Auto-fluorescence emitted from the cell residues preserved in human tissues of medieval Korean mummies
Identifieur interne : 000288 ( Pmc/Corpus ); précédent : 000287; suivant : 000289Auto-fluorescence emitted from the cell residues preserved in human tissues of medieval Korean mummies
Auteurs : Do-Seon Lim ; Chang Seok Oh ; Sang Jun Lee ; Dong Hoon ShinSource :
- Journal of Anatomy [ 0021-8782 ] ; 2010.
Abstract
As a significant association has been established between residual ancient DNA (aDNA) and histological preservation, the morphological identification or confirmation of preserved cell residue in ancient tissues would greatly facilitate aDNA studies and enhance the definitiveness of their conclusions. However, morphological differentiation of cell residue from other tissue structures has always been difficult, even for experienced histologists, due to the severe degradation of cells over long burial durations. In the present study, using a fluorescence microscopy equipped with a specific type of filter set (excitation filter, 510–550 nm; dichroic mirror, 570 nm; emission filter, ∼590 nm), we found that certain structures in well-preserved mummified tissues emitted auto-fluorescence. Those structures were actually cell residues (e.g. fragmented DNA), laser capture microdissection and Quantifiler kit analysis having shown that preservation of nuclear DNA correlates with auto-fluorescence emission in laser capture microdissection-captured areas. Detection of auto-fluorescence could be an effective means of identifying cell residues in ancient tissue, enabling selection of the well-preserved samples necessary in successful aDNA studies.
Url:
DOI: 10.1111/j.1469-7580.2010.01240.x
PubMed: 20456521
PubMed Central: 2913013
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PMC:2913013Le document en format XML
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<series><title level="j">Journal of Anatomy</title>
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<front><div type="abstract" xml:lang="en"><p>As a significant association has been established between residual ancient DNA (aDNA) and histological preservation, the morphological identification or confirmation of preserved cell residue in ancient tissues would greatly facilitate aDNA studies and enhance the definitiveness of their conclusions. However, morphological differentiation of cell residue from other tissue structures has always been difficult, even for experienced histologists, due to the severe degradation of cells over long burial durations. In the present study, using a fluorescence microscopy equipped with a specific type of filter set (excitation filter, 510–550 nm; dichroic mirror, 570 nm; emission filter, ∼590 nm), we found that certain structures in well-preserved mummified tissues emitted auto-fluorescence. Those structures were actually cell residues (e.g. fragmented DNA), laser capture microdissection and Quantifiler kit analysis having shown that preservation of nuclear DNA correlates with auto-fluorescence emission in laser capture microdissection-captured areas. Detection of auto-fluorescence could be an effective means of identifying cell residues in ancient tissue, enabling selection of the well-preserved samples necessary in successful aDNA studies.</p>
</div>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Anat</journal-id>
<journal-id journal-id-type="publisher-id">joa</journal-id>
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<issn pub-type="ppub">0021-8782</issn>
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<article-id pub-id-type="doi">10.1111/j.1469-7580.2010.01240.x</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Articles</subject>
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<title-group><article-title>Auto-fluorescence emitted from the cell residues preserved in human tissues of medieval Korean mummies</article-title>
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<contrib-group><contrib contrib-type="author"><name><surname>Lim</surname>
<given-names>Do-Seon</given-names>
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<xref ref-type="aff" rid="au1">1</xref>
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<contrib contrib-type="author"><name><surname>Oh</surname>
<given-names>Chang Seok</given-names>
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<xref ref-type="aff" rid="au2">2</xref>
<xref ref-type="aff" rid="au3">3</xref>
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<contrib contrib-type="author"><name><surname>Lee</surname>
<given-names>Sang Jun</given-names>
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<xref ref-type="aff" rid="au2">2</xref>
<xref ref-type="aff" rid="au3">3</xref>
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<contrib contrib-type="author"><name><surname>Shin</surname>
<given-names>Dong Hoon</given-names>
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<xref ref-type="aff" rid="au3">3</xref>
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<aff id="au1"><label>1</label>
<institution>Department of Dental Hygiene, Eulji University</institution>
<addr-line>Sungnam, Korea</addr-line>
</aff>
<aff id="au2"><label>2</label>
<institution>Department of Anatomy, Seoul National University College of Medicine</institution>
<addr-line>Seoul, Korea</addr-line>
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<aff id="au3"><label>3</label>
<institution>Institute of Forensic Medicine, Seoul National University College of Medicine</institution>
<addr-line>Seoul, Korea</addr-line>
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<author-notes><corresp id="cor1">Dong Hoon Shin, Department of Anatomy, Seoul National University College of Medicine, Seoul, Korea. T: +82 2 740 8203; E: <email>drdoogi@snu.ac.kr</email>
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<pub-date pub-type="ppub"><month>7</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="epub"><day>27</day>
<month>4</month>
<year>2010</year>
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<volume>217</volume>
<issue>1</issue>
<fpage>67</fpage>
<lpage>75</lpage>
<history><date date-type="accepted"><day>22</day>
<month>3</month>
<year>2010</year>
</date>
</history>
<permissions><copyright-statement>Journal compilation © 2010 Anatomical Society of Great Britain and Ireland</copyright-statement>
</permissions>
<abstract><p>As a significant association has been established between residual ancient DNA (aDNA) and histological preservation, the morphological identification or confirmation of preserved cell residue in ancient tissues would greatly facilitate aDNA studies and enhance the definitiveness of their conclusions. However, morphological differentiation of cell residue from other tissue structures has always been difficult, even for experienced histologists, due to the severe degradation of cells over long burial durations. In the present study, using a fluorescence microscopy equipped with a specific type of filter set (excitation filter, 510–550 nm; dichroic mirror, 570 nm; emission filter, ∼590 nm), we found that certain structures in well-preserved mummified tissues emitted auto-fluorescence. Those structures were actually cell residues (e.g. fragmented DNA), laser capture microdissection and Quantifiler kit analysis having shown that preservation of nuclear DNA correlates with auto-fluorescence emission in laser capture microdissection-captured areas. Detection of auto-fluorescence could be an effective means of identifying cell residues in ancient tissue, enabling selection of the well-preserved samples necessary in successful aDNA studies.</p>
</abstract>
<kwd-group><kwd>auto-fluorescence</kwd>
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<kwd>fluorescence microscope</kwd>
<kwd>Korea</kwd>
<kwd>mummified tissue</kwd>
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