Cellular Response to Linear and Branched Poly(acrylic acid).
Identifieur interne : 002922 ( Ncbi/Checkpoint ); précédent : 002921; suivant : 002923Cellular Response to Linear and Branched Poly(acrylic acid).
Auteurs : Elizabeth G. Whitty [Australie] ; Alison R. Maniego [Australie] ; Sharon A. Bentwitch [Australie] ; Yohann Guillaneuf [France] ; Mark R. Jones [Australie] ; Marianne Gaborieau [Australie] ; Patrice Castignolles [Australie]Source :
- Macromolecular bioscience [ 1616-5195 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Acrylic Resins, Blood Proteins.
- metabolism : Myoblasts, Skeletal.
- Animals, Cell Line, Tumor, Cell Survival, Mice.
Abstract
Poly(acrylic acid-co-sodium acrylate) (PNaA) is a pH-responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3-arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72 h MTT assay most PNaAs exhibited a IC50 between 7 and 14 mmol L(-1), showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3-arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.
DOI: 10.1002/mabi.201500153
PubMed: 26257305
Affiliations:
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Whitty</name><affiliation wicri:level="1"><nlm:affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751</wicri:regionArea><wicri:noRegion>2751</wicri:noRegion></affiliation></author><author><name sortKey="Maniego, Alison R" sort="Maniego, Alison R" uniqKey="Maniego A" first="Alison R" last="Maniego">Alison R. Maniego</name><affiliation wicri:level="1"><nlm:affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751</wicri:regionArea><wicri:noRegion>2751</wicri:noRegion></affiliation></author><author><name sortKey="Bentwitch, Sharon A" sort="Bentwitch, Sharon A" uniqKey="Bentwitch S" first="Sharon A" last="Bentwitch">Sharon A. Bentwitch</name><affiliation wicri:level="1"><nlm:affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751</wicri:regionArea><wicri:noRegion>2751</wicri:noRegion></affiliation></author><author><name sortKey="Guillaneuf, Yohann" sort="Guillaneuf, Yohann" uniqKey="Guillaneuf Y" first="Yohann" last="Guillaneuf">Yohann Guillaneuf</name><affiliation wicri:level="4"><nlm:affiliation>CNRS, Institut de Chimie Radicalaire, Aix-Marseille Université, UMR 7273, 13397, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, Institut de Chimie Radicalaire, Aix-Marseille Université, UMR 7273, 13397, Marseille</wicri:regionArea><placeName><settlement type="city">Marseille</settlement><region type="région" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Jones, Mark R" sort="Jones, Mark R" uniqKey="Jones M" first="Mark R" last="Jones">Mark R. Jones</name><affiliation wicri:level="1"><nlm:affiliation>University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>University of Western Sydney, School of Science and Health, Locked Bag 1797, Penrith, New South Wales, 2751</wicri:regionArea><wicri:noRegion>2751</wicri:noRegion></affiliation></author><author><name sortKey="Gaborieau, Marianne" sort="Gaborieau, Marianne" uniqKey="Gaborieau M" first="Marianne" last="Gaborieau">Marianne Gaborieau</name><affiliation wicri:level="1"><nlm:affiliation>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751, Australia. m.gaborieau@uws.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>University of Western Sydney, Molecular Medicine Research Group, Locked Bag 1797, Penrith, New South Wales, 2751</wicri:regionArea><wicri:noRegion>2751</wicri:noRegion></affiliation></author><author><name sortKey="Castignolles, Patrice" sort="Castignolles, Patrice" uniqKey="Castignolles P" first="Patrice" last="Castignolles">Patrice Castignolles</name><affiliation wicri:level="1"><nlm:affiliation>University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>University of Western Sydney, Australian Centre for Research on Separation Science (ACROSS), Locked Bag 1797, Penrith, New South Wales, 2751</wicri:regionArea><wicri:noRegion>2751</wicri:noRegion></affiliation></author></analytic><series><title level="j">Macromolecular bioscience</title><idno type="eISSN">1616-5195</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acrylic Resins (chemistry)</term><term>Animals</term><term>Blood Proteins (chemistry)</term><term>Cell Line, Tumor</term><term>Cell Survival</term><term>Mice</term><term>Myoblasts, Skeletal (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Lignée cellulaire tumorale</term><term>Myoblastes squelettiques (métabolisme)</term><term>Protéines du sang ()</term><term>Résines acryliques ()</term><term>Souris</term><term>Survie cellulaire</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Acrylic Resins</term><term>Blood Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Myoblasts, Skeletal</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Myoblastes squelettiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line, Tumor</term><term>Cell Survival</term><term>Mice</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Lignée cellulaire tumorale</term><term>Protéines du sang</term><term>Résines acryliques</term><term>Souris</term><term>Survie cellulaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Poly(acrylic acid-co-sodium acrylate) (PNaA) is a pH-responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3-arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72 h MTT assay most PNaAs exhibited a IC50 between 7 and 14 mmol L(-1), showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3-arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement><orgName><li>Université d'Aix-Marseille</li></orgName></list><tree><country name="Australie"><noRegion><name sortKey="Whitty, Elizabeth G" sort="Whitty, Elizabeth G" uniqKey="Whitty E" first="Elizabeth G" last="Whitty">Elizabeth G. Whitty</name></noRegion><name sortKey="Bentwitch, Sharon A" sort="Bentwitch, Sharon A" uniqKey="Bentwitch S" first="Sharon A" last="Bentwitch">Sharon A. Bentwitch</name><name sortKey="Castignolles, Patrice" sort="Castignolles, Patrice" uniqKey="Castignolles P" first="Patrice" last="Castignolles">Patrice Castignolles</name><name sortKey="Gaborieau, Marianne" sort="Gaborieau, Marianne" uniqKey="Gaborieau M" first="Marianne" last="Gaborieau">Marianne Gaborieau</name><name sortKey="Jones, Mark R" sort="Jones, Mark R" uniqKey="Jones M" first="Mark R" last="Jones">Mark R. Jones</name><name sortKey="Maniego, Alison R" sort="Maniego, Alison R" uniqKey="Maniego A" first="Alison R" last="Maniego">Alison R. Maniego</name></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Guillaneuf, Yohann" sort="Guillaneuf, Yohann" uniqKey="Guillaneuf Y" first="Yohann" last="Guillaneuf">Yohann Guillaneuf</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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