CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity
Identifieur interne : 001936 ( Istex/Curation ); précédent : 001935; suivant : 001937CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity
Auteurs : Rose Boutros [Australie, France] ; Corinne Lorenzo [France] ; Odile Mondesert [France] ; Alain Jauneau [France] ; Vanessa Oakes [Australie] ; Christine Dozier [France] ; Brian Gabrielli [Australie] ; Bernard Ducommun [France]Source :
- Biology of the Cell [ 0248-4900 ] ; 2011-02.
English descriptors
- KwdEn :
- Association figure, Authors journal compilation, Biol, Bornens, Boutros, Brian gabrielli, Catalytic activity, Cell biol, Cell cycle, Cell division cycle, Centrin, Centriole, Centriole assembly, Centriole biogenesis, Centriole duplication, Centrosomal, Centrosomal ctn2, Centrosomal levels, Centrosomal localization, Centrosomal pool, Centrosome, Centrosome duplication, Centrosome integrity, Centrosome levels, Chk1, Christine dozier, Corinne lorenzo, Ctn1, Ctn2, Ctn2 sirna duplexes, Ctn3, Ctns, Curie institute, Ducommun, Duplex, Endogenous, Endogenous ctn2, Flim, Fusion proteins, Gabrielli, Hela, Hela cells, Human cells, Human centrin, Imaging, Independent experiments, Localization, Lysis buffer, Mammalian cells, Michel bornens, Microtubule, Morange, Mother centriole, Multiple centrosome proteins, Mutant, Nitrocellulose membranes, Nucleotide excision repair, Odile mondesert, Others figure, Overall integrity, Phosphatase, Portland press, Present study, Protease inhibitors, Protein complexes, Protein extracts, Research article, Santa cruz biotechnology, Sirna, Sirna duplexes, Streak camera, Supplementary figure, Toulouse, Tubulin, U2os, U2os cells, Uorescence, Uorescence lifetime, Uorescence lifetime imaging microscopy, Xeroderma pigmentosum group.
- Teeft :
- Association figure, Authors journal compilation, Biol, Bornens, Boutros, Brian gabrielli, Catalytic activity, Cell biol, Cell cycle, Cell division cycle, Centrin, Centriole, Centriole assembly, Centriole biogenesis, Centriole duplication, Centrosomal, Centrosomal ctn2, Centrosomal levels, Centrosomal localization, Centrosomal pool, Centrosome, Centrosome duplication, Centrosome integrity, Centrosome levels, Chk1, Christine dozier, Corinne lorenzo, Ctn1, Ctn2, Ctn2 sirna duplexes, Ctn3, Ctns, Curie institute, Ducommun, Duplex, Endogenous, Endogenous ctn2, Flim, Fusion proteins, Gabrielli, Hela, Hela cells, Human cells, Human centrin, Imaging, Independent experiments, Localization, Lysis buffer, Mammalian cells, Michel bornens, Microtubule, Morange, Mother centriole, Multiple centrosome proteins, Mutant, Nitrocellulose membranes, Nucleotide excision repair, Odile mondesert, Others figure, Overall integrity, Phosphatase, Portland press, Present study, Protease inhibitors, Protein complexes, Protein extracts, Research article, Santa cruz biotechnology, Sirna, Sirna duplexes, Streak camera, Supplementary figure, Toulouse, Tubulin, U2os, U2os cells, Uorescence, Uorescence lifetime, Uorescence lifetime imaging microscopy, Xeroderma pigmentosum group.
Abstract
Background information. CDC25 (cell division cycle 25) phosphatases function as activators of CDK (cyclin‐dependent kinase)–cyclin complexes to regulate progression through the CDC. We have recently identified a pool of CDC25B at the centrosome of interphase cells that plays a role in regulating centrosome numbers. Results. In the present study, we demonstrate that CDC25B forms a close association with Ctn (centrin) proteins at the centrosome. This interaction involves both N‐ and C‐terminal regions of CDC25B and requires CDC25B binding to its CDK—cyclin substrates. However, the interaction is not dependent on the enzyme activity of CDC25B. Although CDC25B appears to bind indirectly to Ctn2, this association is pertinent to CDC25B localization at the centrosome. We further demonstrate that CDC25B plays a role in maintaining the overall integrity of the centrosome, by regulating the centrosome levels of multiple centrosome proteins, including that of Ctn2. Conclusions. Our results therefore suggest that CDC25B associates with a Ctn2‐containing multiprotein complex in the cytoplasm, which targets it to the centrosome, where it plays a role in maintaining the centrosome levels of Ctn2 and a number of other centrosome components.
