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Efficacy of Bacillus thuringiensis against Phyllocnistis citrella (Lepidoptera: Phyllocnistidae)

Identifieur interne : 000413 ( PascalFrancis/Curation ); précédent : 000412; suivant : 000414

Efficacy of Bacillus thuringiensis against Phyllocnistis citrella (Lepidoptera: Phyllocnistidae)

Auteurs : Carla Dias [Portugal] ; Patricia Garcia [Portugal] ; Nelson Simoes [Portugal] ; Luisa Oliveira [Portugal]

Source :

RBID : Pascal:06-0065764

Descripteurs français

English descriptors

Abstract

The purpose of this work was to analyze the efficacy of Bacillus thuringiensis Berliner on the control of Phyllocnistis citrella Stainton (Lepidoptera: Phyllocnistidae) in laboratory and field trials. In the laboratory, four B. thuringiemis were used: Dipel (commercial formulation) tested at the concentrations of 25 X 106 and 25 X 108 spores/ml and the isolates K, 6, and 15 (collections of the University of Azores) at the concentration of 25 X 106 spores/ml. A surfactant solution of nonoxinol also was tested with or without the different suspensions of B. thuringtemis. Leaves of Citrus sinensis (L.) Osbeck with second or third instars ofleafminer larvae were used in all tests. Bacterial suspensions were applied topically on the surface of intact leaf mines or by injection inside the mine, near the head of the leafminer. When injecting both concentrations of Dipel into the mines, mortality of the leafminers increased compared with the topical application, although no significant differences were observed. The addition of the nonoxinol to the Dipel suspension, applied topically, increased the effect of B. thuringiensis, but differences were not significant. The mortality of the leafminers treated only with the nonoxinol solution increased significantly 48 h after treatment, compared with the control group, suggesting an insecticidal effect of this surfactant when used at a concentration of 0.01%. All the tested B. thuringiensis were equally active against the leafminer, either when applied topically or by mine injection. Field trials showed a significant difference between larval mortality of the control group and the results observed at the trees treated with B. thuringiensis 48 h after treatments.
pA  
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A11 01  1    @1 DIAS (Carla)
A11 02  1    @1 GARCIA (Patricia)
A11 03  1    @1 SIMOES (Nelson)
A11 04  1    @1 OLIVEIRA (Luisa)
A14 01      @1 Departamento de Biologia and CIRN, Universidade dos Açores, P-9501-801 Ponta Delgada Codex @2 Azores @3 PRT @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut.
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C01 01    ENG  @0 The purpose of this work was to analyze the efficacy of Bacillus thuringiensis Berliner on the control of Phyllocnistis citrella Stainton (Lepidoptera: Phyllocnistidae) in laboratory and field trials. In the laboratory, four B. thuringiemis were used: Dipel (commercial formulation) tested at the concentrations of 25 X 106 and 25 X 108 spores/ml and the isolates K, 6, and 15 (collections of the University of Azores) at the concentration of 25 X 106 spores/ml. A surfactant solution of nonoxinol also was tested with or without the different suspensions of B. thuringtemis. Leaves of Citrus sinensis (L.) Osbeck with second or third instars ofleafminer larvae were used in all tests. Bacterial suspensions were applied topically on the surface of intact leaf mines or by injection inside the mine, near the head of the leafminer. When injecting both concentrations of Dipel into the mines, mortality of the leafminers increased compared with the topical application, although no significant differences were observed. The addition of the nonoxinol to the Dipel suspension, applied topically, increased the effect of B. thuringiensis, but differences were not significant. The mortality of the leafminers treated only with the nonoxinol solution increased significantly 48 h after treatment, compared with the control group, suggesting an insecticidal effect of this surfactant when used at a concentration of 0.01%. All the tested B. thuringiensis were equally active against the leafminer, either when applied topically or by mine injection. Field trials showed a significant difference between larval mortality of the control group and the results observed at the trees treated with B. thuringiensis 48 h after treatments.
C02 01  X    @0 002A34I03A
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C03 08  X  FRE  @0 Phyllocnistis citrella @4 INC @5 64
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C07 04  X  FRE  @0 Bactérie
C07 04  X  ENG  @0 Bacteria
C07 04  X  SPA  @0 Bacteria
C07 05  X  FRE  @0 Insecta @2 NS
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N21       @1 037
N44 01      @1 OTO
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Pascal:06-0065764

Le document en format XML

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<div type="abstract" xml:lang="en">The purpose of this work was to analyze the efficacy of Bacillus thuringiensis Berliner on the control of Phyllocnistis citrella Stainton (Lepidoptera: Phyllocnistidae) in laboratory and field trials. In the laboratory, four B. thuringiemis were used: Dipel (commercial formulation) tested at the concentrations of 25 X 10
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<sup>6</sup>
and 25 X 10
<sup>8</sup>
spores/ml and the isolates K, 6, and 15 (collections of the University of Azores) at the concentration of 25 X 10
<sup>6</sup>
spores/ml. A surfactant solution of nonoxinol also was tested with or without the different suspensions of B. thuringtemis. Leaves of Citrus sinensis (L.) Osbeck with second or third instars ofleafminer larvae were used in all tests. Bacterial suspensions were applied topically on the surface of intact leaf mines or by injection inside the mine, near the head of the leafminer. When injecting both concentrations of Dipel into the mines, mortality of the leafminers increased compared with the topical application, although no significant differences were observed. The addition of the nonoxinol to the Dipel suspension, applied topically, increased the effect of B. thuringiensis, but differences were not significant. The mortality of the leafminers treated only with the nonoxinol solution increased significantly 48 h after treatment, compared with the control group, suggesting an insecticidal effect of this surfactant when used at a concentration of 0.01%. All the tested B. thuringiensis were equally active against the leafminer, either when applied topically or by mine injection. Field trials showed a significant difference between larval mortality of the control group and the results observed at the trees treated with B. thuringiensis 48 h after treatments.</s0>
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</fC03>
<fC07 i1="01" i2="X" l="FRE">
<s0>Biopesticide</s0>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Biopesticide</s0>
</fC07>
<fC07 i1="01" i2="X" l="SPA">
<s0>Bioplaguicida</s0>
</fC07>
<fC07 i1="02" i2="X" l="FRE">
<s0>Bacillaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Bacillaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Bacillaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Bacillales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Bacillales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Bacillales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Bactérie</s0>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Bacteria</s0>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Bacteria</s0>
</fC07>
<fC07 i1="05" i2="X" l="FRE">
<s0>Insecta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG">
<s0>Insecta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA">
<s0>Insecta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE">
<s0>Arthropoda</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG">
<s0>Arthropoda</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA">
<s0>Arthropoda</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE">
<s0>Invertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG">
<s0>Invertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA">
<s0>Invertebrata</s0>
<s2>NS</s2>
</fC07>
<fN21>
<s1>037</s1>
</fN21>
<fN44 i1="01">
<s1>OTO</s1>
</fN44>
<fN82>
<s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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   |area=    OrangerV1
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   |texte=   Efficacy of Bacillus thuringiensis against Phyllocnistis citrella (Lepidoptera: Phyllocnistidae)
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