OrangerV1, PascalFrancis, Checkpoint, bibRecord, 000500

Agrobacterium-mediated transformation of citrus using two binary vectors

Identifieur interne : 000500 ( PascalFrancis/Checkpoint ); précédent : 000499; suivant : 000501

Agrobacterium-mediated transformation of citrus using two binary vectors

Auteurs : Jaya R. Soneji [États-Unis] ; CHUNXIAN CHEN [États-Unis] ; M. Nageswara Rao [États-Unis] ; SHU HUANG [États-Unis] ; Young A. Choi [États-Unis] ; Fred G. Jr Gmitter [États-Unis]

Source :

Acta horticulturae [ 0567-7572 ] ; 2007.

RBID : Pascal:07-0530119

Descripteurs français

Pascal (Inist)
- Agrume, Plante transgénique, Transfection, Transfert génétique, Transformation génétique, Vecteur, Système binaire, Agrobacterium, Citrus sinensis, Cultivar, Plasmide Ti, β-Glucuronidase, Protéine fluorescente verte, Gène hybride, Gène indicateur, Gène structure, Gène gfp, Gène gus, cv Midsweet, Poncirus.
Wicri :
- topic : Agrume, Plante transgénique.

English descriptors

KwdEn :
- Agrobacterium, Binary system, Citrus fruit, Citrus sinensis, Cultivar, Genetic transfer, Genetic transformation, Green fluorescent protein, Hybrid gene, Reporter gene, Structural gene, Ti Plasmid, Transfection, Transgenic plant, Vector, β-Glucuronidase.

Abstract

In the present study, epicotyl explants of 'Midsweet' sweet orange (Citrus sinensis L. Osb.) were transformed using a mixture of two binary vectors individually introduced into Agrobacterium strain AGL1. One binary Ti vector was assembled from pCAMBIA2301 with GUS as the reporter gene while the other binary Ti vector was assembled from pGreen0029 with GFP as the reporter gene. Two gene constructs containing specific gene fragments from Poncirus were used. The epicotyl explants were transformed by use of 1:1 mixture of AGL1 carrying the binary vectors separately. Shoots regenerated from transformed epicotyl explants on selective medium were micrografted onto 'Carrizo' seedlings. The GFP expression and the GUS activity in the leaves were also determined. Further confirmation by molecular analyses is currently underway.

Affiliations:

