Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA
Identifieur interne : 000F68 ( Istex/Corpus ); précédent : 000F67; suivant : 000F69Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA
Auteurs : L. A Newton ; N. B Chilton ; I. Beveridge ; H. Hoste ; P. Nansen ; R. B GasserSource :
- Acta Tropica [ 0001-706X ] ; 1998.
English descriptors
- KwdEn :
- Acta, Acta tropica, Adult adult adult, Australia victoria, Axei, Beveridge, Chabertia o6ina, Characteristic restriction patterns, Chilton, Circumcincta, Colubriformis, Contortus, Cooperia, Cooperia oncophora, Different sequence types, Drai, Endonuclease, Gasser, Genetic markers, Haemonchus, Haemonchus contortus, Hinfi, Hoste, Internal transcribed spacers, Lichtenfels, Molecular weight markers, Morphological criteria, Nematoda, Nematode, Nematodirus, Nlaiii, Oncophora, Order strongylida, Ostertagi, Ostertagia, Ostertagia ostertagi, Other strongylid nematodes, PCR-RFLP, Parasite, Parasitic nematodes of livestock, Parasitol, Polymerase, Polymerase chain reaction, Present study, Rdna, Restriction endonucleases, Restriction patterns, Ribosomal, Ribosomal DNA, Rsai, Sequence variation, Spacer, Spathiger, Species identification, Species level, Strongylid, Strongylid nematodes, Surnabada, Teladorsagia, Teladorsagia circumcincta, Trichostrongylidae, Trichostrongylus, Tropica.
- Teeft :
- Acta, Acta tropica, Adult adult adult, Australia victoria, Axei, Beveridge, Chabertia o6ina, Characteristic restriction patterns, Chilton, Circumcincta, Colubriformis, Contortus, Cooperia, Cooperia oncophora, Different sequence types, Drai, Endonuclease, Gasser, Genetic markers, Haemonchus, Haemonchus contortus, Hinfi, Hoste, Lichtenfels, Molecular weight markers, Morphological criteria, Nematoda, Nematode, Nematodirus, Nlaiii, Oncophora, Order strongylida, Ostertagi, Ostertagia, Ostertagia ostertagi, Other strongylid nematodes, Parasite, Parasitol, Polymerase, Polymerase chain reaction, Present study, Rdna, Restriction endonucleases, Restriction patterns, Ribosomal, Rsai, Sequence variation, Spacer, Spathiger, Species level, Strongylid, Strongylid nematodes, Surnabada, Teladorsagia, Teladorsagia circumcincta, Trichostrongylidae, Trichostrongylus, Tropica.
Abstract
Abstract: Twenty-four species of parasitic nematode (order Strongylida) from sheep, goats, cattle or pigs were characterised using a polymerase chain reaction-linked restriction fragment length polymorphism technique (PCR-RFLP). The ribosomal (r)DNA region spanning the first internal transcribed spacer (ITS-1), 5.8S rRNA gene and the second internal transcribed spacer (ITS-2) (designated ITS) was amplified from genomic DNA by polymerase chain reaction (PCR), digested separately with four restriction endonucleases (RsaI, HinfI, DraI or NlaIII) and the fragments separated by agarose gel electrophoresis. The PCR products amplified from all species appeared as a single band of ∼870 bp in size, except for Ostertagia ostertagi whose product was ∼1250 bp. The PCR-RFLP analysis of ITS revealed characteristic restriction patterns for all species, except for C. surnabada and C. oncophora which had identical patterns. The study demonstrated that ITS contains useful genetic markers for the identification of a range of strongylid nematodes of livestock. These markers should be of use in specific PCR assays for the identification of developmental stages of the parasites where morphological characters are unreliable.
