Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.
Identifieur interne : 001F80 ( Ncbi/Checkpoint ); précédent : 001F79; suivant : 001F81Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.
Auteurs : Asami Makino [Slovénie] ; Mitsuhiro Abe [Slovénie] ; Motohide Murate [Slovénie] ; Takehiko Inaba [Slovénie] ; Neval Yilmaz [Slovénie] ; Françoise Hullin-Matsuda [Slovénie] ; Takuma Kishimoto [Slovénie] ; Nicole L. Schieber [Slovénie] ; Tomohiko Taguchi [Slovénie] ; Hiroyuki Arai [Slovénie] ; Gregor Anderluh [Slovénie] ; Robert G. Parton [Slovénie] ; Toshihide Kobayashi [Japon]Source :
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology [ 1530-6860 ] ; 2015.
Descripteurs français
- KwdFr :
- ADN complémentaire (métabolisme), Animaux, Cellules COS, Cellules HeLa, Cellules épithéliales (cytologie), Cercopithecus aethiops, Cytocinèse (physiologie), Fibroblastes (métabolisme), Humains, Liposomes (métabolisme), Maladie de Niemann-Pick de type A (génétique), Membrane cellulaire (métabolisme), Microscopie confocale, Microscopie immunoélectronique, Microscopie à force atomique, Mâle, Nourrisson, Polarité de la cellule, Protéines recombinantes (métabolisme), Sphingomyéline (métabolisme), Survie cellulaire, Test ELISA.
- MESH :
- cytologie : Cellules épithéliales.
- génétique : Maladie de Niemann-Pick de type A.
- métabolisme : ADN complémentaire, Fibroblastes, Liposomes, Membrane cellulaire, Protéines recombinantes, Sphingomyéline.
- physiologie : Cytocinèse.
- Animaux, Cellules COS, Cellules HeLa, Cercopithecus aethiops, Humains, Microscopie confocale, Microscopie immunoélectronique, Microscopie à force atomique, Mâle, Nourrisson, Polarité de la cellule, Survie cellulaire, Test ELISA.
English descriptors
- KwdEn :
- Animals, COS Cells, Cell Membrane (metabolism), Cell Polarity, Cell Survival, Cercopithecus aethiops, Cytokinesis (physiology), DNA, Complementary (metabolism), Enzyme-Linked Immunosorbent Assay, Epithelial Cells (cytology), Fibroblasts (metabolism), HeLa Cells, Humans, Infant, Liposomes (metabolism), Male, Microscopy, Atomic Force, Microscopy, Confocal, Microscopy, Immunoelectron, Niemann-Pick Disease, Type A (genetics), Recombinant Proteins (metabolism), Sphingomyelins (metabolism).
- MESH :
- chemical , metabolism : DNA, Complementary, Liposomes, Recombinant Proteins, Sphingomyelins.
- cytology : Epithelial Cells.
- genetics : Niemann-Pick Disease, Type A.
- metabolism : Cell Membrane, Fibroblasts.
- physiology : Cytokinesis.
- Animals, COS Cells, Cell Polarity, Cell Survival, Cercopithecus aethiops, Enzyme-Linked Immunosorbent Assay, HeLa Cells, Humans, Infant, Male, Microscopy, Atomic Force, Microscopy, Confocal, Microscopy, Immunoelectron.
Abstract
Sphingomyelin (SM) is a major sphingolipid in mammalian cells and is reported to form specific lipid domains together with cholesterol. However, methods to examine the membrane distribution of SM are limited. We demonstrated in model membranes that fluorescent protein conjugates of 2 specific SM-binding toxins, lysenin (Lys) and equinatoxin II (EqtII), recognize different membrane distributions of SM; Lys exclusively binds clustered SM, whereas EqtII preferentially binds dispersed SM. Freeze-fracture immunoelectron microscopy showed that clustered but not dispersed SM formed lipid domains on the cell surface. Glycolipids and the membrane concentration of SM affect the SM distribution pattern on the plasma membrane. Using derivatives of Lys and EqtII as SM distribution-sensitive probes, we revealed the exclusive accumulation of SM clusters in the midbody at the time of cytokinesis. Interestingly, apical membranes of differentiated epithelial cells exhibited dispersed SM distribution, whereas SM was clustered in basolateral membranes. Clustered but not dispersed SM was absent from the cell surface of acid sphingomyelinase-deficient Niemann-Pick type A cells. These data suggest that both the SM content and membrane distribution are crucial for pathophysiological events bringing therapeutic perspective in the role of SM membrane distribution.
