Mageeibacillus indolicus gen. nov., sp. nov: A novel bacterium isolated from the female genital tract
Identifieur interne : 004319 ( Ncbi/Checkpoint ); précédent : 004318; suivant : 004320Mageeibacillus indolicus gen. nov., sp. nov: A novel bacterium isolated from the female genital tract
Auteurs : Michele N. Austin [États-Unis] ; Lorna K. Rabe [États-Unis] ; Sujatha Srinivasan [États-Unis] ; David N. Fredricks [États-Unis] ; Harold C. Wiesenfeld [États-Unis] ; Sharon L. Hillier [États-Unis]Source :
- Anaerobe [ 1075-9964 ] ; 2014.
Descripteurs français
- KwdFr :
- ADN bactérien, ARN ribosomique 16S (génétique), Données de séquences moléculaires, Femelle, Firmicutes (), Firmicutes (isolement et purification), Firmicutes (métabolisme), Humains, Infections bactériennes à Gram positif (microbiologie), Maladies de l'appareil génital féminin (microbiologie), Phylogénie, Système génital de la femme (microbiologie), Vaginose bactérienne (microbiologie).
- MESH :
- génétique : ARN ribosomique 16S.
- isolement et purification : Firmicutes.
- microbiologie : Infections bactériennes à Gram positif, Maladies de l'appareil génital féminin, Système génital de la femme, Vaginose bactérienne.
- métabolisme : Firmicutes.
- ADN bactérien, Données de séquences moléculaires, Femelle, Firmicutes, Humains, Phylogénie.
English descriptors
- KwdEn :
- DNA, Bacterial, Female, Firmicutes (classification), Firmicutes (drug effects), Firmicutes (isolation & purification), Firmicutes (metabolism), Genital Diseases, Female (microbiology), Genitalia, Female (microbiology), Gram-Positive Bacterial Infections (microbiology), Humans, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S (genetics), Vaginosis, Bacterial (microbiology).
- MESH :
- chemical , genetics : RNA, Ribosomal, 16S.
- chemical : DNA, Bacterial.
- classification : Firmicutes.
- drug effects : Firmicutes.
- isolation & purification : Firmicutes.
- metabolism : Firmicutes.
- microbiology : Genital Diseases, Female, Genitalia, Female, Gram-Positive Bacterial Infections, Vaginosis, Bacterial.
- Female, Humans, Molecular Sequence Data, Phylogeny.
Abstract
Three isolates of a bacterium recovered from human endometrium using conventional culture methods were characterized biochemically and subjected to 16S rRNA gene sequencing and phylogenetic analysis. Isolates were non-motile, obligately anaerobic, non-spore forming, asaccharolytic, non-cellulolytic, indole positive, Gram positive rods. Cell wall fatty acid profiling revealed C14:0, C16:0, C18:2 ω6, 9c, C18:1 ω9c and C18:0 to be the major fatty acid composition. The DNA mol % G+C was determined to be 44.2%. 16S rRNA gene sequence analysis revealed only 91% sequence similarity with the closest cultivated bacterial isolate,
Url:
DOI: 10.1016/j.anaerobe.2014.12.003
PubMed: 25482717
PubMed Central: 4385425
Affiliations:
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PMC:4385425Le document en format XML
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gen. nov., sp. nov: A novel bacterium isolated from the female genital tract</title>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>Mageeibacillus indolicus</italic>
gen. nov., sp. nov: A novel bacterium isolated from the female genital tract</title>
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<term>Female</term>
<term>Firmicutes (classification)</term>
<term>Firmicutes (drug effects)</term>
<term>Firmicutes (isolation & purification)</term>
<term>Firmicutes (metabolism)</term>
<term>Genital Diseases, Female (microbiology)</term>
<term>Genitalia, Female (microbiology)</term>
<term>Gram-Positive Bacterial Infections (microbiology)</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>RNA, Ribosomal, 16S (genetics)</term>
<term>Vaginosis, Bacterial (microbiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien</term>
<term>ARN ribosomique 16S (génétique)</term>
<term>Données de séquences moléculaires</term>
<term>Femelle</term>
<term>Firmicutes ()</term>
<term>Firmicutes (isolement et purification)</term>
<term>Firmicutes (métabolisme)</term>
<term>Humains</term>
<term>Infections bactériennes à Gram positif (microbiologie)</term>
<term>Maladies de l'appareil génital féminin (microbiologie)</term>
<term>Phylogénie</term>
<term>Système génital de la femme (microbiologie)</term>
<term>Vaginose bactérienne (microbiologie)</term>
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</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Bacterial</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Firmicutes</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Firmicutes</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN ribosomique 16S</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Firmicutes</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Firmicutes</term>
</keywords>
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</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Infections bactériennes à Gram positif</term>
<term>Maladies de l'appareil génital féminin</term>
<term>Système génital de la femme</term>
<term>Vaginose bactérienne</term>
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<term>Genitalia, Female</term>
<term>Gram-Positive Bacterial Infections</term>
<term>Vaginosis, Bacterial</term>
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<front><div type="abstract" xml:lang="en"><p id="P1">Three isolates of a bacterium recovered from human endometrium using conventional culture methods were characterized biochemically and subjected to 16S rRNA gene sequencing and phylogenetic analysis. Isolates were non-motile, obligately anaerobic, non-spore forming, asaccharolytic, non-cellulolytic, indole positive, Gram positive rods. Cell wall fatty acid profiling revealed C<sub>14:0</sub>
, C<sub>16:0</sub>
, C<sub>18:2 ω6, 9c</sub>
, C<sub>18:1 ω9c</sub>
and C<sub>18:0</sub>
to be the major fatty acid composition. The DNA mol % G+C was determined to be 44.2%. 16S rRNA gene sequence analysis revealed only 91% sequence similarity with the closest cultivated bacterial isolate, <italic>Saccharofermentans acetigenes</italic>
. Based on genotypic and phenotypic data, all three isolates are considered to be members of the same species and data suggest it represents a novel genus and species in the order <italic>Clostridiales</italic>
with an association with <italic>Clostridium</italic>
rRNA cluster III within the family <italic>Ruminococcaceae</italic>
. We propose the name, <italic>Mageeibacillus indolicus</italic>
gen. nov., sp. nov. The type strain is BAA-2120<sup>T</sup>
and CCUG 59143<sup>T</sup>
.</p>
</div>
</front>
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<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Pennsylvanie</li>
<li>Washington (État)</li>
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<tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Austin, Michele N" sort="Austin, Michele N" uniqKey="Austin M" first="Michele N." last="Austin">Michele N. Austin</name>
</region>
<name sortKey="Fredricks, David N" sort="Fredricks, David N" uniqKey="Fredricks D" first="David N." last="Fredricks">David N. Fredricks</name>
<name sortKey="Hillier, Sharon L" sort="Hillier, Sharon L" uniqKey="Hillier S" first="Sharon L." last="Hillier">Sharon L. Hillier</name>
<name sortKey="Hillier, Sharon L" sort="Hillier, Sharon L" uniqKey="Hillier S" first="Sharon L." last="Hillier">Sharon L. Hillier</name>
<name sortKey="Rabe, Lorna K" sort="Rabe, Lorna K" uniqKey="Rabe L" first="Lorna K." last="Rabe">Lorna K. Rabe</name>
<name sortKey="Srinivasan, Sujatha" sort="Srinivasan, Sujatha" uniqKey="Srinivasan S" first="Sujatha" last="Srinivasan">Sujatha Srinivasan</name>
<name sortKey="Wiesenfeld, Harold C" sort="Wiesenfeld, Harold C" uniqKey="Wiesenfeld H" first="Harold C." last="Wiesenfeld">Harold C. Wiesenfeld</name>
<name sortKey="Wiesenfeld, Harold C" sort="Wiesenfeld, Harold C" uniqKey="Wiesenfeld H" first="Harold C." last="Wiesenfeld">Harold C. Wiesenfeld</name>
</country>
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