The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck).
Identifieur interne : 000116 ( Ncbi/Curation ); précédent : 000115; suivant : 000117The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck).
Auteurs : R L Boscariol [Brésil] ; W A B. Almeida ; M T V C. Derbyshire ; F A A. Mourão Filho ; B M J. MendesSource :
- Plant cell reports [ 0721-7714 ] ; 2003.
English descriptors
- KwdEn :
- Citrus sinensis (drug effects), Citrus sinensis (genetics), Citrus sinensis (growth & development), Culture Techniques, Fructose (pharmacology), Gene Expression Regulation, Enzymologic (drug effects), Genetic Markers (genetics), Mannose (pharmacology), Mannose-6-Phosphate Isomerase (genetics), Mannose-6-Phosphate Isomerase (metabolism), Plants, Genetically Modified (drug effects), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Sucrose (pharmacology).
- MESH :
- chemical , genetics : Genetic Markers, Mannose-6-Phosphate Isomerase.
- chemical , metabolism : Mannose-6-Phosphate Isomerase.
- chemical , pharmacology : Fructose, Mannose, Sucrose.
- drug effects : Citrus sinensis, Gene Expression Regulation, Enzymologic, Plants, Genetically Modified.
- genetics : Citrus sinensis, Plants, Genetically Modified.
- growth & development : Citrus sinensis, Plants, Genetically Modified.
- Culture Techniques.
Abstract
A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides.
DOI: 10.1007/s00299-003-0654-1
PubMed: 12879258
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Osbeck).</title><author><name sortKey="Boscariol, R L" sort="Boscariol, R L" uniqKey="Boscariol R" first="R L" last="Boscariol">R L Boscariol</name><affiliation wicri:level="4"><nlm:affiliation>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13 400-970 São Paulo, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13 400-970 São Paulo</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Almeida, W A B" sort="Almeida, W A B" uniqKey="Almeida W" first="W A B" last="Almeida">W A B. Almeida</name></author><author><name sortKey="Derbyshire, M T V C" sort="Derbyshire, M T V C" uniqKey="Derbyshire M" first="M T V C" last="Derbyshire">M T V C. Derbyshire</name></author><author><name sortKey="Mourao Filho, F A A" sort="Mourao Filho, F A A" uniqKey="Mourao Filho F" first="F A A" last="Mourão Filho">F A A. Mourão Filho</name></author><author><name sortKey="Mendes, B M J" sort="Mendes, B M J" uniqKey="Mendes B" first="B M J" last="Mendes">B M J. Mendes</name></author></analytic><series><title level="j">Plant cell reports</title><idno type="ISSN">0721-7714</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus sinensis (drug effects)</term><term>Citrus sinensis (genetics)</term><term>Citrus sinensis (growth & development)</term><term>Culture Techniques</term><term>Fructose (pharmacology)</term><term>Gene Expression Regulation, Enzymologic (drug effects)</term><term>Genetic Markers (genetics)</term><term>Mannose (pharmacology)</term><term>Mannose-6-Phosphate Isomerase (genetics)</term><term>Mannose-6-Phosphate Isomerase (metabolism)</term><term>Plants, Genetically Modified (drug effects)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Sucrose (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Genetic Markers</term><term>Mannose-6-Phosphate Isomerase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mannose-6-Phosphate Isomerase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Fructose</term><term>Mannose</term><term>Sucrose</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Citrus sinensis</term><term>Gene Expression Regulation, Enzymologic</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Citrus sinensis</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Culture Techniques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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