CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity
Identifieur interne : 001936 ( Istex/Corpus ); précédent : 001935; suivant : 001937CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity
Auteurs : Rose Boutros ; Corinne Lorenzo ; Odile Mondesert ; Alain Jauneau ; Vanessa Oakes ; Christine Dozier ; Brian Gabrielli ; Bernard DucommunSource :
- Biology of the Cell [ 0248-4900 ] ; 2011-02.
English descriptors
- KwdEn :
- Association figure, Authors journal compilation, Biol, Bornens, Boutros, Brian gabrielli, Catalytic activity, Cell biol, Cell cycle, Cell division cycle, Centrin, Centriole, Centriole assembly, Centriole biogenesis, Centriole duplication, Centrosomal, Centrosomal ctn2, Centrosomal levels, Centrosomal localization, Centrosomal pool, Centrosome, Centrosome duplication, Centrosome integrity, Centrosome levels, Chk1, Christine dozier, Corinne lorenzo, Ctn1, Ctn2, Ctn2 sirna duplexes, Ctn3, Ctns, Curie institute, Ducommun, Duplex, Endogenous, Endogenous ctn2, Flim, Fusion proteins, Gabrielli, Hela, Hela cells, Human cells, Human centrin, Imaging, Independent experiments, Localization, Lysis buffer, Mammalian cells, Michel bornens, Microtubule, Morange, Mother centriole, Multiple centrosome proteins, Mutant, Nitrocellulose membranes, Nucleotide excision repair, Odile mondesert, Others figure, Overall integrity, Phosphatase, Portland press, Present study, Protease inhibitors, Protein complexes, Protein extracts, Research article, Santa cruz biotechnology, Sirna, Sirna duplexes, Streak camera, Supplementary figure, Toulouse, Tubulin, U2os, U2os cells, Uorescence, Uorescence lifetime, Uorescence lifetime imaging microscopy, Xeroderma pigmentosum group.
- Teeft :
- Association figure, Authors journal compilation, Biol, Bornens, Boutros, Brian gabrielli, Catalytic activity, Cell biol, Cell cycle, Cell division cycle, Centrin, Centriole, Centriole assembly, Centriole biogenesis, Centriole duplication, Centrosomal, Centrosomal ctn2, Centrosomal levels, Centrosomal localization, Centrosomal pool, Centrosome, Centrosome duplication, Centrosome integrity, Centrosome levels, Chk1, Christine dozier, Corinne lorenzo, Ctn1, Ctn2, Ctn2 sirna duplexes, Ctn3, Ctns, Curie institute, Ducommun, Duplex, Endogenous, Endogenous ctn2, Flim, Fusion proteins, Gabrielli, Hela, Hela cells, Human cells, Human centrin, Imaging, Independent experiments, Localization, Lysis buffer, Mammalian cells, Michel bornens, Microtubule, Morange, Mother centriole, Multiple centrosome proteins, Mutant, Nitrocellulose membranes, Nucleotide excision repair, Odile mondesert, Others figure, Overall integrity, Phosphatase, Portland press, Present study, Protease inhibitors, Protein complexes, Protein extracts, Research article, Santa cruz biotechnology, Sirna, Sirna duplexes, Streak camera, Supplementary figure, Toulouse, Tubulin, U2os, U2os cells, Uorescence, Uorescence lifetime, Uorescence lifetime imaging microscopy, Xeroderma pigmentosum group.
Abstract
Background information. CDC25 (cell division cycle 25) phosphatases function as activators of CDK (cyclin‐dependent kinase)–cyclin complexes to regulate progression through the CDC. We have recently identified a pool of CDC25B at the centrosome of interphase cells that plays a role in regulating centrosome numbers. Results. In the present study, we demonstrate that CDC25B forms a close association with Ctn (centrin) proteins at the centrosome. This interaction involves both N‐ and C‐terminal regions of CDC25B and requires CDC25B binding to its CDK—cyclin substrates. However, the interaction is not dependent on the enzyme activity of CDC25B. Although CDC25B appears to bind indirectly to Ctn2, this association is pertinent to CDC25B localization at the centrosome. We further demonstrate that CDC25B plays a role in maintaining the overall integrity of the centrosome, by regulating the centrosome levels of multiple centrosome proteins, including that of Ctn2. Conclusions. Our results therefore suggest that CDC25B associates with a Ctn2‐containing multiprotein complex in the cytoplasm, which targets it to the centrosome, where it plays a role in maintaining the centrosome levels of Ctn2 and a number of other centrosome components.
Url:
DOI: 10.1042/BC20100111
Links to Exploration step
ISTEX:862895CE331F3CAEAE90459EA3274674D0D5D888Le document en format XML
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bernard.ducommun@itav‐recherche.fr</mods:affiliation></affiliation></author><author><name sortKey="Lorenzo, Corinne" sort="Lorenzo, Corinne" uniqKey="Lorenzo C" first="Corinne" last="Lorenzo">Corinne Lorenzo</name><affiliation><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation></affiliation></author><author><name sortKey="Mondesert, Odile" sort="Mondesert, Odile" uniqKey="Mondesert O" first="Odile" last="Mondesert">Odile Mondesert</name><affiliation><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation></affiliation></author><author><name sortKey="Jauneau, Alain" sort="Jauneau, Alain" uniqKey="Jauneau A" first="Alain" last="Jauneau">Alain Jauneau</name><affiliation><mods:affiliation>CNRS, Plateforme Imagerie, Pôle de Biotechnologie Végétale, F‐31320 Castanet Tolosan, France</mods:affiliation></affiliation></author><author><name sortKey="Oakes, Vanessa" sort="Oakes, Vanessa" uniqKey="Oakes V" first="Vanessa" last="Oakes">Vanessa Oakes</name><affiliation><mods:affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</mods:affiliation></affiliation></author><author><name sortKey="Dozier, Christine" sort="Dozier, Christine" uniqKey="Dozier C" first="Christine" last="Dozier">Christine Dozier</name><affiliation><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation></affiliation></author><author><name sortKey="Gabrielli, Brian" sort="Gabrielli, Brian" uniqKey="Gabrielli B" first="Brian" last="Gabrielli">Brian Gabrielli</name><affiliation><mods:affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</mods:affiliation></affiliation></author><author><name sortKey="Ducommun, Bernard" sort="Ducommun, Bernard" uniqKey="Ducommun B" first="Bernard" last="Ducommun">Bernard Ducommun</name><affiliation><mods:affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</mods:affiliation></affiliation><affiliation><mods:affiliation>CHU Purpan, Service d'Hématologie, TSA 40031, F‐31059 Toulouse, France</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: bernard.ducommun@itav‐recherche.fr</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biology of the Cell</title><title level="j" type="alt">BIOLOGY OF THE CELL</title><idno type="ISSN">0248-4900</idno><idno type="eISSN">1768-322X</idno><imprint><biblScope unit="vol">103</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="55">55</biblScope><biblScope unit="page" to="68">68</biblScope><biblScope unit="page-count">14</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2011-02">2011-02</date></imprint><idno type="ISSN">0248-4900</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0248-4900</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Association figure</term><term>Authors journal compilation</term><term>Biol</term><term>Bornens</term><term>Boutros</term><term>Brian gabrielli</term><term>Catalytic activity</term><term>Cell biol</term><term>Cell cycle</term><term>Cell division cycle</term><term>Centrin</term><term>Centriole</term><term>Centriole assembly</term><term>Centriole biogenesis</term><term>Centriole duplication</term><term>Centrosomal</term><term>Centrosomal ctn2</term><term>Centrosomal levels</term><term>Centrosomal localization</term><term>Centrosomal pool</term><term>Centrosome</term><term>Centrosome duplication</term><term>Centrosome integrity</term><term>Centrosome levels</term><term>Chk1</term><term>Christine dozier</term><term>Corinne lorenzo</term><term>Ctn1</term><term>Ctn2</term><term>Ctn2 sirna duplexes</term><term>Ctn3</term><term>Ctns</term><term>Curie institute</term><term>Ducommun</term><term>Duplex</term><term>Endogenous</term><term>Endogenous ctn2</term><term>Flim</term><term>Fusion proteins</term><term>Gabrielli</term><term>Hela</term><term>Hela cells</term><term>Human cells</term><term>Human centrin</term><term>Imaging</term><term>Independent experiments</term><term>Localization</term><term>Lysis buffer</term><term>Mammalian cells</term><term>Michel bornens</term><term>Microtubule</term><term>Morange</term><term>Mother centriole</term><term>Multiple centrosome proteins</term><term>Mutant</term><term>Nitrocellulose membranes</term><term>Nucleotide excision repair</term><term>Odile mondesert</term><term>Others figure</term><term>Overall integrity</term><term>Phosphatase</term><term>Portland press</term><term>Present study</term><term>Protease inhibitors</term><term>Protein complexes</term><term>Protein extracts</term><term>Research article</term><term>Santa cruz biotechnology</term><term>Sirna</term><term>Sirna duplexes</term><term>Streak camera</term><term>Supplementary figure</term><term>Toulouse</term><term>Tubulin</term><term>U2os</term><term>U2os cells</term><term>Uorescence</term><term>Uorescence lifetime</term><term>Uorescence lifetime imaging microscopy</term><term>Xeroderma pigmentosum group</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Association figure</term><term>Authors journal compilation</term><term>Biol</term><term>Bornens</term><term>Boutros</term><term>Brian gabrielli</term><term>Catalytic activity</term><term>Cell biol</term><term>Cell cycle</term><term>Cell division cycle</term><term>Centrin</term><term>Centriole</term><term>Centriole assembly</term><term>Centriole biogenesis</term><term>Centriole duplication</term><term>Centrosomal</term><term>Centrosomal ctn2</term><term>Centrosomal levels</term><term>Centrosomal localization</term><term>Centrosomal pool</term><term>Centrosome</term><term>Centrosome duplication</term><term>Centrosome integrity</term><term>Centrosome levels</term><term>Chk1</term><term>Christine dozier</term><term>Corinne lorenzo</term><term>Ctn1</term><term>Ctn2</term><term>Ctn2 sirna duplexes</term><term>Ctn3</term><term>Ctns</term><term>Curie institute</term><term>Ducommun</term><term>Duplex</term><term>Endogenous</term><term>Endogenous ctn2</term><term>Flim</term><term>Fusion proteins</term><term>Gabrielli</term><term>Hela</term><term>Hela cells</term><term>Human cells</term><term>Human centrin</term><term>Imaging</term><term>Independent experiments</term><term>Localization</term><term>Lysis buffer</term><term>Mammalian cells</term><term>Michel bornens</term><term>Microtubule</term><term>Morange</term><term>Mother centriole</term><term>Multiple centrosome proteins</term><term>Mutant</term><term>Nitrocellulose membranes</term><term>Nucleotide excision repair</term><term>Odile mondesert</term><term>Others figure</term><term>Overall integrity</term><term>Phosphatase</term><term>Portland press</term><term>Present study</term><term>Protease inhibitors</term><term>Protein complexes</term><term>Protein extracts</term><term>Research article</term><term>Santa cruz biotechnology</term><term>Sirna</term><term>Sirna duplexes</term><term>Streak camera</term><term>Supplementary figure</term><term>Toulouse</term><term>Tubulin</term><term>U2os</term><term>U2os cells</term><term>Uorescence</term><term>Uorescence lifetime</term><term>Uorescence lifetime imaging microscopy</term><term>Xeroderma pigmentosum group</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background information. CDC25 (cell division cycle 25) phosphatases function as activators of CDK (cyclin‐dependent kinase)–cyclin complexes to regulate progression through the CDC. We have recently identified a pool of CDC25B at the centrosome of interphase cells that plays a role in regulating centrosome numbers. Results. In the present study, we demonstrate that CDC25B forms a close association with Ctn (centrin) proteins at the centrosome. This interaction involves both N‐ and C‐terminal regions of CDC25B and requires CDC25B binding to its CDK—cyclin substrates. However, the interaction is not dependent on the enzyme activity of CDC25B. Although CDC25B appears to bind indirectly to Ctn2, this association is pertinent to CDC25B localization at the centrosome. We further demonstrate that CDC25B plays a role in maintaining the overall integrity of the centrosome, by regulating the centrosome levels of multiple centrosome proteins, including that of Ctn2. Conclusions. Our results therefore suggest that CDC25B associates with a Ctn2‐containing multiprotein complex in the cytoplasm, which targets it to the centrosome, where it plays a role in maintaining the centrosome levels of Ctn2 and a number of other centrosome components.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>centrosome</json:string><json:string>ctn2</json:string><json:string>centriole</json:string><json:string>centrosomal</json:string><json:string>boutros</json:string><json:string>hela</json:string><json:string>biol</json:string><json:string>sirna</json:string><json:string>uorescence</json:string><json:string>centrin</json:string><json:string>u2os</json:string><json:string>ctn1</json:string><json:string>tubulin</json:string><json:string>mutant</json:string><json:string>gabrielli</json:string><json:string>ducommun</json:string><json:string>chk1</json:string><json:string>authors journal compilation</json:string><json:string>phosphatase</json:string><json:string>portland press</json:string><json:string>microtubule</json:string><json:string>bornens</json:string><json:string>centriole duplication</json:string><json:string>morange</json:string><json:string>ctn3</json:string><json:string>cell cycle</json:string><json:string>hela cells</json:string><json:string>flim</json:string><json:string>supplementary figure</json:string><json:string>ctns</json:string><json:string>u2os cells</json:string><json:string>fusion proteins</json:string><json:string>centrosome duplication</json:string><json:string>others figure</json:string><json:string>independent experiments</json:string><json:string>cell biol</json:string><json:string>toulouse</json:string><json:string>duplex</json:string><json:string>centriole assembly</json:string><json:string>mammalian cells</json:string><json:string>uorescence lifetime imaging microscopy</json:string><json:string>ctn2 sirna duplexes</json:string><json:string>human cells</json:string><json:string>michel bornens</json:string><json:string>corinne lorenzo</json:string><json:string>mother centriole</json:string><json:string>research article</json:string><json:string>centrosomal pool</json:string><json:string>sirna duplexes</json:string><json:string>curie institute</json:string><json:string>centrosome integrity</json:string><json:string>centrosomal ctn2</json:string><json:string>association figure</json:string><json:string>multiple centrosome proteins</json:string><json:string>odile mondesert</json:string><json:string>endogenous</json:string><json:string>localization</json:string><json:string>christine dozier</json:string><json:string>present study</json:string><json:string>nucleotide excision repair</json:string><json:string>protein complexes</json:string><json:string>centriole biogenesis</json:string><json:string>overall integrity</json:string><json:string>cell division cycle</json:string><json:string>uorescence lifetime</json:string><json:string>centrosome levels</json:string><json:string>endogenous ctn2</json:string><json:string>centrosomal localization</json:string><json:string>protein extracts</json:string><json:string>centrosomal levels</json:string><json:string>brian gabrielli</json:string><json:string>catalytic activity</json:string><json:string>santa cruz biotechnology</json:string><json:string>streak camera</json:string><json:string>protease inhibitors</json:string><json:string>lysis buffer</json:string><json:string>nitrocellulose membranes</json:string><json:string>xeroderma pigmentosum group</json:string><json:string>human centrin</json:string><json:string>imaging</json:string></teeft></keywords><author><json:item><name>Rose Boutros</name><affiliations><json:string>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</json:string><json:string>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</json:string><json:string>E-mail: bernard.ducommun@itav‐recherche.fr</json:string></affiliations></json:item><json:item><name>Corinne Lorenzo</name><affiliations><json:string>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</json:string><json:string>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</json:string></affiliations></json:item><json:item><name>Odile Mondesert</name><affiliations><json:string>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</json:string><json:string>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</json:string></affiliations></json:item><json:item><name>Alain Jauneau</name><affiliations><json:string>CNRS, Plateforme Imagerie, Pôle de Biotechnologie Végétale, F‐31320 Castanet Tolosan, France</json:string></affiliations></json:item><json:item><name>Vanessa Oakes</name><affiliations><json:string>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</json:string></affiliations></json:item><json:item><name>Christine Dozier</name><affiliations><json:string>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</json:string><json:string>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</json:string></affiliations></json:item><json:item><name>Brian Gabrielli</name><affiliations><json:string>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</json:string></affiliations></json:item><json:item><name>Bernard Ducommun</name><affiliations><json:string>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</json:string><json:string>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</json:string><json:string>CHU Purpan, Service d'Hématologie, TSA 40031, F‐31059 Toulouse, France</json:string><json:string>E-mail: bernard.ducommun@itav‐recherche.fr</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>CDC25B</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>centrin (Ctn)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>centrosome</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cyclin‐dependent kinase (CDK)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>fluorescence lifetime imaging microscopy (FLIM)</value></json:item></subject><articleId><json:string>BOC80</json:string></articleId><arkIstex>ark:/67375/WNG-QNVBG7DH-N</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Background information. CDC25 (cell division cycle 25) phosphatases function as activators of CDK (cyclin‐dependent kinase)–cyclin complexes to regulate progression through the CDC. We have recently identified a pool of CDC25B at the centrosome of interphase cells that plays a role in regulating centrosome numbers. Results. In the present study, we demonstrate that CDC25B forms a close association with Ctn (centrin) proteins at the centrosome. This interaction involves both N‐ and C‐terminal regions of CDC25B and requires CDC25B binding to its CDK—cyclin substrates. However, the interaction is not dependent on the enzyme activity of CDC25B. Although CDC25B appears to bind indirectly to Ctn2, this association is pertinent to CDC25B localization at the centrosome. We further demonstrate that CDC25B plays a role in maintaining the overall integrity of the centrosome, by regulating the centrosome levels of multiple centrosome proteins, including that of Ctn2. Conclusions. Our results therefore suggest that CDC25B associates with a Ctn2‐containing multiprotein complex in the cytoplasm, which targets it to the centrosome, where it plays a role in maintaining the centrosome levels of Ctn2 and a number of other centrosome components.</abstract><qualityIndicators><score>9.184</score><pdfWordCount>7541</pdfWordCount><pdfCharCount>48744</pdfCharCount><pdfVersion>1.6</pdfVersion><pdfPageCount>21</pdfPageCount><pdfPageSize>595.276 x 788.031 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>182</abstractWordCount><abstractCharCount>1245</abstractCharCount><keywordCount>5</keywordCount></qualityIndicators><title>CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity</title><pmid><json:string>21091437</json:string></pmid><genre><json:string>article</json:string></genre><host><title>Biology of the Cell</title><language><json:string>unknown</json:string></language><doi><json:string>10.1111/(ISSN)1768-322X</json:string></doi><issn><json:string>0248-4900</json:string></issn><eissn><json:string>1768-322X</json:string></eissn><publisherId><json:string>BOC</json:string></publisherId><volume>103</volume><issue>2</issue><pages><first>55</first><last>68</last><total>14</total></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>2011</json:string><json:string>4096</json:string></date><geogName></geogName><orgName><json:string>Carl Zeiss</json:string><json:string>France and Children</json:string><json:string>Santa Cruz Biotechnology</json:string><json:string>Curie Institute, Paris, France</json:string><json:string>France, CNRS, Plateforme Imagerie, Pole</json:string><json:string>Medical Research Institute, The University of Sydney</json:string><json:string>American Type Culture Collection</json:string><json:string>Polyplus-Transfection Inc.