Molecular Cloning of HbPR-1 Gene from Rubber Tree, Expression of HbPR-1 Gene in Nicotiana benthamiana and Its Inhibition of Phytophthora palmivora
Identifieur interne : 000105 ( Pmc/Checkpoint ); précédent : 000104; suivant : 000106Molecular Cloning of HbPR-1 Gene from Rubber Tree, Expression of HbPR-1 Gene in Nicotiana benthamiana and Its Inhibition of Phytophthora palmivora
Auteurs : Uraiwan Khunjan [Thaïlande, États-Unis] ; Kitiya Ekchaweng [Thaïlande, États-Unis] ; Tanate Panrat [Thaïlande] ; Miaoying Tian [États-Unis] ; Nunta Churngchow [Thaïlande]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
This is the first report to present a full-length cDNA (designated
Url:
DOI: 10.1371/journal.pone.0157591
PubMed: 27337148
PubMed Central: 4940168
Affiliations:
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PMC:4940168Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular Cloning of <italic>HbPR-1</italic> Gene from Rubber Tree, Expression of <italic>HbPR-1</italic> Gene in <italic>Nicotiana benthamiana</italic> and Its Inhibition of <italic>Phytophthora palmivora</italic></title><author><name sortKey="Khunjan, Uraiwan" sort="Khunjan, Uraiwan" uniqKey="Khunjan U" first="Uraiwan" last="Khunjan">Uraiwan Khunjan</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Ekchaweng, Kitiya" sort="Ekchaweng, Kitiya" uniqKey="Ekchaweng K" first="Kitiya" last="Ekchaweng">Kitiya Ekchaweng</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Panrat, Tanate" sort="Panrat, Tanate" uniqKey="Panrat T" first="Tanate" last="Panrat">Tanate Panrat</name><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>Digital Media Program, Prince of Songkla University International College, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Digital Media Program, Prince of Songkla University International College, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Miaoying" sort="Tian, Miaoying" uniqKey="Tian M" first="Miaoying" last="Tian">Miaoying Tian</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Churngchow, Nunta" sort="Churngchow, Nunta" uniqKey="Churngchow N" first="Nunta" last="Churngchow">Nunta Churngchow</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27337148</idno><idno type="pmc">4940168</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940168</idno><idno type="RBID">PMC:4940168</idno><idno type="doi">10.1371/journal.pone.0157591</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000395</idno><idno type="wicri:Area/Pmc/Curation">000394</idno><idno type="wicri:Area/Pmc/Checkpoint">000105</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular Cloning of <italic>HbPR-1</italic> Gene from Rubber Tree, Expression of <italic>HbPR-1</italic> Gene in <italic>Nicotiana benthamiana</italic> and Its Inhibition of <italic>Phytophthora palmivora</italic></title><author><name sortKey="Khunjan, Uraiwan" sort="Khunjan, Uraiwan" uniqKey="Khunjan U" first="Uraiwan" last="Khunjan">Uraiwan Khunjan</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Ekchaweng, Kitiya" sort="Ekchaweng, Kitiya" uniqKey="Ekchaweng K" first="Kitiya" last="Ekchaweng">Kitiya Ekchaweng</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Panrat, Tanate" sort="Panrat, Tanate" uniqKey="Panrat T" first="Tanate" last="Panrat">Tanate Panrat</name><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>Digital Media Program, Prince of Songkla University International College, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Digital Media Program, Prince of Songkla University International College, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Miaoying" sort="Tian, Miaoying" uniqKey="Tian M" first="Miaoying" last="Tian">Miaoying Tian</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI</wicri:regionArea><wicri:noRegion>HI</wicri:noRegion></affiliation></author><author><name sortKey="Churngchow, Nunta" sort="Churngchow, Nunta" uniqKey="Churngchow N" first="Nunta" last="Churngchow">Nunta Churngchow</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla</wicri:regionArea><wicri:noRegion>Songkhla</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>This is the first report to present a full-length cDNA (designated <italic>HbPR-1</italic>) encoding a putative basic HbPR-1 protein from rubber tree (<italic>Hevea brasiliensis</italic>) treated with salicylic acid. It was characterized and also expressed in <italic>Nicotiana benthamiana</italic> using <italic>Agrobacterium</italic>-mediated transient gene expression system in order to investigate the role of <italic>HbPR-1</italic> gene in rubber tree against its oomycete pathogen <italic>Phytopthora palmivora</italic> and to produce recombinant HbPR-1 protein for microbial inhibition test. The <italic>HbPR-1</italic> cDNA was 647 bp long and contained an open reading frame of 492 nucleotides encoding 163 amino acid residues with a predicted molecular mass of 17,681 Da and an isoelectric point (pI) of 8.56, demonstrating that HbPR-1 protein belongs to the basic PR-1 type. The predicted 3D structure of HbPR-1 was composed of four α-helices, three β-sheets, seven strands, and one junction loop. Expression and purification of recombinant HbPR-1 protein were successful using <italic>Agrobacterium</italic>-mediated transient expression and one-step of affinity chromatography. Heterologous expression of <italic>HbPR-1</italic> in <italic>N</italic>. <italic>benthamiana</italic> reduced necrosis areas which were inoculated with <italic>P</italic>. <italic>palmivora</italic> zoospores, indicating that the expressed HbPR-1 protein played an important role in plant resistance to pathogens. The purified recombinant HbPR-1 protein was found to inhibit 64% of <italic>P</italic>. <italic>palmivora</italic> zoospore germination on a water agar plate compared with control, suggesting that it was an antimicrobial protein against <italic>P</italic>. <italic>palmivora</italic>.