Characterization of Some Cylindrocladium Species with Three-Septate Conidia Using Morphology, Isozyme Banding Patterns and DNA Polymorphisms
Identifieur interne : 003B52 ( Main/Merge ); précédent : 003B51; suivant : 003B53Characterization of Some Cylindrocladium Species with Three-Septate Conidia Using Morphology, Isozyme Banding Patterns and DNA Polymorphisms
Auteurs : P. W. Crous [Afrique du Sud] ; B. J. H. Janse [Afrique du Sud] ; D. Victor [Afrique du Sud] ; G. F. Marais [Afrique du Sud] ; A. C. Alfenas [Brésil]Source :
- Systematic and Applied Microbiology [ 0723-2020 ] ; 1993.
English descriptors
Abstract
Summary: Morphological and growth characteristics, esterase isozyme banding patterns and total DNA profiles were obtained for isolates of Cylindrocladium citri Fawcett & Klotz, C. crotalariae (Loos) Bell & Sobers, C. ilicicola (Hawley) Boedijn & Reitsma, C. penicilloides (Tub.) Tubaki, C. spathulatum El-Gholl, Kimbrough, Barnard, Alfieri & Schoulties as well as seven previously unidentified isolates. The study of zymograms and total DNA banding patterns enhanced our ability to determine relationships among and within species. Each procedure allowed us to differentiate among all the species except between C. penicilloides and C. citri, which appeared distinct only on the basis of their esterase zymograms. C. penicilloides may therefore be regarded as an intraspecific variant of C. citri. Furthermore, a new species, C. variabile, and its teleomorph, Calonectria variabilis, are described. The new species has distinct vesicle morphology, esterase and total DNA banding patterns.
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DOI: 10.1016/S0723-2020(11)80479-0
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Tubaki, C. spathulatum El-Gholl, Kimbrough, Barnard, Alfieri & Schoulties as well as seven previously unidentified isolates. The study of zymograms and total DNA banding patterns enhanced our ability to determine relationships among and within species. Each procedure allowed us to differentiate among all the species except between C. penicilloides and C. citri, which appeared distinct only on the basis of their esterase zymograms. C. penicilloides may therefore be regarded as an intraspecific variant of C. citri. Furthermore, a new species, C. variabile, and its teleomorph, Calonectria variabilis, are described. The new species has distinct vesicle morphology, esterase and total DNA banding patterns.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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