PittsburghV1, Ncbi, Checkpoint, bibRecord, 004319

Mageeibacillus indolicus gen. nov., sp. nov: A novel bacterium isolated from the female genital tract

Identifieur interne : 004319 ( Ncbi/Checkpoint ); précédent : 004318; suivant : 004320

Mageeibacillus indolicus gen. nov., sp. nov: A novel bacterium isolated from the female genital tract

Auteurs : Michele N. Austin [États-Unis] ; Lorna K. Rabe [États-Unis] ; Sujatha Srinivasan [États-Unis] ; David N. Fredricks [États-Unis] ; Harold C. Wiesenfeld [États-Unis] ; Sharon L. Hillier [États-Unis]

Source :

Anaerobe [ 1075-9964 ] ; 2014.

RBID : PMC:4385425

Descripteurs français

KwdFr :
- ADN bactérien, ARN ribosomique 16S (génétique), Données de séquences moléculaires, Femelle, Firmicutes (), Firmicutes (isolement et purification), Firmicutes (métabolisme), Humains, Infections bactériennes à Gram positif (microbiologie), Maladies de l'appareil génital féminin (microbiologie), Phylogénie, Système génital de la femme (microbiologie), Vaginose bactérienne (microbiologie).
MESH :
- génétique : ARN ribosomique 16S.
- isolement et purification : Firmicutes.
- microbiologie : Infections bactériennes à Gram positif, Maladies de l'appareil génital féminin, Système génital de la femme, Vaginose bactérienne.
- métabolisme : Firmicutes.
- ADN bactérien, Données de séquences moléculaires, Femelle, Firmicutes, Humains, Phylogénie.

English descriptors

KwdEn :
- DNA, Bacterial, Female, Firmicutes (classification), Firmicutes (drug effects), Firmicutes (isolation & purification), Firmicutes (metabolism), Genital Diseases, Female (microbiology), Genitalia, Female (microbiology), Gram-Positive Bacterial Infections (microbiology), Humans, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S (genetics), Vaginosis, Bacterial (microbiology).
MESH :
- chemical , genetics : RNA, Ribosomal, 16S.
- chemical : DNA, Bacterial.
- classification : Firmicutes.
- drug effects : Firmicutes.
- isolation & purification : Firmicutes.
- metabolism : Firmicutes.
- microbiology : Genital Diseases, Female, Genitalia, Female, Gram-Positive Bacterial Infections, Vaginosis, Bacterial.
- Female, Humans, Molecular Sequence Data, Phylogeny.

Abstract

Three isolates of a bacterium recovered from human endometrium using conventional culture methods were characterized biochemically and subjected to 16S rRNA gene sequencing and phylogenetic analysis. Isolates were non-motile, obligately anaerobic, non-spore forming, asaccharolytic, non-cellulolytic, indole positive, Gram positive rods. Cell wall fatty acid profiling revealed C_14:0, C_16:0, C_{18:2 ω6, 9c}, C_{18:1 ω9c} and C_18:0 to be the major fatty acid composition. The DNA mol % G+C was determined to be 44.2%. 16S rRNA gene sequence analysis revealed only 91% sequence similarity with the closest cultivated bacterial isolate, Saccharofermentans acetigenes. Based on genotypic and phenotypic data, all three isolates are considered to be members of the same species and data suggest it represents a novel genus and species in the order Clostridiales with an association with Clostridium rRNA cluster III within the family Ruminococcaceae. We propose the name, Mageeibacillus indolicus gen. nov., sp. nov. The type strain is BAA-2120^T and CCUG 59143^T.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4385425

DOI: 10.1016/j.anaerobe.2014.12.003
PubMed: 25482717
PubMed Central: 4385425

Affiliations:

Links to Exploration step

PMC:4385425

Le document en format XML

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 gen. nov., sp. nov: A novel bacterium isolated from the female genital tract</title>
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 gen. nov., sp. nov: A novel bacterium isolated from the female genital tract</title>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Firmicutes</term>
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<front><div type="abstract" xml:lang="en"><p id="P1">Three isolates of a bacterium recovered from human endometrium using conventional culture methods were characterized biochemically and subjected to 16S rRNA gene sequencing and phylogenetic analysis. Isolates were non-motile, obligately anaerobic, non-spore forming, asaccharolytic, non-cellulolytic, indole positive, Gram positive rods. Cell wall fatty acid profiling revealed C<sub>14:0</sub>
, C<sub>16:0</sub>
, C<sub>18:2 ω6, 9c</sub>
, C<sub>18:1 ω9c</sub>
 and C<sub>18:0</sub>
 to be the major fatty acid composition. The DNA mol % G+C was determined to be 44.2%. 16S rRNA gene sequence analysis revealed only 91% sequence similarity with the closest cultivated bacterial isolate, <italic>Saccharofermentans acetigenes</italic>
. Based on genotypic and phenotypic data, all three isolates are considered to be members of the same species and data suggest it represents a novel genus and species in the order <italic>Clostridiales</italic>
 with an association with <italic>Clostridium</italic>
 rRNA cluster III within the family <italic>Ruminococcaceae</italic>
. We propose the name, <italic>Mageeibacillus indolicus</italic>
 gen. nov., sp. nov. The type strain is BAA-2120<sup>T</sup>
 and CCUG 59143<sup>T</sup>
.</p>
</div>
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<name sortKey="Hillier, Sharon L" sort="Hillier, Sharon L" uniqKey="Hillier S" first="Sharon L." last="Hillier">Sharon L. Hillier</name>
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Mageeibacillus indolicus gen. nov., sp. nov: A novel bacterium isolated from the female genital tract

Mageeibacillus indolicus gen. nov., sp. nov: A novel bacterium isolated from the female genital tract

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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