Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy.
Identifieur interne : 001385 ( PubMed/Corpus ); précédent : 001384; suivant : 001386Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy.
Auteurs : Haolu Wang ; Xiaowen Liang ; Germain Gravot ; Camilla A. Thorling ; Darrell H G. Crawford ; Zhi Ping Xu ; Xin Liu ; Michael S. RobertsSource :
- Journal of biophotonics [ 1864-0648 ] ; 2017.
Abstract
Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity, which helps us to better understand the cellular morphology, microenvironment, immune responses and spatiotemporal dynamics of drugs and therapeutic cells in the healthy and diseased liver. This review summarizes the principles, opportunities, applications and limitations of MPM in hepatology. A key emphasis is on the use of fluorescence lifetime imaging (FLIM) to add additional quantification and specificity to the detection of endogenous fluorescent species in the liver as well as exogenous molecules and nanoparticles that are applied to the liver in vivo. We anticipate that in the near future MPM-FLIM will advance our understanding of the cellular and molecular mechanisms of liver diseases, and will be evaluated from bench to bedside, leading to real-time histology of human liver diseases.
DOI: 10.1002/jbio.201600083
PubMed: 27312349
Links to Exploration step
pubmed:27312349Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy.</title><author><name sortKey="Wang, Haolu" sort="Wang, Haolu" uniqKey="Wang H" first="Haolu" last="Wang">Haolu Wang</name><affiliation><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Liang, Xiaowen" sort="Liang, Xiaowen" uniqKey="Liang X" first="Xiaowen" last="Liang">Xiaowen Liang</name><affiliation><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Gravot, Germain" sort="Gravot, Germain" uniqKey="Gravot G" first="Germain" last="Gravot">Germain Gravot</name><affiliation><nlm:affiliation>Department of Pharmacy, University of Rennes 1, Ille-et-Vilaine, Rennes, 35043, France.</nlm:affiliation></affiliation></author><author><name sortKey="Thorling, Camilla A" sort="Thorling, Camilla A" uniqKey="Thorling C" first="Camilla A" last="Thorling">Camilla A. Thorling</name><affiliation><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Crawford, Darrell H G" sort="Crawford, Darrell H G" uniqKey="Crawford D" first="Darrell H G" last="Crawford">Darrell H G. Crawford</name><affiliation><nlm:affiliation>School of Medicine, The University of Queensland, Gallipoli Medical Research Foundation, Greenslopes Private Hospital, Greenslopes, QLD 4120, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Zhi Ping" sort="Xu, Zhi Ping" uniqKey="Xu Z" first="Zhi Ping" last="Xu">Zhi Ping Xu</name><affiliation><nlm:affiliation>Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Xin" sort="Liu, Xin" uniqKey="Liu X" first="Xin" last="Liu">Xin Liu</name><affiliation><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Roberts, Michael S" sort="Roberts, Michael S" uniqKey="Roberts M" first="Michael S" last="Roberts">Michael S. Roberts</name><affiliation><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:27312349</idno><idno type="pmid">27312349</idno><idno type="doi">10.1002/jbio.201600083</idno><idno type="wicri:Area/PubMed/Corpus">001385</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001385</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy.</title><author><name sortKey="Wang, Haolu" sort="Wang, Haolu" uniqKey="Wang H" first="Haolu" last="Wang">Haolu Wang</name><affiliation><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Liang, Xiaowen" sort="Liang, Xiaowen" uniqKey="Liang X" first="Xiaowen" last="Liang">Xiaowen Liang</name><affiliation><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Gravot, Germain" sort="Gravot, Germain" uniqKey="Gravot G" first="Germain" last="Gravot">Germain Gravot</name><affiliation><nlm:affiliation>Department of Pharmacy, University of Rennes 1, Ille-et-Vilaine, Rennes, 35043, France.</nlm:affiliation></affiliation></author><author><name sortKey="Thorling, Camilla A" sort="Thorling, Camilla A" uniqKey="Thorling C" first="Camilla A" last="Thorling">Camilla A. Thorling</name><affiliation><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Crawford, Darrell H G" sort="Crawford, Darrell H G" uniqKey="Crawford D" first="Darrell H G" last="Crawford">Darrell H G. Crawford</name><affiliation><nlm:affiliation>School of Medicine, The University of Queensland, Gallipoli Medical Research Foundation, Greenslopes Private Hospital, Greenslopes, QLD 4120, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Zhi Ping" sort="Xu, Zhi Ping" uniqKey="Xu Z" first="Zhi Ping" last="Xu">Zhi Ping Xu</name><affiliation><nlm:affiliation>Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Xin" sort="Liu, Xin" uniqKey="Liu X" first="Xin" last="Liu">Xin Liu</name><affiliation><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Roberts, Michael S" sort="Roberts, Michael S" uniqKey="Roberts M" first="Michael S" last="Roberts">Michael S. Roberts</name><affiliation><nlm:affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of biophotonics</title><idno type="eISSN">1864-0648</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity, which helps us to better understand the cellular morphology, microenvironment, immune responses and spatiotemporal dynamics of drugs and therapeutic cells in the healthy and diseased liver. This review summarizes the principles, opportunities, applications and limitations of MPM in hepatology. A key emphasis is on the use of fluorescence lifetime imaging (FLIM) to add additional quantification and specificity to the detection of endogenous fluorescent species in the liver as well as exogenous molecules and nanoparticles that are applied to the liver in vivo. We anticipate that in the near future MPM-FLIM will advance our understanding of the cellular and molecular mechanisms of liver diseases, and will be evaluated from bench to bedside, leading to real-time histology of human liver diseases.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">27312349</PMID><DateCreated><Year>2016</Year><Month>06</Month><Day>17</Day></DateCreated><DateRevised><Year>2017</Year><Month>01</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1864-0648</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>1</Issue><PubDate><Year>2017</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of biophotonics</Title><ISOAbbreviation>J Biophotonics</ISOAbbreviation></Journal><ArticleTitle>Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy.</ArticleTitle><Pagination><MedlinePgn>46-60</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/jbio.201600083</ELocationID><Abstract><AbstractText>Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity, which helps us to better understand the cellular morphology, microenvironment, immune responses and spatiotemporal dynamics of drugs and therapeutic cells in the healthy and diseased liver. This review summarizes the principles, opportunities, applications and limitations of MPM in hepatology. A key emphasis is on the use of fluorescence lifetime imaging (FLIM) to add additional quantification and specificity to the detection of endogenous fluorescent species in the liver as well as exogenous molecules and nanoparticles that are applied to the liver in vivo. We anticipate that in the near future MPM-FLIM will advance our understanding of the cellular and molecular mechanisms of liver diseases, and will be evaluated from bench to bedside, leading to real-time histology of human liver diseases.</AbstractText><CopyrightInformation>© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Haolu</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Xiaowen</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gravot</LastName><ForeName>Germain</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Pharmacy, University of Rennes 1, Ille-et-Vilaine, Rennes, 35043, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thorling</LastName><ForeName>Camilla A</ForeName><Initials>CA</Initials><AffiliationInfo><Affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Crawford</LastName><ForeName>Darrell H G</ForeName><Initials>DH</Initials><AffiliationInfo><Affiliation>School of Medicine, The University of Queensland, Gallipoli Medical Research Foundation, Greenslopes Private Hospital, Greenslopes, QLD 4120, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Zhi Ping</ForeName><Initials>ZP</Initials><AffiliationInfo><Affiliation>Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Roberts</LastName><ForeName>Michael S</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Pharmacy and Medical Science, University of South Australia, Adelaide, SA 5001, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>06</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Biophotonics</MedlineTA><NlmUniqueID>101318567</NlmUniqueID><ISSNLinking>1864-063X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Multiphoton microscopy</Keyword><Keyword MajorTopicYN="N">diseases</Keyword><Keyword MajorTopicYN="N">fluorescence lifetime imaging</Keyword><Keyword MajorTopicYN="N">function</Keyword><Keyword MajorTopicYN="N">liver</Keyword><Keyword MajorTopicYN="N">morphology</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>04</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>05</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>6</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>6</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>6</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27312349</ArticleId><ArticleId IdType="doi">10.1002/jbio.201600083</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001385 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 001385 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:27312349
|texte= Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:27312349" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |