Molecular Cloning of HbPR-1 Gene from Rubber Tree, Expression of HbPR-1 Gene in Nicotiana benthamiana and Its Inhibition of Phytophthora palmivora
Identifieur interne : 002080 ( Ncbi/Curation ); précédent : 002079; suivant : 002081Molecular Cloning of HbPR-1 Gene from Rubber Tree, Expression of HbPR-1 Gene in Nicotiana benthamiana and Its Inhibition of Phytophthora palmivora
Auteurs : Uraiwan Khunjan [Thaïlande, États-Unis] ; Kitiya Ekchaweng [Thaïlande, États-Unis] ; Tanate Panrat [Thaïlande] ; Miaoying Tian [États-Unis] ; Nunta Churngchow [Thaïlande]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
This is the first report to present a full-length cDNA (designated
Url:
DOI: 10.1371/journal.pone.0157591
PubMed: 27337148
PubMed Central: 4940168
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular Cloning of <italic>HbPR-1</italic> Gene from Rubber Tree, Expression of <italic>HbPR-1</italic> Gene in <italic>Nicotiana benthamiana</italic> and Its Inhibition of <italic>Phytophthora palmivora</italic></title><author><name sortKey="Khunjan, Uraiwan" sort="Khunjan, Uraiwan" uniqKey="Khunjan U" first="Uraiwan" last="Khunjan">Uraiwan Khunjan</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Ekchaweng, Kitiya" sort="Ekchaweng, Kitiya" uniqKey="Ekchaweng K" first="Kitiya" last="Ekchaweng">Kitiya Ekchaweng</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Panrat, Tanate" sort="Panrat, Tanate" uniqKey="Panrat T" first="Tanate" last="Panrat">Tanate Panrat</name><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>Digital Media Program, Prince of Songkla University International College, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Digital Media Program, Prince of Songkla University International College, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Miaoying" sort="Tian, Miaoying" uniqKey="Tian M" first="Miaoying" last="Tian">Miaoying Tian</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Churngchow, Nunta" sort="Churngchow, Nunta" uniqKey="Churngchow N" first="Nunta" last="Churngchow">Nunta Churngchow</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27337148</idno><idno type="pmc">4940168</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940168</idno><idno type="RBID">PMC:4940168</idno><idno type="doi">10.1371/journal.pone.0157591</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000395</idno><idno type="wicri:Area/Pmc/Curation">000394</idno><idno type="wicri:Area/Pmc/Checkpoint">000105</idno><idno type="wicri:Area/Ncbi/Merge">002080</idno><idno type="wicri:Area/Ncbi/Curation">002080</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular Cloning of <italic>HbPR-1</italic> Gene from Rubber Tree, Expression of <italic>HbPR-1</italic> Gene in <italic>Nicotiana benthamiana</italic> and Its Inhibition of <italic>Phytophthora palmivora</italic></title><author><name sortKey="Khunjan, Uraiwan" sort="Khunjan, Uraiwan" uniqKey="Khunjan U" first="Uraiwan" last="Khunjan">Uraiwan Khunjan</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Ekchaweng, Kitiya" sort="Ekchaweng, Kitiya" uniqKey="Ekchaweng K" first="Kitiya" last="Ekchaweng">Kitiya Ekchaweng</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Panrat, Tanate" sort="Panrat, Tanate" uniqKey="Panrat T" first="Tanate" last="Panrat">Tanate Panrat</name><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>Digital Media Program, Prince of Songkla University International College, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Digital Media Program, Prince of Songkla University International College, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Miaoying" sort="Tian, Miaoying" uniqKey="Tian M" first="Miaoying" last="Tian">Miaoying Tian</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Churngchow, Nunta" sort="Churngchow, Nunta" uniqKey="Churngchow N" first="Nunta" last="Churngchow">Nunta Churngchow</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>This is the first report to present a full-length cDNA (designated <italic>HbPR-1</italic>) encoding a putative basic HbPR-1 protein from rubber tree (<italic>Hevea brasiliensis</italic>) treated with salicylic acid. It was characterized and also expressed in <italic>Nicotiana benthamiana</italic> using <italic>Agrobacterium</italic>-mediated transient gene expression system in order to investigate the role of <italic>HbPR-1</italic> gene in rubber tree against its oomycete pathogen <italic>Phytopthora palmivora</italic> and to produce recombinant HbPR-1 protein for microbial inhibition test. The <italic>HbPR-1</italic> cDNA was 647 bp long and contained an open reading frame of 492 nucleotides encoding 163 amino acid residues with a predicted molecular mass of 17,681 Da and an isoelectric point (pI) of 8.56, demonstrating that HbPR-1 protein belongs to the basic PR-1 type. The predicted 3D structure of HbPR-1 was composed of four α-helices, three β-sheets, seven strands, and one junction loop. Expression and purification of recombinant HbPR-1 protein were successful using <italic>Agrobacterium</italic>-mediated transient expression and one-step of affinity chromatography. Heterologous expression of <italic>HbPR-1</italic> in <italic>N</italic>. <italic>benthamiana</italic> reduced necrosis areas which were inoculated with <italic>P</italic>. <italic>palmivora</italic> zoospores, indicating that the expressed HbPR-1 protein played an important role in plant resistance to pathogens. The purified recombinant HbPR-1 protein was found to inhibit 64% of <italic>P</italic>. <italic>palmivora</italic> zoospore germination on a water agar plate compared with control, suggesting that it was an antimicrobial protein against <italic>P</italic>. <italic>palmivora</italic>.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Sarowar, S" uniqKey="Sarowar S">S Sarowar</name></author><author><name sortKey="Kim, Yj" uniqKey="Kim Y">YJ Kim</name></author><author><name sortKey="Kim, En" uniqKey="Kim E">EN Kim</name></author><author><name sortKey="Kim, Kd" uniqKey="Kim K">KD Kim</name></author><author><name sortKey="Hwang, Bk" uniqKey="Hwang B">BK Hwang</name></author><author><name sortKey="Islam, R" uniqKey="Islam R">R Islam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, Q" uniqKey="Liu Q">Q Liu</name></author><author><name sortKey="Xue, Q" uniqKey="Xue Q">Q Xue</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sels, J" uniqKey="Sels J">J Sels</name></author><author><name sortKey="Mathys, J" uniqKey="Mathys J">J Mathys</name></author><author><name sortKey="De Coninck, Bma" uniqKey="De Coninck B">BMA De Coninck</name></author><author><name sortKey="Cammue, Bpa" uniqKey="Cammue B">BPA Cammue</name></author><author><name sortKey="De Belle, Mfc" uniqKey="De Belle M">MFC De Belle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, Yj" uniqKey="Kim Y">YJ Kim</name></author><author><name sortKey="Hwang, Bk" uniqKey="Hwang B">BK Hwang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Rh" uniqKey="Yang R">RH Yang</name></author><author><name sortKey="Tang, K" uniqKey="Tang K">K Tang</name></author><author><name sortKey="Liu, Ht" uniqKey="Liu H">HT Liu</name></author><author><name sortKey="Huang, Wd" uniqKey="Huang W">WD Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, F" uniqKey="Zhu F">F Zhu</name></author><author><name sortKey="Xu, M" uniqKey="Xu M">M Xu</name></author><author><name sortKey="Wang, S" uniqKey="Wang S">S Wang</name></author><author><name sortKey="Jia, S" uniqKey="Jia S">S Jia</name></author><author><name sortKey="Zhang, P" uniqKey="Zhang P">P Zhang</name></author><author><name sortKey="Lin, H" uniqKey="Lin H">H Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Loon, Lc" uniqKey="Van Loon L">LC Van Loon</name></author><author><name sortKey="Van Strien, Ea" uniqKey="Van Strien E">EA Van Strien</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, S" uniqKey="Lu S">S Lu</name></author><author><name sortKey="Friesen, Tl" uniqKey="Friesen T">TL Friesen</name></author><author><name sortKey="Faris, Jd" uniqKey="Faris J">JD Faris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, S" uniqKey="Lu S">S Lu</name></author><author><name sortKey="Faris, Jd" uniqKey="Faris J">JD Faris</name></author><author><name sortKey="Sherwood, R" uniqKey="Sherwood R">R Sherwood</name></author><author><name sortKey="Edwards, Mc" uniqKey="Edwards M">MC Edwards</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Loon, Lc" uniqKey="Van Loon L">LC Van Loon</name></author><author><name sortKey="Rep, M" uniqKey="Rep M">M Rep</name></author><author><name sortKey="Pieterse, Cmj" uniqKey="Pieterse C">CMJ Pieterse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Agrawal, Gk" uniqKey="Agrawal G">GK Agrawal</name></author><author><name sortKey="Rakwal, R" uniqKey="Rakwal R">R Rakwal</name></author><author><name sortKey="Jwa, Ns" uniqKey="Jwa N">NS Jwa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kumar, D" uniqKey="Kumar D">D Kumar</name></author><author><name sortKey="Klessig, Df" uniqKey="Klessig D">DF Klessig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mitsuhara, I" uniqKey="Mitsuhara I">I Mitsuhara</name></author><author><name sortKey="Iwai, T" uniqKey="Iwai T">T Iwai</name></author><author><name sortKey="Seo, S" uniqKey="Seo S">S Seo</name></author><author><name sortKey="Yanagawa, Y" uniqKey="Yanagawa Y">Y Yanagawa</name></author><author><name sortKey="Kawahigasi, H" uniqKey="Kawahigasi H">H Kawahigasi</name></author><author><name sortKey="Hirose, S" uniqKey="Hirose S">S Hirose</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Riviere, Mp" uniqKey="Riviere M">MP Rivière</name></author><author><name sortKey="Marais, A" uniqKey="Marais A">A Marais</name></author><author><name sortKey="Ponchet, M" uniqKey="Ponchet M">M Ponchet</name></author><author><name sortKey="Willats, W" uniqKey="Willats W">W Willats</name></author><author><name sortKey="Galiana, E" uniqKey="Galiana E">E Galiana</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shin, Sh" uniqKey="Shin S">SH Shin</name></author><author><name sortKey="Park, Jh" uniqKey="Park J">JH Park</name></author><author><name sortKey="Kim, Mj" uniqKey="Kim M">MJ Kim</name></author><author><name sortKey="Kim, Hj" uniqKey="Kim H">HJ Kim</name></author><author><name sortKey="Oh, Js" uniqKey="Oh J">JS Oh</name></author><author><name sortKey="Choi, Hk" uniqKey="Choi H">HK Choi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cutt, Jr" uniqKey="Cutt J">JR Cutt</name></author><author><name sortKey="Harpster, Mh" uniqKey="Harpster M">MH Harpster</name></author><author><name sortKey="Dixon, Dc" uniqKey="Dixon D">DC Dixon</name></author><author><name sortKey="Carr, Jp" uniqKey="Carr J">JP Carr</name></author><author><name sortKey="Dunsmuir, P" uniqKey="Dunsmuir P">P Dunsmuir</name></author><author><name sortKey="Klessig, Df" uniqKey="Klessig D">DF Klessig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Santen, K" uniqKey="Santen K">K Santén</name></author><author><name sortKey="Marttila, S" uniqKey="Marttila S">S Marttila</name></author><author><name sortKey="Liljeroth, E" uniqKey="Liljeroth E">E Liljeroth</name></author><author><name sortKey="Bryngelsson, T" uniqKey="Bryngelsson T">T Bryngelsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Breton, F" uniqKey="Breton F">F Breton</name></author><author><name sortKey="Sanier, C" uniqKey="Sanier C">C Sanier</name></author><author><name sortKey="D Uzac, J" uniqKey="D Uzac J">J D’Auzac</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wydro, M" uniqKey="Wydro M">M Wydro</name></author><author><name sortKey="Kozubek, E" uniqKey="Kozubek E">E Kozubek</name></author><author><name sortKey="Lehmann, P" uniqKey="Lehmann P">P Lehmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shamloul, M" uniqKey="Shamloul M">M Shamloul</name></author><author><name sortKey="Trusa, J" uniqKey="Trusa J">J Trusa</name></author><author><name sortKey="Mett, V" uniqKey="Mett V">V Mett</name></author><author><name sortKey="Yusibov, V" uniqKey="Yusibov V">V Yusibov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kapila, J" uniqKey="Kapila J">J Kapila</name></author><author><name sortKey="De Rycke, R" uniqKey="De Rycke R">R De Rycke</name></author><author><name sortKey="Van Montagu, M" uniqKey="Van Montagu M">M Van Montagu</name></author><author><name sortKey="Angenon, G" uniqKey="Angenon G">G Angenon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Voinnet, O" uniqKey="Voinnet O">O Voinnet</name></author><author><name sortKey="Rivas, S" uniqKey="Rivas S">S Rivas</name></author><author><name sortKey="Mestre, P" uniqKey="Mestre P">P Mestre</name></author><author><name sortKey="Baulcombe, D" uniqKey="Baulcombe D">D Baulcombe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ma, P" uniqKey="Ma P">P Ma</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author><author><name sortKey="He, H" uniqKey="He H">H He</name></author><author><name sortKey="Yang, M" uniqKey="Yang M">M Yang</name></author><author><name sortKey="Li, M" uniqKey="Li M">M Li</name></author><author><name sortKey="Zhu, X" uniqKey="Zhu X">X Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, L" uniqKey="Liu L">L Liu</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author><author><name sortKey="Tang, S" uniqKey="Tang S">S Tang</name></author><author><name sortKey="Zhao, Q" uniqKey="Zhao Q">Q Zhao</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author><author><name sortKey="Zhang, H" uniqKey="Zhang H">H Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garabagi, F" uniqKey="Garabagi F">F Garabagi</name></author><author><name sortKey="Gilbert, E" uniqKey="Gilbert E">E Gilbert</name></author><author><name sortKey="Loos, A" uniqKey="Loos A">A Loos</name></author><author><name sortKey="Mclean, Md" uniqKey="Mclean M">MD McLean</name></author><author><name sortKey="Hall, Jc" uniqKey="Hall J">JC Hall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gao, Sj" uniqKey="Gao S">SJ Gao</name></author><author><name sortKey="Damaj, Mb" uniqKey="Damaj M">MB Damaj</name></author><author><name sortKey="Park, Jw" uniqKey="Park J">JW Park</name></author><author><name sortKey="Beyene, G" uniqKey="Beyene G">G Beyene</name></author><author><name sortKey="Buenrostro Nava, Mt" uniqKey="Buenrostro Nava M">MT Buenrostro-Nava</name></author><author><name sortKey="Molina, J" uniqKey="Molina J">J Molina</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rozen, S" uniqKey="Rozen S">S Rozen</name></author><author><name sortKey="Skaletsky, H" uniqKey="Skaletsky H">H Skaletsky</name></author><author><name sortKey="Krawetz, S" uniqKey="Krawetz S">S Krawetz</name></author><author><name sortKey="Misener, S" uniqKey="Misener S">S Misener</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Altschul, Sf" uniqKey="Altschul S">SF Altschul</name></author><author><name sortKey="Gish, W" uniqKey="Gish W">W Gish</name></author><author><name sortKey="Miller, W" uniqKey="Miller W">W Miller</name></author><author><name sortKey="Myers, Ew" uniqKey="Myers E">EW Myers</name></author><author><name sortKey="Lipman, Dj" uniqKey="Lipman D">DJ Lipman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Larkin, Ma" uniqKey="Larkin M">MA Larkin</name></author><author><name sortKey="Blackshields, G" uniqKey="Blackshields G">G Blackshields</name></author><author><name sortKey="Brown, Np" uniqKey="Brown N">NP Brown</name></author><author><name sortKey="Chenna, R" uniqKey="Chenna R">R Chenna</name></author><author><name sortKey="Mcgettigan, Pa" uniqKey="Mcgettigan P">PA McGettigan</name></author><author><name sortKey="Mcwilliam, H" uniqKey="Mcwilliam H">H McWilliam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, H" uniqKey="Liu H">H Liu</name></author><author><name sortKey="Han, H" uniqKey="Han H">H Han</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Wong, L" uniqKey="Wong L">L Wong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bjellqvist, B" uniqKey="Bjellqvist B">B Bjellqvist</name></author><author><name sortKey="Hughes, Gj" uniqKey="Hughes G">GJ Hughes</name></author><author><name sortKey="Pasquali, Ch" uniqKey="Pasquali C">Ch Pasquali</name></author><author><name sortKey="Paquet, N" uniqKey="Paquet N">N Paquet</name></author><author><name sortKey="Ravier, F" uniqKey="Ravier F">F Ravier</name></author><author><name sortKey="Sanchez, Jc" uniqKey="Sanchez J">JC Sanchez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bjellqvist, B" uniqKey="Bjellqvist B">B Bjellqvist</name></author><author><name sortKey="Basse, B" uniqKey="Basse B">B Basse</name></author><author><name sortKey="Olsen, E" uniqKey="Olsen E">E Olsen</name></author><author><name sortKey="Celis, Je" uniqKey="Celis J">JE Celis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gasteiger, E" uniqKey="Gasteiger E">E Gasteiger</name></author><author><name sortKey="Hoogland, C" uniqKey="Hoogland C">C Hoogland</name></author><author><name sortKey="Gattiker, A" uniqKey="Gattiker A">A Gattiker</name></author><author><name sortKey="Duvaud, S" uniqKey="Duvaud S">S Duvaud</name></author><author><name sortKey="Wilkins, Mr" uniqKey="Wilkins M">MR Wilkins</name></author><author><name sortKey="Appel, Rd" uniqKey="Appel R">RD Appel</name></author><author><name sortKey="John, Mw" uniqKey="John M">MW John</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Castro, E" uniqKey="De Castro E">E De Castro</name></author><author><name sortKey="Sigrist, Cja" uniqKey="Sigrist C">CJA Sigrist</name></author><author><name sortKey="Gattiker, A" uniqKey="Gattiker A">A Gattiker</name></author><author><name sortKey="Bulliard, V" uniqKey="Bulliard V">V Bulliard</name></author><author><name sortKey="Langendijk Genevaux, Ps" uniqKey="Langendijk Genevaux P">PS Langendijk-Genevaux</name></author><author><name sortKey="Gasteiger, E" uniqKey="Gasteiger E">E Gasteiger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petersen, Tn" uniqKey="Petersen T">TN Petersen</name></author><author><name sortKey="Brunak, S" uniqKey="Brunak S">S Brunak</name></author><author><name sortKey="Von Heijine, G" uniqKey="Von Heijine G">G Von Heijine</name></author><author><name sortKey="Nielsen, H" uniqKey="Nielsen H">H Nielsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krogh, A" uniqKey="Krogh A">A Krogh</name></author><author><name sortKey="Larsson, B" uniqKey="Larsson B">B Larsson</name></author><author><name sortKey="Von Heijne, G" uniqKey="Von Heijne G">G Von Heijne</name></author><author><name sortKey="Sonnhammer, Ell" uniqKey="Sonnhammer E">ELL Sonnhammer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Omasits, U" uniqKey="Omasits U">U Omasits</name></author><author><name sortKey="Ahrens, Ch" uniqKey="Ahrens C">CH Ahrens</name></author><author><name sortKey="Muller, S" uniqKey="Muller S">S Müller</name></author><author><name