AustralieFrV1, Main, Exploration, bibRecord, 001A68

Nanostructural determination of a lipid bilayer tethered to a gold substrate.

Identifieur interne : 001A68 ( Main/Exploration ); précédent : 001A67; suivant : 001A69

Nanostructural determination of a lipid bilayer tethered to a gold substrate.

Auteurs : Marco Maccarini [France] ; Erik B. Watkins [États-Unis] ; Barry Stidder [France] ; Jean-Pierre Alcaraz [France] ; Bruce A. Cornell [Australie] ; Donald K. Martin [France]

Source :

The European physical journal. E, Soft matter [ 1292-895X ] ; 2016.

RBID : pubmed:27966072

Abstract

Tethered lipid bilayer membranes (tBLM) are planar membranes composed of free lipids and molecules tethered to a solid planar substrate providing a useful model of biological membranes for a wide range of biophysical studies and biotechnological applications. The properties of the tBLM depend on the free lipids and on the chemistry of the tethering molecules. We present a nanoscale characterization of a tBLM composed of deuterated 1,2-dimyristoyl-sn-glycero-3-phosphocholine (d-DMPC) free lipids, benzyl disulfide undecaethylene glycol phytanol (DLP) tethering molecules, and benzyl disulfiide tetraethylene glycol polar spacer molecules (PSM) used to control the areal density of tethering molecules through coadsorption. The use of selected isotopic substitution provides a way to distinguish the conformation and location of the tethered lipids from the free lipids and to elucidate how the two components influence the structure of the tBLM. These findings provide useful information to optimise the insertion of transmembrane proteins into the tethered bilayer system.

DOI: 10.1140/epje/i2016-16123-5
PubMed: 27966072

Affiliations:

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<front><div type="abstract" xml:lang="en">Tethered lipid bilayer membranes (tBLM) are planar membranes composed of free lipids and molecules tethered to a solid planar substrate providing a useful model of biological membranes for a wide range of biophysical studies and biotechnological applications. The properties of the tBLM depend on the free lipids and on the chemistry of the tethering molecules. We present a nanoscale characterization of a tBLM composed of deuterated 1,2-dimyristoyl-sn-glycero-3-phosphocholine (d-DMPC) free lipids, benzyl disulfide undecaethylene glycol phytanol (DLP) tethering molecules, and benzyl disulfiide tetraethylene glycol polar spacer molecules (PSM) used to control the areal density of tethering molecules through coadsorption. The use of selected isotopic substitution provides a way to distinguish the conformation and location of the tethered lipids from the free lipids and to elucidate how the two components influence the structure of the tBLM. These findings provide useful information to optimise the insertion of transmembrane proteins into the tethered bilayer system.</div>
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Serveur d'exploration sur les relations entre la France et l'Australie

Nanostructural determination of a lipid bilayer tethered to a gold substrate.

Nanostructural determination of a lipid bilayer tethered to a gold substrate.

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