A simple way to track single gold-loaded alginate microcapsules using x-ray CT in small animal longitudinal studies.
Identifieur interne : 003359 ( PubMed/Corpus ); précédent : 003358; suivant : 003360A simple way to track single gold-loaded alginate microcapsules using x-ray CT in small animal longitudinal studies.
Auteurs : Alberto Astolfo ; Fengxiang Qie ; Astrid Kibleur ; Xiaojuan Hao ; Ralf Hendrik Menk ; Fulvia Arfelli ; Luigi Rigon ; Tracey M. Hinton ; Malsha Wickramaratna ; Tianwei Tan ; Timothy C. HughesSource :
- Nanomedicine : nanotechnology, biology, and medicine [ 1549-9642 ] ; 2014.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Alginates, Capsules, Glucuronic Acid, Gold, Hexuronic Acids.
- chemistry : Metal Nanoparticles.
- Animals, Tomography, X-Ray Computed.
Abstract
The use of alginate based microcapsules to deliver drugs and cells with a minimal host interaction is increasingly being proposed. A proficient method to track the position of the microcapsules during such therapies, particularly if they are amenable to commonly used instrumentation, would greatly help the development of such treatments. Here we propose to label the microcapsules with gold nanoparticles to provide a bright contrast on small animal x-ray micro-CT systems enabling single microcapsule detection. The microcapsules preparation is based on a simple protocol using inexpensive compounds. This, combined with the widespread availability of micro-CT apparatus, renders our method more accessible compared with other methods. Our labeled microcapsules showed good mechanical stability and low cytotoxicity in-vitro. Our post-mortem rodent model data strongly suggest that the high signal intensity generated by the labeled microcapsules permits the use of a reduced radiation dose yielding a method fully compatible with longitudinal in-vivo studies.
DOI: 10.1016/j.nano.2014.06.008
PubMed: 24954384
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pubmed:24954384Le document en format XML
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Electronic address: alberto.astolfo@psi.ch.</nlm:affiliation></affiliation></author><author><name sortKey="Qie, Fengxiang" sort="Qie, Fengxiang" uniqKey="Qie F" first="Fengxiang" last="Qie">Fengxiang Qie</name><affiliation><nlm:affiliation>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China; CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Kibleur, Astrid" sort="Kibleur, Astrid" uniqKey="Kibleur A" first="Astrid" last="Kibleur">Astrid Kibleur</name><affiliation><nlm:affiliation>Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria, Australia; Grenoble Institut des Neurosciences, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Hao, Xiaojuan" sort="Hao, Xiaojuan" uniqKey="Hao X" first="Xiaojuan" last="Hao">Xiaojuan Hao</name><affiliation><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Menk, Ralf Hendrik" sort="Menk, Ralf Hendrik" uniqKey="Menk R" first="Ralf Hendrik" last="Menk">Ralf Hendrik Menk</name><affiliation><nlm:affiliation>Elettra-Sincrotrone Trieste S.C.p.A., Trieste, Italy; Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Arfelli, Fulvia" sort="Arfelli, Fulvia" uniqKey="Arfelli F" first="Fulvia" last="Arfelli">Fulvia Arfelli</name><affiliation><nlm:affiliation>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Rigon, Luigi" sort="Rigon, Luigi" uniqKey="Rigon L" first="Luigi" last="Rigon">Luigi Rigon</name><affiliation><nlm:affiliation>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Hinton, Tracey M" sort="Hinton, Tracey M" uniqKey="Hinton T" first="Tracey M" last="Hinton">Tracey M. Hinton</name><affiliation><nlm:affiliation>CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Wickramaratna, Malsha" sort="Wickramaratna, Malsha" uniqKey="Wickramaratna M" first="Malsha" last="Wickramaratna">Malsha Wickramaratna</name><affiliation><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Tan, Tianwei" sort="Tan, Tianwei" uniqKey="Tan T" first="Tianwei" last="Tan">Tianwei Tan</name><affiliation><nlm:affiliation>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Hughes, Timothy C" sort="Hughes, Timothy C" uniqKey="Hughes T" first="Timothy C" last="Hughes">Timothy C. Hughes</name><affiliation><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia. Electronic address: tim.hughes@csiro.au.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24954384</idno><idno type="pmid">24954384</idno><idno type="doi">10.1016/j.nano.2014.06.008</idno><idno type="wicri:Area/PubMed/Corpus">003359</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003359</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A simple way to track single gold-loaded alginate microcapsules using x-ray CT in small animal longitudinal studies.</title><author><name sortKey="Astolfo, Alberto" sort="Astolfo, Alberto" uniqKey="Astolfo A" first="Alberto" last="Astolfo">Alberto Astolfo</name><affiliation><nlm:affiliation>Swiss Light Source, 5232 Villigen, PSI, Switzerland; Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria, Australia. Electronic address: alberto.astolfo@psi.ch.