Retrospective analyses of fox feces by real-time PCR to identify new endemic areas of Echinococcus multilocularis in France.
Identifieur interne : 001801 ( Main/Exploration ); précédent : 001800; suivant : 001802Retrospective analyses of fox feces by real-time PCR to identify new endemic areas of Echinococcus multilocularis in France.
Auteurs : Gérald Umhang [France] ; Sébastien Comte [France] ; Vanessa Hormaz [France] ; Jean-Marc Boucher [France] ; Vincent Raton [France] ; Stéphanie Favier [France] ; Francis Raoul [France] ; Patrick Giraudoux [France] ; Benoît Combes [France] ; Franck Boué [France]Source :
- Parasitology research [ 1432-1955 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Echinococcus multilocularis (isolement et purification), France (épidémiologie), Fèces (parasitologie), Humains, Maladies endémiques (médecine vétérinaire), Prévalence, Renards (parasitologie), Réaction de polymérisation en chaine en temps réel (médecine vétérinaire), Échinococcose hépatique (médecine vétérinaire), Échinococcose hépatique (parasitologie), Échinococcose hépatique (épidémiologie), Études rétrospectives.
- MESH :
- isolement et purification : Echinococcus multilocularis.
- médecine vétérinaire : Maladies endémiques, Réaction de polymérisation en chaine en temps réel, Échinococcose hépatique.
- parasitologie : Fèces, Renards, Échinococcose hépatique.
- épidémiologie : France, Échinococcose hépatique.
- Animaux, Humains, Prévalence, Études rétrospectives.
English descriptors
- KwdEn :
- Animals, Echinococcosis, Hepatic (epidemiology), Echinococcosis, Hepatic (parasitology), Echinococcosis, Hepatic (veterinary), Echinococcus multilocularis (isolation & purification), Endemic Diseases (veterinary), Feces (parasitology), Foxes (parasitology), France (epidemiology), Humans, Prevalence, Real-Time Polymerase Chain Reaction (veterinary), Retrospective Studies.
- MESH :
- epidemiology : Echinococcosis, Hepatic, France.
- isolation & purification : Echinococcus multilocularis.
- parasitology : Echinococcosis, Hepatic, Feces, Foxes.
- veterinary : Echinococcosis, Hepatic, Endemic Diseases, Real-Time Polymerase Chain Reaction.
- Animals, Humans, Prevalence, Retrospective Studies.
Abstract
Alveolar echinococcosis is considered to be the most serious zoonosis in the Northern Hemisphere's cold or temperate regions. In Europe, the parasite has a sylvatic life cycle based on predator-prey interactions, mainly between red foxes and small rodents. Echinococcus multilocularis has been observed to have spread across Europe over the last three decades. In France, a westward spread of the parasite's known endemic areas has been described. In this study, a retrospective analysis of fox feces by real-time PCR was carried out in four départements not previously investigated and considered free along with two endemic control departments. The fox feces collected from 2000 to 2004 were analyzed by real-time PCR. Fecal prevalence in the two endemic departments of Doubs and Côte d'Or were estimated at 12 % [6.4-20.0 %] and 4.2 % [1.1-10.3 %], respectively. No positive samples were identified among the 72 feces collected in Drôme or the 112 from Allier, which is consistent with the very low expected prevalence should the parasite be present. Three positive samples were recovered in the Seine-Maritime and Hautes-Alpes départements, resulting in a prevalence of 3.5 % [0.7-10.0 %] and 2.5 % [0.5-7.1 %], respectively. From now on, Hautes-Alpes constitutes the new southern border of the endemic areas in France and confirms the southward expansion previously highlighted. Real-time copro-PCR proved useful in identifying new endemic areas even with low prevalence. Due to the spread of E. multilocularis in France and associated zoonotic risk, it is necessary to expand surveillance in order to fully define all the country's endemic areas. On a continental scale, the development and harmonization of surveillance programs are now needed in order to obtain a global overview of the presence of E. multilocularis and to tailor potential countermeasures.