DOI: 10.1096/fj.13-247585
PubMed: 25389132
Affiliations:
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pubmed:25389132Le document en format XML
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<author><name sortKey="Arai, Hiroyuki" sort="Arai, Hiroyuki" uniqKey="Arai H" first="Hiroyuki" last="Arai">Hiroyuki Arai</name>
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<author><name sortKey="Anderluh, Gregor" sort="Anderluh, Gregor" uniqKey="Anderluh G" first="Gregor" last="Anderluh">Gregor Anderluh</name>
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<author><name sortKey="Kobayashi, Toshihide" sort="Kobayashi, Toshihide" uniqKey="Kobayashi T" first="Toshihide" last="Kobayashi">Toshihide Kobayashi</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.</title>
<author><name sortKey="Makino, Asami" sort="Makino, Asami" uniqKey="Makino A" first="Asami" last="Makino">Asami Makino</name>
<affiliation wicri:level="1"><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation>
<country xml:lang="fr">Slovénie</country>
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<author><name sortKey="Abe, Mitsuhiro" sort="Abe, Mitsuhiro" uniqKey="Abe M" first="Mitsuhiro" last="Abe">Mitsuhiro Abe</name>
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<country xml:lang="fr">Slovénie</country>
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<country xml:lang="fr">Slovénie</country>
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<author><name sortKey="Inaba, Takehiko" sort="Inaba, Takehiko" uniqKey="Inaba T" first="Takehiko" last="Inaba">Takehiko Inaba</name>
<affiliation wicri:level="1"><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation>
<country xml:lang="fr">Slovénie</country>
<wicri:regionArea>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana</wicri:regionArea>
<wicri:noRegion>Ljubljana</wicri:noRegion>
</affiliation>
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<author><name sortKey="Yilmaz, Neval" sort="Yilmaz, Neval" uniqKey="Yilmaz N" first="Neval" last="Yilmaz">Neval Yilmaz</name>
<affiliation wicri:level="1"><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation>
<country xml:lang="fr">Slovénie</country>
<wicri:regionArea>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana</wicri:regionArea>
<wicri:noRegion>Ljubljana</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hullin Matsuda, Francoise" sort="Hullin Matsuda, Francoise" uniqKey="Hullin Matsuda F" first="Françoise" last="Hullin-Matsuda">Françoise Hullin-Matsuda</name>
<affiliation wicri:level="1"><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation>
<country xml:lang="fr">Slovénie</country>
<wicri:regionArea>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana</wicri:regionArea>
<wicri:noRegion>Ljubljana</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kishimoto, Takuma" sort="Kishimoto, Takuma" uniqKey="Kishimoto T" first="Takuma" last="Kishimoto">Takuma Kishimoto</name>
<affiliation wicri:level="1"><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation>
<country xml:lang="fr">Slovénie</country>
<wicri:regionArea>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana</wicri:regionArea>
<wicri:noRegion>Ljubljana</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Schieber, Nicole L" sort="Schieber, Nicole L" uniqKey="Schieber N" first="Nicole L" last="Schieber">Nicole L. Schieber</name>
<affiliation wicri:level="1"><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation>
<country xml:lang="fr">Slovénie</country>
<wicri:regionArea>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana</wicri:regionArea>
<wicri:noRegion>Ljubljana</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Taguchi, Tomohiko" sort="Taguchi, Tomohiko" uniqKey="Taguchi T" first="Tomohiko" last="Taguchi">Tomohiko Taguchi</name>
<affiliation wicri:level="1"><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation>
<country xml:lang="fr">Slovénie</country>
<wicri:regionArea>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana</wicri:regionArea>
<wicri:noRegion>Ljubljana</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Arai, Hiroyuki" sort="Arai, Hiroyuki" uniqKey="Arai H" first="Hiroyuki" last="Arai">Hiroyuki Arai</name>