</nlm:affiliation></affiliation></author><author><name sortKey="Qie, Fengxiang" sort="Qie, Fengxiang" uniqKey="Qie F" first="Fengxiang" last="Qie">Fengxiang Qie</name><affiliation><nlm:affiliation>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China; CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Kibleur, Astrid" sort="Kibleur, Astrid" uniqKey="Kibleur A" first="Astrid" last="Kibleur">Astrid Kibleur</name><affiliation><nlm:affiliation>Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria, Australia; Grenoble Institut des Neurosciences, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Hao, Xiaojuan" sort="Hao, Xiaojuan" uniqKey="Hao X" first="Xiaojuan" last="Hao">Xiaojuan Hao</name><affiliation><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Menk, Ralf Hendrik" sort="Menk, Ralf Hendrik" uniqKey="Menk R" first="Ralf Hendrik" last="Menk">Ralf Hendrik Menk</name><affiliation><nlm:affiliation>Elettra-Sincrotrone Trieste S.C.p.A., Trieste, Italy; Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Arfelli, Fulvia" sort="Arfelli, Fulvia" uniqKey="Arfelli F" first="Fulvia" last="Arfelli">Fulvia Arfelli</name><affiliation><nlm:affiliation>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Rigon, Luigi" sort="Rigon, Luigi" uniqKey="Rigon L" first="Luigi" last="Rigon">Luigi Rigon</name><affiliation><nlm:affiliation>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Hinton, Tracey M" sort="Hinton, Tracey M" uniqKey="Hinton T" first="Tracey M" last="Hinton">Tracey M. Hinton</name><affiliation><nlm:affiliation>CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Wickramaratna, Malsha" sort="Wickramaratna, Malsha" uniqKey="Wickramaratna M" first="Malsha" last="Wickramaratna">Malsha Wickramaratna</name><affiliation><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Tan, Tianwei" sort="Tan, Tianwei" uniqKey="Tan T" first="Tianwei" last="Tan">Tianwei Tan</name><affiliation><nlm:affiliation>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Hughes, Timothy C" sort="Hughes, Timothy C" uniqKey="Hughes T" first="Timothy C" last="Hughes">Timothy C. Hughes</name><affiliation><nlm:affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia. Electronic address: tim.hughes@csiro.au.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Nanomedicine : nanotechnology, biology, and medicine</title><idno type="eISSN">1549-9642</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alginates (chemistry)</term><term>Animals</term><term>Capsules (chemistry)</term><term>Glucuronic Acid (chemistry)</term><term>Gold (chemistry)</term><term>Hexuronic Acids (chemistry)</term><term>Metal Nanoparticles (chemistry)</term><term>Tomography, X-Ray Computed</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Alginates</term><term>Capsules</term><term>Glucuronic Acid</term><term>Gold</term><term>Hexuronic Acids</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Tomography, X-Ray Computed</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The use of alginate based microcapsules to deliver drugs and cells with a minimal host interaction is increasingly being proposed. A proficient method to track the position of the microcapsules during such therapies, particularly if they are amenable to commonly used instrumentation, would greatly help the development of such treatments. Here we propose to label the microcapsules with gold nanoparticles to provide a bright contrast on small animal x-ray micro-CT systems enabling single microcapsule detection. The microcapsules preparation is based on a simple protocol using inexpensive compounds. This, combined with the widespread availability of micro-CT apparatus, renders our method more accessible compared with other methods. Our labeled microcapsules showed good mechanical stability and low cytotoxicity in-vitro. Our post-mortem rodent model data strongly suggest that the high signal intensity generated by the labeled microcapsules permits the use of a reduced radiation dose yielding a method fully compatible with longitudinal in-vivo studies.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24954384</PMID><DateCreated><Year>2014</Year><Month>12</Month><Day>02</Day></DateCreated><DateCompleted><Year>2015</Year><Month>07</Month><Day>15</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1549-9642</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>8</Issue><PubDate><Year>2014</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Nanomedicine : nanotechnology, biology, and medicine</Title><ISOAbbreviation>Nanomedicine</ISOAbbreviation></Journal><ArticleTitle>A simple way to track single gold-loaded alginate microcapsules using x-ray CT in small animal longitudinal studies.</ArticleTitle><Pagination><MedlinePgn>1821-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.nano.2014.06.008</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1549-9634(14)00321-9</ELocationID><Abstract><AbstractText Label="UNLABELLED">The use of alginate based microcapsules to deliver drugs and cells with a minimal host interaction is increasingly being proposed. A proficient method to track the position of the microcapsules during such therapies, particularly if they are amenable to commonly used instrumentation, would greatly help the development of such treatments. Here we propose to label the microcapsules with gold nanoparticles to provide a bright contrast on small animal x-ray micro-CT systems enabling single microcapsule detection. The microcapsules preparation is based on a simple protocol using inexpensive compounds. This, combined with the widespread availability of micro-CT apparatus, renders our method more accessible compared with other methods. Our labeled microcapsules showed good mechanical stability and low cytotoxicity in-vitro. Our post-mortem rodent model data strongly suggest that the high signal intensity generated by the labeled microcapsules permits the use of a reduced radiation dose yielding a method fully compatible with longitudinal in-vivo studies.