</json:string><json:string>France Background</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>C. Beads</json:string><json:string>Alexa Fluor</json:string><json:string>Vanessa Oakes</json:string><json:string>CHU Purpan</json:string><json:string>Odile Mondesert</json:string><json:string>Brian Gabrielli</json:string><json:string>Michel Bornens</json:string><json:string>Alain Jauneau</json:string><json:string>Jeffrey Salisbury</json:string><json:string>S. Miron</json:string><json:string>Corinne Lorenzo</json:string><json:string>Pierre Potier</json:string><json:string>Christine Dozier</json:string><json:string>Andreas Merdes</json:string><json:string>Bernard Ducommun</json:string></persName><placeName><json:string>Rochester</json:string><json:string>NY</json:string><json:string>Australia</json:string><json:string>Centre</json:string><json:string>Portland</json:string><json:string>Narbonne</json:string><json:string>Northampton</json:string><json:string>U.S.</json:string><json:string>Santa Cruz</json:string><json:string>Toulouse</json:string><json:string>MA</json:string><json:string>France</json:string><json:string>Hamamatsu</json:string></placeName><ref_url><json:string>http://www.biolcell.org/boc/</json:string><json:string>http://www.biolcell.org/</json:string></ref_url><ref_bibl><json:string>Koblenz et al., 2003</json:string><json:string>Rodrigues-Martins et al., 2007a, 2007b</json:string><json:string>Stemm-Wolf et al., 2005</json:string><json:string>Boutros and Ducommun, 2008</json:string><json:string>Nishi et al., 2005</json:string><json:string>Prosser et al., 2009</json:string><json:string>Kasbek et al., 2007</json:string><json:string>Meraldi et al., 1999</json:string><json:string>Rodrigues-Martins et al., 2007b</json:string><json:string>Ruiz et al., 2005</json:string><json:string>Lutz et al., 2001</json:string><json:string>Boutros et al., 2007b</json:string><json:string>Sohn et al., 2004</json:string><json:string>Forrest and Gabrielli, 2001</json:string><json:string>Azimzadeh et al., 2009</json:string><json:string>Klein and Nigg, 2009</json:string><json:string>Krishnan et al., 2003</json:string><json:string>Boutros et al., 2007a</json:string><json:string>Gabrielli et al. (1996)</json:string><json:string>Charbonnier et al., 2006</json:string><json:string>Bettencourt-Dias et al., 2005</json:string><json:string>Kilmartin, 2003</json:string><json:string>Hinchcliffe et al., 1999</json:string><json:string>Salisbury, 2007</json:string><json:string>Baron et al., 1994</json:string><json:string>Dammermann et al., 2004</json:string><json:string>Azimzadeh and Bornens, 2007</json:string><json:string>Doxsey, 2001</json:string><json:string>Kramer et al., 2004</json:string><json:string>Paoletti et al., 1996</json:string><json:string>Schmitt et al., 2006</json:string><json:string>Chen and Periasamy, 2004</json:string><json:string>Tsang et al., 2006</json:string><json:string>Araki et al., 2001</json:string><json:string>Friedberg, 2006</json:string><json:string>Dammermann and Merdes, 2002</json:string><json:string>Paoletti et al., 2003</json:string><json:string>Yang et al., 2006</json:string><json:string>Martinez-Sanz et al., 2006</json:string><json:string>Salisbury et al., 2002</json:string><json:string>Klink and Wolniak, 2001</json:string><json:string>Resendes et al., 2008</json:string><json:string>Ruiz et al., 1999</json:string><json:string>Baum et al., 1986</json:string><json:string>Chen et al., 2002</json:string><json:string>Kleylein-Sohn et al., 2007</json:string><json:string>Gabrielli et al., 1996</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-QNVBG7DH-N</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - cell biology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - developmental biology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Cell Biology</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - General Medicine</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string><json:string>4 - gastroenterologie. foie. pancreas. abdomen</json:string></inist></categories><publicationDate>2011</publicationDate><copyrightDate>2011</copyrightDate><doi><json:string>10.1042/BC20100111</json:string></doi><id>862895CE331F3CAEAE90459EA3274674D0D5D888</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/862895CE331F3CAEAE90459EA3274674D0D5D888/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/862895CE331F3CAEAE90459EA3274674D0D5D888/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/862895CE331F3CAEAE90459EA3274674D0D5D888/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main">CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><licence>2011 Société Française des Microscopies and Société Biologie Cellulaire de France</licence></availability><date type="published" when="2011-02"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main">CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity</title><author xml:id="author-0000" role="corresp"><persName><forename type="first">Rose</forename><surname>Boutros</surname></persName><affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia<address><country key="AU"></country></address></affiliation><affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France<address><country key="FR"></country></address></affiliation><affiliation>Centre Pierre Potier, ITAV ‐ UMS3039 CRT‐CIV, 141 route d'Espagne, BP 50624, 31106 Toulouse Cedex 1, France and Children's Medical Research Institute, The University of Sydney, 214 Hawkesbury Road, Westmead, NSW 2145, Australia email bernard.ducommun@itav‐recherche.fr or rboutros@cmri.org.au)</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Corinne</forename><surname>Lorenzo</surname></persName><affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France<address><country key="FR"></country></address></affiliation><affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0002"><persName><forename type="first">Odile</forename><surname>Mondesert</surname></persName><affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France<address><country key="FR"></country></address></affiliation><affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0003"><persName><forename type="first">Alain</forename><surname>Jauneau</surname></persName><affiliation>CNRS, Plateforme Imagerie, Pôle de Biotechnologie Végétale, F‐31320 Castanet Tolosan, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0004"><persName><forename type="first">Vanessa</forename><surname>Oakes</surname></persName><affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia<address><country key="AU"></country></address></affiliation></author><author xml:id="author-0005"><persName><forename type="first">Christine</forename><surname>Dozier</surname></persName><affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France<address><country key="FR"></country></address></affiliation><affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France<address><country key="FR"></country></address></affiliation></author><author xml:id="author-0006"><persName><forename type="first">Brian</forename><surname>Gabrielli</surname></persName><affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia<address><country key="AU"></country></address></affiliation></author><author xml:id="author-0007" role="corresp"><persName><forename type="first">Bernard</forename><surname>Ducommun</surname></persName><affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France<address><country key="FR"></country></address></affiliation><affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France<address><country key="FR"></country></address></affiliation><affiliation>CHU Purpan, Service d'Hématologie, TSA 40031, F‐31059 Toulouse, France<address><country key="FR"></country></address></affiliation><affiliation>Centre Pierre Potier, ITAV ‐ UMS3039 CRT‐CIV, 141 route d'Espagne, BP 50624, 31106 Toulouse Cedex 1, France and Children's Medical Research Institute, The University of Sydney, 214 Hawkesbury Road, Westmead, NSW 2145, Australia email bernard.ducommun@itav‐recherche.fr or rboutros@cmri.org.au)</affiliation></author><idno type="istex">862895CE331F3CAEAE90459EA3274674D0D5D888</idno><idno type="ark">ark:/67375/WNG-QNVBG7DH-N</idno><idno type="DOI">10.1042/BC20100111</idno><idno type="unit">BOC80</idno><idno type="toTypesetVersion">file:BOC.BOC80.pdf</idno></analytic><monogr><title level="j" type="main">Biology of the Cell</title><title level="j" type="alt">BIOLOGY OF THE CELL</title><idno type="pISSN">0248-4900</idno><idno type="eISSN">1768-322X</idno><idno type="book-DOI">10.1111/(ISSN)1768-322X</idno><idno type="book-part-DOI">10.1111/boc.2011.103.issue-2</idno><idno type="product">BOC</idno><idno type="publisherDivision">ST</idno><imprint><biblScope unit="vol">103</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="55">55</biblScope><biblScope unit="page" to="68">68</biblScope><biblScope unit="page-count">14</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2011-02"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en" style="main"><p><hi rend="italic">Background information</hi>. CDC25 (cell division cycle 25) phosphatases function as activators of CDK (cyclin‐dependent kinase)–cyclin complexes to regulate progression through the CDC. We have recently identified a pool of CDC25B at the centrosome of interphase cells that plays a role in regulating centrosome numbers.</p><p><hi rend="italic">Results</hi>. In the present study, we demonstrate that CDC25B forms a close association with Ctn (centrin) proteins at the centrosome. This interaction involves both N‐ and C‐terminal regions of CDC25B and requires CDC25B binding to its CDK—cyclin substrates. However, the interaction is not dependent on the enzyme activity of CDC25B. Although CDC25B appears to bind indirectly to Ctn2, this association is pertinent to CDC25B localization at the centrosome. We further demonstrate that CDC25B plays a role in maintaining the overall integrity of the centrosome, by regulating the centrosome levels of multiple centrosome proteins, including that of Ctn2.</p><p><hi rend="italic">Conclusions</hi>. Our results therefore suggest that CDC25B associates with a Ctn2‐containing multiprotein complex in the cytoplasm, which targets it to the centrosome, where it plays a role in maintaining the centrosome levels of Ctn2 and a number of other centrosome components.</p></abstract><textClass><keywords xml:lang="en"><term xml:id="k1">CDC25B</term><term xml:id="k2">centrin (Ctn)</term><term xml:id="k3">centrosome</term><term xml:id="k4">cyclin‐dependent kinase (CDK)</term><term xml:id="k5">fluorescence lifetime imaging microscopy (FLIM)</term></keywords><keywords rend="tocHeading1"><term>Research Article</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/862895CE331F3CAEAE90459EA3274674D0D5D888/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1768-322X</doi><issn type="print">0248-4900</issn><issn type="electronic">1768-322X</issn><idGroup><id type="product" value="BOC"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="BIOLOGY OF THE CELL">Biology of the Cell</title></titleGroup></publicationMeta><publicationMeta level="part" position="02102"><doi origin="wiley">10.1111/boc.2011.103.issue-2</doi><numberingGroup><numbering type="journalVolume" number="103">103</numbering><numbering type="journalIssue" number="2">2</numbering></numberingGroup><coverDate startDate="2011-02">February 2011</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="1" status="forIssue"><doi origin="wiley">10.1042/BC20100111</doi><idGroup><id type="unit" value="BOC80"></id></idGroup><countGroup><count type="pageTotal" number="14"></count></countGroup><titleGroup><title type="tocHeading1">Research Article</title></titleGroup><copyright>2011 Société Française des Microscopies and Société Biologie Cellulaire de France</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:3.0.1 mode:FullText" date="2011-12-20"></event><event type="firstOnline" date="2012-01-03"></event><event type="publishedOnlineFinalForm" date="2012-01-03"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-08"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="55">55</numbering><numbering type="pageLast" number="68">68</numbering></numberingGroup><correspondenceTo>Centre Pierre Potier, ITAV ‐ UMS3039 CRT‐CIV, 141 route d'Espagne, BP 50624, 31106 Toulouse Cedex 1, France and Children's Medical Research Institute, The University of Sydney, 214 Hawkesbury Road, Westmead, NSW 2145, Australia email <email normalForm="bernard.ducommun@itav-recherche.fr">bernard.ducommun@itav‐recherche.fr</email> or <email>rboutros@cmri.org.au</email>)</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:BOC.BOC80.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 19 November 2010; Accepted 23 November 2010</unparsedEditorialHistory><countGroup><count type="figureTotal" number="6"></count><count type="tableTotal" number="0"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="46"></count><count type="linksCrossRef" number="125"></count></countGroup><titleGroup><title type="main">CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1 #a2" corresponding="yes"><personName><givenNames>Rose</givenNames><familyName>Boutros</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2 #a3"><personName><givenNames>Corinne</givenNames><familyName>Lorenzo</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a2 #a3"><personName><givenNames>Odile</givenNames><familyName>Mondesert</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a4"><personName><givenNames>Alain</givenNames><familyName>Jauneau</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1"><personName><givenNames>Vanessa</givenNames><familyName>Oakes</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a2 #a3"><personName><givenNames>Christine</givenNames><familyName>Dozier</familyName></personName></creator><creator creatorRole="author" xml:id="cr7" affiliationRef="#a1"><personName><givenNames>Brian</givenNames><familyName>Gabrielli</familyName></personName></creator><creator creatorRole="author" xml:id="cr8" affiliationRef="#a2 #a3 #a5" corresponding="yes"><personName><givenNames>Bernard</givenNames><familyName>Ducommun</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="AU"><unparsedAffiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="FR"><unparsedAffiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="FR"><unparsedAffiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="FR"><unparsedAffiliation>CNRS, Plateforme Imagerie, Pôle de Biotechnologie Végétale, F‐31320 Castanet Tolosan, France</unparsedAffiliation></affiliation><affiliation xml:id="a5" countryCode="FR"><unparsedAffiliation>CHU Purpan, Service d'Hématologie, TSA 40031, F‐31059 Toulouse, France</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">CDC25B</keyword><keyword xml:id="k2">centrin (Ctn)</keyword><keyword xml:id="k3">centrosome</keyword><keyword xml:id="k4">cyclin‐dependent kinase (CDK)</keyword><keyword xml:id="k5">fluorescence lifetime imaging microscopy (FLIM)</keyword></keywordGroup><supportingInformation><p>Supporting Information</p><supportingInfoItem><mediaResource alt="supporting info item" href="urn-x:wiley:02484900:media:boc80:boc_55_sm_suppl"></mediaResource><caption>Supporting info item</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main" xml:lang="en"><p><i>Background information</i>. CDC25 (cell division cycle 25) phosphatases function as activators of CDK (cyclin‐dependent kinase)–cyclin complexes to regulate progression through the CDC. We have recently identified a pool of CDC25B at the centrosome of interphase cells that plays a role in regulating centrosome numbers.</p><p><i>Results</i>. In the present study, we demonstrate that CDC25B forms a close association with Ctn (centrin) proteins at the centrosome. This interaction involves both N‐ and C‐terminal regions of CDC25B and requires CDC25B binding to its CDK—cyclin substrates. However, the interaction is not dependent on the enzyme activity of CDC25B. Although CDC25B appears to bind indirectly to Ctn2, this association is pertinent to CDC25B localization at the centrosome. We further demonstrate that CDC25B plays a role in maintaining the overall integrity of the centrosome, by regulating the centrosome levels of multiple centrosome proteins, including that of Ctn2.</p><p><i>Conclusions</i>. Our results therefore suggest that CDC25B associates with a Ctn2‐containing multiprotein complex in the cytoplasm, which targets it to the centrosome, where it plays a role in maintaining the centrosome levels of Ctn2 and a number of other centrosome components.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>CDC25B associates with a centrin 2‐containing complex and is involved in maintaining centrosome integrity</title></titleInfo><name type="personal"><namePart type="given">Rose</namePart><namePart type="family">Boutros</namePart><affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</affiliation><affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</affiliation><affiliation>E-mail: bernard.ducommun@itav‐recherche.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Corinne</namePart><namePart type="family">Lorenzo</namePart><affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</affiliation><affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Odile</namePart><namePart type="family">Mondesert</namePart><affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</affiliation><affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Alain</namePart><namePart type="family">Jauneau</namePart><affiliation>CNRS, Plateforme Imagerie, Pôle de Biotechnologie Végétale, F‐31320 Castanet Tolosan, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Vanessa</namePart><namePart type="family">Oakes</namePart><affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Christine</namePart><namePart type="family">Dozier</namePart><affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</affiliation><affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Brian</namePart><namePart type="family">Gabrielli</namePart><affiliation>Diamantina Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bernard</namePart><namePart type="family">Ducommun</namePart><affiliation>Université de Toulouse, LBCMCP, 118 route de Narbonne, F‐31062 Toulouse, France</affiliation><affiliation>Centre National de la Recherche Scientifique (CNRS), LBCMCP‐UMR5088, F‐31062 Toulouse, France</affiliation><affiliation>CHU Purpan, Service d'Hématologie, TSA 40031, F‐31059 Toulouse, France</affiliation><affiliation>E-mail: bernard.ducommun@itav‐recherche.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2011-02</dateIssued><edition>Received 19 November 2010; Accepted 23 November 2010</edition><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">6</extent><extent unit="tables">0</extent><extent unit="formulas">0</extent><extent unit="references">46</extent><extent unit="linksCrossRef">125</extent></physicalDescription><abstract lang="en">Background information. CDC25 (cell division cycle 25) phosphatases function as activators of CDK (cyclin‐dependent kinase)–cyclin complexes to regulate progression through the CDC. We have recently identified a pool of CDC25B at the centrosome of interphase cells that plays a role in regulating centrosome numbers. Results. In the present study, we demonstrate that CDC25B forms a close association with Ctn (centrin) proteins at the centrosome. This interaction involves both N‐ and C‐terminal regions of CDC25B and requires CDC25B binding to its CDK—cyclin substrates. However, the interaction is not dependent on the enzyme activity of CDC25B. Although CDC25B appears to bind indirectly to Ctn2, this association is pertinent to CDC25B localization at the centrosome. We further demonstrate that CDC25B plays a role in maintaining the overall integrity of the centrosome, by regulating the centrosome levels of multiple centrosome proteins, including that of Ctn2. Conclusions. Our results therefore suggest that CDC25B associates with a Ctn2‐containing multiprotein complex in the cytoplasm, which targets it to the centrosome, where it plays a role in maintaining the centrosome levels of Ctn2 and a number of other centrosome components.</abstract><subject lang="en"><genre>keywords</genre><topic>CDC25B</topic><topic>centrin (Ctn)</topic><topic>centrosome</topic><topic>cyclin‐dependent kinase (CDK)</topic><topic>fluorescence lifetime imaging microscopy (FLIM)</topic></subject><relatedItem type="host"><titleInfo><title>Biology of the Cell</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><note type="content"> Supporting InformationSupporting Info Item: Supporting info item - </note><identifier type="ISSN">0248-4900</identifier><identifier type="eISSN">1768-322X</identifier><identifier type="DOI">10.1111/(ISSN)1768-322X</identifier><identifier type="PublisherID">BOC</identifier><part><date>2011</date><detail type="volume"><caption>vol.</caption><number>103</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>55</start><end>68</end><total>14</total></extent></part></relatedItem><identifier type="istex">862895CE331F3CAEAE90459EA3274674D0D5D888</identifier><identifier type="ark">ark:/67375/WNG-QNVBG7DH-N</identifier><identifier type="DOI">10.1042/BC20100111</identifier><identifier type="ArticleID">BOC80</identifier><accessCondition type="use and reproduction" contentType="copyright">2011 Société Française des Microscopies and Société Biologie Cellulaire de France</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/862895CE331F3CAEAE90459EA3274674D0D5D888/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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