Url:
DOI: 10.1042/BC20100111
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001936
Links to Exploration step
ISTEX:862895CE331F3CAEAE90459EA3274674D0D5D888Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity</title>
<author><name sortKey="Boutros, Rose" sort="Boutros, Rose" uniqKey="Boutros R" first="Rose" last="Boutros">Rose Boutros</name>
<affiliation wicri:level="1"><mods:affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</mods:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>E-mail: bernard.ducommun@itav‐recherche.fr</mods:affiliation>
<country wicri:rule="url">France</country>
</affiliation>
</author>
<author><name sortKey="Lorenzo, Corinne" sort="Lorenzo, Corinne" uniqKey="Lorenzo C" first="Corinne" last="Lorenzo">Corinne Lorenzo</name>
<affiliation wicri:level="1"><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mondesert, Odile" sort="Mondesert, Odile" uniqKey="Mondesert O" first="Odile" last="Mondesert">Odile Mondesert</name>
<affiliation wicri:level="1"><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Jauneau, Alain" sort="Jauneau, Alain" uniqKey="Jauneau A" first="Alain" last="Jauneau">Alain Jauneau</name>
<affiliation wicri:level="1"><mods:affiliation>CNRS, Plateforme Imagerie, Pôle de Biotechnologie Végétale, F‐31320 Castanet Tolosan, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>CNRS, Plateforme Imagerie, Pôle de Biotechnologie Végétale, F‐31320 Castanet Tolosan</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Oakes, Vanessa" sort="Oakes, Vanessa" uniqKey="Oakes V" first="Vanessa" last="Oakes">Vanessa Oakes</name>
<affiliation wicri:level="1"><mods:affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</mods:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dozier, Christine" sort="Dozier, Christine" uniqKey="Dozier C" first="Christine" last="Dozier">Christine Dozier</name>
<affiliation wicri:level="1"><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Gabrielli, Brian" sort="Gabrielli, Brian" uniqKey="Gabrielli B" first="Brian" last="Gabrielli">Brian Gabrielli</name>
<affiliation wicri:level="1"><mods:affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</mods:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ducommun, Bernard" sort="Ducommun, Bernard" uniqKey="Ducommun B" first="Bernard" last="Ducommun">Bernard Ducommun</name>
<affiliation wicri:level="1"><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>CHU Purpan, Service d'Hématologie, TSA 40031, F‐31059 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>CHU Purpan, Service d'Hématologie, TSA 40031, F‐31059 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>E-mail: bernard.ducommun@itav‐recherche.fr</mods:affiliation>
<country wicri:rule="url">France</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:862895CE331F3CAEAE90459EA3274674D0D5D888</idno>
<date when="2011" year="2011">2011</date>
<idno type="doi">10.1042/BC20100111</idno>
<idno type="url">https://api.istex.fr/document/862895CE331F3CAEAE90459EA3274674D0D5D888/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001936</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001936</idno>
<idno type="wicri:Area/Istex/Curation">001936</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity</title>
<author><name sortKey="Boutros, Rose" sort="Boutros, Rose" uniqKey="Boutros R" first="Rose" last="Boutros">Rose Boutros</name>
<affiliation wicri:level="1"><mods:affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</mods:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>E-mail: bernard.ducommun@itav‐recherche.fr</mods:affiliation>
<country wicri:rule="url">France</country>
</affiliation>
</author>
<author><name sortKey="Lorenzo, Corinne" sort="Lorenzo, Corinne" uniqKey="Lorenzo C" first="Corinne" last="Lorenzo">Corinne Lorenzo</name>
<affiliation wicri:level="1"><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mondesert, Odile" sort="Mondesert, Odile" uniqKey="Mondesert O" first="Odile" last="Mondesert">Odile Mondesert</name>
<affiliation wicri:level="1"><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Jauneau, Alain" sort="Jauneau, Alain" uniqKey="Jauneau A" first="Alain" last="Jauneau">Alain Jauneau</name>
<affiliation wicri:level="1"><mods:affiliation>CNRS, Plateforme Imagerie, Pôle de Biotechnologie Végétale, F‐31320 Castanet Tolosan, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>CNRS, Plateforme Imagerie, Pôle de Biotechnologie Végétale, F‐31320 Castanet Tolosan</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Oakes, Vanessa" sort="Oakes, Vanessa" uniqKey="Oakes V" first="Vanessa" last="Oakes">Vanessa Oakes</name>
<affiliation wicri:level="1"><mods:affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</mods:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dozier, Christine" sort="Dozier, Christine" uniqKey="Dozier C" first="Christine" last="Dozier">Christine Dozier</name>
<affiliation wicri:level="1"><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Gabrielli, Brian" sort="Gabrielli, Brian" uniqKey="Gabrielli B" first="Brian" last="Gabrielli">Brian Gabrielli</name>
<affiliation wicri:level="1"><mods:affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</mods:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ducommun, Bernard" sort="Ducommun, Bernard" uniqKey="Ducommun B" first="Bernard" last="Ducommun">Bernard Ducommun</name>
<affiliation wicri:level="1"><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>CHU Purpan, Service d'Hématologie, TSA 40031, F‐31059 Toulouse, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>CHU Purpan, Service d'Hématologie, TSA 40031, F‐31059 Toulouse</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>E-mail: bernard.ducommun@itav‐recherche.fr</mods:affiliation>
<country wicri:rule="url">France</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Biology of the Cell</title>
<title level="j" type="alt">BIOLOGY OF THE CELL</title>
<idno type="ISSN">0248-4900</idno>
<idno type="eISSN">1768-322X</idno>
<imprint><biblScope unit="vol">103</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="55">55</biblScope>
<biblScope unit="page" to="68">68</biblScope>
<biblScope unit="page-count">14</biblScope>
<publisher>Blackwell Publishing Ltd</publisher>
<pubPlace>Oxford, UK</pubPlace>
<date type="published" when="2011-02">2011-02</date>
</imprint>
<idno type="ISSN">0248-4900</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0248-4900</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Association figure</term>
<term>Authors journal compilation</term>
<term>Biol</term>
<term>Bornens</term>
<term>Boutros</term>
<term>Brian gabrielli</term>
<term>Catalytic activity</term>
<term>Cell biol</term>
<term>Cell cycle</term>
<term>Cell division cycle</term>
<term>Centrin</term>
<term>Centriole</term>
<term>Centriole assembly</term>
<term>Centriole biogenesis</term>
<term>Centriole duplication</term>
<term>Centrosomal</term>
<term>Centrosomal ctn2</term>
<term>Centrosomal levels</term>
<term>Centrosomal localization</term>
<term>Centrosomal pool</term>
<term>Centrosome</term>
<term>Centrosome duplication</term>
<term>Centrosome integrity</term>
<term>Centrosome levels</term>
<term>Chk1</term>
<term>Christine dozier</term>
<term>Corinne lorenzo</term>
<term>Ctn1</term>
<term>Ctn2</term>
<term>Ctn2 sirna duplexes</term>
<term>Ctn3</term>
<term>Ctns</term>
<term>Curie institute</term>
<term>Ducommun</term>
<term>Duplex</term>
<term>Endogenous</term>
<term>Endogenous ctn2</term>
<term>Flim</term>
<term>Fusion proteins</term>
<term>Gabrielli</term>
<term>Hela</term>
<term>Hela cells</term>
<term>Human cells</term>
<term>Human centrin</term>
<term>Imaging</term>
<term>Independent experiments</term>
<term>Localization</term>
<term>Lysis buffer</term>
<term>Mammalian cells</term>
<term>Michel bornens</term>
<term>Microtubule</term>
<term>Morange</term>
<term>Mother centriole</term>
<term>Multiple centrosome proteins</term>
<term>Mutant</term>
<term>Nitrocellulose membranes</term>
<term>Nucleotide excision repair</term>
<term>Odile mondesert</term>
<term>Others figure</term>
<term>Overall integrity</term>
<term>Phosphatase</term>
<term>Portland press</term>
<term>Present study</term>
<term>Protease inhibitors</term>
<term>Protein complexes</term>
<term>Protein extracts</term>
<term>Research article</term>
<term>Santa cruz biotechnology</term>