Url:
DOI: 10.1016/S0001-706X(97)00105-8
Links to Exploration step
ISTEX:5321BECAD8BC24065B1F953F89E0E61A65178255Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA</title><author><name sortKey="Newton, L A" sort="Newton, L A" uniqKey="Newton L" first="L. A" last="Newton">L. A Newton</name><affiliation><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation></affiliation><affiliation><mods:affiliation>1Present address: Macfarlane Burnet Centre for Medical Research, P.O. Box 254, Fairfield, Victoria 3078, Australia.</mods:affiliation></affiliation></author><author><name sortKey="Chilton, N B" sort="Chilton, N B" uniqKey="Chilton N" first="N. B" last="Chilton">N. B Chilton</name><affiliation><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation></affiliation></author><author><name sortKey="Beveridge, I" sort="Beveridge, I" uniqKey="Beveridge I" first="I" last="Beveridge">I. Beveridge</name><affiliation><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation></affiliation></author><author><name sortKey="Hoste, H" sort="Hoste, H" uniqKey="Hoste H" first="H" last="Hoste">H. Hoste</name><affiliation><mods:affiliation>Institut National de la Recherche Agronomique, Station de Pathologie Aviaire et de Parasitologie, Centre de Tours, 37380 Nouzilly, France</mods:affiliation></affiliation></author><author><name sortKey="Nansen, P" sort="Nansen, P" uniqKey="Nansen P" first="P" last="Nansen">P. Nansen</name><affiliation><mods:affiliation>Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark</mods:affiliation></affiliation></author><author><name sortKey="Gasser, R B" sort="Gasser, R B" uniqKey="Gasser R" first="R. B" last="Gasser">R. B Gasser</name><affiliation><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation></affiliation><affiliation><mods:affiliation>Corresponding author. 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A Newton</name><affiliation><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation></affiliation><affiliation><mods:affiliation>1Present address: Macfarlane Burnet Centre for Medical Research, P.O. Box 254, Fairfield, Victoria 3078, Australia.</mods:affiliation></affiliation></author><author><name sortKey="Chilton, N B" sort="Chilton, N B" uniqKey="Chilton N" first="N. B" last="Chilton">N. B Chilton</name><affiliation><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation></affiliation></author><author><name sortKey="Beveridge, I" sort="Beveridge, I" uniqKey="Beveridge I" first="I" last="Beveridge">I. Beveridge</name><affiliation><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation></affiliation></author><author><name sortKey="Hoste, H" sort="Hoste, H" uniqKey="Hoste H" first="H" last="Hoste">H. Hoste</name><affiliation><mods:affiliation>Institut National de la Recherche Agronomique, Station de Pathologie Aviaire et de Parasitologie, Centre de Tours, 37380 Nouzilly, France</mods:affiliation></affiliation></author><author><name sortKey="Nansen, P" sort="Nansen, P" uniqKey="Nansen P" first="P" last="Nansen">P. Nansen</name><affiliation><mods:affiliation>Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark</mods:affiliation></affiliation></author><author><name sortKey="Gasser, R B" sort="Gasser, R B" uniqKey="Gasser R" first="R. B" last="Gasser">R. B Gasser</name><affiliation><mods:affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</mods:affiliation></affiliation><affiliation><mods:affiliation>Corresponding author. E-mail: gasser@ariel.ucs.unimelb.edu.au</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Acta Tropica</title><title level="j" type="abbrev">ACTROP</title><idno type="ISSN">0001-706X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">69</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="15">15</biblScope></imprint><idno type="ISSN">0001-706X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0001-706X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acta</term><term>Acta tropica</term><term>Adult adult adult</term><term>Australia victoria</term><term>Axei</term><term>Beveridge</term><term>Chabertia o6ina</term><term>Characteristic restriction patterns</term><term>Chilton</term><term>Circumcincta</term><term>Colubriformis</term><term>Contortus</term><term>Cooperia</term><term>Cooperia oncophora</term><term>Different sequence types</term><term>Drai</term><term>Endonuclease</term><term>Gasser</term><term>Genetic markers</term><term>Haemonchus</term><term>Haemonchus contortus</term><term>Hinfi</term><term>Hoste</term><term>Internal transcribed spacers</term><term>Lichtenfels</term><term>Molecular weight markers</term><term>Morphological criteria</term><term>Nematoda</term><term>Nematode</term><term>Nematodirus</term><term>Nlaiii</term><term>Oncophora</term><term>Order strongylida</term><term>Ostertagi</term><term>Ostertagia</term><term>Ostertagia ostertagi</term><term>Other strongylid nematodes</term><term>PCR-RFLP</term><term>Parasite</term><term>Parasitic nematodes of livestock</term><term>Parasitol</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Present study</term><term>Rdna</term><term>Restriction endonucleases</term><term>Restriction patterns</term><term>Ribosomal</term><term>Ribosomal DNA</term><term>Rsai</term><term>Sequence variation</term><term>Spacer</term><term>Spathiger</term><term>Species identification</term><term>Species level</term><term>Strongylid</term><term>Strongylid nematodes</term><term>Surnabada</term><term>Teladorsagia</term><term>Teladorsagia