Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA
Identifieur interne : 000F68 ( Istex/Curation ); précédent : 000F67; suivant : 000F69Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA
Auteurs : L. A Newton [Australie] ; N. B Chilton [Australie] ; I. Beveridge [Australie] ; H. Hoste [France] ; P. Nansen [Danemark] ; R. B Gasser [Australie]Source :
- Acta Tropica [ 0001-706X ] ; 1998.
English descriptors
- KwdEn :
- Acta, Acta tropica, Adult adult adult, Australia victoria, Axei, Beveridge, Chabertia o6ina, Characteristic restriction patterns, Chilton, Circumcincta, Colubriformis, Contortus, Cooperia, Cooperia oncophora, Different sequence types, Drai, Endonuclease, Gasser, Genetic markers, Haemonchus, Haemonchus contortus, Hinfi, Hoste, Internal transcribed spacers, Lichtenfels, Molecular weight markers, Morphological criteria, Nematoda, Nematode, Nematodirus, Nlaiii, Oncophora, Order strongylida, Ostertagi, Ostertagia, Ostertagia ostertagi, Other strongylid nematodes, PCR-RFLP, Parasite, Parasitic nematodes of livestock, Parasitol, Polymerase, Polymerase chain reaction, Present study, Rdna, Restriction endonucleases, Restriction patterns, Ribosomal, Ribosomal DNA, Rsai, Sequence variation, Spacer, Spathiger, Species identification, Species level, Strongylid, Strongylid nematodes, Surnabada, Teladorsagia, Teladorsagia circumcincta, Trichostrongylidae, Trichostrongylus, Tropica.
- Teeft :
- Acta, Acta tropica, Adult adult adult, Australia victoria, Axei, Beveridge, Chabertia o6ina, Characteristic restriction patterns, Chilton, Circumcincta, Colubriformis, Contortus, Cooperia, Cooperia oncophora, Different sequence types, Drai, Endonuclease, Gasser, Genetic markers, Haemonchus, Haemonchus contortus, Hinfi, Hoste, Lichtenfels, Molecular weight markers, Morphological criteria, Nematoda, Nematode, Nematodirus, Nlaiii, Oncophora, Order strongylida, Ostertagi, Ostertagia, Ostertagia ostertagi, Other strongylid nematodes, Parasite, Parasitol, Polymerase, Polymerase chain reaction, Present study, Rdna, Restriction endonucleases, Restriction patterns, Ribosomal, Rsai, Sequence variation, Spacer, Spathiger, Species level, Strongylid, Strongylid nematodes, Surnabada, Teladorsagia, Teladorsagia circumcincta, Trichostrongylidae, Trichostrongylus, Tropica.
Abstract
Abstract: Twenty-four species of parasitic nematode (order Strongylida) from sheep, goats, cattle or pigs were characterised using a polymerase chain reaction-linked restriction fragment length polymorphism technique (PCR-RFLP). The ribosomal (r)DNA region spanning the first internal transcribed spacer (ITS-1), 5.8S rRNA gene and the second internal transcribed spacer (ITS-2) (designated ITS) was amplified from genomic DNA by polymerase chain reaction (PCR), digested separately with four restriction endonucleases (RsaI, HinfI, DraI or NlaIII) and the fragments separated by agarose gel electrophoresis. The PCR products amplified from all species appeared as a single band of ∼870 bp in size, except for Ostertagia ostertagi whose product was ∼1250 bp. The PCR-RFLP analysis of ITS revealed characteristic restriction patterns for all species, except for C. surnabada and C. oncophora which had identical patterns. The study demonstrated that ITS contains useful genetic markers for the identification of a range of strongylid nematodes of livestock. These markers should be of use in specific PCR assays for the identification of developmental stages of the parasites where morphological characters are unreliable.
