The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck).
Identifieur interne : 000E29 ( PubMed/Corpus ); précédent : 000E28; suivant : 000E30The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck).
Auteurs : R L Boscariol ; W A B. Almeida ; M T V C. Derbyshire ; F A A. Mourão Filho ; B M J. MendesSource :
- Plant cell reports [ 0721-7714 ] ; 2003.
English descriptors
- KwdEn :
- Citrus sinensis (drug effects), Citrus sinensis (genetics), Citrus sinensis (growth & development), Culture Techniques, Fructose (pharmacology), Gene Expression Regulation, Enzymologic (drug effects), Genetic Markers (genetics), Mannose (pharmacology), Mannose-6-Phosphate Isomerase (genetics), Mannose-6-Phosphate Isomerase (metabolism), Plants, Genetically Modified (drug effects), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Sucrose (pharmacology).
- MESH :
- chemical , genetics : Genetic Markers, Mannose-6-Phosphate Isomerase.
- chemical , metabolism : Mannose-6-Phosphate Isomerase.
- chemical , pharmacology : Fructose, Mannose, Sucrose.
- drug effects : Citrus sinensis, Gene Expression Regulation, Enzymologic, Plants, Genetically Modified.
- genetics : Citrus sinensis, Plants, Genetically Modified.
- growth & development : Citrus sinensis, Plants, Genetically Modified.
- Culture Techniques.
Abstract
A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides.
DOI: 10.1007/s00299-003-0654-1
PubMed: 12879258
Links to Exploration step
pubmed:12879258Le document en format XML
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Mourão Filho</name></author><author><name sortKey="Mendes, B M J" sort="Mendes, B M J" uniqKey="Mendes B" first="B M J" last="Mendes">B M J. Mendes</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12879258</idno><idno type="pmid">12879258</idno><idno type="doi">10.1007/s00299-003-0654-1</idno><idno type="wicri:Area/PubMed/Corpus">000E29</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck).</title><author><name sortKey="Boscariol, R L" sort="Boscariol, R L" uniqKey="Boscariol R" first="R L" last="Boscariol">R L Boscariol</name><affiliation><nlm:affiliation>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13 400-970 São Paulo, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Almeida, W A B" sort="Almeida, W A B" uniqKey="Almeida W" first="W A B" last="Almeida">W A B. Almeida</name></author><author><name sortKey="Derbyshire, M T V C" sort="Derbyshire, M T V C" uniqKey="Derbyshire M" first="M T V C" last="Derbyshire">M T V C. Derbyshire</name></author><author><name sortKey="Mourao Filho, F A A" sort="Mourao Filho, F A A" uniqKey="Mourao Filho F" first="F A A" last="Mourão Filho">F A A. Mourão Filho</name></author><author><name sortKey="Mendes, B M J" sort="Mendes, B M J" uniqKey="Mendes B" first="B M J" last="Mendes">B M J. Mendes</name></author></analytic><series><title level="j">Plant cell reports</title><idno type="ISSN">0721-7714</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus sinensis (drug effects)</term><term>Citrus sinensis (genetics)</term><term>Citrus sinensis (growth & development)</term><term>Culture Techniques</term><term>Fructose (pharmacology)</term><term>Gene Expression Regulation, Enzymologic (drug effects)</term><term>Genetic Markers (genetics)</term><term>Mannose (pharmacology)</term><term>Mannose-6-Phosphate Isomerase (genetics)</term><term>Mannose-6-Phosphate Isomerase (metabolism)</term><term>Plants, Genetically Modified (drug effects)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Sucrose (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Genetic Markers</term><term>Mannose-6-Phosphate Isomerase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mannose-6-Phosphate Isomerase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Fructose</term><term>Mannose</term><term>Sucrose</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Citrus sinensis</term><term>Gene Expression Regulation, Enzymologic</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Citrus sinensis</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Culture Techniques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12879258</PMID><DateCreated><Year>2003</Year><Month>9</Month><Day>23</Day></DateCreated><DateCompleted><Year>2004</Year><Month>02</Month><Day>09</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0721-7714</ISSN><JournalIssue CitedMedium="Print"><Volume>22</Volume><Issue>2</Issue><PubDate><Year>2003</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep.</ISOAbbreviation></Journal><ArticleTitle>The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck).</ArticleTitle><Pagination><MedlinePgn>122-8</MedlinePgn></Pagination><Abstract><AbstractText>A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Boscariol</LastName><ForeName>R L</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13 400-970 São Paulo, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Almeida</LastName><ForeName>W A B</ForeName><Initials>WA</Initials></Author><Author ValidYN="Y"><LastName>Derbyshire</LastName><ForeName>M T V C</ForeName><Initials>MT</Initials></Author><Author ValidYN="Y"><LastName>Mourão Filho</LastName><ForeName>F A A</ForeName><Initials>FA</Initials></Author><Author ValidYN="Y"><LastName>Mendes</LastName><ForeName>B M J</ForeName><Initials>BM</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2003</Year><Month>Jul</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical><Chemical><RegistryNumber>30237-26-4</RegistryNumber><NameOfSubstance UI="D005632">Fructose</NameOfSubstance></Chemical><Chemical><RegistryNumber>57-50-1</RegistryNumber><NameOfSubstance UI="D013395">Sucrose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 5.3.1.8</RegistryNumber><NameOfSubstance UI="D008359">Mannose-6-Phosphate Isomerase</NameOfSubstance></Chemical><Chemical><RegistryNumber>PHA4727WTP</RegistryNumber><NameOfSubstance UI="D008358">Mannose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046508" MajorTopicYN="N">Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005632" MajorTopicYN="N">Fructose</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="N">Gene Expression Regulation, Enzymologic</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008358" MajorTopicYN="N">Mannose</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008359" MajorTopicYN="N">Mannose-6-Phosphate Isomerase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013395" MajorTopicYN="N">Sucrose</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2003</Year><Month>Nov</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2003</Year><Month>May</Month><Day>2</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2003</Year><Month>May</Month><Day>5</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>7</Month><Day>25</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>2</Month><Day>11</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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