Normalizing sweet orange (C. sinensis (L.) Osbeck) somatic embryogenesis with semi-permeable membranes
Identifieur interne : 002E26 ( Main/Merge ); précédent : 002E25; suivant : 002E27Normalizing sweet orange (C. sinensis (L.) Osbeck) somatic embryogenesis with semi-permeable membranes
Auteurs : Randall P. Niedz [États-Unis] ; Scott E. Hyndman [États-Unis] ; Eldridge T. Wynn [États-Unis] ; Michael G. Bausher [États-Unis]Source :
- In vitro cellular & developmental biology. Plant [ 1054-5476 ] ; 2002.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Cellulose, Saccharose.
English descriptors
- KwdEn :
Abstract
Development of citrus somatic embryos initiated from embryogenic callus generally results in abnormal morphogenesis of somatic embryos. To normalize development, glycerol-induced globular-stage somatic embryos of sweet orange [C. sinensis (L.) Osbeck cv. 'Hamlin'] were cultured on 6000-8000MW cutoff cellulose acetate, >400000MW cutoff cellulose acetate, nitrocellulose, polyvinylidene fluoride (PVDF), cellulose filter paper, or positive or neutral charged nylon membranes. Only the two cellulose acetate membranes resulted in the development of normal, two-cotyledon, bipolar, heart-shaped embryos, and no aberrant teratoma-like structures. Heart-shaped embryos developed and germinated normally on Murashige and Tucker basal medium with 0.5% sucrose and 1 μM gibberellic acid. Culture of embryogenic callus directly onto cellulose membranes also resulted in the development of normal heart-shaped embryos, indicating that glycerol induction of globular-stage embryos is not necessary. Heart-shaped embryos were not observed when the osmotic potential of the medium was increased by the addition of 2.5-15% polyethylene glycol; neither were they observed when the matric potential of the medium was increased by increasing the gelling agent concentrations of agar and Gelrite from 0.8% to 3% and 0.15% to 0.9%, respectively.
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Osbeck) somatic embryogenesis with semi-permeable membranes</title><author><name sortKey="Niedz, Randall P" sort="Niedz, Randall P" uniqKey="Niedz R" first="Randall P." last="Niedz">Randall P. Niedz</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Agricultural Research Service, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Ft.</s1><s2>Pierce, FL 34945-3030</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Hyndman, Scott E" sort="Hyndman, Scott E" uniqKey="Hyndman S" first="Scott E." last="Hyndman">Scott E. 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Bausher</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Agricultural Research Service, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Ft.</s1><s2>Pierce, FL 34945-3030</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author></analytic><series><title level="j" type="main">In vitro cellular & developmental biology. Plant</title><title level="j" type="abbreviated">In vitro cell. dev. biol., Plant</title><idno type="ISSN">1054-5476</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">In vitro cellular & developmental biology. Plant</title><title level="j" type="abbreviated">In vitro cell. dev. biol., Plant</title><idno type="ISSN">1054-5476</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetate</term><term>Cellulose</term><term>Cellulose acetate</term><term>Citrus sinensis</term><term>Culture medium</term><term>Embryonic development</term><term>Experimental protocol</term><term>Gibberellic acid</term><term>Glycerol</term><term>Morphogenesis</term><term>Organogenesis</term><term>Somatic embryo</term><term>Sucrose</term><term>Water potential</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Embryon somatique</term><term>Développement embryonnaire</term><term>Organogenèse</term><term>Morphogenèse</term><term>Protocole expérimental</term><term>Cellulose acétate</term><term>Milieu culture</term><term>Potentiel hydrique</term><term>Citrus sinensis</term><term>Glycérol</term><term>Cellulose</term><term>Acétate</term><term>Saccharose</term><term>Gibbérellique acide</term><term>Membrane semi perméable</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Cellulose</term><term>Saccharose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Development of citrus somatic embryos initiated from embryogenic callus generally results in abnormal morphogenesis of somatic embryos. To normalize development, glycerol-induced globular-stage somatic embryos of sweet orange [C. sinensis (L.) Osbeck cv. 'Hamlin'] were cultured on 6000-8000MW cutoff cellulose acetate, >400000MW cutoff cellulose acetate, nitrocellulose, polyvinylidene fluoride (PVDF), cellulose filter paper, or positive or neutral charged nylon membranes. Only the two cellulose acetate membranes resulted in the development of normal, two-cotyledon, bipolar, heart-shaped embryos, and no aberrant teratoma-like structures. Heart-shaped embryos developed and germinated normally on Murashige and Tucker basal medium with 0.5% sucrose and 1 μM gibberellic acid. Culture of embryogenic callus directly onto cellulose membranes also resulted in the development of normal heart-shaped embryos, indicating that glycerol induction of globular-stage embryos is not necessary. Heart-shaped embryos were not observed when the osmotic potential of the medium was increased by the addition of 2.5-15% polyethylene glycol; neither were they observed when the matric potential of the medium was increased by increasing the gelling agent concentrations of agar and Gelrite from 0.8% to 3% and 0.15% to 0.9%, respectively.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="États-Unis"><region name="Floride"><name sortKey="Niedz, Randall P" sort="Niedz, Randall P" uniqKey="Niedz R" first="Randall P." last="Niedz">Randall P. Niedz</name></region><name sortKey="Bausher, Michael G" sort="Bausher, Michael G" uniqKey="Bausher M" first="Michael G." last="Bausher">Michael G. Bausher</name><name sortKey="Hyndman, Scott E" sort="Hyndman, Scott E" uniqKey="Hyndman S" first="Scott E." last="Hyndman">Scott E. Hyndman</name><name sortKey="Wynn, Eldridge T" sort="Wynn, Eldridge T" uniqKey="Wynn E" first="Eldridge T." last="Wynn">Eldridge T. Wynn</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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