Serveur d'exploration sur les relations entre la France et l'Australie

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Comparative Linkage Mapping of Human Chromosome 13 and Bovine Chromosome 12

Identifieur interne : 00CE71 ( Main/Exploration ); précédent : 00CE70; suivant : 00CE72

Comparative Linkage Mapping of Human Chromosome 13 and Bovine Chromosome 12

Auteurs : H. S. Sun ; L. Cai ; S. K. Davis ; J. F. Taylor ; L. K. Doud ; M. D. Bishop ; H. Hayes [France] ; W. Barendse [Australie] ; D. Vaiman [France] ; R. A. Mcgraw ; T. Hirano [Japon] ; Y. Sugimoto [Japon] ; B. W. Kirkpatrick

Source :

RBID : ISTEX:449D65517AF736073F97D088749C0CDFEC2C6A56

Descripteurs français

English descriptors

Abstract

Abstract: A comparative linkage map of human chromosome 13 and bovine chromosome 12 was constructed using eight polymorphic microsatellite markers associated with six specific genes. Linkage of these was also examined relative to five previously mapped anonymous microsatellite markers. Seven gene-linked markers were developed from bovine large-insert genomic clones containing one of five genes of interest (serotonin receptor subtype 2, fms-related tyrosine kinase, coagulation factor 10, retinoblastoma susceptibility gene, collagen type IV alpha 1), and one additional marker was developed from a microsatellite resident within an intron of the bovine dopachrome tautomerase gene. Four of these loci were previously assigned to bovine chromosome 12 by analysis of a somatic cell hybrid panel. This study provides linkage information for examining gene order in this conserved synteny group. The comparative linkage mapping results indicate that the q arm of human chromosome 13 is almost entirely conserved in bovine chromosome 12. One intrachromosomal rearrangement was detected in this linkage group relative to human, and this rearrangement was confirmed by fluorescencein situhybridization results.

Url:
DOI: 10.1006/geno.1996.4481


Affiliations:


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Le document en format XML

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<term>Recombination frequencies</term>
<term>Recombination frequency</term>
<term>Retinoblastoma susceptibility gene</term>
<term>Sequence information</term>
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<div type="abstract" xml:lang="en">Abstract: A comparative linkage map of human chromosome 13 and bovine chromosome 12 was constructed using eight polymorphic microsatellite markers associated with six specific genes. Linkage of these was also examined relative to five previously mapped anonymous microsatellite markers. Seven gene-linked markers were developed from bovine large-insert genomic clones containing one of five genes of interest (serotonin receptor subtype 2, fms-related tyrosine kinase, coagulation factor 10, retinoblastoma susceptibility gene, collagen type IV alpha 1), and one additional marker was developed from a microsatellite resident within an intron of the bovine dopachrome tautomerase gene. Four of these loci were previously assigned to bovine chromosome 12 by analysis of a somatic cell hybrid panel. This study provides linkage information for examining gene order in this conserved synteny group. The comparative linkage mapping results indicate that the q arm of human chromosome 13 is almost entirely conserved in bovine chromosome 12. One intrachromosomal rearrangement was detected in this linkage group relative to human, and this rearrangement was confirmed by fluorescencein situhybridization results.</div>
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