In situ associations between marine photosynthetic picoeukaryotes and potential parasites - a role for fungi?
Identifieur interne : 001C88 ( Main/Merge ); précédent : 001C87; suivant : 001C89In situ associations between marine photosynthetic picoeukaryotes and potential parasites - a role for fungi?
Auteurs : Cécile Lepère [Royaume-Uni] ; Martin Ostrowski [Australie] ; Manuela Hartmann [Royaume-Uni] ; Mikhail V. Zubkov [Royaume-Uni] ; David J. Scanlan [Royaume-Uni]Source :
- Environmental microbiology reports [ 1758-2229 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Alveolata, Champignons.
- isolement et purification : Alveolata, Champignons.
- microbiologie : Phytoplancton.
- parasitologie : Phytoplancton.
- Biodiversité, Cytométrie en flux, Interactions hôte-parasite, Océan Atlantique, Technique FISH.
English descriptors
- KwdEn :
- MESH :
- genetics : Alveolata, Fungi.
- isolation & purification : Alveolata, Fungi.
- microbiology : Phytoplankton.
- parasitology : Phytoplankton.
- Atlantic Ocean, Biodiversity, Flow Cytometry, Host-Parasite Interactions, In Situ Hybridization, Fluorescence.
Abstract
Photosynthetic picoeukaryotes (PPEs) are important components of the marine picophytoplankton community playing a critical role in CO2 fixation but also as bacterivores, particularly in the oligotrophic gyres. Despite an increased interest in these organisms and an improved understanding of the genetic diversity of this group, we still know little of the environmental factors controlling the abundance of these organisms. Here, we investigated the quantitative importance of eukaryotic parasites in the free-living fraction as well as in associations with PPEs along a transect in the South Atlantic. Using tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH), we provide quantitative evidence of the occurrence of free-living fungi in open ocean marine systems, while the Perkinsozoa and Syndiniales parasites were not abundant in these waters. Using flow cytometric cell sorting of different PPE populations followed by a dual-labelled TSA-FISH approach, we also demonstrate fungal associations, potentially parasitic, occurring with both pico-Prymnesiophyceae and pico-Chrysophyceae. These data highlight the necessity for further work investigating the specific role of marine fungi as parasites of phytoplankton to improve understanding of carbon flow in marine ecosystems.
DOI: 10.1111/1758-2229.12339
PubMed: 26420747
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Scanlan</name><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Life Sciences, University of Warwick, Coventry, CV4 7AL</wicri:regionArea><wicri:noRegion>CV4 7AL</wicri:noRegion></affiliation></author></analytic><series><title level="j">Environmental microbiology reports</title><idno type="eISSN">1758-2229</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alveolata (genetics)</term><term>Alveolata (isolation & purification)</term><term>Atlantic Ocean</term><term>Biodiversity</term><term>Flow Cytometry</term><term>Fungi (genetics)</term><term>Fungi (isolation & purification)</term><term>Host-Parasite Interactions</term><term>In Situ Hybridization, Fluorescence</term><term>Phytoplankton (microbiology)</term><term>Phytoplankton (parasitology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alveolata (génétique)</term><term>Alveolata (isolement et purification)</term><term>Biodiversité</term><term>Champignons (génétique)</term><term>Champignons (isolement et purification)</term><term>Cytométrie en flux</term><term>Interactions hôte-parasite</term><term>Océan Atlantique</term><term>Phytoplancton (microbiologie)</term><term>Phytoplancton (parasitologie)</term><term>Technique FISH</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Alveolata</term><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Alveolata</term><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Alveolata</term><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Alveolata</term><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Phytoplancton</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Phytoplankton</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Phytoplancton</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Phytoplankton</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Atlantic Ocean</term><term>Biodiversity</term><term>Flow Cytometry</term><term>Host-Parasite Interactions</term><term>In Situ Hybridization, Fluorescence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biodiversité</term><term>Cytométrie en flux</term><term>Interactions hôte-parasite</term><term>Océan Atlantique</term><term>Technique FISH</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Photosynthetic picoeukaryotes (PPEs) are important components of the marine picophytoplankton community playing a critical role in CO2 fixation but also as bacterivores, particularly in the oligotrophic gyres. Despite an increased interest in these organisms and an improved understanding of the genetic diversity of this group, we still know little of the environmental factors controlling the abundance of these organisms. Here, we investigated the quantitative importance of eukaryotic parasites in the free-living fraction as well as in associations with PPEs along a transect in the South Atlantic. Using tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH), we provide quantitative evidence of the occurrence of free-living fungi in open ocean marine systems, while the Perkinsozoa and Syndiniales parasites were not abundant in these waters. Using flow cytometric cell sorting of different PPE populations followed by a dual-labelled TSA-FISH approach, we also demonstrate fungal associations, potentially parasitic, occurring with both pico-Prymnesiophyceae and pico-Chrysophyceae. These data highlight the necessity for further work investigating the specific role of marine fungi as parasites of phytoplankton to improve understanding of carbon flow in marine ecosystems.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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