<term>Sirna</term>
<term>Sirna duplexes</term>
<term>Streak camera</term>
<term>Supplementary figure</term>
<term>Toulouse</term>
<term>Tubulin</term>
<term>U2os</term>
<term>U2os cells</term>
<term>Uorescence</term>
<term>Uorescence lifetime</term>
<term>Uorescence lifetime imaging microscopy</term>
<term>Xeroderma pigmentosum group</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Association figure</term>
<term>Authors journal compilation</term>
<term>Biol</term>
<term>Bornens</term>
<term>Boutros</term>
<term>Brian gabrielli</term>
<term>Catalytic activity</term>
<term>Cell biol</term>
<term>Cell cycle</term>
<term>Cell division cycle</term>
<term>Centrin</term>
<term>Centriole</term>
<term>Centriole assembly</term>
<term>Centriole biogenesis</term>
<term>Centriole duplication</term>
<term>Centrosomal</term>
<term>Centrosomal ctn2</term>
<term>Centrosomal levels</term>
<term>Centrosomal localization</term>
<term>Centrosomal pool</term>
<term>Centrosome</term>
<term>Centrosome duplication</term>
<term>Centrosome integrity</term>
<term>Centrosome levels</term>
<term>Chk1</term>
<term>Christine dozier</term>
<term>Corinne lorenzo</term>
<term>Ctn1</term>
<term>Ctn2</term>
<term>Ctn2 sirna duplexes</term>
<term>Ctn3</term>
<term>Ctns</term>
<term>Curie institute</term>
<term>Ducommun</term>
<term>Duplex</term>
<term>Endogenous</term>
<term>Endogenous ctn2</term>
<term>Flim</term>
<term>Fusion proteins</term>
<term>Gabrielli</term>
<term>Hela</term>
<term>Hela cells</term>
<term>Human cells</term>
<term>Human centrin</term>
<term>Imaging</term>
<term>Independent experiments</term>
<term>Localization</term>
<term>Lysis buffer</term>
<term>Mammalian cells</term>
<term>Michel bornens</term>
<term>Microtubule</term>
<term>Morange</term>
<term>Mother centriole</term>
<term>Multiple centrosome proteins</term>
<term>Mutant</term>
<term>Nitrocellulose membranes</term>
<term>Nucleotide excision repair</term>
<term>Odile mondesert</term>
<term>Others figure</term>
<term>Overall integrity</term>
<term>Phosphatase</term>
<term>Portland press</term>
<term>Present study</term>
<term>Protease inhibitors</term>
<term>Protein complexes</term>
<term>Protein extracts</term>
<term>Research article</term>
<term>Santa cruz biotechnology</term>
<term>Sirna</term>
<term>Sirna duplexes</term>
<term>Streak camera</term>
<term>Supplementary figure</term>
<term>Toulouse</term>
<term>Tubulin</term>
<term>U2os</term>
<term>U2os cells</term>
<term>Uorescence</term>
<term>Uorescence lifetime</term>
<term>Uorescence lifetime imaging microscopy</term>
<term>Xeroderma pigmentosum group</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Background information. CDC25 (cell division cycle 25) phosphatases function as activators of CDK (cyclin‐dependent kinase)–cyclin complexes to regulate progression through the CDC. We have recently identified a pool of CDC25B at the centrosome of interphase cells that plays a role in regulating centrosome numbers. Results. In the present study, we demonstrate that CDC25B forms a close association with Ctn (centrin) proteins at the centrosome. This interaction involves both N‐ and C‐terminal regions of CDC25B and requires CDC25B binding to its CDK—cyclin substrates. However, the interaction is not dependent on the enzyme activity of CDC25B. Although CDC25B appears to bind indirectly to Ctn2, this association is pertinent to CDC25B localization at the centrosome. We further demonstrate that CDC25B plays a role in maintaining the overall integrity of the centrosome, by regulating the centrosome levels of multiple centrosome proteins, including that of Ctn2. Conclusions. Our results therefore suggest that CDC25B associates with a Ctn2‐containing multiprotein complex in the cytoplasm, which targets it to the centrosome, where it plays a role in maintaining the centrosome levels of Ctn2 and a number of other centrosome components.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001936 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 001936 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:862895CE331F3CAEAE90459EA3274674D0D5D888 |texte= CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |