A simple way to track single gold-loaded alginate microcapsules using x-ray CT in small animal longitudinal studies.
Identifieur interne : 003359 ( PubMed/Corpus ); précédent : 003358; suivant : 003360A simple way to track single gold-loaded alginate microcapsules using x-ray CT in small animal longitudinal studies.
Auteurs : Alberto Astolfo ; Fengxiang Qie ; Astrid Kibleur ; Xiaojuan Hao ; Ralf Hendrik Menk ; Fulvia Arfelli ; Luigi Rigon ; Tracey M. Hinton ; Malsha Wickramaratna ; Tianwei Tan ; Timothy C. HughesSource :
- Nanomedicine : nanotechnology, biology, and medicine [ 1549-9642 ] ; 2014.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Alginates, Capsules, Glucuronic Acid, Gold, Hexuronic Acids.
- chemistry : Metal Nanoparticles.
- Animals, Tomography, X-Ray Computed.
Abstract
The use of alginate based microcapsules to deliver drugs and cells with a minimal host interaction is increasingly being proposed. A proficient method to track the position of the microcapsules during such therapies, particularly if they are amenable to commonly used instrumentation, would greatly help the development of such treatments. Here we propose to label the microcapsules with gold nanoparticles to provide a bright contrast on small animal x-ray micro-CT systems enabling single microcapsule detection. The microcapsules preparation is based on a simple protocol using inexpensive compounds. This, combined with the widespread availability of micro-CT apparatus, renders our method more accessible compared with other methods. Our labeled microcapsules showed good mechanical stability and low cytotoxicity in-vitro. Our post-mortem rodent model data strongly suggest that the high signal intensity generated by the labeled microcapsules permits the use of a reduced radiation dose yielding a method fully compatible with longitudinal in-vivo studies.
DOI: 10.1016/j.nano.2014.06.008
PubMed: 24954384
Links to Exploration step
pubmed:24954384Le document en format XML
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<front><div type="abstract" xml:lang="en">The use of alginate based microcapsules to deliver drugs and cells with a minimal host interaction is increasingly being proposed. A proficient method to track the position of the microcapsules during such therapies, particularly if they are amenable to commonly used instrumentation, would greatly help the development of such treatments. Here we propose to label the microcapsules with gold nanoparticles to provide a bright contrast on small animal x-ray micro-CT systems enabling single microcapsule detection. The microcapsules preparation is based on a simple protocol using inexpensive compounds. This, combined with the widespread availability of micro-CT apparatus, renders our method more accessible compared with other methods. Our labeled microcapsules showed good mechanical stability and low cytotoxicity in-vitro. Our post-mortem rodent model data strongly suggest that the high signal intensity generated by the labeled microcapsules permits the use of a reduced radiation dose yielding a method fully compatible with longitudinal in-vivo studies.</div>
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<Abstract><AbstractText Label="UNLABELLED">The use of alginate based microcapsules to deliver drugs and cells with a minimal host interaction is increasingly being proposed. A proficient method to track the position of the microcapsules during such therapies, particularly if they are amenable to commonly used instrumentation, would greatly help the development of such treatments. Here we propose to label the microcapsules with gold nanoparticles to provide a bright contrast on small animal x-ray micro-CT systems enabling single microcapsule detection. The microcapsules preparation is based on a simple protocol using inexpensive compounds. This, combined with the widespread availability of micro-CT apparatus, renders our method more accessible compared with other methods. Our labeled microcapsules showed good mechanical stability and low cytotoxicity in-vitro. Our post-mortem rodent model data strongly suggest that the high signal intensity generated by the labeled microcapsules permits the use of a reduced radiation dose yielding a method fully compatible with longitudinal in-vivo studies.</AbstractText>
<AbstractText Label="FROM THE CLINICAL EDITOR" NlmCategory="UNASSIGNED">The authors of this study report the development of a micro-CT based tracking method of alginate-based microcapsules by incorporating gold nanoparticles in the microcapsules. They demonstrate the feasibility of this system in rodent models, where due to the high signal intensity, even reduced radiation dose is sufficient to track these particles, providing a simple and effective method to track these commonly used microcapsules and allowing longitudinal studies.</AbstractText>
<CopyrightInformation>Crown Copyright © 2014. Published by Elsevier Inc. All rights reserved.</CopyrightInformation>
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<ForeName>Timothy C</ForeName>
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