Biodegradation of free phytol by bacterial communities isolated from marine sediments under aerobic and denitrifying conditions
Identifieur interne : 00C818 ( Main/Exploration ); précédent : 00C817; suivant : 00C819Biodegradation of free phytol by bacterial communities isolated from marine sediments under aerobic and denitrifying conditions
Auteurs : J.-F. Rontani [France] ; P. C. Bonin [France] ; J. K. Volkman [Australie]Source :
- Applied and environmental microbiology : (Print) [ 0099-2240 ] ; 1999.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Milieu marin.
English descriptors
- KwdEn :
Abstract
Biodegradation of (E)-phytol [3,7,11,15-tetramethylhexadec-2(E)-en-1-ol] by two bacterial communities isolated from recent marine sediments under aerobic and denitrifying conditions was studied at 20°C. This isoprenoid alcohol is metabolized efficiently by these two bacterial communities via 6,10,14-trimethylpentadecan-2-one and (E)-phytenic acid. The first step in both aerobic and anaerobic bacterial degradation of (E)-phytol involves the transient production of (E)-phytenal, which in turn can be abiotically converted to 6,10,14-trimethylpentadecan-2-one. Most of the isoprenoid metabolites identified in vitro could be detected in a fresh sediment core collected at the same site as the sediments used for the incubations. Since (E)-phytenal is less sensitive to abiotic degradation at the temperature of the sediments (15°C), the major part of (E)-phytol appeared to be biodegraded in situ via (E)-phytenic acid. (Z)- and (E)-phytenic acids are present in particularly large quantities in the upper section of the core, and their concentrations quickly decrease with depth in the core. This degradation (which takes place without significant production of phytanic acid) is attributed to the involvement of alternating β-decarboxymethylation and β-oxidation reaction sequences induced by denitrifiers. Despite the low nitrate concentration of marine sediments, denitrifying bacteria seem to play a significant role in the mineralization of (E)-phytol.
Affiliations:
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Bonin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratoire d'Océanographie et de Biogéochimie (UMR 6535), Centre d'Océanologie de Marseille (OSU), Campus de Luminy</s1><s2>13288 Marseille</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>13288 Marseille</wicri:noRegion><placeName><settlement type="city">Marseille</settlement><region type="région" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="Volkman, J K" sort="Volkman, J K" uniqKey="Volkman J" first="J. K." last="Volkman">J. K. Volkman</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>CSIRO Marine Research</s1><s2>Hobart, Tasmania 7001</s2><s3>AUS</s3><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>CSIRO Marine Research</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">00-0045924</idno><date when="1999">1999</date><idno type="stanalyst">PASCAL 00-0045924 INIST</idno><idno type="RBID">Pascal:00-0045924</idno><idno type="wicri:Area/PascalFrancis/Corpus">006145</idno><idno type="wicri:Area/PascalFrancis/Curation">000025</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">006130</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">006130</idno><idno type="wicri:doubleKey">0099-2240:1999:Rontani J:biodegradation:of:free</idno><idno type="wicri:Area/Main/Merge">00D843</idno><idno type="wicri:Area/Main/Curation">00C818</idno><idno type="wicri:Area/Main/Exploration">00C818</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Biodegradation of free phytol by bacterial communities isolated from marine sediments under aerobic and denitrifying conditions</title><author><name sortKey="Rontani, J F" sort="Rontani, J F" uniqKey="Rontani J" first="J.-F." last="Rontani">J.-F. Rontani</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Laboratoire d'Océanographie et de Biogéochimie (UMR 6535), Centre d'Océanologie de Marseille (OSU), Campus de Luminy</s1><s2>13288 Marseille</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Bonin, P C" sort="Bonin, P C" uniqKey="Bonin P" first="P. C." last="Bonin">P. C. Bonin</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Laboratoire d'Océanographie et de Biogéochimie (UMR 6535), Centre d'Océanologie de Marseille (OSU), Campus de Luminy</s1><s2>13288 Marseille</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Volkman, J K" sort="Volkman, J K" uniqKey="Volkman J" first="J. K." last="Volkman">J. K. Volkman</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>CSIRO Marine Research</s1><s2>Hobart, Tasmania 7001</s2><s3>AUS</s3><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>CSIRO Marine Research</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Applied and environmental microbiology : (Print)</title><title level="j" type="abbreviated">Appl. environ. microbiol. : (Print)</title><idno type="ISSN">0099-2240</idno><imprint><date when="1999">1999</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Applied and environmental microbiology : (Print)</title><title level="j" type="abbreviated">Appl. environ. microbiol. : (Print)</title><idno type="ISSN">0099-2240</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aerobiosis</term><term>Biodegradation</term><term>Denitrification</term><term>Isoprenoid</term><term>Marine environment</term><term>Metabolic pathway</term><term>Metabolite</term><term>Microbial community</term><term>Sediments</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Isoprénoïde</term><term>Dégradation biologique</term><term>Sédiment</term><term>Aérobiose</term><term>Dénitrification</term><term>Communauté microbienne</term><term>Métabolite</term><term>Voie métabolique</term><term>Milieu marin</term><term>Phytol</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Milieu marin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Biodegradation of (E)-phytol [3,7,11,15-tetramethylhexadec-2(E)-en-1-ol] by two bacterial communities isolated from recent marine sediments under aerobic and denitrifying conditions was studied at 20°C. This isoprenoid alcohol is metabolized efficiently by these two bacterial communities via 6,10,14-trimethylpentadecan-2-one and (E)-phytenic acid. The first step in both aerobic and anaerobic bacterial degradation of (E)-phytol involves the transient production of (E)-phytenal, which in turn can be abiotically converted to 6,10,14-trimethylpentadecan-2-one. Most of the isoprenoid metabolites identified in vitro could be detected in a fresh sediment core collected at the same site as the sediments used for the incubations. Since (E)-phytenal is less sensitive to abiotic degradation at the temperature of the sediments (15°C), the major part of (E)-phytol appeared to be biodegraded in situ via (E)-phytenic acid. (Z)- and (E)-phytenic acids are present in particularly large quantities in the upper section of the core, and their concentrations quickly decrease with depth in the core. This degradation (which takes place without significant production of phytanic acid) is attributed to the involvement of alternating β-decarboxymethylation and β-oxidation reaction sequences induced by denitrifiers. Despite the low nitrate concentration of marine sediments, denitrifying bacteria seem to play a significant role in the mineralization of (E)-phytol.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Rontani, J F" sort="Rontani, J F" uniqKey="Rontani J" first="J.-F." last="Rontani">J.-F. Rontani</name></region><name sortKey="Bonin, P C" sort="Bonin, P C" uniqKey="Bonin P" first="P. C." last="Bonin">P. C. Bonin</name></country><country name="Australie"><noRegion><name sortKey="Volkman, J K" sort="Volkman, J K" uniqKey="Volkman J" first="J. K." last="Volkman">J. K. Volkman</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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