circumcincta</term><term>Trichostrongylidae</term><term>Trichostrongylus</term><term>Tropica</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acta</term><term>Acta tropica</term><term>Adult adult adult</term><term>Australia victoria</term><term>Axei</term><term>Beveridge</term><term>Chabertia o6ina</term><term>Characteristic restriction patterns</term><term>Chilton</term><term>Circumcincta</term><term>Colubriformis</term><term>Contortus</term><term>Cooperia</term><term>Cooperia oncophora</term><term>Different sequence types</term><term>Drai</term><term>Endonuclease</term><term>Gasser</term><term>Genetic markers</term><term>Haemonchus</term><term>Haemonchus contortus</term><term>Hinfi</term><term>Hoste</term><term>Lichtenfels</term><term>Molecular weight markers</term><term>Morphological criteria</term><term>Nematoda</term><term>Nematode</term><term>Nematodirus</term><term>Nlaiii</term><term>Oncophora</term><term>Order strongylida</term><term>Ostertagi</term><term>Ostertagia</term><term>Ostertagia ostertagi</term><term>Other strongylid nematodes</term><term>Parasite</term><term>Parasitol</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Present study</term><term>Rdna</term><term>Restriction endonucleases</term><term>Restriction patterns</term><term>Ribosomal</term><term>Rsai</term><term>Sequence variation</term><term>Spacer</term><term>Spathiger</term><term>Species level</term><term>Strongylid</term><term>Strongylid nematodes</term><term>Surnabada</term><term>Teladorsagia</term><term>Teladorsagia circumcincta</term><term>Trichostrongylidae</term><term>Trichostrongylus</term><term>Tropica</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Twenty-four species of parasitic nematode (order Strongylida) from sheep, goats, cattle or pigs were characterised using a polymerase chain reaction-linked restriction fragment length polymorphism technique (PCR-RFLP). The ribosomal (r)DNA region spanning the first internal transcribed spacer (ITS-1), 5.8S rRNA gene and the second internal transcribed spacer (ITS-2) (designated ITS) was amplified from genomic DNA by polymerase chain reaction (PCR), digested separately with four restriction endonucleases (RsaI, HinfI, DraI or NlaIII) and the fragments separated by agarose gel electrophoresis. The PCR products amplified from all species appeared as a single band of ∼870 bp in size, except for Ostertagia ostertagi whose product was ∼1250 bp. The PCR-RFLP analysis of ITS revealed characteristic restriction patterns for all species, except for C. surnabada and C. oncophora which had identical patterns. The study demonstrated that ITS contains useful genetic markers for the identification of a range of strongylid nematodes of livestock. These markers should be of use in specific PCR assays for the identification of developmental stages of the parasites where morphological characters are unreliable.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>nematode</json:string><json:string>parasitol</json:string><json:string>rdna</json:string><json:string>endonuclease</json:string><json:string>strongylid</json:string><json:string>ribosomal</json:string><json:string>acta</json:string><json:string>chilton</json:string><json:string>lichtenfels</json:string><json:string>ostertagi</json:string><json:string>tropica</json:string><json:string>acta tropica</json:string><json:string>strongylid nematodes</json:string><json:string>restriction patterns</json:string><json:string>contortus</json:string><json:string>cooperia</json:string><json:string>gasser</json:string><json:string>rsai</json:string><json:string>nematoda</json:string><json:string>haemonchus</json:string><json:string>nlaiii</json:string><json:string>trichostrongylidae</json:string><json:string>spathiger</json:string><json:string>oncophora</json:string><json:string>beveridge</json:string><json:string>nematodirus</json:string><json:string>hinfi</json:string><json:string>surnabada</json:string><json:string>ostertagia</json:string><json:string>trichostrongylus</json:string><json:string>axei</json:string><json:string>polymerase</json:string><json:string>drai</json:string><json:string>genetic markers</json:string><json:string>circumcincta</json:string><json:string>colubriformis</json:string><json:string>teladorsagia</json:string><json:string>parasite</json:string><json:string>australia victoria</json:string><json:string>cooperia oncophora</json:string><json:string>species level</json:string><json:string>hoste</json:string><json:string>different sequence types</json:string><json:string>characteristic restriction patterns</json:string><json:string>haemonchus contortus</json:string><json:string>restriction endonucleases</json:string><json:string>ostertagia ostertagi</json:string><json:string>molecular weight markers</json:string><json:string>adult adult adult</json:string><json:string>present study</json:string><json:string>teladorsagia circumcincta</json:string><json:string>chabertia o6ina</json:string><json:string>polymerase chain reaction</json:string><json:string>sequence variation</json:string><json:string>other strongylid nematodes</json:string><json:string>morphological criteria</json:string><json:string>order strongylida</json:string><json:string>spacer</json:string></teeft></keywords><author><json:item><name>L.A Newton</name><affiliations><json:string>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</json:string><json:string>1Present address: Macfarlane Burnet Centre for Medical Research, P.O. Box 254, Fairfield, Victoria 3078, Australia.</json:string></affiliations></json:item><json:item><name>N.B Chilton</name><affiliations><json:string>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</json:string></affiliations></json:item><json:item><name>I Beveridge</name><affiliations><json:string>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</json:string></affiliations></json:item><json:item><name>H Hoste</name><affiliations><json:string>Institut National de la Recherche Agronomique, Station de Pathologie Aviaire et de Parasitologie, Centre de Tours, 37380 Nouzilly, France</json:string></affiliations></json:item><json:item><name>P Nansen</name><affiliations><json:string>Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark</json:string></affiliations></json:item><json:item><name>R.B Gasser</name><affiliations><json:string>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</json:string><json:string>Corresponding author. E-mail: gasser@ariel.ucs.unimelb.edu.au</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Ribosomal DNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Internal transcribed spacers</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PCR-RFLP</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Genetic markers</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Species identification</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Parasitic nematodes of livestock</value></json:item></subject><articleId><json:string>752</json:string></articleId><arkIstex>ark:/67375/6H6-C1RCDBM5-J</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Twenty-four species of parasitic nematode (order Strongylida) from sheep, goats, cattle or pigs were characterised using a polymerase chain reaction-linked restriction fragment length polymorphism technique (PCR-RFLP). The ribosomal (r)DNA region spanning the first internal transcribed spacer (ITS-1), 5.8S rRNA gene and the second internal transcribed spacer (ITS-2) (designated ITS) was amplified from genomic DNA by polymerase chain reaction (PCR), digested separately with four restriction endonucleases (RsaI, HinfI, DraI or NlaIII) and the fragments separated by agarose gel electrophoresis. The PCR products amplified from all species appeared as a single band of ∼870 bp in size, except for Ostertagia ostertagi whose product was ∼1250 bp. The PCR-RFLP analysis of ITS revealed characteristic restriction patterns for all species, except for C. surnabada and C. oncophora which had identical patterns. The study demonstrated that ITS contains useful genetic markers for the identification of a range of strongylid nematodes of livestock. These markers should be of use in specific PCR assays for the identification of developmental stages of the parasites where morphological characters are unreliable.</abstract><qualityIndicators><score>8.976</score><pdfWordCount>4912</pdfWordCount><pdfCharCount>32289</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>15</pdfPageCount><pdfPageSize>587 x 754 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>172</abstractWordCount><abstractCharCount>1221</abstractCharCount><keywordCount>6</keywordCount></qualityIndicators><title>Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA</title><pmid><json:string>9588237</json:string></pmid><pii><json:string>S0001-706X(97)00105-8</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Acta Tropica</title><language><json:string>unknown</json:string></language><publicationDate>1998</publicationDate><issn><json:string>0001-706X</json:string></issn><pii><json:string>S0001-706X(00)X0033-2</json:string></pii><volume>69</volume><issue>1</issue><pages><first>1</first><last>15</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>18S</json:string><json:string>1998</json:string><json:string>28S</json:string><json:string>1250</json:string></date><geogName></geogName><orgName><json:string>Australia Victoria, Australia Victoria, Australia Copenhagen, Denmark Cattle Cattle Cattle Cattle Sheep Sheep Adult Adult Adult Adult Adult</json:string><json:string>Australia New South Wales, Australia Nouzilly, France Arkansas, USA Nouzilly, France Sheep Sheep Goat Adult Adult Adult Cho</json:string><json:string>Australia Copenhagen, Denmark Zurich, Switzerland</json:string><json:string>Department of Employment, Education and Training</json:string><json:string>Denmark Oec</json:string><json:string>Australia Oe</json:string><json:string>Australia Oed</json:string><json:string>Department of Industry, Science and Tourism</json:string><json:string>Collaborative Research Program</json:string><json:string>Germany Victoria, Australia Victoria, Australia Cattle</json:string><json:string>Society for Parasitology</json:string><json:string>Australia Oev</json:string><json:string>Australia Scotland New Zealand Victoria, Australia New Zealand Germany O</json:string><json:string>Australia Victoria, Australia Victoria, Australia Arkansas, USA Nouzilly, France The Netherlands Sheep Sheep Adult Adult Nf</json:string><json:string>International Senior Research Fellowship</json:string><json:string>University of Melbourne</json:string><json:string>Denmark Oeq</json:string><json:string>Veterinary Research Fund, Department of Veterinary Science</json:string><json:string>Denmark Oed</json:string><json:string>Australia Germany Trichostrongylidae Haemonchinae Haemonchus</json:string><json:string>Australia New South Wales, Australia China France Germany Malaysia Switzerland South Africa UK Arkansas, USA Zimbabwe New Zealand New South Wales, Australia Queensland, Australia The Netherlands Sheep Sheep Sheep Sheep Sheep Sheep Adult</json:string><json:string>Australian Research Council</json:string></orgName><orgName_funder><json:string>Australian Research Council</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Lisa Newton</json:string><json:string>N. Anderson</json:string><json:string>T. Yazwinski</json:string><json:string>S.A. Henriksen</json:string><json:string>P.O. Box</json:string><json:string>H. Hoste</json:string><json:string>M. Varady</json:string><json:string>B. Copeman</json:string><json:string>D. Barth</json:string><json:string>M. Roos</json:string><json:string>H. Hertzberg</json:string><json:string>Highway</json:string><json:string>Elmer Cetus</json:string><json:string>C. Morrow</json:string><json:string>RsaI</json:string><json:string>J. Wicker</json:string><json:string>E. Meeusen</json:string><json:string>R. Coop</json:string><json:string>Danish Centre</json:string><json:string>Gel</json:string><json:string>NlaIII</json:string><json:string>B. Besier</json:string><json:string>J. Eckert</json:string><json:string>G. Allerton</json:string><json:string>W. Pomroy</json:string><json:string>R. Gasser</json:string><json:string>Jan VanWyk</json:string><json:string>L. LeJambre</json:string></persName><placeName><json:string>Switzerland</json:string><json:string>Germany</json:string><json:string>Australia</json:string><json:string>China</json:string><json:string>Wales</json:string><json:string>South Africa</json:string><json:string>Puri</json:string><json:string>Denmark</json:string><json:string>New Zealand</json:string><json:string>France</json:string><json:string>Copenhagen</json:string><json:string>Netherlands</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Mullis et al., 1986</json:string><json:string>Isenstein, 1971</json:string><json:string>Lichtenfels et al., 1988</json:string><json:string>Wesson et al., 1992</json:string><json:string>Callaghan and Beh, 1994a,b</json:string><json:string>Goggin, 1994</json:string><json:string>Schlotterer and Tautz, 1994</json:string><json:string>Gasser et al., 1996b</json:string><json:string>Sommer, 1996</json:string><json:string>Bowles and McManus, 1993</json:string><json:string>Christensen et al., 1994</json:string><json:string>Chilton et al., 1995</json:string><json:string>Barth, 1991</json:string><json:string>Lichtenfels, 1977</json:string><json:string>Morales and Cabaret, 1985</json:string><json:string>Hoste et al., 1995</json:string><json:string>Bowles et al., 1995</json:string><json:string>Lichtenfels and Hoberg, 1993</json:string><json:string>Gasser et al., 1996a</json:string><json:string>Gibbons, 1981</json:string><json:string>Gasser et al., 1994</json:string><json:string>Durette-Desset et al., 1994</json:string><json:string>15–30%; Hillis and Dixon, 1991</json:string><json:string>Gasser and Chilton, 1995</json:string><json:string>Hillis and Dixon, 1991</json:string><json:string>Zarlenga et al., 1994a,b</json:string><json:string>Stringfellow, 1970</json:string><json:string>Gasser et al., 1993</json:string><json:string>Schnieder et al., 1996</json:string><json:string>Lichtenfels, 1986</json:string><json:string>Whitlock and Le Jambre, 1981</json:string><json:string>Stevenson et al., 1996</json:string><json:string>Luton et al., 1992</json:string><json:string>Kane and Rollinson, 1994</json:string><json:string>Campbell et al., 1995</json:string><json:string>Lancaster et al., 1983</json:string><json:string>Lichtenfels, 1980</json:string><json:string>L.A. Newton et al.</json:string><json:string>see Gerbi, 1986</json:string><json:string>Morgan and Blair, 1995</json:string><json:string>Chilton et al., 1997</json:string><json:string>Stevenson et al., 1995</json:string><json:string>Adlard et al., 1993</json:string><json:string>Gasser and Hoste, 1995</json:string><json:string>Southern, 1975</json:string><json:string>Skrjabin et al., 1952</json:string><json:string>Hoberg and Lichtenfels, 1994</json:string><json:string>Hung et al., 1996</json:string><json:string>Andrews and Beveridge, 1990</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-C1RCDBM5-J</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - tropical medicine</json:string><json:string>2 - parasitology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - tropical medicine</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Infectious Diseases</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - General Medicine</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Parasitology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - invertebres</json:string></inist></categories><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><doi><json:string>10.1016/S0001-706X(97)00105-8</json:string></doi><id>5321BECAD8BC24065B1F953F89E0E61A65178255</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/5321BECAD8BC24065B1F953F89E0E61A65178255/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/5321BECAD8BC24065B1F953F89E0E61A65178255/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/5321BECAD8BC24065B1F953F89E0E61A65178255/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1998 Elsevier Science B.V.</p></availability><date>1998</date></publicationStmt><notesStmt><note type="content">Fig. 1: Gel showing ITS PCR products of Ostertagia erschowi (lane 1), O. leptospicularis (lane 2) and the ∼1250 bp product of O. ostertagi (lane 3). The ITS PCR products of all species examined in this study, except O. ostertagi, were ∼870 bp in size.</note><note type="content">Fig. 2: Example of an agarose gel showing a lack of variation in the ITS restriction patterns (using endonucleases HinfI and RsaI) among different samples of Haemonchus contortus (Hc9, Hc10, Hc13, Hc15, Hc16, Hc17, Hc21, Hc22, Hc24, Hc26, Hc31 and Hc33, lanes 1–12, respectively). ΦX174-HaeIII molecular weight markers (Promega) represented with m.</note><note type="content">Fig. 3: Examples of the PCR-RFLP analysis of ITS rDNA with the endonucleases RsaI and NlaIII showing interspecific differences in restriction patterns. Lanes: Teladorsagia circumcincta (1); Ostertagia ostertagi (2); O. leptospicularis (3); O. erschowi (4); Hyostrongylus rubidus (5); Oesophagostomum radiatum (6); Oe. venulosum (7); Oe. columbianum (8); Oe. dentatum (9); Oe. quadrispinulatum (10); Nematodirus spathiger (11); N. filicollis (12); N. helvetianus (13); and N. battus (14). Ordinate values indicate approximate molecular weight in base pairs. A mix of ΦX174-HaeIII and pGEM™ molecular weight markers (Promega) represented with M.</note><note type="content">Fig. 4: Schematic representation of the ITS rDNA restriction patterns produced for 24 species of strongylid nematodes of livestock (sheep/goat, cattle or pig). Individual boxes contain patterns produced for restriction endonucleases DraI, NlaIII, HinfI and RsaI. Molecular weights in bp (left). Size of undigested PCR products was ∼870 bp for all species except O. ostertagi (∼1250 bp). Cooperia oncophora and C. surnabada. (represented by C) could not be differentiated by their restriction pattern using any of the four endonucleases. Restriction patterns that were representative for multiple species are designated with `G': G1: Haemonchus contortus, Nematodirus battus, N. filicollis, N. spathiger, Oesophagostomum columbianum and Chabertia ovina. G2: Trichostrongylus axei, T. colubriformis and T. vitrinus. G3: T. axei and T. colubriformis. G4: T. axei and T. vitrinus. G5: N. spathiger and N. helvetianus. G6: Oesophagostomum dentatum and Oe. quadrispinulatum. G7: Haemonchus placei, N. helvetianus and N. spathiger. G8: Cooperia oncophora, C. surnabada and C. punctata. ΦX174-HaeIII molecular weight markers (Promega) represented with m.</note><note type="content">Table 1: Parasite material</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA</title><author xml:id="author-0000"><persName><forename type="first">L.A</forename><surname>Newton</surname></persName><affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</affiliation><affiliation>1Present address: Macfarlane Burnet Centre for Medical Research, P.O. Box 254, Fairfield, Victoria 3078, Australia.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">N.B</forename><surname>Chilton</surname></persName><affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</affiliation></author><author xml:id="author-0002"><persName><forename type="first">I</forename><surname>Beveridge</surname></persName><affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</affiliation></author><author xml:id="author-0003"><persName><forename type="first">H</forename><surname>Hoste</surname></persName><affiliation>Institut National de la Recherche Agronomique, Station de Pathologie Aviaire et de Parasitologie, Centre de Tours, 37380 Nouzilly, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">P</forename><surname>Nansen</surname></persName><affiliation>Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark</affiliation></author><author xml:id="author-0005"><persName><forename type="first">R.B</forename><surname>Gasser</surname></persName><email>gasser@ariel.ucs.unimelb.edu.au</email><affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</affiliation></author><idno type="istex">5321BECAD8BC24065B1F953F89E0E61A65178255</idno><idno type="DOI">10.1016/S0001-706X(97)00105-8</idno><idno type="PII">S0001-706X(97)00105-8</idno><idno type="ArticleID">752</idno></analytic><monogr><title level="j">Acta Tropica</title><title level="j" type="abbrev">ACTROP</title><idno type="pISSN">0001-706X</idno><idno type="PII">S0001-706X(00)X0033-2</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998"></date><biblScope unit="volume">69</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="15">15</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1998</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Twenty-four species of parasitic nematode (order Strongylida) from sheep, goats, cattle or pigs were characterised using a polymerase chain reaction-linked restriction fragment length polymorphism technique (PCR-RFLP). The ribosomal (r)DNA region spanning the first internal transcribed spacer (ITS-1), 5.8S rRNA gene and the second internal transcribed spacer (ITS-2) (designated ITS) was amplified from genomic DNA by polymerase chain reaction (PCR), digested separately with four restriction endonucleases (RsaI, HinfI, DraI or NlaIII) and the fragments separated by agarose gel electrophoresis. The PCR products amplified from all species appeared as a single band of ∼870 bp in size, except for Ostertagia ostertagi whose product was ∼1250 bp. The PCR-RFLP analysis of ITS revealed characteristic restriction patterns for all species, except for C. surnabada and C. oncophora which had identical patterns. The study demonstrated that ITS contains useful genetic markers for the identification of a range of strongylid nematodes of livestock. These markers should be of use in specific PCR assays for the identification of developmental stages of the parasites where morphological characters are unreliable.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Ribosomal DNA</term></item><item><term>Internal transcribed spacers</term></item><item><term>PCR-RFLP</term></item><item><term>Genetic markers</term></item><item><term>Species identification</term></item><item><term>Parasitic nematodes of livestock</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1997-07-23">Modified</change><change when="1998">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/5321BECAD8BC24065B1F953F89E0E61A65178255/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>ACTROP</jid><aid>752</aid><ce:pii>S0001-706X(97)00105-8</ce:pii><ce:doi>10.1016/S0001-706X(97)00105-8</ce:doi><ce:copyright year="1998" type="full-transfer">Elsevier Science B.V.</ce:copyright></item-info><head><ce:title>Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA</ce:title><ce:author-group><ce:author><ce:given-name>L.A</ce:given-name><ce:surname>Newton</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>N.B</ce:given-name><ce:surname>Chilton</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref></ce:author><ce:author><ce:given-name>I</ce:given-name><ce:surname>Beveridge</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref></ce:author><ce:author><ce:given-name>H</ce:given-name><ce:surname>Hoste</ce:surname><ce:cross-ref refid="AFF2">b</ce:cross-ref></ce:author><ce:author><ce:given-name>P</ce:given-name><ce:surname>Nansen</ce:surname><ce:cross-ref refid="AFF3">c</ce:cross-ref></ce:author><ce:author><ce:given-name>R.B</ce:given-name><ce:surname>Gasser</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Institut National de la Recherche Agronomique, Station de Pathologie Aviaire et de Parasitologie, Centre de Tours, 37380 Nouzilly, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. E-mail: gasser@ariel.ucs.unimelb.edu.au</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>Present address: Macfarlane Burnet Centre for Medical Research, P.O. Box 254, Fairfield, Victoria 3078, Australia.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="14" month="5" year="1997"></ce:date-received><ce:date-revised day="23" month="7" year="1997"></ce:date-revised><ce:date-accepted day="28" month="7" year="1997"></ce:date-accepted><ce:abstract class="author"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0040">Twenty-four species of parasitic nematode (order Strongylida) from sheep, goats, cattle or pigs were characterised using a polymerase chain reaction-linked restriction fragment length polymorphism technique (PCR-RFLP). The ribosomal (r)DNA region spanning the first internal transcribed spacer (ITS-1), 5.8S rRNA gene and the second internal transcribed spacer (ITS-2) (designated ITS) was amplified from genomic DNA by polymerase chain reaction (PCR), digested separately with four restriction endonucleases (<ce:italic>Rsa</ce:italic>I, <ce:italic>Hinf</ce:italic>I, <ce:italic>Dra</ce:italic>I or <ce:italic>Nla</ce:italic>III) and the fragments separated by agarose gel electrophoresis. The PCR products amplified from all species appeared as a single band of ∼870 bp in size, except for <ce:italic>Ostertagia ostertagi</ce:italic> whose product was ∼1250 bp. The PCR-RFLP analysis of ITS revealed characteristic restriction patterns for all species, except for <ce:italic>C. surnabada</ce:italic> and <ce:italic>C. oncophora</ce:italic> which had identical patterns. The study demonstrated that ITS contains useful genetic markers for the identification of a range of strongylid nematodes of livestock. These markers should be of use in specific PCR assays for the identification of developmental stages of the parasites where morphological characters are unreliable.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Ribosomal DNA</ce:text></ce:keyword><ce:keyword><ce:text>Internal transcribed spacers</ce:text></ce:keyword><ce:keyword><ce:text>PCR-RFLP</ce:text></ce:keyword><ce:keyword><ce:text>Genetic markers</ce:text></ce:keyword><ce:keyword><ce:text>Species identification</ce:text></ce:keyword><ce:keyword><ce:text>Parasitic nematodes of livestock</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Genetic markers for strongylid nematodes of livestock defined by PCR-based restriction analysis of spacer rDNA</title></titleInfo><name type="personal"><namePart type="given">L.A</namePart><namePart type="family">Newton</namePart><affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</affiliation><affiliation>1Present address: Macfarlane Burnet Centre for Medical Research, P.O. Box 254, Fairfield, Victoria 3078, Australia.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N.B</namePart><namePart type="family">Chilton</namePart><affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I</namePart><namePart type="family">Beveridge</namePart><affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H</namePart><namePart type="family">Hoste</namePart><affiliation>Institut National de la Recherche Agronomique, Station de Pathologie Aviaire et de Parasitologie, Centre de Tours, 37380 Nouzilly, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P</namePart><namePart type="family">Nansen</namePart><affiliation>Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.B</namePart><namePart type="family">Gasser</namePart><affiliation>Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia</affiliation><affiliation>Corresponding author. E-mail: gasser@ariel.ucs.unimelb.edu.au</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1998</dateIssued><dateModified encoding="w3cdtf">1997-07-23</dateModified><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Twenty-four species of parasitic nematode (order Strongylida) from sheep, goats, cattle or pigs were characterised using a polymerase chain reaction-linked restriction fragment length polymorphism technique (PCR-RFLP). The ribosomal (r)DNA region spanning the first internal transcribed spacer (ITS-1), 5.8S rRNA gene and the second internal transcribed spacer (ITS-2) (designated ITS) was amplified from genomic DNA by polymerase chain reaction (PCR), digested separately with four restriction endonucleases (RsaI, HinfI, DraI or NlaIII) and the fragments separated by agarose gel electrophoresis. The PCR products amplified from all species appeared as a single band of ∼870 bp in size, except for Ostertagia ostertagi whose product was ∼1250 bp. The PCR-RFLP analysis of ITS revealed characteristic restriction patterns for all species, except for C. surnabada and C. oncophora which had identical patterns. The study demonstrated that ITS contains useful genetic markers for the identification of a range of strongylid nematodes of livestock. These markers should be of use in specific PCR assays for the identification of developmental stages of the parasites where morphological characters are unreliable.</abstract><note type="content">Fig. 1: Gel showing ITS PCR products of Ostertagia erschowi (lane 1), O. leptospicularis (lane 2) and the ∼1250 bp product of O. ostertagi (lane 3). The ITS PCR products of all species examined in this study, except O. ostertagi, were ∼870 bp in size.</note><note type="content">Fig. 2: Example of an agarose gel showing a lack of variation in the ITS restriction patterns (using endonucleases HinfI and RsaI) among different samples of Haemonchus contortus (Hc9, Hc10, Hc13, Hc15, Hc16, Hc17, Hc21, Hc22, Hc24, Hc26, Hc31 and Hc33, lanes 1–12, respectively). ΦX174-HaeIII molecular weight markers (Promega) represented with m.</note><note type="content">Fig. 3: Examples of the PCR-RFLP analysis of ITS rDNA with the endonucleases RsaI and NlaIII showing interspecific differences in restriction patterns. Lanes: Teladorsagia circumcincta (1); Ostertagia ostertagi (2); O. leptospicularis (3); O. erschowi (4); Hyostrongylus rubidus (5); Oesophagostomum radiatum (6); Oe. venulosum (7); Oe. columbianum (8); Oe. dentatum (9); Oe. quadrispinulatum (10); Nematodirus spathiger (11); N. filicollis (12); N. helvetianus (13); and N. battus (14). Ordinate values indicate approximate molecular weight in base pairs. A mix of ΦX174-HaeIII and pGEM™ molecular weight markers (Promega) represented with M.</note><note type="content">Fig. 4: Schematic representation of the ITS rDNA restriction patterns produced for 24 species of strongylid nematodes of livestock (sheep/goat, cattle or pig). Individual boxes contain patterns produced for restriction endonucleases DraI, NlaIII, HinfI and RsaI. Molecular weights in bp (left). Size of undigested PCR products was ∼870 bp for all species except O. ostertagi (∼1250 bp). Cooperia oncophora and C. surnabada. (represented by C) could not be differentiated by their restriction pattern using any of the four endonucleases. Restriction patterns that were representative for multiple species are designated with `G': G1: Haemonchus contortus, Nematodirus battus, N. filicollis, N. spathiger, Oesophagostomum columbianum and Chabertia ovina. G2: Trichostrongylus axei, T. colubriformis and T. vitrinus. G3: T. axei and T. colubriformis. G4: T. axei and T. vitrinus. G5: N. spathiger and N. helvetianus. G6: Oesophagostomum dentatum and Oe. quadrispinulatum. G7: Haemonchus placei, N. helvetianus and N. spathiger. G8: Cooperia oncophora, C. surnabada and C. punctata. ΦX174-HaeIII molecular weight markers (Promega) represented with m.</note><note type="content">Table 1: Parasite material</note><subject lang="en"><genre>Keywords</genre><topic>Ribosomal DNA</topic><topic>Internal transcribed spacers</topic><topic>PCR-RFLP</topic><topic>Genetic markers</topic><topic>Species identification</topic><topic>Parasitic nematodes of livestock</topic></subject><relatedItem type="host"><titleInfo><title>Acta Tropica</title></titleInfo><titleInfo type="abbreviated"><title>ACTROP</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">199803</dateIssued></originInfo><identifier type="ISSN">0001-706X</identifier><identifier type="PII">S0001-706X(00)X0033-2</identifier><part><date>199803</date><detail type="volume"><number>69</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>88</end></extent><extent unit="pages"><start>1</start><end>15</end></extent></part></relatedItem><identifier type="istex">5321BECAD8BC24065B1F953F89E0E61A65178255</identifier><identifier type="ark">ark:/67375/6H6-C1RCDBM5-J</identifier><identifier type="DOI">10.1016/S0001-706X(97)00105-8</identifier><identifier type="PII">S0001-706X(97)00105-8</identifier><identifier type="ArticleID">752</identifier><accessCondition type="use and reproduction" contentType="copyright">©1998 Elsevier Science B.V.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Elsevier Science B.V., ©1998</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/5321BECAD8BC24065B1F953F89E0E61A65178255/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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