Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.
Identifieur interne : 003029 ( PubMed/Corpus ); précédent : 003028; suivant : 003030Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.
Auteurs : Asami Makino ; Mitsuhiro Abe ; Motohide Murate ; Takehiko Inaba ; Neval Yilmaz ; Françoise Hullin-Matsuda ; Takuma Kishimoto ; Nicole L. Schieber ; Tomohiko Taguchi ; Hiroyuki Arai ; Gregor Anderluh ; Robert G. Parton ; Toshihide KobayashiSource :
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology [ 1530-6860 ] ; 2015.
English descriptors
- KwdEn :
- Animals, COS Cells, Cell Membrane (metabolism), Cell Polarity, Cell Survival, Cercopithecus aethiops, Cytokinesis (physiology), DNA, Complementary (metabolism), Enzyme-Linked Immunosorbent Assay, Epithelial Cells (cytology), Fibroblasts (metabolism), HeLa Cells, Humans, Infant, Liposomes (metabolism), Male, Microscopy, Atomic Force, Microscopy, Confocal, Microscopy, Immunoelectron, Niemann-Pick Disease, Type A (genetics), Recombinant Proteins (metabolism), Sphingomyelins (metabolism).
- MESH :
- chemical , metabolism : DNA, Complementary, Liposomes, Recombinant Proteins, Sphingomyelins.
- cytology : Epithelial Cells.
- genetics : Niemann-Pick Disease, Type A.
- metabolism : Cell Membrane, Fibroblasts.
- physiology : Cytokinesis.
- Animals, COS Cells, Cell Polarity, Cell Survival, Cercopithecus aethiops, Enzyme-Linked Immunosorbent Assay, HeLa Cells, Humans, Infant, Male, Microscopy, Atomic Force, Microscopy, Confocal, Microscopy, Immunoelectron.
Abstract
Sphingomyelin (SM) is a major sphingolipid in mammalian cells and is reported to form specific lipid domains together with cholesterol. However, methods to examine the membrane distribution of SM are limited. We demonstrated in model membranes that fluorescent protein conjugates of 2 specific SM-binding toxins, lysenin (Lys) and equinatoxin II (EqtII), recognize different membrane distributions of SM; Lys exclusively binds clustered SM, whereas EqtII preferentially binds dispersed SM. Freeze-fracture immunoelectron microscopy showed that clustered but not dispersed SM formed lipid domains on the cell surface. Glycolipids and the membrane concentration of SM affect the SM distribution pattern on the plasma membrane. Using derivatives of Lys and EqtII as SM distribution-sensitive probes, we revealed the exclusive accumulation of SM clusters in the midbody at the time of cytokinesis. Interestingly, apical membranes of differentiated epithelial cells exhibited dispersed SM distribution, whereas SM was clustered in basolateral membranes. Clustered but not dispersed SM was absent from the cell surface of acid sphingomyelinase-deficient Niemann-Pick type A cells. These data suggest that both the SM content and membrane distribution are crucial for pathophysiological events bringing therapeutic perspective in the role of SM membrane distribution.
DOI: 10.1096/fj.13-247585
PubMed: 25389132
Links to Exploration step
pubmed:25389132Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.</title><author><name sortKey="Makino, Asami" sort="Makino, Asami" uniqKey="Makino A" first="Asami" last="Makino">Asami Makino</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Abe, Mitsuhiro" sort="Abe, Mitsuhiro" uniqKey="Abe M" first="Mitsuhiro" last="Abe">Mitsuhiro Abe</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Murate, Motohide" sort="Murate, Motohide" uniqKey="Murate M" first="Motohide" last="Murate">Motohide Murate</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Inaba, Takehiko" sort="Inaba, Takehiko" uniqKey="Inaba T" first="Takehiko" last="Inaba">Takehiko Inaba</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Yilmaz, Neval" sort="Yilmaz, Neval" uniqKey="Yilmaz N" first="Neval" last="Yilmaz">Neval Yilmaz</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Hullin Matsuda, Francoise" sort="Hullin Matsuda, Francoise" uniqKey="Hullin Matsuda F" first="Françoise" last="Hullin-Matsuda">Françoise Hullin-Matsuda</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Kishimoto, Takuma" sort="Kishimoto, Takuma" uniqKey="Kishimoto T" first="Takuma" last="Kishimoto">Takuma Kishimoto</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Schieber, Nicole L" sort="Schieber, Nicole L" uniqKey="Schieber N" first="Nicole L" last="Schieber">Nicole L. Schieber</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Taguchi, Tomohiko" sort="Taguchi, Tomohiko" uniqKey="Taguchi T" first="Tomohiko" last="Taguchi">Tomohiko Taguchi</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Arai, Hiroyuki" sort="Arai, Hiroyuki" uniqKey="Arai H" first="Hiroyuki" last="Arai">Hiroyuki Arai</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Anderluh, Gregor" sort="Anderluh, Gregor" uniqKey="Anderluh G" first="Gregor" last="Anderluh">Gregor Anderluh</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Parton, Robert G" sort="Parton, Robert G" uniqKey="Parton R" first="Robert G" last="Parton">Robert G. Parton</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Kobayashi, Toshihide" sort="Kobayashi, Toshihide" uniqKey="Kobayashi T" first="Toshihide" last="Kobayashi">Toshihide Kobayashi</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia kobayasi@riken.jp.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25389132</idno><idno type="pmid">25389132</idno><idno type="doi">10.1096/fj.13-247585</idno><idno type="wicri:Area/PubMed/Corpus">003029</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003029</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.