sortKey="Wollscheid, B" uniqKey="Wollscheid B">B Wollscheid</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maheshwari, S" uniqKey="Maheshwari S">S Maheshwari</name></author><author><name sortKey="Brylinski, M" uniqKey="Brylinski M">M Brylinski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arnold, K" uniqKey="Arnold K">K Arnold</name></author><author><name sortKey="Bordoli, L" uniqKey="Bordoli L">L Bordoli</name></author><author><name sortKey="Kopp, J" uniqKey="Kopp J">J Kopp</name></author><author><name sortKey="Schwede, T" uniqKey="Schwede T">T Schwede</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bordoli, L" uniqKey="Bordoli L">L Bordoli</name></author><author><name sortKey="Kiefer, F" uniqKey="Kiefer F">F Kiefer</name></author><author><name sortKey="Arnold, K" uniqKey="Arnold K">K Arnold</name></author><author><name sortKey="Benkert, P" uniqKey="Benkert P">P Benkert</name></author><author><name sortKey="Battey, J" uniqKey="Battey J">J Battey</name></author><author><name sortKey="Schwede, T" uniqKey="Schwede T">T Schwede</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Biasini, M" uniqKey="Biasini M">M Biasini</name></author><author><name sortKey="Bienert, S" uniqKey="Bienert S">S Bienert</name></author><author><name sortKey="Waterhouse, A" uniqKey="Waterhouse A">A Waterhouse</name></author><author><name sortKey="Arnold, K" uniqKey="Arnold K">K Arnold</name></author><author><name sortKey="Studer, G" uniqKey="Studer G">G Studer</name></author><author><name sortKey="Schmidt, T" uniqKey="Schmidt T">T Schmidt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roy, A" uniqKey="Roy A">A Roy</name></author><author><name sortKey="Kucukural, A" uniqKey="Kucukural A">A Kucukural</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, J" uniqKey="Yang J">J Yang</name></author><author><name sortKey="Yan, R" uniqKey="Yan R">R Yan</name></author><author><name sortKey="Roy, A" uniqKey="Roy A">A Roy</name></author><author><name sortKey="Xu, D" uniqKey="Xu D">D Xu</name></author><author><name sortKey="Poisson, J" uniqKey="Poisson J">J Poisson</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laskowski, Ra" uniqKey="Laskowski R">RA Laskowski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Berman, Hm" uniqKey="Berman H">HM Berman</name></author><author><name sortKey="Westbrook, J" uniqKey="Westbrook J">J Westbrook</name></author><author><name sortKey="Feng, Z" uniqKey="Feng Z">Z Feng</name></author><author><name sortKey="Gilliland, G" uniqKey="Gilliland G">G Gilliland</name></author><author><name sortKey="Bhat, Tn" uniqKey="Bhat T">TN Bhat</name></author><author><name sortKey="Weissig, H" uniqKey="Weissig H">H Weissig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Delano, Wl" uniqKey="Delano W">WL DeLano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goodin, Mm" uniqKey="Goodin M">MM Goodin</name></author><author><name sortKey="Dietzgen, Rg" uniqKey="Dietzgen R">RG Dietzgen</name></author><author><name sortKey="Schichnes, D" uniqKey="Schichnes D">D Schichnes</name></author><author><name sortKey="Ruzin, S" uniqKey="Ruzin S">S Ruzin</name></author><author><name sortKey="Jackson, Ao" uniqKey="Jackson A">AO Jackson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kruger, J" uniqKey="Kruger J">J Kruger</name></author><author><name sortKey="Thomas, Cm" uniqKey="Thomas C">CM Thomas</name></author><author><name sortKey="Golstein, C" uniqKey="Golstein C">C Golstein</name></author><author><name sortKey="Dixon, Ms" uniqKey="Dixon M">MS Dixon</name></author><author><name sortKey="Smoker, M" uniqKey="Smoker M">M Smoker</name></author><author><name sortKey="Tang, S" uniqKey="Tang S">S Tang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lindbo, Ja" uniqKey="Lindbo J">JA