<affiliation wicri:level="1"><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation>
<country xml:lang="fr">Slovénie</country>
<wicri:regionArea>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana</wicri:regionArea>
<wicri:noRegion>Ljubljana</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Anderluh, Gregor" sort="Anderluh, Gregor" uniqKey="Anderluh G" first="Gregor" last="Anderluh">Gregor Anderluh</name>
<affiliation wicri:level="1"><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation>
<country xml:lang="fr">Slovénie</country>
<wicri:regionArea>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana</wicri:regionArea>
<wicri:noRegion>Ljubljana</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Parton, Robert G" sort="Parton, Robert G" uniqKey="Parton R" first="Robert G" last="Parton">Robert G. Parton</name>
<affiliation wicri:level="1"><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation>
<country xml:lang="fr">Slovénie</country>
<wicri:regionArea>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana</wicri:regionArea>
<wicri:noRegion>Ljubljana</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kobayashi, Toshihide" sort="Kobayashi, Toshihide" uniqKey="Kobayashi T" first="Toshihide" last="Kobayashi">Toshihide Kobayashi</name>
<affiliation wicri:level="1"><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia kobayasi@riken.jp.</nlm:affiliation>
<country wicri:rule="url">Japon</country>
<wicri:regionArea>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana</wicri:regionArea>
<wicri:noRegion>Ljubljana</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">FASEB journal : official publication of the Federation of American Societies for Experimental Biology</title>
<idno type="eISSN">1530-6860</idno>
<imprint><date when="2015" type="published">2015</date>
</imprint>
</series>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>COS Cells</term>
<term>Cell Membrane (metabolism)</term>
<term>Cell Polarity</term>
<term>Cell Survival</term>
<term>Cercopithecus aethiops</term>
<term>Cytokinesis (physiology)</term>
<term>DNA, Complementary (metabolism)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Epithelial Cells (cytology)</term>
<term>Fibroblasts (metabolism)</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Infant</term>
<term>Liposomes (metabolism)</term>
<term>Male</term>
<term>Microscopy, Atomic Force</term>
<term>Microscopy, Confocal</term>
<term>Microscopy, Immunoelectron</term>
<term>Niemann-Pick Disease, Type A (genetics)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>Sphingomyelins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (métabolisme)</term>
<term>Animaux</term>
<term>Cellules COS</term>
<term>Cellules HeLa</term>
<term>Cellules épithéliales (cytologie)</term>
<term>Cercopithecus aethiops</term>
<term>Cytocinèse (physiologie)</term>
<term>Fibroblastes (métabolisme)</term>
<term>Humains</term>
<term>Liposomes (métabolisme)</term>
<term>Maladie de Niemann-Pick de type A (génétique)</term>
<term>Membrane cellulaire (métabolisme)</term>
<term>Microscopie confocale</term>
<term>Microscopie immunoélectronique</term>
<term>Microscopie à force atomique</term>
<term>Mâle</term>
<term>Nourrisson</term>
<term>Polarité de la cellule</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>Sphingomyéline (métabolisme)</term>
<term>Survie cellulaire</term>
<term>Test ELISA</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Complementary</term>
<term>Liposomes</term>
<term>Recombinant Proteins</term>
<term>Sphingomyelins</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules épithéliales</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Epithelial Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Niemann-Pick Disease, Type A</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Maladie de Niemann-Pick de type A</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term>
<term>Fibroblasts</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN complémentaire</term>
<term>Fibroblastes</term>
<term>Liposomes</term>
<term>Membrane cellulaire</term>
<term>Protéines recombinantes</term>
<term>Sphingomyéline</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Cytocinèse</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cytokinesis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>COS Cells</term>