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Sarowar, S" uniqKey="Sarowar S">S Sarowar</name></author><author><name sortKey="Kim, Yj" uniqKey="Kim Y">YJ Kim</name></author><author><name sortKey="Kim, En" uniqKey="Kim E">EN Kim</name></author><author><name sortKey="Kim, Kd" uniqKey="Kim K">KD Kim</name></author><author><name sortKey="Hwang, Bk" uniqKey="Hwang B">BK Hwang</name></author><author><name sortKey="Islam, R" uniqKey="Islam R">R Islam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, Q" uniqKey="Liu Q">Q Liu</name></author><author><name sortKey="Xue, Q" uniqKey="Xue Q">Q 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27337148</article-id><article-id pub-id-type="pmc">4940168</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0157591</article-id><article-id pub-id-type="publisher-id">PONE-D-16-09457</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteins</subject><subj-group><subject>Recombinant Proteins</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Bacteria</subject><subj-group><subject>Agrobacteria</subject><subj-group><subject>Agrobacterium Tumefaciens</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Microbiology</subject><subj-group><subject>Plant Microbiology</subject><subj-group><subject>Agrobacteria</subject><subj-group><subject>Agrobacterium Tumefaciens</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Microbiology</subject><subj-group><subject>Agrobacteria</subject><subj-group><subject>Agrobacterium Tumefaciens</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Molecular Biology</subject><subj-group><subject>Macromolecular Structure Analysis</subject><subj-group><subject>Protein Structure</subject><subj-group><subject>Protein Structure Prediction</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteins</subject><subj-group><subject>Protein Structure</subject><subj-group><subject>Protein Structure Prediction</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Anatomy</subject><subj-group><subject>Leaves</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Plant Biochemistry</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Biochemistry</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Physical Sciences</subject><subj-group><subject>Chemistry</subject><subj-group><subject>Polymer Chemistry</subject><subj-group><subject>Macromolecules</subject><subj-group><subject>Polymers</subject><subj-group><subject>Elastomers</subject><subj-group><subject>Rubber</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Physical Sciences</subject><subj-group><subject>Materials Science</subject><subj-group><subject>Materials by Structure</subject><subj-group><subject>Polymers</subject><subj-group><subject>Elastomers</subject><subj-group><subject>Rubber</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Pathology</subject><subj-group><subject>Plant Pathogens</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Agriculture</subject><subj-group><subject>Crop Science</subject><subj-group><subject>Crops</subject><subj-group><subject>Fruits</subject><subj-group><subject>Figs</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Plants</subject><subj-group><subject>Fruits</subject><subj-group><subject>Figs</subject></subj-group></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Molecular Cloning of <italic>HbPR-1</italic> Gene from Rubber Tree, Expression of <italic>HbPR-1</italic> Gene in <italic>Nicotiana benthamiana</italic> and Its Inhibition of <italic>Phytophthora palmivora</italic></article-title><alt-title alt-title-type="running-head">Expression of <italic>HbPR-1</italic> from Rubber Tree in <italic>Nicotiana benthamiana</italic></alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Khunjan</surname><given-names>Uraiwan</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ekchaweng</surname><given-names>Kitiya</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Panrat</surname><given-names>Tanate</given-names></name><xref ref-type="aff" rid="aff003"><sup>3</sup></xref><xref ref-type="aff" rid="aff004"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Tian</surname><given-names>Miaoying</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Churngchow</surname><given-names>Nunta</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Manoa, HI, United States of America</addr-line></aff><aff id="aff003"><label>3</label><addr-line>Digital Media Program, Prince of Songkla University International College, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></aff><aff id="aff004"><label>4</label><addr-line>Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Chen</surname><given-names>Chunxian</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>USDA/ARS, UNITED STATES</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: NC MT UK. Performed the experiments: UK TP KE. Analyzed the data: NC MT UK. Contributed reagents/materials/analysis tools: NC MT. Wrote the paper: UK. MT NC: Revised and proofread the paper.