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Le document en format XML

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<term>Genetic transfer</term>
<term>Genetic transformation</term>
<term>Green fluorescent protein</term>
<term>Hybrid gene</term>
<term>Reporter gene</term>
<term>Structural gene</term>
<term>Ti Plasmid</term>
<term>Transfection</term>
<term>Transgenic plant</term>
<term>Vector</term>
<term>β-Glucuronidase</term>
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<term>Système binaire</term>
<term>Agrobacterium</term>
<term>Citrus sinensis</term>
<term>Cultivar</term>
<term>Plasmide Ti</term>
<term>β-Glucuronidase</term>
<term>Protéine fluorescente verte</term>
<term>Gène hybride</term>
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<term>Gène gfp</term>
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<front><div type="abstract" xml:lang="en">In the present study, epicotyl explants of 'Midsweet' sweet orange (Citrus sinensis L. Osb.) were transformed using a mixture of two binary vectors individually introduced into Agrobacterium strain AGL1. One binary Ti vector was assembled from pCAMBIA2301 with GUS as the reporter gene while the other binary Ti vector was assembled from pGreen0029 with GFP as the reporter gene. Two gene constructs containing specific gene fragments from Poncirus were used. The epicotyl explants were transformed by use of 1:1 mixture of AGL1 carrying the binary vectors separately. Shoots regenerated from transformed epicotyl explants on selective medium were micrografted onto 'Carrizo' seedlings. The GFP expression and the GUS activity in the leaves were also determined. Further confirmation by molecular analyses is currently underway.</div>
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<fA11 i1="01" i2="1"><s1>SONEJI (Jaya R.)</s1>
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<fC01 i1="01" l="ENG"><s0>In the present study, epicotyl explants of 'Midsweet' sweet orange (Citrus sinensis L. Osb.) were transformed using a mixture of two binary vectors individually introduced into Agrobacterium strain AGL1. One binary Ti vector was assembled from pCAMBIA2301 with GUS as the reporter gene while the other binary Ti vector was assembled from pGreen0029 with GFP as the reporter gene. Two gene constructs containing specific gene fragments from Poncirus were used. The epicotyl explants were transformed by use of 1:1 mixture of AGL1 carrying the binary vectors separately. Shoots regenerated from transformed epicotyl explants on selective medium were micrografted onto 'Carrizo' seedlings. The GFP expression and the GUS activity in the leaves were also determined. Further confirmation by molecular analyses is currently underway.</s0>
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<fC03 i1="11" i2="X" l="SPA"><s0>Plasmido Ti</s0>
<s5>14</s5>
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<s6>«β»-Glucuronidase</s6>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>β-Glucuronidase</s0>
<s2>FE</s2>
<s5>15</s5>
<s6>«β»-Glucuronidase</s6>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Protéine fluorescente verte</s0>
<s5>16</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Green fluorescent protein</s0>
<s5>16</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Proteína fluorescente verde</s0>
<s5>16</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Gène hybride</s0>
<s5>17</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Hybrid gene</s0>
<s5>17</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Gen híbrido</s0>
<s5>17</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Gène indicateur</s0>
<s5>18</s5>
</fC03>
<fC03 i1="15" i2="X" l="ENG"><s0>Reporter gene</s0>
<s5>18</s5>
</fC03>
<fC03 i1="15" i2="X" l="SPA"><s0>Gen marcador</s0>
<s5>18</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Gène structure</s0>
<s5>19</s5>
</fC03>
<fC03 i1="16" i2="X" l="ENG"><s0>Structural gene</s0>
<s5>19</s5>
</fC03>
<fC03 i1="16" i2="X" l="SPA"><s0>Gen estructura</s0>
<s5>19</s5>
</fC03>
<fC03 i1="17" i2="X" l="FRE"><s0>Gène gfp</s0>
<s4>INC</s4>
<s5>68</s5>
</fC03>
<fC03 i1="18" i2="X" l="FRE"><s0>Gène gus</s0>
<s4>INC</s4>
<s5>69</s5>
</fC03>
<fC03 i1="19" i2="X" l="FRE"><s0>cv Midsweet</s0>
<s4>INC</s4>
<s5>70</s5>
</fC03>
<fC03 i1="20" i2="X" l="FRE"><s0>Poncirus</s0>
<s4>INC</s4>
<s5>71</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rhizobiaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rhizobiaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rhizobiaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Bactérie</s0>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Bacteria</s0>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Bacteria</s0>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>O-Glycosidases</s0>
<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>O-Glycosidases</s0>
<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>O-Glycosidases</s0>
<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Arbre fruitier</s0>
<s5>31</s5>
</fC07>
<fC07 i1="11" i2="X" l="ENG"><s0>Fruit tree</s0>
<s5>31</s5>
</fC07>
<fC07 i1="11" i2="X" l="SPA"><s0>Arbol frutal</s0>
<s5>31</s5>
</fC07>
<fC07 i1="12" i2="X" l="FRE"><s0>Plante fruitière</s0>
<s5>32</s5>
</fC07>
<fC07 i1="12" i2="X" l="ENG"><s0>Fruit crop</s0>
<s5>32</s5>
</fC07>
<fC07 i1="12" i2="X" l="SPA"><s0>Planta frutal</s0>
<s5>32</s5>
</fC07>
<fC07 i1="13" i2="X" l="FRE"><s0>Organisme génétiquement modifié</s0>
<s5>33</s5>
</fC07>
<fC07 i1="13" i2="X" l="ENG"><s0>Genetically modified organism</s0>
<s5>33</s5>
</fC07>
<fC07 i1="13" i2="X" l="SPA"><s0>Organismo modificado genéticamente</s0>
<s5>33</s5>
</fC07>
<fC07 i1="14" i2="X" l="FRE"><s0>Plante ligneuse</s0>
<s5>34</s5>
</fC07>
<fC07 i1="14" i2="X" l="ENG"><s0>Woody plant</s0>
<s5>34</s5>
</fC07>
<fC07 i1="14" i2="X" l="SPA"><s0>Planta leñosa</s0>
<s5>34</s5>
</fC07>
<fC07 i1="15" i2="X" l="FRE"><s0>Composé biochimique</s0>
<s5>35</s5>
</fC07>
<fC07 i1="15" i2="X" l="ENG"><s0>Biochemical compound</s0>
<s5>35</s5>
</fC07>
<fC07 i1="15" i2="X" l="SPA"><s0>Compuesto bioquímico</s0>
<s5>35</s5>
</fC07>
<fC07 i1="16" i2="X" l="FRE"><s0>Biotechnologie</s0>
<s5>36</s5>
</fC07>
<fC07 i1="16" i2="X" l="ENG"><s0>Biotechnology</s0>
<s5>36</s5>
</fC07>
<fC07 i1="16" i2="X" l="SPA"><s0>Biotecnología</s0>
<s5>36</s5>
</fC07>
<fC07 i1="17" i2="X" l="FRE"><s0>Génie génétique</s0>
<s5>37</s5>
</fC07>
<fC07 i1="17" i2="X" l="ENG"><s0>Genetic engineering</s0>
<s5>37</s5>
</fC07>
<fC07 i1="17" i2="X" l="SPA"><s0>Ingeniería genética</s0>
<s5>37</s5>
</fC07>
<fC07 i1="18" i2="X" l="FRE"><s0>Arboriculture</s0>
<s5>64</s5>
</fC07>
<fC07 i1="18" i2="X" l="ENG"><s0>Arboriculture</s0>
<s5>64</s5>
</fC07>
<fC07 i1="18" i2="X" l="SPA"><s0>Arboricultura</s0>
<s5>64</s5>
</fC07>
<fC07 i1="19" i2="X" l="FRE"><s0>Horticulture</s0>
<s5>65</s5>
</fC07>
<fC07 i1="19" i2="X" l="ENG"><s0>Horticulture</s0>
<s5>65</s5>
</fC07>
<fC07 i1="19" i2="X" l="SPA"><s0>Horticultura</s0>
<s5>65</s5>
</fC07>
<fC07 i1="20" i2="X" l="FRE"><s0>Industrie fruits</s0>
<s5>66</s5>
</fC07>
<fC07 i1="20" i2="X" l="ENG"><s0>Fruit industry</s0>
<s5>66</s5>
</fC07>
<fC07 i1="20" i2="X" l="SPA"><s0>Industria frutas</s0>
<s5>66</s5>
</fC07>
<fC07 i1="21" i2="X" l="FRE"><s0>Bactérie Gram négatif</s0>
<s4>INC</s4>
<s5>84</s5>
</fC07>
<fC07 i1="22" i2="X" l="FRE"><s0>Procaryote</s0>
<s4>INC</s4>
<s5>85</s5>
</fC07>
<fN21><s1>344</s1>
</fN21>
</pA>
<pR><fA30 i1="01" i2="1" l="ENG"><s1>International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species</s1>
<s3>Daytona Beach FL USA</s3>
<s4>2005</s4>
</fA30>
</pR>
</standard>
</inist>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Floride</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Floride"><name sortKey="Soneji, Jaya R" sort="Soneji, Jaya R" uniqKey="Soneji J" first="Jaya R." last="Soneji">Jaya R. Soneji</name>
</region>
<name sortKey="Choi, Young A" sort="Choi, Young A" uniqKey="Choi Y" first="Young A." last="Choi">Young A. Choi</name>
<name sortKey="Chunxian Chen" sort="Chunxian Chen" uniqKey="Chunxian Chen" last="Chunxian Chen">CHUNXIAN CHEN</name>
<name sortKey="Gmitter, Fred G Jr" sort="Gmitter, Fred G Jr" uniqKey="Gmitter F" first="Fred G. Jr" last="Gmitter">Fred G. Jr Gmitter</name>
<name sortKey="Nageswara Rao, M" sort="Nageswara Rao, M" uniqKey="Nageswara Rao M" first="M." last="Nageswara Rao">M. Nageswara Rao</name>
<name sortKey="Shu Huang" sort="Shu Huang" uniqKey="Shu Huang" last="Shu Huang">SHU HUANG</name>
</country>
</tree>
</affiliations>
</record>

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Agrobacterium-mediated transformation of citrus using two binary vectors

Agrobacterium-mediated transformation of citrus using two binary vectors

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