</AbstractText><AbstractText Label="FROM THE CLINICAL EDITOR" NlmCategory="UNASSIGNED">The authors of this study report the development of a micro-CT based tracking method of alginate-based microcapsules by incorporating gold nanoparticles in the microcapsules. They demonstrate the feasibility of this system in rodent models, where due to the high signal intensity, even reduced radiation dose is sufficient to track these particles, providing a simple and effective method to track these commonly used microcapsules and allowing longitudinal studies.</AbstractText><CopyrightInformation>Crown Copyright © 2014. Published by Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Astolfo</LastName><ForeName>Alberto</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Swiss Light Source, 5232 Villigen, PSI, Switzerland; Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria, Australia. Electronic address: alberto.astolfo@psi.ch.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qie</LastName><ForeName>Fengxiang</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China; CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kibleur</LastName><ForeName>Astrid</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Australian Synchrotron, Imaging and Medical Beam Line (IMBL), Clayton, Victoria, Australia; Grenoble Institut des Neurosciences, Grenoble, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hao</LastName><ForeName>Xiaojuan</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Menk</LastName><ForeName>Ralf Hendrik</ForeName><Initials>RH</Initials><AffiliationInfo><Affiliation>Elettra-Sincrotrone Trieste S.C.p.A., Trieste, Italy; Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arfelli</LastName><ForeName>Fulvia</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rigon</LastName><ForeName>Luigi</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Istituto Nazionale di Fisica Nucleare-Sezione di Trieste, Trieste, Italy; Department of Physics, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hinton</LastName><ForeName>Tracey M</ForeName><Initials>TM</Initials><AffiliationInfo><Affiliation>CSIRO Animal, Food and Health Sciences, Geelong, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wickramaratna</LastName><ForeName>Malsha</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Tianwei</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hughes</LastName><ForeName>Timothy C</ForeName><Initials>TC</Initials><AffiliationInfo><Affiliation>CSIRO Materials Science and Engineering, Clayton, Victoria, Australia. Electronic address: tim.hughes@csiro.au.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>06</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Nanomedicine</MedlineTA><NlmUniqueID>101233142</NlmUniqueID><ISSNLinking>1549-9634</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000464">Alginates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002214">Capsules</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006603">Hexuronic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-57-5</RegistryNumber><NameOfSubstance UI="D006046">Gold</NameOfSubstance></Chemical><Chemical><RegistryNumber>8A5D83Q4RW</RegistryNumber><NameOfSubstance UI="D020723">Glucuronic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>8C3Z4148WZ</RegistryNumber><NameOfSubstance UI="C008970">alginic acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000464" MajorTopicYN="N">Alginates</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002214" MajorTopicYN="N">Capsules</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020723" MajorTopicYN="N">Glucuronic Acid</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006046" MajorTopicYN="N">Gold</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006603" MajorTopicYN="N">Hexuronic Acids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053768" MajorTopicYN="N">Metal Nanoparticles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014057" MajorTopicYN="N">Tomography, X-Ray Computed</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Alginate microcapsules</Keyword><Keyword MajorTopicYN="N">Computed tomography</Keyword><Keyword MajorTopicYN="N">Gold nanoparticles</Keyword><Keyword MajorTopicYN="N">Reversible Addition–Fragmentation chain Transfer (RAFT) polymerization</Keyword><Keyword MajorTopicYN="N">X-ray imaging</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>05</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>06</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>6</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>6</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>7</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24954384</ArticleId><ArticleId IdType="pii">S1549-9634(14)00321-9</ArticleId><ArticleId IdType="doi">10.1016/j.nano.2014.06.008</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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