</title><author><name sortKey="Makino, Asami" sort="Makino, Asami" uniqKey="Makino A" first="Asami" last="Makino">Asami Makino</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Abe, Mitsuhiro" sort="Abe, Mitsuhiro" uniqKey="Abe M" first="Mitsuhiro" last="Abe">Mitsuhiro Abe</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Murate, Motohide" sort="Murate, Motohide" uniqKey="Murate M" first="Motohide" last="Murate">Motohide Murate</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Inaba, Takehiko" sort="Inaba, Takehiko" uniqKey="Inaba T" first="Takehiko" last="Inaba">Takehiko Inaba</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Yilmaz, Neval" sort="Yilmaz, Neval" uniqKey="Yilmaz N" first="Neval" last="Yilmaz">Neval Yilmaz</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Hullin Matsuda, Francoise" sort="Hullin Matsuda, Francoise" uniqKey="Hullin Matsuda F" first="Françoise" last="Hullin-Matsuda">Françoise Hullin-Matsuda</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Kishimoto, Takuma" sort="Kishimoto, Takuma" uniqKey="Kishimoto T" first="Takuma" last="Kishimoto">Takuma Kishimoto</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Schieber, Nicole L" sort="Schieber, Nicole L" uniqKey="Schieber N" first="Nicole L" last="Schieber">Nicole L. Schieber</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Taguchi, Tomohiko" sort="Taguchi, Tomohiko" uniqKey="Taguchi T" first="Tomohiko" last="Taguchi">Tomohiko Taguchi</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Arai, Hiroyuki" sort="Arai, Hiroyuki" uniqKey="Arai H" first="Hiroyuki" last="Arai">Hiroyuki Arai</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Anderluh, Gregor" sort="Anderluh, Gregor" uniqKey="Anderluh G" first="Gregor" last="Anderluh">Gregor Anderluh</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Parton, Robert G" sort="Parton, Robert G" uniqKey="Parton R" first="Robert G" last="Parton">Robert G. Parton</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</nlm:affiliation></affiliation></author><author><name sortKey="Kobayashi, Toshihide" sort="Kobayashi, Toshihide" uniqKey="Kobayashi T" first="Toshihide" last="Kobayashi">Toshihide Kobayashi</name><affiliation><nlm:affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia kobayasi@riken.jp.</nlm:affiliation></affiliation></author></analytic><series><title level="j">FASEB journal : official publication of the Federation of American Societies for Experimental Biology</title><idno type="eISSN">1530-6860</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>COS Cells</term><term>Cell Membrane (metabolism)</term><term>Cell Polarity</term><term>Cell Survival</term><term>Cercopithecus aethiops</term><term>Cytokinesis (physiology)</term><term>DNA, Complementary (metabolism)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epithelial Cells (cytology)</term><term>Fibroblasts (metabolism)</term><term>HeLa Cells</term><term>Humans</term><term>Infant</term><term>Liposomes (metabolism)</term><term>Male</term><term>Microscopy, Atomic Force</term><term>Microscopy, Confocal</term><term>Microscopy, Immunoelectron</term><term>Niemann-Pick Disease, Type A (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>Sphingomyelins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Complementary</term><term>Liposomes</term><term>Recombinant Proteins</term><term>Sphingomyelins</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Epithelial Cells</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Niemann-Pick Disease, Type A</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term><term>Fibroblasts</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cytokinesis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>COS Cells</term><term>Cell Polarity</term><term>Cell Survival</term><term>Cercopithecus aethiops</term><term>Enzyme-Linked Immunosorbent Assay</term><term>HeLa Cells</term><term>Humans</term><term>Infant</term><term>Male</term><term>Microscopy, Atomic Force</term><term>Microscopy, Confocal</term><term>Microscopy, Immunoelectron</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sphingomyelin (SM) is a major sphingolipid in mammalian cells and is reported to form specific lipid domains together with cholesterol. However, methods to examine the membrane distribution of SM are limited. We demonstrated in model membranes that fluorescent protein conjugates of 2 specific SM-binding toxins, lysenin (Lys) and equinatoxin II (EqtII), recognize different membrane distributions of SM; Lys exclusively binds clustered SM, whereas EqtII preferentially binds dispersed SM. Freeze-fracture immunoelectron microscopy showed that clustered but not dispersed SM formed lipid domains on the cell surface. Glycolipids and the membrane concentration of SM affect the SM distribution pattern on the plasma membrane. Using derivatives of Lys and EqtII as SM distribution-sensitive probes, we revealed the exclusive accumulation of SM clusters in the midbody at the time of cytokinesis. Interestingly, apical membranes of differentiated epithelial cells exhibited dispersed SM distribution, whereas SM was clustered in basolateral membranes. Clustered but not dispersed SM was absent from the cell surface of acid sphingomyelinase-deficient Niemann-Pick type A cells. These data suggest that both the SM content and membrane distribution are crucial for pathophysiological events bringing therapeutic perspective in the role of SM membrane distribution.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25389132</PMID><DateCreated><Year>2015</Year><Month>02</Month><Day>04</Day></DateCreated><DateCompleted><Year>2015</Year><Month>04</Month><Day>01</Day></DateCompleted><DateRevised><Year>2015</Year><Month>02</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1530-6860</ISSN><JournalIssue CitedMedium="Internet"><Volume>29</Volume><Issue>2</Issue><PubDate><Year>2015</Year><Month>Feb</Month></PubDate></JournalIssue><Title>FASEB journal : official publication of the Federation of American Societies for Experimental Biology</Title><ISOAbbreviation>FASEB J.</ISOAbbreviation></Journal><ArticleTitle>Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.</ArticleTitle><Pagination><MedlinePgn>477-93</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1096/fj.13-247585</ELocationID><Abstract><AbstractText>Sphingomyelin (SM) is a major sphingolipid in mammalian cells and is reported to form specific lipid domains together with cholesterol. However, methods to examine the membrane distribution of SM are limited. We demonstrated in model membranes that fluorescent protein conjugates of 2 specific SM-binding toxins, lysenin (Lys) and equinatoxin II (EqtII), recognize different membrane distributions of SM; Lys exclusively binds clustered SM, whereas EqtII preferentially binds dispersed SM. Freeze-fracture immunoelectron microscopy showed that clustered but not dispersed SM formed lipid domains on the cell surface. Glycolipids and the membrane concentration of SM affect the SM distribution pattern on the plasma membrane. Using derivatives of Lys and EqtII as SM distribution-sensitive probes, we revealed the exclusive accumulation of SM clusters in the midbody at the time of cytokinesis. Interestingly, apical membranes of differentiated epithelial cells exhibited dispersed SM distribution, whereas SM was clustered in basolateral membranes. Clustered but not dispersed SM was absent from the cell surface of acid sphingomyelinase-deficient Niemann-Pick type A cells. These data suggest that both the SM content and membrane distribution are crucial for pathophysiological events bringing therapeutic perspective in the role of SM membrane distribution.</AbstractText><CopyrightInformation>© FASEB.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Makino</LastName><ForeName>Asami</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Abe</LastName><ForeName>Mitsuhiro</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Murate</LastName><ForeName>Motohide</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Inaba</LastName><ForeName>Takehiko</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yilmaz</LastName><ForeName>Neval</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hullin-Matsuda</LastName><ForeName>Françoise</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kishimoto</LastName><ForeName>Takuma</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schieber</LastName><ForeName>Nicole L</ForeName><Initials>NL</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Taguchi</LastName><ForeName>Tomohiko</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arai</LastName><ForeName>Hiroyuki</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Anderluh</LastName><ForeName>Gregor</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Parton</LastName><ForeName>Robert G</ForeName><Initials>RG</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kobayashi</LastName><ForeName>Toshihide</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>*Lipid Biology Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, Japan; INSERM U1060-Université Lyon, Villeurbanne, France; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, St. Lucia, Queensland, Australia; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan; National Institute of Chemistry, Ljubljana, Slovenia; and Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia kobayasi@riken.jp.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>11</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>FASEB J</MedlineTA><NlmUniqueID>8804484</NlmUniqueID><ISSNLinking>0892-6638</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008081">Liposomes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013109">Sphingomyelins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019556" MajorTopicYN="N">COS Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016764" MajorTopicYN="N">Cell Polarity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Cercopithecus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D048749" MajorTopicYN="N">Cytokinesis</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004847" MajorTopicYN="N">Epithelial Cells</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005347" MajorTopicYN="N">Fibroblasts</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006367" MajorTopicYN="N">HeLa Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007223" MajorTopicYN="N">Infant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008081" MajorTopicYN="N">Liposomes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018625" MajorTopicYN="N">Microscopy, Atomic Force</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016253" MajorTopicYN="N">Microscopy, Immunoelectron</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052536" MajorTopicYN="N">Niemann-Pick Disease, Type A</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013109" MajorTopicYN="N">Sphingomyelins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">lipid binding protein</Keyword><Keyword MajorTopicYN="N">lipid raft</Keyword><Keyword MajorTopicYN="N">membrane lipids</Keyword><Keyword MajorTopicYN="N">sphingolipid</Keyword><Keyword MajorTopicYN="N">toxins</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>11</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>11</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>4</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25389132</ArticleId><ArticleId IdType="pii">fj.13-247585</ArticleId><ArticleId IdType="doi">10.1096/fj.13-247585</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003029 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 003029 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:25389132
|texte= Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:25389132" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |