Structure-Function Analysis of the HrpB2-HrcU Interaction in the Xanthomonas citri Type III Secretion System
Identifieur interne : 001216 ( Pmc/Curation ); précédent : 001215; suivant : 001217Structure-Function Analysis of the HrpB2-HrcU Interaction in the Xanthomonas citri Type III Secretion System
Auteurs : Paola A. Cappelletti [Brésil] ; Rafael Freitas Dos Santos [Brésil] ; Alexandre M. Do Amaral [Brésil] ; Rafael Augusto Homem [Brésil] ; Thaís Dos Santos Souza [Brésil] ; Marcos A. Machado [Brésil] ; Chuck S. Farah [Brésil]Source :
- PLoS ONE [ 1932-6203 ] ; 2011.
Abstract
Bacterial type III secretion systems deliver protein virulence factors to host cells. Here we characterize the interaction between HrpB2, a small protein secreted by the
Url:
DOI: 10.1371/journal.pone.0017614
PubMed: 21408079
PubMed Central: 3052322
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PMC:3052322Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Structure-Function Analysis of the HrpB2-HrcU Interaction in the <italic>Xanthomonas citri</italic>
Type III Secretion System</title>
<author><name sortKey="Cappelletti, Paola A" sort="Cappelletti, Paola A" uniqKey="Cappelletti P" first="Paola A." last="Cappelletti">Paola A. Cappelletti</name>
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<author><name sortKey="Dos Santos, Rafael Freitas" sort="Dos Santos, Rafael Freitas" uniqKey="Dos Santos R" first="Rafael Freitas" last="Dos Santos">Rafael Freitas Dos Santos</name>
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<author><name sortKey="Homem, Rafael Augusto" sort="Homem, Rafael Augusto" uniqKey="Homem R" first="Rafael Augusto" last="Homem">Rafael Augusto Homem</name>
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<author><name sortKey="Dos Santos Souza, Thais" sort="Dos Santos Souza, Thais" uniqKey="Dos Santos Souza T" first="Thaís" last="Dos Santos Souza">Thaís Dos Santos Souza</name>
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<author><name sortKey="Farah, Chuck S" sort="Farah, Chuck S" uniqKey="Farah C" first="Chuck S." last="Farah">Chuck S. Farah</name>
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<series><title level="j">PLoS ONE</title>
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<front><div type="abstract" xml:lang="en"><p>Bacterial type III secretion systems deliver protein virulence factors to host cells. Here we characterize the interaction between HrpB2, a small protein secreted by the <italic>Xanthomonas citri</italic>
subsp. citri type III secretion system, and the cytosolic domain of the inner membrane protein HrcU, a paralog of the flagellar protein FlhB. We show that a recombinant fragment corresponding to the C-terminal cytosolic domain of HrcU produced in <italic>E. coli</italic>
suffers cleavage within a conserved Asn264-Pro265-Thr266-His267 (NPTH) sequence. A recombinant HrcU cytosolic domain with N264A, P265A, T266A mutations at the cleavage site (HrcU<sub>AAAH</sub>
) was not cleaved and interacted with HrpB2. Furthermore, a polypeptide corresponding to the sequence following the NPTH cleavage site also interacted with HrpB2 indicating that the site for interaction is located after the NPTH site. Non-polar deletion mutants of the <italic>hrcU</italic>
and <italic>hrpB2</italic>
genes resulted in a total loss of pathogenicity in susceptible citrus plants and disease symptoms could be recovered by expression of HrpB2 and HrcU from extrachromossomal plasmids. Complementation of the <italic>ΔhrcU</italic>
mutant with HrcU<sub>AAAH</sub>
produced canker lesions similar to those observed when complemented with wild-type HrcU. HrpB2 secretion however, was significantly reduced in the <italic>ΔhrcU</italic>
mutant complemented with HrcU<sub>AAAH,</sub>
suggesting that an intact and cleavable NPTH site in HrcU is necessary for total functionally of T3SS in <italic>X. citri</italic>
subsp. citri. Complementation of the <italic>ΔhrpB2 X. citri</italic>
subsp. citri strain with a series of <italic>hrpB2</italic>
gene mutants revealed that the highly conserved HrpB2 C-terminus is essential for T3SS-dependent development of citrus canker symptoms <italic>in planta</italic>
.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Blocker, A" uniqKey="Blocker A">A Blocker</name>
</author>
<author><name sortKey="Komoriya, K" uniqKey="Komoriya K">K Komoriya</name>
</author>
<author><name sortKey="Aizawa, S" uniqKey="Aizawa S">S Aizawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="He, Sy" uniqKey="He S">SY He</name>
</author>
<author><name sortKey="Nomura, K" uniqKey="Nomura K">K Nomura</name>
</author>
<author><name sortKey="Whittam, Ts" uniqKey="Whittam T">TS Whittam</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Buttner, D" uniqKey="Buttner D">D Buttner</name>
</author>
<author><name sortKey="He, Sy" uniqKey="He S">SY He</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rong, W" uniqKey="Rong W">W Rong</name>
</author>
<author><name sortKey="Feng, F" uniqKey="Feng F">F Feng</name>
</author>
<author><name sortKey="Zhou, J" uniqKey="Zhou J">J Zhou</name>
</author>
<author><name sortKey="He, C" uniqKey="He C">C He</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Alegria, Mc" uniqKey="Alegria M">MC Alegria</name>
</author>
<author><name sortKey="Docena, C" uniqKey="Docena C">C Docena</name>
</author>
<author><name sortKey="Khater, L" uniqKey="Khater L">L Khater</name>
</author>
<author><name sortKey="Ramos, Ch" uniqKey="Ramos C">CH Ramos</name>
</author>
<author><name sortKey="Da Silva, Ac" uniqKey="Da Silva A">AC da Silva</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Buttner, D" uniqKey="Buttner D">D Buttner</name>
</author>
<author><name sortKey="Bonas, U" uniqKey="Bonas U">U Bonas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Da Silva, Ac" uniqKey="Da Silva A">AC da Silva</name>
</author>
<author><name sortKey="Ferro, Ja" uniqKey="Ferro J">JA Ferro</name>
</author>
<author><name sortKey="Reinach, Fc" uniqKey="Reinach F">FC Reinach</name>
</author>
<author><name sortKey="Farah, Cs" uniqKey="Farah C">CS Farah</name>
</author>
<author><name sortKey="Furlan, Lr" uniqKey="Furlan L">LR Furlan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kay, S" uniqKey="Kay S">S Kay</name>
</author>
<author><name sortKey="Bonas, U" uniqKey="Bonas U">U Bonas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Gijsegem, F" uniqKey="Van Gijsegem F">F Van Gijsegem</name>
</author>
<author><name sortKey="Gough, C" uniqKey="Gough C">C Gough</name>
</author>
<author><name sortKey="Zischek, C" uniqKey="Zischek C">C Zischek</name>
</author>
<author><name sortKey="Niqueux, E" uniqKey="Niqueux E">E Niqueux</name>
</author>
<author><name sortKey="Arlat, M" uniqKey="Arlat M">M Arlat</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Furutani, A" uniqKey="Furutani A">A Furutani</name>
</author>
<author><name sortKey="Takaoka, M" uniqKey="Takaoka M">M Takaoka</name>
</author>
<author><name sortKey="Sanada, H" uniqKey="Sanada H">H Sanada</name>
</author>
<author><name sortKey="Noguchi, Y" uniqKey="Noguchi Y">Y Noguchi</name>
</author>
<author><name sortKey="Oku, T" uniqKey="Oku T">T Oku</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marguerettaz, M" uniqKey="Marguerettaz M">M Marguerettaz</name>
</author>
<author><name sortKey="Pieretti, I" uniqKey="Pieretti I">I Pieretti</name>
</author>
<author><name sortKey="Gayral, P" uniqKey="Gayral P">P Gayral</name>
</author>
<author><name sortKey="Puig, J" uniqKey="Puig J">J Puig</name>
</author>
<author><name sortKey="Brin, C" uniqKey="Brin C">C Brin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Szczesny, R" uniqKey="Szczesny R">R Szczesny</name>
</author>
<author><name sortKey="Buttner, D" uniqKey="Buttner D">D Buttner</name>
</author>
<author><name sortKey="Escolar, L" uniqKey="Escolar L">L Escolar</name>
</author>
<author><name sortKey="Schulze, S" uniqKey="Schulze S">S Schulze</name>
</author>
<author><name sortKey="Seiferth, A" uniqKey="Seiferth A">A Seiferth</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Song, C" uniqKey="Song C">C Song</name>
</author>
<author><name sortKey="Yang, B" uniqKey="Yang B">B Yang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moreira, Lm" uniqKey="Moreira L">LM Moreira</name>
</author>
<author><name sortKey="Almeida, Nf" uniqKey="Almeida N">NF Almeida</name>
</author>
<author><name sortKey="Potnis, N" uniqKey="Potnis N">N Potnis</name>
</author>
<author><name sortKey="Digiampietri, La" uniqKey="Digiampietri L">LA Digiampietri</name>
</author>
<author><name sortKey="Adi, Ss" uniqKey="Adi S">SS Adi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pieretti, I" uniqKey="Pieretti I">I Pieretti</name>
</author>
<author><name sortKey="Royer, M" uniqKey="Royer M">M Royer</name>
</author>
<author><name sortKey="Barbe, V" uniqKey="Barbe V">V Barbe</name>
</author>
<author><name sortKey="Carrere, S" uniqKey="Carrere S">S Carrere</name>
</author>
<author><name sortKey="Koebnik, R" uniqKey="Koebnik R">R Koebnik</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brunings, Am" uniqKey="Brunings A">AM Brunings</name>
</author>
<author><name sortKey="Gabriel, Dw" uniqKey="Gabriel D">DW Gabriel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dunger, G" uniqKey="Dunger G">G Dunger</name>
</author>
<author><name sortKey="Arabolaza, Al" uniqKey="Arabolaza A">AL Arabolaza</name>
</author>
<author><name sortKey="Gottig, N" uniqKey="Gottig N">N Gottig</name>
</author>
<author><name sortKey="Orellano, Eg" uniqKey="Orellano E">EG Orellano</name>
</author>
<author><name sortKey="Ottado, J" uniqKey="Ottado J">J Ottado</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Swarup, S" uniqKey="Swarup S">S Swarup</name>
</author>
<author><name sortKey="Yang, Y" uniqKey="Yang Y">Y Yang</name>
</author>
<author><name sortKey="Kingsley, Mt" uniqKey="Kingsley M">MT Kingsley</name>
</author>
<author><name sortKey="Gabriel, Dw" uniqKey="Gabriel D">DW Gabriel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hiroshi, S" uniqKey="Hiroshi S">S Hiroshi</name>
</author>
<author><name sortKey="Takashi, F" uniqKey="Takashi F">F Takashi</name>
</author>
<author><name sortKey="Hiromichi, I" uniqKey="Hiromichi I">I Hiromichi</name>
</author>
<author><name sortKey="Sinji, T" uniqKey="Sinji T">T Sinji</name>
</author>
<author><name sortKey="Katsumi, O" uniqKey="Katsumi O">O Katsumi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rossier, O" uniqKey="Rossier O">O Rossier</name>
</author>
<author><name sortKey="Van Den Ackerveken, G" uniqKey="Van Den Ackerveken G">G Van den Ackerveken</name>
</author>
<author><name sortKey="Bonas, U" uniqKey="Bonas U">U Bonas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lorenz, C" uniqKey="Lorenz C">C Lorenz</name>
</author>
<author><name sortKey="Schulz, S" uniqKey="Schulz S">S Schulz</name>
</author>
<author><name sortKey="Wolsch, T" uniqKey="Wolsch T">T Wolsch</name>
</author>
<author><name sortKey="Rossier, O" uniqKey="Rossier O">O Rossier</name>
</author>
<author><name sortKey="Bonas, U" uniqKey="Bonas U">U Bonas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oh, C" uniqKey="Oh C">C Oh</name>
</author>
<author><name sortKey="Heu, S" uniqKey="Heu S">S Heu</name>
</author>
<author><name sortKey="Yoo, Jy" uniqKey="Yoo J">JY Yoo</name>
</author>
<author><name sortKey="Cho, Y" uniqKey="Cho Y">Y Cho</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bonas, U" uniqKey="Bonas U">U Bonas</name>
</author>
<author><name sortKey="Shulte, R" uniqKey="Shulte R">R Shulte</name>
</author>
<author><name sortKey="Fenselau, S" uniqKey="Fenselau S">S Fenselau</name>
</author>
<author><name sortKey="Minsavage, Gv" uniqKey="Minsavage G">GV Minsavage</name>
</author>
<author><name sortKey="Staskawicz, Bj" uniqKey="Staskawicz B">BJ Staskawicz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Minamino, T" uniqKey="Minamino T">T Minamino</name>
</author>
<author><name sortKey="Macnab, Rm" uniqKey="Macnab R">RM Macnab</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ferris, Hu" uniqKey="Ferris H">HU Ferris</name>
</author>
<author><name sortKey="Furukawa, Y" uniqKey="Furukawa Y">Y Furukawa</name>
</author>
<author><name sortKey="Minamino, T" uniqKey="Minamino T">T Minamino</name>
</author>
<author><name sortKey="Kroetz, Mb" uniqKey="Kroetz M">MB Kroetz</name>
</author>
<author><name sortKey="Kihara, M" uniqKey="Kihara M">M Kihara</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lavander, M" uniqKey="Lavander M">M Lavander</name>
</author>
<author><name sortKey="Sundberg, L" uniqKey="Sundberg L">L Sundberg</name>
</author>
<author><name sortKey="Edqvist, Pj" uniqKey="Edqvist P">PJ Edqvist</name>
</author>
<author><name sortKey="Lloyd, Sa" uniqKey="Lloyd S">SA Lloyd</name>
</author>
<author><name sortKey="Wolf Watz, H" uniqKey="Wolf Watz H">H Wolf-Watz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fraser, Gm" uniqKey="Fraser G">GM Fraser</name>
</author>
<author><name sortKey="Hirano, T" uniqKey="Hirano T">T Hirano</name>
</author>
<author><name sortKey="Ferris, Hu" uniqKey="Ferris H">HU Ferris</name>
</author>
<author><name sortKey="Devgan, Ll" uniqKey="Devgan L">LL Devgan</name>
</author>
<author><name sortKey="Kihara, M" uniqKey="Kihara M">M Kihara</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sorg, I" uniqKey="Sorg I">I Sorg</name>
</author>
<author><name sortKey="Wagner, S" uniqKey="Wagner S">S Wagner</name>
</author>
<author><name sortKey="Amstutz, M" uniqKey="Amstutz M">M Amstutz</name>
</author>
<author><name sortKey="Muller, Sa" uniqKey="Muller S">SA Muller</name>
</author>
<author><name sortKey="Broz, P" uniqKey="Broz P">P Broz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wiesand, U" uniqKey="Wiesand U">U Wiesand</name>
</author>
<author><name sortKey="Sorg, I" uniqKey="Sorg I">I Sorg</name>
</author>
<author><name sortKey="Amstutz, M" uniqKey="Amstutz M">M Amstutz</name>
</author>
<author><name sortKey="Wagner, S" uniqKey="Wagner S">S Wagner</name>
</author>
<author><name sortKey="Van Den Heuvel, J" uniqKey="Van Den Heuvel J">J van den Heuvel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zarivach, R" uniqKey="Zarivach R">R Zarivach</name>
</author>
<author><name sortKey="Deng, W" uniqKey="Deng W">W Deng</name>
</author>
<author><name sortKey="Vuckovic, M" uniqKey="Vuckovic M">M Vuckovic</name>
</author>
<author><name sortKey="Felise, Hb" uniqKey="Felise H">HB Felise</name>
</author>
<author><name sortKey="Nguyen, Hv" uniqKey="Nguyen H">HV Nguyen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Deane, Je" uniqKey="Deane J">JE Deane</name>
</author>
<author><name sortKey="Graham, Sc" uniqKey="Graham S">SC Graham</name>
</author>
<author><name sortKey="Mitchell, Ep" uniqKey="Mitchell E">EP Mitchell</name>
</author>
<author><name sortKey="Flot, D" uniqKey="Flot D">D Flot</name>
</author>
<author><name sortKey="Johnson, S" uniqKey="Johnson S">S Johnson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Allaoui, A" uniqKey="Allaoui A">A Allaoui</name>
</author>
<author><name sortKey="Woestyn, S" uniqKey="Woestyn S">S Woestyn</name>
</author>
<author><name sortKey="Sluiters, C" uniqKey="Sluiters C">C Sluiters</name>
</author>
<author><name sortKey="Cornelis, Gr" uniqKey="Cornelis G">GR Cornelis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Astua Monge, G" uniqKey="Astua Monge G">G Astua-Monge</name>
</author>
<author><name sortKey="Freitas Astua, J" uniqKey="Freitas Astua J">J Freitas-Astua</name>
</author>
<author><name sortKey="Bacocina, G" uniqKey="Bacocina G">G Bacocina</name>
</author>
<author><name sortKey="Roncoletta, J" uniqKey="Roncoletta J">J Roncoletta</name>
</author>
<author><name sortKey="Carvalho, Sa" uniqKey="Carvalho S">SA Carvalho</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fenselau, S" uniqKey="Fenselau S">S Fenselau</name>
</author>
<author><name sortKey="Bonas, U" uniqKey="Bonas U">U Bonas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Koebnik, R" uniqKey="Koebnik R">R Koebnik</name>
</author>
<author><name sortKey="Kruger, A" uniqKey="Kruger A">A Kruger</name>
</author>
<author><name sortKey="Thieme, F" uniqKey="Thieme F">F Thieme</name>
</author>
<author><name sortKey="Urban, A" uniqKey="Urban A">A Urban</name>
</author>
<author><name sortKey="Bonas, U" uniqKey="Bonas U">U Bonas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Aldon, D" uniqKey="Aldon D">D Aldon</name>
</author>
<author><name sortKey="Brito, B" uniqKey="Brito B">B Brito</name>
</author>
<author><name sortKey="Boucher, C" uniqKey="Boucher C">C Boucher</name>
</author>
<author><name sortKey="Genin, S" uniqKey="Genin S">S Genin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brito, B" uniqKey="Brito B">B Brito</name>
</author>
<author><name sortKey="Aldon, D" uniqKey="Aldon D">D Aldon</name>
</author>
<author><name sortKey="Barberis, P" uniqKey="Barberis P">P Barberis</name>
</author>
<author><name sortKey="Boucher, C" uniqKey="Boucher C">C Boucher</name>
</author>
<author><name sortKey="Genin, S" uniqKey="Genin S">S Genin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marenda, M" uniqKey="Marenda M">M Marenda</name>
</author>
<author><name sortKey="Brito, B" uniqKey="Brito B">B Brito</name>
</author>
<author><name sortKey="Callard, D" uniqKey="Callard D">D Callard</name>
</author>
<author><name sortKey="Genin, S" uniqKey="Genin S">S Genin</name>
</author>
<author><name sortKey="Barberis, P" uniqKey="Barberis P">P Barberis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tahara, St" uniqKey="Tahara S">ST Tahara</name>
</author>
<author><name sortKey="Mehta, A" uniqKey="Mehta A">A Mehta</name>
</author>
<author><name sortKey="Rosato, Yb" uniqKey="Rosato Y">YB Rosato</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Riordan, Ke" uniqKey="Riordan K">KE Riordan</name>
</author>
<author><name sortKey="Sorg, Ja" uniqKey="Sorg J">JA Sorg</name>
</author>
<author><name sortKey="Berube, Bj" uniqKey="Berube B">BJ Berube</name>
</author>
<author><name sortKey="Schneewind, O" uniqKey="Schneewind O">O Schneewind</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Macnab, Rm" uniqKey="Macnab R">RM Macnab</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Minamino, T" uniqKey="Minamino T">T Minamino</name>
</author>
<author><name sortKey="Gonzalez Pedrajo, B" uniqKey="Gonzalez Pedrajo B">B Gonzalez-Pedrajo</name>
</author>
<author><name sortKey="Yamaguchi, K" uniqKey="Yamaguchi K">K Yamaguchi</name>
</author>
<author><name sortKey="Aizawa, Si" uniqKey="Aizawa S">SI Aizawa</name>
</author>
<author><name sortKey="Macnab, Rm" uniqKey="Macnab R">RM Macnab</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Williams, Aw" uniqKey="Williams A">AW Williams</name>
</author>
<author><name sortKey="Yamaguchi, S" uniqKey="Yamaguchi S">S Yamaguchi</name>
</author>
<author><name sortKey="Togashi, F" uniqKey="Togashi F">F Togashi</name>
</author>
<author><name sortKey="Aizawa, Si" uniqKey="Aizawa S">SI Aizawa</name>
</author>
<author><name sortKey="Kawagishi, I" uniqKey="Kawagishi I">I Kawagishi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kutsukake, K" uniqKey="Kutsukake K">K Kutsukake</name>
</author>
<author><name sortKey="Minamino, T" uniqKey="Minamino T">T Minamino</name>
</author>
<author><name sortKey="Yokoseki, T" uniqKey="Yokoseki T">T Yokoseki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Suzuki, T" uniqKey="Suzuki T">T Suzuki</name>
</author>
<author><name sortKey="Iino, T" uniqKey="Iino T">T Iino</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Macnab, Rm" uniqKey="Macnab R">RM Macnab</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Edqvist, Pj" uniqKey="Edqvist P">PJ Edqvist</name>
</author>
<author><name sortKey="Olsson, J" uniqKey="Olsson J">J Olsson</name>
</author>
<author><name sortKey="Lavander, M" uniqKey="Lavander M">M Lavander</name>
</author>
<author><name sortKey="Sundberg, L" uniqKey="Sundberg L">L Sundberg</name>
</author>
<author><name sortKey="Forsberg, A" uniqKey="Forsberg A">A Forsberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Journet, L" uniqKey="Journet L">L Journet</name>
</author>
<author><name sortKey="Agrain, C" uniqKey="Agrain C">C Agrain</name>
</author>
<author><name sortKey="Broz, P" uniqKey="Broz P">P Broz</name>
</author>
<author><name sortKey="Cornelis, Gr" uniqKey="Cornelis G">GR Cornelis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kubori, T" uniqKey="Kubori T">T Kubori</name>
</author>
<author><name sortKey="Sukhan, A" uniqKey="Sukhan A">A Sukhan</name>
</author>
<author><name sortKey="Aizawa, Si" uniqKey="Aizawa S">SI Aizawa</name>
</author>
<author><name sortKey="Galan, Je" uniqKey="Galan J">JE Galan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tamano, K" uniqKey="Tamano K">K Tamano</name>
</author>
<author><name sortKey="Katayama, E" uniqKey="Katayama E">E Katayama</name>
</author>
<author><name sortKey="Toyotome, T" uniqKey="Toyotome T">T Toyotome</name>
</author>
<author><name sortKey="Sasakawa, C" uniqKey="Sasakawa C">C Sasakawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Collazo, Cm" uniqKey="Collazo C">CM Collazo</name>
</author>
<author><name sortKey="Zierler, Mk" uniqKey="Zierler M">MK Zierler</name>
</author>
<author><name sortKey="Galan, Je" uniqKey="Galan J">JE Galan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Payne, Pl" uniqKey="Payne P">PL Payne</name>
</author>
<author><name sortKey="Straley, Sc" uniqKey="Straley S">SC Straley</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Riordan, Ke" uniqKey="Riordan K">KE Riordan</name>
</author>
<author><name sortKey="Schneewind, O" uniqKey="Schneewind O">O Schneewind</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Agrain, C" uniqKey="Agrain C">C Agrain</name>
</author>
<author><name sortKey="Callebaut, I" uniqKey="Callebaut I">I Callebaut</name>
</author>
<author><name sortKey="Journet, L" uniqKey="Journet L">L Journet</name>
</author>
<author><name sortKey="Sorg, I" uniqKey="Sorg I">I Sorg</name>
</author>
<author><name sortKey="Paroz, C" uniqKey="Paroz C">C Paroz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Buttner, D" uniqKey="Buttner D">D Buttner</name>
</author>
<author><name sortKey="Lorenz, C" uniqKey="Lorenz C">C Lorenz</name>
</author>
<author><name sortKey="Weber, E" uniqKey="Weber E">E Weber</name>
</author>
<author><name sortKey="Bonas, U" uniqKey="Bonas U">U Bonas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sambrook, J" uniqKey="Sambrook J">J Sambrook</name>
</author>
<author><name sortKey="Russel, Dw" uniqKey="Russel D">DW Russel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Studier, Fw" uniqKey="Studier F">FW Studier</name>
</author>
<author><name sortKey="Rosenberg, Ah" uniqKey="Rosenberg A">AH Rosenberg</name>
</author>
<author><name sortKey="Dunn, Jj" uniqKey="Dunn J">JJ Dunn</name>
</author>
<author><name sortKey="Dubendorff, Jw" uniqKey="Dubendorff J">JW Dubendorff</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Studier, Fw" uniqKey="Studier F">FW Studier</name>
</author>
<author><name sortKey="Moffatt, Ba" uniqKey="Moffatt B">BA Moffatt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kleber Janke, T" uniqKey="Kleber Janke T">T Kleber-Janke</name>
</author>
<author><name sortKey="Becker, Wm" uniqKey="Becker W">WM Becker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Paulucci, Aa" uniqKey="Paulucci A">AA Paulucci</name>
</author>
<author><name sortKey="Hicks, L" uniqKey="Hicks L">L Hicks</name>
</author>
<author><name sortKey="Machado, A" uniqKey="Machado A">A Machado</name>
</author>
<author><name sortKey="Miranda, Mt" uniqKey="Miranda M">MT Miranda</name>
</author>
<author><name sortKey="Kay, Cm" uniqKey="Kay C">CM Kay</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guzzo, Cr" uniqKey="Guzzo C">CR Guzzo</name>
</author>
<author><name sortKey="Salinas, Rk" uniqKey="Salinas R">RK Salinas</name>
</author>
<author><name sortKey="Andrade, Mo" uniqKey="Andrade M">MO Andrade</name>
</author>
<author><name sortKey="Farah, Cs" uniqKey="Farah C">CS Farah</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kovach, Me" uniqKey="Kovach M">ME Kovach</name>
</author>
<author><name sortKey="Elzer, Ph" uniqKey="Elzer P">PH Elzer</name>
</author>
<author><name sortKey="Hill, Ds" uniqKey="Hill D">DS Hill</name>
</author>
<author><name sortKey="Robertson, Gt" uniqKey="Robertson G">GT Robertson</name>
</author>
<author><name sortKey="Farris, Ma" uniqKey="Farris M">MA Farris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Feyter, R" uniqKey="De Feyter R">R De Feyter</name>
</author>
<author><name sortKey="Yang, Y" uniqKey="Yang Y">Y Yang</name>
</author>
<author><name sortKey="Gabriel, Dw" uniqKey="Gabriel D">DW Gabriel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Finn, Rd" uniqKey="Finn R">RD Finn</name>
</author>
<author><name sortKey="Mistry, J" uniqKey="Mistry J">J Mistry</name>
</author>
<author><name sortKey="Tate, J" uniqKey="Tate J">J Tate</name>
</author>
<author><name sortKey="Coggill, P" uniqKey="Coggill P">P Coggill</name>
</author>
<author><name sortKey="Heger, A" uniqKey="Heger A">A Heger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crooks, Ge" uniqKey="Crooks G">GE Crooks</name>
</author>
<author><name sortKey="Hon, G" uniqKey="Hon G">G Hon</name>
</author>
<author><name sortKey="Chandonia, Jm" uniqKey="Chandonia J">JM Chandonia</name>
</author>
<author><name sortKey="Brenner, Se" uniqKey="Brenner S">SE Brenner</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id>
<journal-id journal-id-type="publisher-id">plos</journal-id>
<journal-id journal-id-type="pmc">plosone</journal-id>
<journal-title-group><journal-title>PLoS ONE</journal-title>
</journal-title-group>
<issn pub-type="epub">1932-6203</issn>
<publisher><publisher-name>Public Library of Science</publisher-name>
<publisher-loc>San Francisco, USA</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21408079</article-id>
<article-id pub-id-type="pmc">3052322</article-id>
<article-id pub-id-type="publisher-id">PONE-D-10-05447</article-id>
<article-id pub-id-type="doi">10.1371/journal.pone.0017614</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
<subj-group subj-group-type="Discipline-v2"><subject>Biology</subject>
<subj-group><subject>Biochemistry</subject>
<subj-group><subject>Proteins</subject>
<subj-group><subject>Protein Interactions</subject>
<subject>Recombinant Proteins</subject>
</subj-group>
</subj-group>
</subj-group>
<subj-group><subject>Microbiology</subject>
<subj-group><subject>Bacteriology</subject>
<subject>Host-Pathogen Interaction</subject>
<subject>Pathogenesis</subject>
</subj-group>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Structure-Function Analysis of the HrpB2-HrcU Interaction in the <italic>Xanthomonas citri</italic>
Type III Secretion System</article-title>
<alt-title alt-title-type="running-head">HrpB2 and HrcU Structure - Function Analysis</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Cappelletti</surname>
<given-names>Paola A.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>dos Santos</surname>
<given-names>Rafael Freitas</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>do Amaral</surname>
<given-names>Alexandre M.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Homem</surname>
<given-names>Rafael Augusto</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>dos Santos Souza</surname>
<given-names>Thaís</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Machado</surname>
<given-names>Marcos A.</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Farah</surname>
<given-names>Chuck S.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label>
<addr-line>Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo, Brazil</addr-line>
</aff>
<aff id="aff2"><label>2</label>
<addr-line>EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, Distrito Federal, Brazil</addr-line>
</aff>
<aff id="aff3"><label>3</label>
<addr-line>Centro APTA Citros “Sylvio Moreira”/IAC, Cordeiropolis, São Paulo, Brazil</addr-line>
</aff>
<contrib-group><contrib contrib-type="editor"><name><surname>Ho</surname>
<given-names>Paulo</given-names>
</name>
<role>Editor</role>
<xref ref-type="aff" rid="edit1"></xref>
</contrib>
</contrib-group>
<aff id="edit1">Instituto Butantan, Brazil</aff>
<author-notes><corresp id="cor1">* E-mail: <email>chsfarah@iq.usp.br</email>
</corresp>
<fn fn-type="con"><p>Conceived and designed the experiments: PAC AMA CSF. Performed the experiments: PAC RFS AMA RAH TSS. Analyzed the data: PAC AMA MAM CSF. Contributed reagents/materials/analysis tools: MAM CSF. Wrote the paper: PAC CSF.</p>
</fn>
</author-notes>
<pub-date pub-type="collection"><year>2011</year>
</pub-date>
<pub-date pub-type="epub"><day>9</day>
<month>3</month>
<year>2011</year>
</pub-date>
<volume>6</volume>
<issue>3</issue>
<elocation-id>e17614</elocation-id>
<history><date date-type="received"><day>23</day>
<month>11</month>
<year>2010</year>
</date>
<date date-type="accepted"><day>1</day>
<month>2</month>
<year>2011</year>
</date>
</history>
<permissions><copyright-statement>Cappelletti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</copyright-statement>
</permissions>
<abstract><p>Bacterial type III secretion systems deliver protein virulence factors to host cells. Here we characterize the interaction between HrpB2, a small protein secreted by the <italic>Xanthomonas citri</italic>
subsp. citri type III secretion system, and the cytosolic domain of the inner membrane protein HrcU, a paralog of the flagellar protein FlhB. We show that a recombinant fragment corresponding to the C-terminal cytosolic domain of HrcU produced in <italic>E. coli</italic>
suffers cleavage within a conserved Asn264-Pro265-Thr266-His267 (NPTH) sequence. A recombinant HrcU cytosolic domain with N264A, P265A, T266A mutations at the cleavage site (HrcU<sub>AAAH</sub>
) was not cleaved and interacted with HrpB2. Furthermore, a polypeptide corresponding to the sequence following the NPTH cleavage site also interacted with HrpB2 indicating that the site for interaction is located after the NPTH site. Non-polar deletion mutants of the <italic>hrcU</italic>
and <italic>hrpB2</italic>
genes resulted in a total loss of pathogenicity in susceptible citrus plants and disease symptoms could be recovered by expression of HrpB2 and HrcU from extrachromossomal plasmids. Complementation of the <italic>ΔhrcU</italic>
mutant with HrcU<sub>AAAH</sub>
produced canker lesions similar to those observed when complemented with wild-type HrcU. HrpB2 secretion however, was significantly reduced in the <italic>ΔhrcU</italic>
mutant complemented with HrcU<sub>AAAH,</sub>
suggesting that an intact and cleavable NPTH site in HrcU is necessary for total functionally of T3SS in <italic>X. citri</italic>
subsp. citri. Complementation of the <italic>ΔhrpB2 X. citri</italic>
subsp. citri strain with a series of <italic>hrpB2</italic>
gene mutants revealed that the highly conserved HrpB2 C-terminus is essential for T3SS-dependent development of citrus canker symptoms <italic>in planta</italic>
.</p>
</abstract>
<counts><page-count count="15"></page-count>
</counts>
</article-meta>
</front>
</pmc>
</record>
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