</p></fn><corresp id="cor001">* E-mail: <email>nunta.c@psu.ac.th</email></corresp></author-notes><pub-date pub-type="epub"><day>23</day><month>6</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>11</volume><issue>6</issue><elocation-id>e0157591</elocation-id><history><date date-type="received"><day>20</day><month>3</month><year>2016</year></date><date date-type="accepted"><day>1</day><month>6</month><year>2016</year></date></history><permissions><license xlink:href="https://creativecommons.org/publicdomain/zero/1.0/"><license-p>This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/publicdomain/zero/1.0/">Creative Commons CC0</ext-link> public domain dedication.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="pone.0157591.pdf"></self-uri><abstract><p>This is the first report to present a full-length cDNA (designated <italic>HbPR-1</italic>) encoding a putative basic HbPR-1 protein from rubber tree (<italic>Hevea brasiliensis</italic>) treated with salicylic acid. It was characterized and also expressed in <italic>Nicotiana benthamiana</italic> using <italic>Agrobacterium</italic>-mediated transient gene expression system in order to investigate the role of <italic>HbPR-1</italic> gene in rubber tree against its oomycete pathogen <italic>Phytopthora palmivora</italic> and to produce recombinant HbPR-1 protein for microbial inhibition test. The <italic>HbPR-1</italic> cDNA was 647 bp long and contained an open reading frame of 492 nucleotides encoding 163 amino acid residues with a predicted molecular mass of 17,681 Da and an isoelectric point (pI) of 8.56, demonstrating that HbPR-1 protein belongs to the basic PR-1 type. The predicted 3D structure of HbPR-1 was composed of four α-helices, three β-sheets, seven strands, and one junction loop. Expression and purification of recombinant HbPR-1 protein were successful using <italic>Agrobacterium</italic>-mediated transient expression and one-step of affinity chromatography. Heterologous expression of <italic>HbPR-1</italic> in <italic>N</italic>. <italic>benthamiana</italic> reduced necrosis areas which were inoculated with <italic>P</italic>. <italic>palmivora</italic> zoospores, indicating that the expressed HbPR-1 protein played an important role in plant resistance to pathogens. The purified recombinant HbPR-1 protein was found to inhibit 64% of <italic>P</italic>. <italic>palmivora</italic> zoospore germination on a water agar plate compared with control, suggesting that it was an antimicrobial protein against <italic>P</italic>. <italic>palmivora</italic>.</p></abstract><funding-group><award-group id="award001"><funding-source><institution>The Office of the Higher Education Commission Strategic Scholarships Fellowships Frontier Research Networks (Specific for Southern region)</institution></funding-source><principal-award-recipient><name><surname>Khunjan</surname><given-names>Uraiwan</given-names></name></principal-award-recipient></award-group><award-group id="award002"><funding-source><institution>The government budget of Prince of Songkla University</institution></funding-source></award-group><award-group id="award003"><funding-source><institution>The grant from the Biochemistry Excellence Strengthening Program, Faculty of Science, Prince of Songkla University</institution></funding-source></award-group><award-group id="award004"><funding-source><institution>The Research Funding of the Graduate School, Prince of Songkla University</institution></funding-source></award-group><funding-statement>This work was funded by the Office of the Higher Education Commission Strategic Scholarships Fellowships Frontier Research Networks (Specific for Southern region), the government budget of Prince of Songkla University, a grant from the Biochemistry Excellence Strengthening Program, Faculty of Science, Prince of Songkla University 4, and research funding of the Graduate School, Prince of Songkla University.</funding-statement></funding-group><counts><fig-count count="7"></fig-count><table-count count="0"></table-count><page-count count="20"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper and its Supporting Information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper and its Supporting Information files.</p></notes></front></pmc><affiliations><list><country><li>Thaïlande</li><li>États-Unis</li></country></list><tree><country name="Thaïlande"><noRegion><name sortKey="Khunjan, Uraiwan" sort="Khunjan, Uraiwan" uniqKey="Khunjan U" first="Uraiwan" last="Khunjan">Uraiwan Khunjan</name></noRegion><name sortKey="Churngchow, Nunta" sort="Churngchow, Nunta" uniqKey="Churngchow N" first="Nunta" last="Churngchow">Nunta Churngchow</name><name sortKey="Ekchaweng, Kitiya" sort="Ekchaweng, Kitiya" uniqKey="Ekchaweng K" first="Kitiya" last="Ekchaweng">Kitiya Ekchaweng</name><name sortKey="Panrat, Tanate" sort="Panrat, Tanate" uniqKey="Panrat T" first="Tanate" last="Panrat">Tanate Panrat</name><name sortKey="Panrat, Tanate" sort="Panrat, Tanate" uniqKey="Panrat T" first="Tanate" last="Panrat">Tanate Panrat</name></country><country name="États-Unis"><noRegion><name sortKey="Khunjan, Uraiwan" sort="Khunjan, Uraiwan" uniqKey="Khunjan U" first="Uraiwan" last="Khunjan">Uraiwan Khunjan</name></noRegion><name sortKey="Ekchaweng, Kitiya" sort="Ekchaweng, Kitiya" uniqKey="Ekchaweng K" first="Kitiya" last="Ekchaweng">Kitiya Ekchaweng</name><name sortKey="Tian, Miaoying" sort="Tian, Miaoying" uniqKey="Tian M" first="Miaoying" last="Tian">Miaoying Tian</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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