DOI: 10.1007/s00436-016-5220-1
PubMed: 27517858
Affiliations:
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Le document en format XML
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type="wicri:Area/Main/Exploration">001801</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Retrospective analyses of fox feces by real-time PCR to identify new endemic areas of Echinococcus multilocularis in France.</title><author><name sortKey="Umhang, Gerald" sort="Umhang, Gerald" uniqKey="Umhang G" first="Gérald" last="Umhang">Gérald Umhang</name><affiliation wicri:level="3"><nlm:affiliation>ANSES, Nancy Laboratory for Rabies and Wildlife, National Reference Laboratory for Echinococcus spp., Wildlife Surveillance and Eco-epidemiology Unit, Technopôle Agricole et Vétérinaire, B.P. 40009, 54220, Malzéville, France. gerald.umhang@anses.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>ANSES, Nancy Laboratory for Rabies and Wildlife, National Reference Laboratory for Echinococcus spp., Wildlife Surveillance and Eco-epidemiology Unit, Technopôle Agricole et Vétérinaire, B.P. 40009, 54220, Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author><author><name sortKey="Comte, Sebastien" sort="Comte, Sebastien" uniqKey="Comte S" first="Sébastien" last="Comte">Sébastien Comte</name><affiliation wicri:level="3"><nlm:affiliation>Entente de Lutte Interdépartementale contre les Zoonoses (ELIZ), 54220, Malzéville, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Entente de Lutte Interdépartementale contre les Zoonoses (ELIZ), 54220, Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author><author><name sortKey="Hormaz, Vanessa" sort="Hormaz, Vanessa" uniqKey="Hormaz V" first="Vanessa" last="Hormaz">Vanessa Hormaz</name><affiliation wicri:level="3"><nlm:affiliation>ANSES, Nancy Laboratory for Rabies and Wildlife, National Reference Laboratory for Echinococcus spp., Wildlife Surveillance and Eco-epidemiology Unit, Technopôle Agricole et Vétérinaire, B.P. 40009, 54220, Malzéville, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>ANSES, Nancy Laboratory for Rabies and Wildlife, National Reference Laboratory for Echinococcus spp., Wildlife Surveillance and Eco-epidemiology Unit, Technopôle Agricole et Vétérinaire, B.P. 40009, 54220, Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author><author><name sortKey="Boucher, Jean Marc" sort="Boucher, Jean Marc" uniqKey="Boucher J" first="Jean-Marc" last="Boucher">Jean-Marc Boucher</name><affiliation wicri:level="3"><nlm:affiliation>ANSES, Nancy Laboratory for Rabies and Wildlife, National Reference Laboratory for Echinococcus spp., Wildlife Surveillance and Eco-epidemiology Unit, Technopôle Agricole et Vétérinaire, B.P. 40009, 54220, Malzéville, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>ANSES, Nancy Laboratory for Rabies and Wildlife, National Reference Laboratory for Echinococcus spp., Wildlife Surveillance and Eco-epidemiology Unit, Technopôle Agricole et Vétérinaire, B.P. 40009, 54220, Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author><author><name sortKey="Raton, Vincent" sort="Raton, Vincent" uniqKey="Raton V" first="Vincent" last="Raton">Vincent Raton</name><affiliation wicri:level="3"><nlm:affiliation>Entente de Lutte Interdépartementale contre les Zoonoses (ELIZ), 54220, Malzéville, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Entente de Lutte Interdépartementale contre les Zoonoses (ELIZ), 54220, Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author><author><name sortKey="Favier, Stephanie" sort="Favier, Stephanie" uniqKey="Favier S" first="Stéphanie" last="Favier">Stéphanie Favier</name><affiliation wicri:level="3"><nlm:affiliation>Entente de Lutte Interdépartementale contre les Zoonoses (ELIZ), 54220, Malzéville, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Entente de Lutte Interdépartementale contre les Zoonoses (ELIZ), 54220, Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author><author><name sortKey="Raoul, Francis" sort="Raoul, Francis" uniqKey="Raoul F" first="Francis" last="Raoul">Francis