AustralieFrV1, Main, Exploration, bibRecord, 001277

A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.

Identifieur interne : 001277 ( Main/Exploration ); précédent : 001276; suivant : 001278

A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.

Auteurs : Asami Makino [Japon] ; Mitsuhiro Abe [Japon] ; Reiko Ishitsuka [Japon] ; Motohide Murate [Japon] ; Takuma Kishimoto [Japon] ; Shota Sakai [Japon] ; Françoise Hullin-Matsuda [Japon] ; Yukiko Shimada [Japon] ; Takehiko Inaba [Japon] ; Hideyuki Miyatake [Japon] ; Hideko Tanaka [Japon] ; Atsushi Kurahashi [Japon] ; Chan-Gi Pack [Japon] ; Rinshi S. Kasai [Japon] ; Shuku Kubo [Japon] ; Nicole L. Schieber [Australie] ; Naoshi Dohmae [Japon] ; Naoya Tochio [Japon] ; Kyoji Hagiwara [Japon] ; Yutaka Sasaki [Japon] ; Yoko Aida [Japon] ; Fumihiro Fujimori [Japon] ; Takanori Kigawa [Japon] ; Kozo Nishibori [Japon] ; Robert G. Parton [Australie] ; Akihiro Kusumi [Japon] ; Yasushi Sako [Japon] ; Gregor Anderluh [Slovénie] ; Makoto Yamashita [Japon] ; Tetsuyuki Kobayashi [Japon] ; Peter Greimel [Japon] ; Toshihide Kobayashi [France]

Source :

FASEB journal : official publication of the Federation of American Societies for Experimental Biology [ 1530-6860 ] ; 2017.

RBID : pubmed:27492925

Descripteurs français

KwdFr :
- Cellules HeLa, Cellules cultivées, Cholestérol (métabolisme), Fibroblastes (), Fibroblastes (métabolisme), Grifola (), Humains, Liaison aux protéines, Libération de particules virales, Maladie de Niemann-Pick de type C (métabolisme), Microdomaines membranaires (), Microdomaines membranaires (métabolisme), Microdomaines membranaires (virologie), Protéines fongiques (), Protéines fongiques (métabolisme), Protéines fongiques (pharmacologie), Sites de fixation, Sphingomyéline (métabolisme).
MESH :
- métabolisme : Cholestérol, Fibroblastes, Maladie de Niemann-Pick de type C, Microdomaines membranaires, Protéines fongiques, Sphingomyéline.
- pharmacologie : Protéines fongiques.
- virologie : Microdomaines membranaires.
- Cellules HeLa, Cellules cultivées, Fibroblastes, Grifola, Humains, Liaison aux protéines, Libération de particules virales, Microdomaines membranaires, Protéines fongiques, Sites de fixation.

English descriptors

KwdEn :
- Binding Sites, Cells, Cultured, Cholesterol (metabolism), Fibroblasts (drug effects), Fibroblasts (metabolism), Fungal Proteins (chemistry), Fungal Proteins (metabolism), Fungal Proteins (pharmacology), Grifola (chemistry), HeLa Cells, Humans, Membrane Microdomains (drug effects), Membrane Microdomains (metabolism), Membrane Microdomains (virology), Niemann-Pick Disease, Type C (metabolism), Protein Binding, Sphingomyelins (metabolism), Virus Release.
MESH :
- chemical , chemistry : Fungal Proteins.
- chemical , metabolism : Cholesterol, Fungal Proteins, Sphingomyelins.
- chemistry : Grifola.
- drug effects : Fibroblasts, Membrane Microdomains.
- metabolism : Fibroblasts, Membrane Microdomains, Niemann-Pick Disease, Type C.
- chemical , pharmacology : Fungal Proteins.
- virology : Membrane Microdomains.
- Binding Sites, Cells, Cultured, HeLa Cells, Humans, Protein Binding, Virus Release.