Lindbo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ausubel, Fm" uniqKey="Ausubel F">FM Ausubel</name></author><author><name sortKey="Brent, R" uniqKey="Brent R">R Brent</name></author><author><name sortKey="Kingston, Re" uniqKey="Kingston R">RE Kingston</name></author><author><name sortKey="Moore, Dd" uniqKey="Moore D">DD Moore</name></author><author><name sortKey="Seidman, Jg" uniqKey="Seidman J">JG Seidman</name></author><author><name sortKey="Smith, Ja" uniqKey="Smith J">JA Smith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Wit, Pjgm" uniqKey="De Wit P">PJGM De Wit</name></author><author><name sortKey="Spikman, G" uniqKey="Spikman G">G Spikman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sambrook, J" uniqKey="Sambrook J">J Sambrook</name></author><author><name sortKey="Fritsch, Ef" uniqKey="Fritsch E">EF Fritsch</name></author><author><name sortKey="Maniatis, T" uniqKey="Maniatis T">T Maniatis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marchler Bauer, A" uniqKey="Marchler Bauer A">A Marchler-Bauer</name></author><author><name sortKey="Derbyshire, Mk" uniqKey="Derbyshire M">MK Derbyshire</name></author><author><name sortKey="Gonzales, Nr" uniqKey="Gonzales N">NR Gonzales</name></author><author><name sortKey="Lu, S" uniqKey="Lu S">S Lu</name></author><author><name sortKey="Chitsaz, F" uniqKey="Chitsaz F">F Chitsaz</name></author><author><name sortKey="Geer, Ly" uniqKey="Geer L">LY Geer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kitajima, S" uniqKey="Kitajima S">S Kitajima</name></author><author><name sortKey="Sato, F" uniqKey="Sato F">F Sato</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shikamoto, Y" uniqKey="Shikamoto Y">Y Shikamoto</name></author><author><name sortKey="Suto, K" uniqKey="Suto K">K Suto</name></author><author><name sortKey="Yamazaki, Y" uniqKey="Yamazaki Y">Y Yamazaki</name></author><author><name sortKey="Morita, T" uniqKey="Morita T">T Morita</name></author><author><name sortKey="Mizuno, H" uniqKey="Mizuno H">H Mizuno</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buch, F" uniqKey="Buch F">F Buch</name></author><author><name sortKey="Pauchet, Y" uniqKey="Pauchet Y">Y Pauchet</name></author><author><name sortKey="Rott, M" uniqKey="Rott M">M Rott</name></author><author><name sortKey="Mithofer, A" uniqKey="Mithofer A">A Mithöfer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Park, Sc" uniqKey="Park S">SC Park</name></author><author><name sortKey="Lee, Jr" uniqKey="Lee J">JR Lee</name></author><author><name sortKey="Kim, Jy" uniqKey="Kim J">JY Kim</name></author><author><name sortKey="Hwang, I" uniqKey="Hwang I">I Hwang</name></author><author><name sortKey="Nah, Jw" uniqKey="Nah J">JW Nah</name></author><author><name sortKey="Cheong, H" uniqKey="Cheong H">H Cheong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Yl" uniqKey="Wang Y">YL Wang</name></author><author><name sortKey="Kuo, Jh" uniqKey="Kuo J">JH Kuo</name></author><author><name sortKey="Lee, Sc" uniqKey="Lee S">SC Lee</name></author><author><name sortKey="Liu, Js" uniqKey="Liu J">JS Liu</name></author><author><name sortKey="Hsieh, Yc" uniqKey="Hsieh Y">YC Hsieh</name></author><author><name sortKey="Shih, Yt" uniqKey="Shih Y">YT Shih</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laitaoja, M" uniqKey="Laitaoja M">M Laitaoja</name></author><author><name sortKey="Valjakka J Nis, J" uniqKey="Valjakka J Nis J">J Valjakka Jänis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Zt" uniqKey="Li Z">ZT Li</name></author><author><name sortKey="Dhekney, Sa" uniqKey="Dhekney S">SA Dhekney</name></author><author><name sortKey="Gray, Dj" uniqKey="Gray D">DJ Gray</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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