Raoul</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Chrono-environnement, UMR 6249 CNRS, Université of Bourgogne Franche-Comté, 16 route de Gray, F-25030, Besançon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Chrono-environnement, UMR 6249 CNRS, Université of Bourgogne Franche-Comté, 16 route de Gray, F-25030, Besançon</wicri:regionArea><placeName><region type="region" nuts="2">Bourgogne-Franche-Comté</region><region type="old region" nuts="2">Franche-Comté</region><settlement type="city">Besançon</settlement></placeName></affiliation></author><author><name sortKey="Giraudoux, Patrick" sort="Giraudoux, Patrick" uniqKey="Giraudoux P" first="Patrick" last="Giraudoux">Patrick Giraudoux</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Chrono-environnement, UMR 6249 CNRS, Université of Bourgogne Franche-Comté, 16 route de Gray, F-25030, Besançon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Chrono-environnement, UMR 6249 CNRS, Université of Bourgogne Franche-Comté, 16 route de Gray, F-25030, Besançon</wicri:regionArea><placeName><region type="region" nuts="2">Bourgogne-Franche-Comté</region><region type="old region" nuts="2">Franche-Comté</region><settlement type="city">Besançon</settlement></placeName></affiliation></author><author><name sortKey="Combes, Benoit" sort="Combes, Benoit" uniqKey="Combes B" first="Benoît" last="Combes">Benoît Combes</name><affiliation wicri:level="3"><nlm:affiliation>Entente de Lutte Interdépartementale contre les Zoonoses (ELIZ), 54220, Malzéville, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Entente de Lutte Interdépartementale contre les Zoonoses (ELIZ), 54220, Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author><author><name sortKey="Boue, Franck" sort="Boue, Franck" uniqKey="Boue F" first="Franck" last="Boué">Franck Boué</name><affiliation wicri:level="3"><nlm:affiliation>ANSES, Nancy Laboratory for Rabies and Wildlife, National Reference Laboratory for Echinococcus spp., Wildlife Surveillance and Eco-epidemiology Unit, Technopôle Agricole et Vétérinaire, B.P. 40009, 54220, Malzéville, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>ANSES, Nancy Laboratory for Rabies and Wildlife, National Reference Laboratory for Echinococcus spp., Wildlife Surveillance and Eco-epidemiology Unit, Technopôle Agricole et Vétérinaire, B.P. 40009, 54220, Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author></analytic><series><title level="j">Parasitology research</title><idno type="eISSN">1432-1955</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Echinococcosis, Hepatic (epidemiology)</term><term>Echinococcosis, Hepatic (parasitology)</term><term>Echinococcosis, Hepatic (veterinary)</term><term>Echinococcus multilocularis (isolation & purification)</term><term>Endemic Diseases (veterinary)</term><term>Feces (parasitology)</term><term>Foxes (parasitology)</term><term>France (epidemiology)</term><term>Humans</term><term>Prevalence</term><term>Real-Time Polymerase Chain Reaction (veterinary)</term><term>Retrospective Studies</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Echinococcus multilocularis (isolement et purification)</term><term>France (épidémiologie)</term><term>Fèces (parasitologie)</term><term>Humains</term><term>Maladies endémiques (médecine vétérinaire)</term><term>Prévalence</term><term>Renards (parasitologie)</term><term>Réaction de polymérisation en chaine en temps réel (médecine vétérinaire)</term><term>Échinococcose hépatique (médecine vétérinaire)</term><term>Échinococcose hépatique (parasitologie)</term><term>Échinococcose hépatique (épidémiologie)</term><term>Études rétrospectives</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Echinococcosis, Hepatic</term><term>France</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Echinococcus multilocularis</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Echinococcus multilocularis</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Maladies endémiques</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Échinococcose hépatique</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Fèces</term><term>Renards</term><term>Échinococcose hépatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Echinococcosis, Hepatic</term><term>Feces</term><term>Foxes</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Echinococcosis, Hepatic</term><term>Endemic Diseases</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>France</term><term>Échinococcose hépatique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Prevalence</term><term>Retrospective Studies</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Prévalence</term><term>Études rétrospectives</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Alveolar echinococcosis is considered to be the most serious zoonosis in the Northern Hemisphere's cold or temperate regions. In Europe, the parasite has a sylvatic life cycle based on predator-prey interactions, mainly between red foxes and small rodents. Echinococcus multilocularis has been observed to have spread across Europe over the last three decades. In France, a westward spread of the parasite's known endemic areas has been described. In this study, a retrospective analysis of fox feces by real-time PCR was carried out in four départements not previously investigated and considered free along with two endemic control departments. The fox feces collected from 2000 to 2004 were analyzed by real-time PCR. Fecal prevalence in the two endemic departments of Doubs and Côte d'Or were estimated at 12 % [6.4-20.0 %] and 4.2 % [1.1-10.3 %], respectively. No positive samples were identified among the 72 feces collected in Drôme or the 112 from Allier, which is consistent with the very low expected prevalence should the parasite be present. Three positive samples were recovered in the Seine-Maritime and Hautes-Alpes départements, resulting in a prevalence of 3.5 % [0.7-10.0 %] and 2.5 % [0.5-7.1 %], respectively. From now on, Hautes-Alpes constitutes the new southern border of the endemic areas in France and confirms the southward expansion previously highlighted. Real-time copro-PCR proved useful in identifying new endemic areas even with low prevalence. Due to the spread of E. multilocularis in France and associated zoonotic risk, it is necessary to expand surveillance in order to fully define all the country's endemic areas. On a continental scale, the development and harmonization of surveillance programs are now needed in order to obtain a global overview of the presence of E. multilocularis and to tailor potential countermeasures.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Bourgogne-Franche-Comté</li><li>Franche-Comté</li><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Besançon</li><li>Malzéville</li></settlement></list><tree><country name="France"><region name="Grand Est"><name sortKey="Umhang, Gerald" sort="Umhang, Gerald" uniqKey="Umhang G" first="Gérald" last="Umhang">Gérald Umhang</name></region><name sortKey="Boucher, Jean Marc" sort="Boucher, Jean Marc" uniqKey="Boucher J" first="Jean-Marc" last="Boucher">Jean-Marc Boucher</name><name sortKey="Boue, Franck" sort="Boue, Franck" uniqKey="Boue F" first="Franck" last="Boué">Franck Boué</name><name sortKey="Combes, Benoit" sort="Combes, Benoit" uniqKey="Combes B" first="Benoît" last="Combes">Benoît Combes</name><name sortKey="Comte, Sebastien" sort="Comte, Sebastien" uniqKey="Comte S" first="Sébastien" last="Comte">Sébastien Comte</name><name sortKey="Favier, Stephanie" sort="Favier, Stephanie" uniqKey="Favier S" first="Stéphanie" last="Favier">Stéphanie Favier</name><name sortKey="Giraudoux, Patrick" sort="Giraudoux, Patrick" uniqKey="Giraudoux P" first="Patrick" last="Giraudoux">Patrick Giraudoux</name><name sortKey="Hormaz, Vanessa" sort="Hormaz, Vanessa" uniqKey="Hormaz V" first="Vanessa" last="Hormaz">Vanessa Hormaz</name><name sortKey="Raoul, Francis" sort="Raoul, Francis" uniqKey="Raoul F" first="Francis" last="Raoul">Francis Raoul</name><name sortKey="Raton, Vincent" sort="Raton, Vincent" uniqKey="Raton V" first="Vincent" last="Raton">Vincent Raton</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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