<term>Cell Polarity</term>
<term>Cell Survival</term>
<term>Cercopithecus aethiops</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Infant</term>
<term>Male</term>
<term>Microscopy, Atomic Force</term>
<term>Microscopy, Confocal</term>
<term>Microscopy, Immunoelectron</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules COS</term>
<term>Cellules HeLa</term>
<term>Cercopithecus aethiops</term>
<term>Humains</term>
<term>Microscopie confocale</term>
<term>Microscopie immunoélectronique</term>
<term>Microscopie à force atomique</term>
<term>Mâle</term>
<term>Nourrisson</term>
<term>Polarité de la cellule</term>
<term>Survie cellulaire</term>
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<front><div type="abstract" xml:lang="en">Sphingomyelin (SM) is a major sphingolipid in mammalian cells and is reported to form specific lipid domains together with cholesterol. However, methods to examine the membrane distribution of SM are limited. We demonstrated in model membranes that fluorescent protein conjugates of 2 specific SM-binding toxins, lysenin (Lys) and equinatoxin II (EqtII), recognize different membrane distributions of SM; Lys exclusively binds clustered SM, whereas EqtII preferentially binds dispersed SM. Freeze-fracture immunoelectron microscopy showed that clustered but not dispersed SM formed lipid domains on the cell surface. Glycolipids and the membrane concentration of SM affect the SM distribution pattern on the plasma membrane. Using derivatives of Lys and EqtII as SM distribution-sensitive probes, we revealed the exclusive accumulation of SM clusters in the midbody at the time of cytokinesis. Interestingly, apical membranes of differentiated epithelial cells exhibited dispersed SM distribution, whereas SM was clustered in basolateral membranes. Clustered but not dispersed SM was absent from the cell surface of acid sphingomyelinase-deficient Niemann-Pick type A cells. These data suggest that both the SM content and membrane distribution are crucial for pathophysiological events bringing therapeutic perspective in the role of SM membrane distribution.</div>
</front>
</TEI>
<affiliations><list><country><li>Japon</li>
<li>Slovénie</li>
</country>
</list>
<tree><country name="Slovénie"><noRegion><name sortKey="Makino, Asami" sort="Makino, Asami" uniqKey="Makino A" first="Asami" last="Makino">Asami Makino</name>
</noRegion>
<name sortKey="Abe, Mitsuhiro" sort="Abe, Mitsuhiro" uniqKey="Abe M" first="Mitsuhiro" last="Abe">Mitsuhiro Abe</name>
<name sortKey="Anderluh, Gregor" sort="Anderluh, Gregor" uniqKey="Anderluh G" first="Gregor" last="Anderluh">Gregor Anderluh</name>
<name sortKey="Arai, Hiroyuki" sort="Arai, Hiroyuki" uniqKey="Arai H" first="Hiroyuki" last="Arai">Hiroyuki Arai</name>
<name sortKey="Hullin Matsuda, Francoise" sort="Hullin Matsuda, Francoise" uniqKey="Hullin Matsuda F" first="Françoise" last="Hullin-Matsuda">Françoise Hullin-Matsuda</name>
<name sortKey="Inaba, Takehiko" sort="Inaba, Takehiko" uniqKey="Inaba T" first="Takehiko" last="Inaba">Takehiko Inaba</name>
<name sortKey="Kishimoto, Takuma" sort="Kishimoto, Takuma" uniqKey="Kishimoto T" first="Takuma" last="Kishimoto">Takuma Kishimoto</name>
<name sortKey="Murate, Motohide" sort="Murate, Motohide" uniqKey="Murate M" first="Motohide" last="Murate">Motohide Murate</name>
<name sortKey="Parton, Robert G" sort="Parton, Robert G" uniqKey="Parton R" first="Robert G" last="Parton">Robert G. Parton</name>
<name sortKey="Schieber, Nicole L" sort="Schieber, Nicole L" uniqKey="Schieber N" first="Nicole L" last="Schieber">Nicole L. Schieber</name>
<name sortKey="Taguchi, Tomohiko" sort="Taguchi, Tomohiko" uniqKey="Taguchi T" first="Tomohiko" last="Taguchi">Tomohiko Taguchi</name>
<name sortKey="Yilmaz, Neval" sort="Yilmaz, Neval" uniqKey="Yilmaz N" first="Neval" last="Yilmaz">Neval Yilmaz</name>
</country>
<country name="Japon"><noRegion><name sortKey="Kobayashi, Toshihide" sort="Kobayashi, Toshihide" uniqKey="Kobayashi T" first="Toshihide" last="Kobayashi">Toshihide Kobayashi</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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