Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy.
Identifieur interne : 000059 ( Main/Curation ); précédent : 000058; suivant : 000060Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy.
Auteurs : Haolu Wang [Australie] ; Xiaowen Liang [Australie] ; Germain Gravot [France] ; Camilla A. Thorling [Australie] ; Darrell H G. Crawford [Australie] ; Zhi Ping Xu [Australie] ; Xin Liu [Australie] ; Michael S. Roberts [Australie]Source :
- Journal of biophotonics [ 1864-0648 ] ; 2017.
Abstract
Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity, which helps us to better understand the cellular morphology, microenvironment, immune responses and spatiotemporal dynamics of drugs and therapeutic cells in the healthy and diseased liver. This review summarizes the principles, opportunities, applications and limitations of MPM in hepatology. A key emphasis is on the use of fluorescence lifetime imaging (FLIM) to add additional quantification and specificity to the detection of endogenous fluorescent species in the liver as well as exogenous molecules and nanoparticles that are applied to the liver in vivo. We anticipate that in the near future MPM-FLIM will advance our understanding of the cellular and molecular mechanisms of liver diseases, and will be evaluated from bench to bedside, leading to real-time histology of human liver diseases.
DOI: 10.1002/jbio.201600083
PubMed: 27312349
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Thorling</name><affiliation wicri:level="1"><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102</wicri:regionArea><wicri:noRegion>QLD 4102</wicri:noRegion></affiliation></author><author><name sortKey="Crawford, Darrell H G" sort="Crawford, Darrell H G" uniqKey="Crawford D" first="Darrell H G" last="Crawford">Darrell H G. Crawford</name><affiliation wicri:level="1"><nlm:affiliation>School of Medicine, The University of Queensland, Gallipoli Medical Research Foundation, Greenslopes Private Hospital, Greenslopes, QLD 4120, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of Medicine, The University of Queensland, Gallipoli Medical Research Foundation, Greenslopes Private Hospital, Greenslopes, QLD 4120</wicri:regionArea><wicri:noRegion>QLD 4120</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Zhi Ping" sort="Xu, Zhi Ping" uniqKey="Xu Z" first="Zhi Ping" last="Xu">Zhi Ping Xu</name><affiliation wicri:level="1"><nlm:affiliation>Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072</wicri:regionArea><wicri:noRegion>QLD 4072</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Xin" sort="Liu, Xin" uniqKey="Liu X" first="Xin" last="Liu">Xin Liu</name><affiliation wicri:level="1"><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102</wicri:regionArea><wicri:noRegion>QLD 4102</wicri:noRegion></affiliation></author><author><name sortKey="Roberts, Michael S" sort="Roberts, Michael S" uniqKey="Roberts M" first="Michael S" last="Roberts">Michael S. Roberts</name><affiliation wicri:level="1"><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102</wicri:regionArea><wicri:noRegion>QLD 4102</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of biophotonics</title><idno type="eISSN">1864-0648</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity, which helps us to better understand the cellular morphology, microenvironment, immune responses and spatiotemporal dynamics of drugs and therapeutic cells in the healthy and diseased liver. This review summarizes the principles, opportunities, applications and limitations of MPM in hepatology. A key emphasis is on the use of fluorescence lifetime imaging (FLIM) to add additional quantification and specificity to the detection of endogenous fluorescent species in the liver as well as exogenous molecules and nanoparticles that are applied to the liver in vivo. We anticipate that in the near future MPM-FLIM will advance our understanding of the cellular and molecular mechanisms of liver diseases, and will be evaluated from bench to bedside, leading to real-time histology of human liver diseases.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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