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DOI: 10.1016/S0001-706X(97)00105-8
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Box 254, Fairfield, Victoria 3078, Australia.</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>1Present address: Macfarlane Burnet Centre for Medical Research, P.O. Box 254, Fairfield, Victoria 3078</wicri:regionArea></affiliation></author><author><name sortKey="Chilton, N B" sort="Chilton, N B" uniqKey="Chilton N" first="N. B" last="Chilton">N. B Chilton</name><affiliation wicri:level="1"><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030</wicri:regionArea></affiliation></author><author><name sortKey="Beveridge, I" sort="Beveridge, I" uniqKey="Beveridge I" first="I" last="Beveridge">I. 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A Newton</name><affiliation wicri:level="1"><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>1Present address: Macfarlane Burnet Centre for Medical Research, P.O. Box 254, Fairfield, Victoria 3078, Australia.</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>1Present address: Macfarlane Burnet Centre for Medical Research, P.O. Box 254, Fairfield, Victoria 3078</wicri:regionArea></affiliation></author><author><name sortKey="Chilton, N B" sort="Chilton, N B" uniqKey="Chilton N" first="N. B" last="Chilton">N. B Chilton</name><affiliation wicri:level="1"><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030</wicri:regionArea></affiliation></author><author><name sortKey="Beveridge, I" sort="Beveridge, I" uniqKey="Beveridge I" first="I" last="Beveridge">I. Beveridge</name><affiliation wicri:level="1"><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030</wicri:regionArea></affiliation></author><author><name sortKey="Hoste, H" sort="Hoste, H" uniqKey="Hoste H" first="H" last="Hoste">H. 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Nansen</name><affiliation wicri:level="1"><mods:affiliation>Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark</mods:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C</wicri:regionArea></affiliation></author><author><name sortKey="Gasser, R B" sort="Gasser, R B" uniqKey="Gasser R" first="R. B" last="Gasser">R. B Gasser</name><affiliation wicri:level="1"><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Corresponding author. E-mail: gasser@ariel.ucs.unimelb.edu.au</mods:affiliation><country wicri:rule="url">Australie</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Acta Tropica</title><title level="j" type="abbrev">ACTROP</title><idno type="ISSN">0001-706X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">69</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="15">15</biblScope></imprint><idno type="ISSN">0001-706X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0001-706X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acta</term><term>Acta tropica</term><term>Adult adult adult</term><term>Australia victoria</term><term>Axei</term><term>Beveridge</term><term>Chabertia o6ina</term><term>Characteristic restriction patterns</term><term>Chilton</term><term>Circumcincta</term><term>Colubriformis</term><term>Contortus</term><term>Cooperia</term><term>Cooperia oncophora</term><term>Different sequence types</term><term>Drai</term><term>Endonuclease</term><term>Gasser</term><term>Genetic markers</term><term>Haemonchus</term><term>Haemonchus contortus</term><term>Hinfi</term><term>Hoste</term><term>Internal transcribed spacers</term><term>Lichtenfels</term><term>Molecular weight markers</term><term>Morphological criteria</term><term>Nematoda</term><term>Nematode</term><term>Nematodirus</term><term>Nlaiii</term><term>Oncophora</term><term>Order strongylida</term><term>Ostertagi</term><term>Ostertagia</term><term>Ostertagia ostertagi</term><term>Other strongylid nematodes</term><term>PCR-RFLP</term><term>Parasite</term><term>Parasitic nematodes of livestock</term><term>Parasitol</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Present study</term><term>Rdna</term><term>Restriction endonucleases</term><term>Restriction patterns</term><term>Ribosomal</term><term>Ribosomal DNA</term><term>Rsai</term><term>Sequence variation</term><term>Spacer</term><term>Spathiger</term><term>Species identification</term><term>Species level</term><term>Strongylid</term><term>Strongylid nematodes</term><term>Surnabada</term><term>Teladorsagia</term><term>Teladorsagia circumcincta</term><term>Trichostrongylidae</term><term>Trichostrongylus</term><term>Tropica</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acta</term><term>Acta tropica</term><term>Adult adult adult</term><term>Australia victoria</term><term>Axei</term><term>Beveridge</term><term>Chabertia o6ina</term><term>Characteristic restriction patterns</term><term>Chilton</term><term>Circumcincta</term><term>Colubriformis</term><term>Contortus</term><term>Cooperia</term><term>Cooperia oncophora</term><term>Different sequence types</term><term>Drai</term><term>Endonuclease</term><term>Gasser</term><term>Genetic markers</term><term>Haemonchus</term><term>Haemonchus contortus</term><term>Hinfi</term><term>Hoste</term><term>Lichtenfels</term><term>Molecular weight markers</term><term>Morphological criteria</term><term>Nematoda</term><term>Nematode</term><term>Nematodirus</term><term>Nlaiii</term><term>Oncophora</term><term>Order strongylida</term><term>Ostertagi</term><term>Ostertagia</term><term>Ostertagia ostertagi</term><term>Other strongylid nematodes</term><term>Parasite</term><term>Parasitol</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Present study</term><term>Rdna</term><term>Restriction endonucleases</term><term>Restriction patterns</term><term>Ribosomal</term><term>Rsai</term><term>Sequence variation</term><term>Spacer</term><term>Spathiger</term><term>Species level</term><term>Strongylid</term><term>Strongylid nematodes</term><term>Surnabada</term><term>Teladorsagia</term><term>Teladorsagia circumcincta</term><term>Trichostrongylidae</term><term>Trichostrongylus</term><term>Tropica</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Twenty-four species of parasitic nematode (order Strongylida) from sheep, goats, cattle or pigs were characterised using a polymerase chain reaction-linked restriction fragment length polymorphism technique (PCR-RFLP). The ribosomal (r)DNA region spanning the first internal transcribed spacer (ITS-1), 5.8S rRNA gene and the second internal transcribed spacer (ITS-2) (designated ITS) was amplified from genomic DNA by polymerase chain reaction (PCR), digested separately with four restriction endonucleases (RsaI, HinfI, DraI or NlaIII) and the fragments separated by agarose gel electrophoresis. The PCR products amplified from all species appeared as a single band of ∼870 bp in size, except for Ostertagia ostertagi whose product was ∼1250 bp. The PCR-RFLP analysis of ITS revealed characteristic restriction patterns for all species, except for C. surnabada and C. oncophora which had identical patterns. The study demonstrated that ITS contains useful genetic markers for the identification of a range of strongylid nematodes of livestock. These markers should be of use in specific PCR assays for the identification of developmental stages of the parasites where morphological characters are unreliable.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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