Abstract

We identified a novel, nontoxic mushroom protein that specifically binds to a complex of sphingomyelin (SM), a major sphingolipid in mammalian cells, and cholesterol (Chol). The purified protein, termed nakanori, labeled cell surface domains in an SM- and Chol-dependent manner and decorated specific lipid domains that colocalized with inner leaflet small GTPase H-Ras, but not K-Ras. The use of nakanori as a lipid-domain-specific probe revealed altered distribution and dynamics of SM/Chol on the cell surface of Niemann-Pick type C fibroblasts, possibly explaining some of the disease phenotype. In addition, that nakanori treatment of epithelial cells after influenza virus infection potently inhibited virus release demonstrates the therapeutic value of targeting specific lipid domains for anti-viral treatment.-Makino, A., Abe, M., Ishitsuka, R., Murate, M., Kishimoto, T., Sakai, S., Hullin-Matsuda, F., Shimada, Y., Inaba, T., Miyatake, H., Tanaka, H., Kurahashi, A., Pack, C.-G., Kasai, R. S., Kubo, S., Schieber, N. L., Dohmae, N., Tochio, N., Hagiwara, K., Sasaki, Y., Aida, Y., Fujimori, F., Kigawa, T., Nishibori, K., Parton, R. G., Kusumi, A., Sako, Y., Anderluh, G., Yamashita, M., Kobayashi, T., Greimel, P., Kobayashi, T. A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.

DOI: 10.1096/fj.201500075R
PubMed: 27492925

Affiliations:

Le document en format XML

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<author><name sortKey="Hullin Matsuda, Francoise" sort="Hullin Matsuda, Francoise" uniqKey="Hullin Matsuda F" first="Françoise" last="Hullin-Matsuda">Françoise Hullin-Matsuda</name>
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<author><name sortKey="Shimada, Yukiko" sort="Shimada, Yukiko" uniqKey="Shimada Y" first="Yukiko" last="Shimada">Yukiko Shimada</name>
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<author><name sortKey="Inaba, Takehiko" sort="Inaba, Takehiko" uniqKey="Inaba T" first="Takehiko" last="Inaba">Takehiko Inaba</name>
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<author><name sortKey="Miyatake, Hideyuki" sort="Miyatake, Hideyuki" uniqKey="Miyatake H" first="Hideyuki" last="Miyatake">Hideyuki Miyatake</name>
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<author><name sortKey="Tanaka, Hideko" sort="Tanaka, Hideko" uniqKey="Tanaka H" first="Hideko" last="Tanaka">Hideko Tanaka</name>
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<author><name sortKey="Kurahashi, Atsushi" sort="Kurahashi, Atsushi" uniqKey="Kurahashi A" first="Atsushi" last="Kurahashi">Atsushi Kurahashi</name>
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<author><name sortKey="Pack, Chan Gi" sort="Pack, Chan Gi" uniqKey="Pack C" first="Chan-Gi" last="Pack">Chan-Gi Pack</name>
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<author><name sortKey="Kasai, Rinshi S" sort="Kasai, Rinshi S" uniqKey="Kasai R" first="Rinshi S" last="Kasai">Rinshi S. Kasai</name>
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<author><name sortKey="Kubo, Shuku" sort="Kubo, Shuku" uniqKey="Kubo S" first="Shuku" last="Kubo">Shuku Kubo</name>
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<author><name sortKey="Schieber, Nicole L" sort="Schieber, Nicole L" uniqKey="Schieber N" first="Nicole L" last="Schieber">Nicole L. Schieber</name>
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<author><name sortKey="Dohmae, Naoshi" sort="Dohmae, Naoshi" uniqKey="Dohmae N" first="Naoshi" last="Dohmae">Naoshi Dohmae</name>
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<author><name sortKey="Tochio, Naoya" sort="Tochio, Naoya" uniqKey="Tochio N" first="Naoya" last="Tochio">Naoya Tochio</name>
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</author>
<author><name sortKey="Hagiwara, Kyoji" sort="Hagiwara, Kyoji" uniqKey="Hagiwara K" first="Kyoji" last="Hagiwara">Kyoji Hagiwara</name>
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<author><name sortKey="Sasaki, Yutaka" sort="Sasaki, Yutaka" uniqKey="Sasaki Y" first="Yutaka" last="Sasaki">Yutaka Sasaki</name>
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<author><name sortKey="Aida, Yoko" sort="Aida, Yoko" uniqKey="Aida Y" first="Yoko" last="Aida">Yoko Aida</name>
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</author>
<author><name sortKey="Fujimori, Fumihiro" sort="Fujimori, Fumihiro" uniqKey="Fujimori F" first="Fumihiro" last="Fujimori">Fumihiro Fujimori</name>
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<author><name sortKey="Kigawa, Takanori" sort="Kigawa, Takanori" uniqKey="Kigawa T" first="Takanori" last="Kigawa">Takanori Kigawa</name>
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</affiliation>
</author>
<author><name sortKey="Nishibori, Kozo" sort="Nishibori, Kozo" uniqKey="Nishibori K" first="Kozo" last="Nishibori">Kozo Nishibori</name>
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<wicri:regionArea>Yukiguni Maitake Co., Limited, Niigata</wicri:regionArea>
<wicri:noRegion>Niigata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Parton, Robert G" sort="Parton, Robert G" uniqKey="Parton R" first="Robert G" last="Parton">Robert G. Parton</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland</wicri:regionArea>
<wicri:noRegion>Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kusumi, Akihiro" sort="Kusumi, Akihiro" uniqKey="Kusumi A" first="Akihiro" last="Kusumi">Akihiro Kusumi</name>
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<country xml:lang="fr">Japon</country>
<wicri:regionArea>Institute for Frontier Medical Sciences, Kyoto University, Kyoto</wicri:regionArea>
<orgName type="university">Université de Kyoto</orgName>
<placeName><settlement type="city">Kyoto</settlement>
<region type="prefecture">Région du Kansai</region>
</placeName>
</affiliation>
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<author><name sortKey="Sako, Yasushi" sort="Sako, Yasushi" uniqKey="Sako Y" first="Yasushi" last="Sako">Yasushi Sako</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Anderluh, Gregor" sort="Anderluh, Gregor" uniqKey="Anderluh G" first="Gregor" last="Anderluh">Gregor Anderluh</name>
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<country xml:lang="fr">Slovénie</country>
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</author>
<author><name sortKey="Yamashita, Makoto" sort="Yamashita, Makoto" uniqKey="Yamashita M" first="Makoto" last="Yamashita">Makoto Yamashita</name>
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</placeName>
</affiliation>
</author>
<author><name sortKey="Kobayashi, Tetsuyuki" sort="Kobayashi, Tetsuyuki" uniqKey="Kobayashi T" first="Tetsuyuki" last="Kobayashi">Tetsuyuki Kobayashi</name>
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</placeName>
</affiliation>
</author>
<author><name sortKey="Greimel, Peter" sort="Greimel, Peter" uniqKey="Greimel P" first="Peter" last="Greimel">Peter Greimel</name>
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<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kobayashi, Toshihide" sort="Kobayashi, Toshihide" uniqKey="Kobayashi T" first="Toshihide" last="Kobayashi">Toshihide Kobayashi</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan; toshihide.kobayashi@unistra.fr.</nlm:affiliation>
<country wicri:rule="url">France</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.</title>
<author><name sortKey="Makino, Asami" sort="Makino, Asami" uniqKey="Makino A" first="Asami" last="Makino">Asami Makino</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Abe, Mitsuhiro" sort="Abe, Mitsuhiro" uniqKey="Abe M" first="Mitsuhiro" last="Abe">Mitsuhiro Abe</name>
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<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ishitsuka, Reiko" sort="Ishitsuka, Reiko" uniqKey="Ishitsuka R" first="Reiko" last="Ishitsuka">Reiko Ishitsuka</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Murate, Motohide" sort="Murate, Motohide" uniqKey="Murate M" first="Motohide" last="Murate">Motohide Murate</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kishimoto, Takuma" sort="Kishimoto, Takuma" uniqKey="Kishimoto T" first="Takuma" last="Kishimoto">Takuma Kishimoto</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sakai, Shota" sort="Sakai, Shota" uniqKey="Sakai S" first="Shota" last="Sakai">Shota Sakai</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hullin Matsuda, Francoise" sort="Hullin Matsuda, Francoise" uniqKey="Hullin Matsuda F" first="Françoise" last="Hullin-Matsuda">Françoise Hullin-Matsuda</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Shimada, Yukiko" sort="Shimada, Yukiko" uniqKey="Shimada Y" first="Yukiko" last="Shimada">Yukiko Shimada</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Inaba, Takehiko" sort="Inaba, Takehiko" uniqKey="Inaba T" first="Takehiko" last="Inaba">Takehiko Inaba</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Miyatake, Hideyuki" sort="Miyatake, Hideyuki" uniqKey="Miyatake H" first="Hideyuki" last="Miyatake">Hideyuki Miyatake</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tanaka, Hideko" sort="Tanaka, Hideko" uniqKey="Tanaka H" first="Hideko" last="Tanaka">Hideko Tanaka</name>
<affiliation wicri:level="3"><nlm:affiliation>Faculty of Core Research, Natural Science Division, Ochanomizu University, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Faculty of Core Research, Natural Science Division, Ochanomizu University, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kurahashi, Atsushi" sort="Kurahashi, Atsushi" uniqKey="Kurahashi A" first="Atsushi" last="Kurahashi">Atsushi Kurahashi</name>
<affiliation wicri:level="1"><nlm:affiliation>Yukiguni Maitake Co., Limited, Niigata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Yukiguni Maitake Co., Limited, Niigata</wicri:regionArea>
<wicri:noRegion>Niigata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pack, Chan Gi" sort="Pack, Chan Gi" uniqKey="Pack C" first="Chan-Gi" last="Pack">Chan-Gi Pack</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kasai, Rinshi S" sort="Kasai, Rinshi S" uniqKey="Kasai R" first="Rinshi S" last="Kasai">Rinshi S. Kasai</name>
<affiliation wicri:level="4"><nlm:affiliation>Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Institute for Frontier Medical Sciences, Kyoto University, Kyoto</wicri:regionArea>
<orgName type="university">Université de Kyoto</orgName>
<placeName><settlement type="city">Kyoto</settlement>
<region type="prefecture">Région du Kansai</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kubo, Shuku" sort="Kubo, Shuku" uniqKey="Kubo S" first="Shuku" last="Kubo">Shuku Kubo</name>
<affiliation wicri:level="3"><nlm:affiliation>Daiichi Sankyo Co., Limited, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Daiichi Sankyo Co., Limited, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Schieber, Nicole L" sort="Schieber, Nicole L" uniqKey="Schieber N" first="Nicole L" last="Schieber">Nicole L. Schieber</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland</wicri:regionArea>
<wicri:noRegion>Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dohmae, Naoshi" sort="Dohmae, Naoshi" uniqKey="Dohmae N" first="Naoshi" last="Dohmae">Naoshi Dohmae</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tochio, Naoya" sort="Tochio, Naoya" uniqKey="Tochio N" first="Naoya" last="Tochio">Naoya Tochio</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hagiwara, Kyoji" sort="Hagiwara, Kyoji" uniqKey="Hagiwara K" first="Kyoji" last="Hagiwara">Kyoji Hagiwara</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sasaki, Yutaka" sort="Sasaki, Yutaka" uniqKey="Sasaki Y" first="Yutaka" last="Sasaki">Yutaka Sasaki</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Aida, Yoko" sort="Aida, Yoko" uniqKey="Aida Y" first="Yoko" last="Aida">Yoko Aida</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fujimori, Fumihiro" sort="Fujimori, Fumihiro" uniqKey="Fujimori F" first="Fumihiro" last="Fujimori">Fumihiro Fujimori</name>
<affiliation wicri:level="3"><nlm:affiliation>Graduate School of Humanities and Life Sciences, Tokyo Kasei University, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Graduate School of Humanities and Life Sciences, Tokyo Kasei University, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kigawa, Takanori" sort="Kigawa, Takanori" uniqKey="Kigawa T" first="Takanori" last="Kigawa">Takanori Kigawa</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nishibori, Kozo" sort="Nishibori, Kozo" uniqKey="Nishibori K" first="Kozo" last="Nishibori">Kozo Nishibori</name>
<affiliation wicri:level="1"><nlm:affiliation>Yukiguni Maitake Co., Limited, Niigata, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Yukiguni Maitake Co., Limited, Niigata</wicri:regionArea>
<wicri:noRegion>Niigata</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Parton, Robert G" sort="Parton, Robert G" uniqKey="Parton R" first="Robert G" last="Parton">Robert G. Parton</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland</wicri:regionArea>
<wicri:noRegion>Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kusumi, Akihiro" sort="Kusumi, Akihiro" uniqKey="Kusumi A" first="Akihiro" last="Kusumi">Akihiro Kusumi</name>
<affiliation wicri:level="4"><nlm:affiliation>Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Institute for Frontier Medical Sciences, Kyoto University, Kyoto</wicri:regionArea>
<orgName type="university">Université de Kyoto</orgName>
<placeName><settlement type="city">Kyoto</settlement>
<region type="prefecture">Région du Kansai</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sako, Yasushi" sort="Sako, Yasushi" uniqKey="Sako Y" first="Yasushi" last="Sako">Yasushi Sako</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Anderluh, Gregor" sort="Anderluh, Gregor" uniqKey="Anderluh G" first="Gregor" last="Anderluh">Gregor Anderluh</name>
<affiliation wicri:level="1"><nlm:affiliation>National Institute of Chemistry, Ljubljana, Slovenia.</nlm:affiliation>
<country xml:lang="fr">Slovénie</country>
<wicri:regionArea>National Institute of Chemistry, Ljubljana</wicri:regionArea>
<wicri:noRegion>Ljubljana</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yamashita, Makoto" sort="Yamashita, Makoto" uniqKey="Yamashita M" first="Makoto" last="Yamashita">Makoto Yamashita</name>
<affiliation wicri:level="3"><nlm:affiliation>Daiichi Sankyo Co., Limited, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Daiichi Sankyo Co., Limited, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kobayashi, Tetsuyuki" sort="Kobayashi, Tetsuyuki" uniqKey="Kobayashi T" first="Tetsuyuki" last="Kobayashi">Tetsuyuki Kobayashi</name>
<affiliation wicri:level="3"><nlm:affiliation>Faculty of Core Research, Natural Science Division, Ochanomizu University, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Faculty of Core Research, Natural Science Division, Ochanomizu University, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Greimel, Peter" sort="Greimel, Peter" uniqKey="Greimel P" first="Peter" last="Greimel">Peter Greimel</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kobayashi, Toshihide" sort="Kobayashi, Toshihide" uniqKey="Kobayashi T" first="Toshihide" last="Kobayashi">Toshihide Kobayashi</name>
<affiliation wicri:level="1"><nlm:affiliation>Rikagaku Kenkyūsho (RIKEN), Saitama, Japan; toshihide.kobayashi@unistra.fr.</nlm:affiliation>
<country wicri:rule="url">France</country>
<wicri:regionArea>Rikagaku Kenkyūsho (RIKEN), Saitama</wicri:regionArea>
<wicri:noRegion>Saitama</wicri:noRegion>
<wicri:noRegion>Saitama</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">FASEB journal : official publication of the Federation of American Societies for Experimental Biology</title>
<idno type="eISSN">1530-6860</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Binding Sites</term>
<term>Cells, Cultured</term>
<term>Cholesterol (metabolism)</term>
<term>Fibroblasts (drug effects)</term>
<term>Fibroblasts (metabolism)</term>
<term>Fungal Proteins (chemistry)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Fungal Proteins (pharmacology)</term>
<term>Grifola (chemistry)</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Membrane Microdomains (drug effects)</term>
<term>Membrane Microdomains (metabolism)</term>
<term>Membrane Microdomains (virology)</term>
<term>Niemann-Pick Disease, Type C (metabolism)</term>
<term>Protein Binding</term>
<term>Sphingomyelins (metabolism)</term>
<term>Virus Release</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Cellules HeLa</term>
<term>Cellules cultivées</term>
<term>Cholestérol (métabolisme)</term>
<term>Fibroblastes ()</term>
<term>Fibroblastes (métabolisme)</term>
<term>Grifola ()</term>
<term>Humains</term>
<term>Liaison aux protéines</term>
<term>Libération de particules virales</term>
<term>Maladie de Niemann-Pick  de type C (métabolisme)</term>
<term>Microdomaines membranaires ()</term>
<term>Microdomaines membranaires (métabolisme)</term>
<term>Microdomaines membranaires (virologie)</term>
<term>Protéines fongiques ()</term>
<term>Protéines fongiques (métabolisme)</term>
<term>Protéines fongiques (pharmacologie)</term>
<term>Sites de fixation</term>
<term>Sphingomyéline (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cholesterol</term>
<term>Fungal Proteins</term>
<term>Sphingomyelins</term>
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</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Fibroblasts</term>
<term>Membrane Microdomains</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fibroblasts</term>
<term>Membrane Microdomains</term>
<term>Niemann-Pick Disease, Type C</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cholestérol</term>
<term>Fibroblastes</term>
<term>Maladie de Niemann-Pick  de type C</term>
<term>Microdomaines membranaires</term>
<term>Protéines fongiques</term>
<term>Sphingomyéline</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Protéines fongiques</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Fungal Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Microdomaines membranaires</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Membrane Microdomains</term>
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<term>Cells, Cultured</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Protein Binding</term>
<term>Virus Release</term>
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<term>Cellules cultivées</term>
<term>Fibroblastes</term>
<term>Grifola</term>
<term>Humains</term>
<term>Liaison aux protéines</term>
<term>Libération de particules virales</term>
<term>Microdomaines membranaires</term>
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<front><div type="abstract" xml:lang="en">We identified a novel, nontoxic mushroom protein that specifically binds to a complex of sphingomyelin (SM), a major sphingolipid in mammalian cells, and cholesterol (Chol). The purified protein, termed nakanori, labeled cell surface domains in an SM- and Chol-dependent manner and decorated specific lipid domains that colocalized with inner leaflet small GTPase H-Ras, but not K-Ras. The use of nakanori as a lipid-domain-specific probe revealed altered distribution and dynamics of SM/Chol on the cell surface of Niemann-Pick type C fibroblasts, possibly explaining some of the disease phenotype. In addition, that nakanori treatment of epithelial cells after influenza virus infection potently inhibited virus release demonstrates the therapeutic value of targeting specific lipid domains for anti-viral treatment.-Makino, A., Abe, M., Ishitsuka, R., Murate, M., Kishimoto, T., Sakai, S., Hullin-Matsuda, F., Shimada, Y., Inaba, T., Miyatake, H., Tanaka, H., Kurahashi, A., Pack, C.-G., Kasai, R. S., Kubo, S., Schieber, N. L., Dohmae, N., Tochio, N., Hagiwara, K., Sasaki, Y., Aida, Y., Fujimori, F., Kigawa, T., Nishibori, K., Parton, R. G., Kusumi, A., Sako, Y., Anderluh, G., Yamashita, M., Kobayashi, T., Greimel, P., Kobayashi, T. A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>Japon</li>
<li>Slovénie</li>
</country>
<region><li>Région de Kantō</li>
<li>Région du Kansai</li>
</region>
<settlement><li>Kyoto</li>
<li>Tokyo</li>
</settlement>
<orgName><li>Université de Kyoto</li>
</orgName>
</list>
<tree><country name="Japon"><noRegion><name sortKey="Makino, Asami" sort="Makino, Asami" uniqKey="Makino A" first="Asami" last="Makino">Asami Makino</name>
</noRegion>
<name sortKey="Abe, Mitsuhiro" sort="Abe, Mitsuhiro" uniqKey="Abe M" first="Mitsuhiro" last="Abe">Mitsuhiro Abe</name>
<name sortKey="Aida, Yoko" sort="Aida, Yoko" uniqKey="Aida Y" first="Yoko" last="Aida">Yoko Aida</name>
<name sortKey="Dohmae, Naoshi" sort="Dohmae, Naoshi" uniqKey="Dohmae N" first="Naoshi" last="Dohmae">Naoshi Dohmae</name>
<name sortKey="Fujimori, Fumihiro" sort="Fujimori, Fumihiro" uniqKey="Fujimori F" first="Fumihiro" last="Fujimori">Fumihiro Fujimori</name>
<name sortKey="Greimel, Peter" sort="Greimel, Peter" uniqKey="Greimel P" first="Peter" last="Greimel">Peter Greimel</name>
<name sortKey="Hagiwara, Kyoji" sort="Hagiwara, Kyoji" uniqKey="Hagiwara K" first="Kyoji" last="Hagiwara">Kyoji Hagiwara</name>
<name sortKey="Hullin Matsuda, Francoise" sort="Hullin Matsuda, Francoise" uniqKey="Hullin Matsuda F" first="Françoise" last="Hullin-Matsuda">Françoise Hullin-Matsuda</name>
<name sortKey="Inaba, Takehiko" sort="Inaba, Takehiko" uniqKey="Inaba T" first="Takehiko" last="Inaba">Takehiko Inaba</name>
<name sortKey="Ishitsuka, Reiko" sort="Ishitsuka, Reiko" uniqKey="Ishitsuka R" first="Reiko" last="Ishitsuka">Reiko Ishitsuka</name>
<name sortKey="Kasai, Rinshi S" sort="Kasai, Rinshi S" uniqKey="Kasai R" first="Rinshi S" last="Kasai">Rinshi S. Kasai</name>
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   |texte=   A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.
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Serveur d'exploration sur les relations entre la France et l'Australie

A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.

A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.

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	Serveur d'exploration sur les relations entre la France et l'Australie
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