiliation wicri:level="3"><nlm:affiliation>Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria, Australia; Grenoble Institut des Neurosciences, Grenoble, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria, Australia; Grenoble Institut des Neurosciences, Grenoble</wicri:regionArea>
<placeName><region type="region">Auvergne-Rhône-Alpes</region>
<region type="old region">Rhône-Alpes</region>
<settlement type="city">Grenoble</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hao, Xiaojuan" sort="Hao, Xiaojuan" uniqKey="Hao X" first="Xiaojuan" last="Hao">Xiaojuan Hao</name>
<affiliation wicri:level="1"><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>CSIRO Materials Science and Engineering, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Menk, Ralf Hendrik" sort="Menk, Ralf Hendrik" uniqKey="Menk R" first="Ralf Hendrik" last="Menk">Ralf Hendrik Menk</name>
<affiliation wicri:level="1"><nlm:affiliation>Elettra-Sincrotrone Trieste S.C.p.A., Trieste, Italy; Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Elettra-Sincrotrone Trieste S.C.p.A., Trieste, Italy; Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste</wicri:regionArea>
<wicri:noRegion>Trieste</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Arfelli, Fulvia" sort="Arfelli, Fulvia" uniqKey="Arfelli F" first="Fulvia" last="Arfelli">Fulvia Arfelli</name>
<affiliation wicri:level="1"><nlm:affiliation>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste</wicri:regionArea>
<wicri:noRegion>Trieste</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rigon, Luigi" sort="Rigon, Luigi" uniqKey="Rigon L" first="Luigi" last="Rigon">Luigi Rigon</name>
<affiliation wicri:level="1"><nlm:affiliation>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste</wicri:regionArea>
<wicri:noRegion>Trieste</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hinton, Tracey M" sort="Hinton, Tracey M" uniqKey="Hinton T" first="Tracey M" last="Hinton">Tracey M. Hinton</name>
<affiliation wicri:level="1"><nlm:affiliation>CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>CSIRO Animal, Food and Health Sciences, Geelong, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wickramaratna, Malsha" sort="Wickramaratna, Malsha" uniqKey="Wickramaratna M" first="Malsha" last="Wickramaratna">Malsha Wickramaratna</name>
<affiliation wicri:level="1"><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>CSIRO Materials Science and Engineering, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tan, Tianwei" sort="Tan, Tianwei" uniqKey="Tan T" first="Tianwei" last="Tan">Tianwei Tan</name>
<affiliation wicri:level="3"><nlm:affiliation>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hughes, Timothy C" sort="Hughes, Timothy C" uniqKey="Hughes T" first="Timothy C" last="Hughes">Timothy C. Hughes</name>
<affiliation wicri:level="1"><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia. Electronic address: tim.hughes@csiro.au.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>CSIRO Materials Science and Engineering, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Nanomedicine : nanotechnology, biology, and medicine</title>
<idno type="eISSN">1549-9642</idno>
<imprint><date when="2014" type="published">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alginates (chemistry)</term>
<term>Animals</term>
<term>Capsules (chemistry)</term>
<term>Glucuronic Acid (chemistry)</term>
<term>Gold (chemistry)</term>
<term>Hexuronic Acids (chemistry)</term>
<term>Metal Nanoparticles (chemistry)</term>
<term>Tomography, X-Ray Computed</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acide glucuronique ()</term>
<term>Acides hexuroniques ()</term>
<term>Alginates ()</term>
<term>Animaux</term>
<term>Capsules ()</term>
<term>Nanoparticules métalliques ()</term>
<term>Or ()</term>
<term>Tomodensitométrie</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Alginates</term>
<term>Capsules</term>
<term>Glucuronic Acid</term>
<term>Gold</term>
<term>Hexuronic Acids</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Tomography, X-Ray Computed</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Acide glucuronique</term>
<term>Acides hexuroniques</term>
<term>Alginates</term>
<term>Animaux</term>
<term>Capsules</term>
<term>Nanoparticules métalliques</term>
<term>Or</term>
<term>Tomodensitométrie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The use of alginate based microcapsules to deliver drugs and cells with a minimal host interaction is increasingly being proposed. A proficient method to track the position of the microcapsules during such therapies, particularly if they are amenable to commonly used instrumentation, would greatly help the development of such treatments. Here we propose to label the microcapsules with gold nanoparticles to provide a bright contrast on small animal x-ray micro-CT systems enabling single microcapsule detection. The microcapsules preparation is based on a simple protocol using inexpensive compounds. This, combined with the widespread availability of micro-CT apparatus, renders our method more accessible compared with other methods. Our labeled microcapsules showed good mechanical stability and low cytotoxicity in-vitro. Our post-mortem rodent model data strongly suggest that the high signal intensity generated by the labeled microcapsules permits the use of a reduced radiation dose yielding a method fully compatible with longitudinal in-vivo studies.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001B57 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 001B57 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Ncbi |étape= Curation |type= RBID |clé= pubmed:24954384 |texte= A simple way to track single gold-loaded alginate microcapsules using x-ray CT in small animal longitudinal studies. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:24954384" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a AustralieFrV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |