OrangerV1, PascalFrancis, Curation, bibRecord, 000216

Normalizing sweet orange (C. sinensis (L.) Osbeck) somatic embryogenesis with semi-permeable membranes

Identifieur interne : 000216 ( PascalFrancis/Curation ); précédent : 000215; suivant : 000217

Normalizing sweet orange (C. sinensis (L.) Osbeck) somatic embryogenesis with semi-permeable membranes

Auteurs : Randall P. Niedz [États-Unis] ; Scott E. Hyndman [États-Unis] ; Eldridge T. Wynn [États-Unis] ; Michael G. Bausher [États-Unis]

Source :

In vitro cellular & developmental biology. Plant [ 1054-5476 ] ; 2002.

RBID : Pascal:03-0096699

Descripteurs français

Pascal (Inist)
- Embryon somatique, Développement embryonnaire, Organogenèse, Morphogenèse, Protocole expérimental, Cellulose acétate, Milieu culture, Potentiel hydrique, Citrus sinensis, Glycérol, Cellulose, Acétate, Saccharose, Gibbérellique acide, Membrane semi perméable.
Wicri :
- topic : Cellulose, Saccharose.

English descriptors

KwdEn :
- Acetate, Cellulose, Cellulose acetate, Citrus sinensis, Culture medium, Embryonic development, Experimental protocol, Gibberellic acid, Glycerol, Morphogenesis, Organogenesis, Somatic embryo, Sucrose, Water potential.

Abstract

Development of citrus somatic embryos initiated from embryogenic callus generally results in abnormal morphogenesis of somatic embryos. To normalize development, glycerol-induced globular-stage somatic embryos of sweet orange [C. sinensis (L.) Osbeck cv. 'Hamlin'] were cultured on 6000-8000MW cutoff cellulose acetate, >400000MW cutoff cellulose acetate, nitrocellulose, polyvinylidene fluoride (PVDF), cellulose filter paper, or positive or neutral charged nylon membranes. Only the two cellulose acetate membranes resulted in the development of normal, two-cotyledon, bipolar, heart-shaped embryos, and no aberrant teratoma-like structures. Heart-shaped embryos developed and germinated normally on Murashige and Tucker basal medium with 0.5% sucrose and 1 μM gibberellic acid. Culture of embryogenic callus directly onto cellulose membranes also resulted in the development of normal heart-shaped embryos, indicating that glycerol induction of globular-stage embryos is not necessary. Heart-shaped embryos were not observed when the osmotic potential of the medium was increased by the addition of 2.5-15% polyethylene glycol; neither were they observed when the matric potential of the medium was increased by increasing the gelling agent concentrations of agar and Gelrite from 0.8% to 3% and 0.15% to 0.9%, respectively.

A01	`01`	`1`		`@0 1054-5476`
A03		`1`		`@0 In vitro cell. dev. biol., Plant`
A05				`@2 38`
A06				`@2 6`
A08	`01`	`1`	`ENG`	`@1 Normalizing sweet orange (C. sinensis (L.) Osbeck) somatic embryogenesis with semi-permeable membranes`
A11	`01`	`1`		`@1 NIEDZ (Randall P.)`
A11	`02`	`1`		`@1 HYNDMAN (Scott E.)`
A11	`03`	`1`		`@1 WYNN (Eldridge T.)`
A11	`04`	`1`		`@1 BAUSHER (Michael G.)`
A14	`01`			`@1 Agricultural Research Service, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Ft. @2 Pierce, FL 34945-3030 @3 USA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut.`
A20				`@1 552-557`
A21				`@1 2002`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 15474A @5 354000105746800050`
A44				`@0 0000 @1 © 2003 INIST-CNRS. All rights reserved.`
A45				`@0 32 ref.`
A47	`01`	`1`		`@0 03-0096699`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 In vitro cellular & developmental biology. Plant`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 Development of citrus somatic embryos initiated from embryogenic callus generally results in abnormal morphogenesis of somatic embryos. To normalize development, glycerol-induced globular-stage somatic embryos of sweet orange [C. sinensis (L.) Osbeck cv. 'Hamlin'] were cultured on 6000-8000MW cutoff cellulose acetate, >400000MW cutoff cellulose acetate, nitrocellulose, polyvinylidene fluoride (PVDF), cellulose filter paper, or positive or neutral charged nylon membranes. Only the two cellulose acetate membranes resulted in the development of normal, two-cotyledon, bipolar, heart-shaped embryos, and no aberrant teratoma-like structures. Heart-shaped embryos developed and germinated normally on Murashige and Tucker basal medium with 0.5% sucrose and 1 μM gibberellic acid. Culture of embryogenic callus directly onto cellulose membranes also resulted in the development of normal heart-shaped embryos, indicating that glycerol induction of globular-stage embryos is not necessary. Heart-shaped embryos were not observed when the osmotic potential of the medium was increased by the addition of 2.5-15% polyethylene glycol; neither were they observed when the matric potential of the medium was increased by increasing the gelling agent concentrations of agar and Gelrite from 0.8% to 3% and 0.15% to 0.9%, respectively.
C02	`01`	`X`		`@0 002A10I`
C02	`02`	`X`		`@0 002A32E09`
C03	`01`	`X`	`FRE`	`@0 Embryon somatique @5 01`
C03	`01`	`X`	`ENG`	`@0 Somatic embryo @5 01`
C03	`01`	`X`	`SPA`	`@0 Embrión somático @5 01`
C03	`02`	`X`	`FRE`	`@0 Développement embryonnaire @5 02`
C03	`02`	`X`	`ENG`	`@0 Embryonic development @5 02`
C03	`02`	`X`	`SPA`	`@0 Desarrollo embrionario @5 02`
C03	`03`	`X`	`FRE`	`@0 Organogenèse @5 03`
C03	`03`	`X`	`ENG`	`@0 Organogenesis @5 03`
C03	`03`	`X`	`SPA`	`@0 Organogénesis @5 03`
C03	`04`	`X`	`FRE`	`@0 Morphogenèse @5 04`
C03	`04`	`X`	`ENG`	`@0 Morphogenesis @5 04`
C03	`04`	`X`	`SPA`	`@0 Morfogénesis @5 04`
C03	`05`	`X`	`FRE`	`@0 Protocole expérimental @5 05`
C03	`05`	`X`	`ENG`	`@0 Experimental protocol @5 05`
C03	`05`	`X`	`SPA`	`@0 Protocolo experimental @5 05`
C03	`06`	`X`	`FRE`	`@0 Cellulose acétate @2 NK @5 06`
C03	`06`	`X`	`ENG`	`@0 Cellulose acetate @2 NK @5 06`
C03	`06`	`X`	`SPA`	`@0 Celulosa acetato @2 NK @5 06`
C03	`07`	`X`	`FRE`	`@0 Milieu culture @5 07`
C03	`07`	`X`	`ENG`	`@0 Culture medium @5 07`
C03	`07`	`X`	`SPA`	`@0 Medio cultivo @5 07`
C03	`08`	`X`	`FRE`	`@0 Potentiel hydrique @5 08`
C03	`08`	`X`	`ENG`	`@0 Water potential @5 08`
C03	`08`	`X`	`SPA`	`@0 Potencial hídrico @5 08`
C03	`09`	`X`	`FRE`	`@0 Citrus sinensis @2 NS @5 10`
C03	`09`	`X`	`ENG`	`@0 Citrus sinensis @2 NS @5 10`
C03	`09`	`X`	`SPA`	`@0 Citrus sinensis @2 NS @5 10`
C03	`10`	`X`	`FRE`	`@0 Glycérol @2 NK @2 FR @5 15`
C03	`10`	`X`	`ENG`	`@0 Glycerol @2 NK @2 FR @5 15`
C03	`10`	`X`	`SPA`	`@0 Glicerol @2 NK @2 FR @5 15`
C03	`11`	`X`	`FRE`	`@0 Cellulose @2 NK @5 16`
C03	`11`	`X`	`ENG`	`@0 Cellulose @2 NK @5 16`
C03	`11`	`X`	`SPA`	`@0 Celulosa @2 NK @5 16`
C03	`12`	`X`	`FRE`	`@0 Acétate @2 NA @5 17`
C03	`12`	`X`	`ENG`	`@0 Acetate @2 NA @5 17`
C03	`12`	`X`	`SPA`	`@0 Acetato @2 NA @5 17`
C03	`13`	`X`	`FRE`	`@0 Saccharose @2 NK @2 FR @5 18`
C03	`13`	`X`	`ENG`	`@0 Sucrose @2 NK @2 FR @5 18`
C03	`13`	`X`	`SPA`	`@0 Sacarosa @2 NK @2 FR @5 18`
C03	`14`	`X`	`FRE`	`@0 Gibbérellique acide @2 NK @2 FR @2 FF @5 19`
C03	`14`	`X`	`ENG`	`@0 Gibberellic acid @2 NK @2 FR @2 FF @5 19`
C03	`14`	`X`	`SPA`	`@0 Giberelico ácido @2 NK @2 FR @2 FF @5 19`
C03	`15`	`X`	`FRE`	`@0 Membrane semi perméable @4 INC @5 69`
C07	`01`	`X`	`FRE`	`@0 Rutaceae @2 NS`
C07	`01`	`X`	`ENG`	`@0 Rutaceae @2 NS`
C07	`01`	`X`	`SPA`	`@0 Rutaceae @2 NS`
C07	`02`	`X`	`FRE`	`@0 Dicotyledones @2 NS`
C07	`02`	`X`	`ENG`	`@0 Dicotyledones @2 NS`
C07	`02`	`X`	`SPA`	`@0 Dicotyledones @2 NS`
C07	`03`	`X`	`FRE`	`@0 Angiospermae @2 NS`
C07	`03`	`X`	`ENG`	`@0 Angiospermae @2 NS`
C07	`03`	`X`	`SPA`	`@0 Angiospermae @2 NS`
C07	`04`	`X`	`FRE`	`@0 Spermatophyta @2 NS`
C07	`04`	`X`	`ENG`	`@0 Spermatophyta @2 NS`
C07	`04`	`X`	`SPA`	`@0 Spermatophyta @2 NS`
C07	`05`	`X`	`FRE`	`@0 Multiplication végétative in vitro @5 33`
C07	`05`	`X`	`ENG`	`@0 Micropropagation @5 33`
C07	`05`	`X`	`SPA`	`@0 Multiplicación vegetativa in vitro @5 33`
C07	`06`	`X`	`FRE`	`@0 Agrume @5 39`
C07	`06`	`X`	`ENG`	`@0 Citrus fruit @5 39`
C07	`06`	`X`	`SPA`	`@0 Agrios @5 39`
N21				`@1 055`
N82				`@1 PSI`

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Pascal:03-0096699

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Normalizing sweet orange (C. sinensis (L.) Osbeck) somatic embryogenesis with semi-permeable membranes</title>
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<author><name sortKey="Hyndman, Scott E" sort="Hyndman, Scott E" uniqKey="Hyndman S" first="Scott E." last="Hyndman">Scott E. Hyndman</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Agricultural Research Service, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Ft.</s1>
<s2>Pierce, FL 34945-3030</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
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<author><name sortKey="Wynn, Eldridge T" sort="Wynn, Eldridge T" uniqKey="Wynn E" first="Eldridge T." last="Wynn">Eldridge T. Wynn</name>
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<s2>Pierce, FL 34945-3030</s2>
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<author><name sortKey="Bausher, Michael G" sort="Bausher, Michael G" uniqKey="Bausher M" first="Michael G." last="Bausher">Michael G. Bausher</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetate</term>
<term>Cellulose</term>
<term>Cellulose acetate</term>
<term>Citrus sinensis</term>
<term>Culture medium</term>
<term>Embryonic development</term>
<term>Experimental protocol</term>
<term>Gibberellic acid</term>
<term>Glycerol</term>
<term>Morphogenesis</term>
<term>Organogenesis</term>
<term>Somatic embryo</term>
<term>Sucrose</term>
<term>Water potential</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Embryon somatique</term>
<term>Développement embryonnaire</term>
<term>Organogenèse</term>
<term>Morphogenèse</term>
<term>Protocole expérimental</term>
<term>Cellulose acétate</term>
<term>Milieu culture</term>
<term>Potentiel hydrique</term>
<term>Citrus sinensis</term>
<term>Glycérol</term>
<term>Cellulose</term>
<term>Acétate</term>
<term>Saccharose</term>
<term>Gibbérellique acide</term>
<term>Membrane semi perméable</term>
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<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Cellulose</term>
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<front><div type="abstract" xml:lang="en">Development of citrus somatic embryos initiated from embryogenic callus generally results in abnormal morphogenesis of somatic embryos. To normalize development, glycerol-induced globular-stage somatic embryos of sweet orange [C. sinensis (L.) Osbeck cv. 'Hamlin'] were cultured on 6000-8000MW cutoff cellulose acetate, >400000MW cutoff cellulose acetate, nitrocellulose, polyvinylidene fluoride (PVDF), cellulose filter paper, or positive or neutral charged nylon membranes. Only the two cellulose acetate membranes resulted in the development of normal, two-cotyledon, bipolar, heart-shaped embryos, and no aberrant teratoma-like structures. Heart-shaped embryos developed and germinated normally on Murashige and Tucker basal medium with 0.5% sucrose and 1 μM gibberellic acid. Culture of embryogenic callus directly onto cellulose membranes also resulted in the development of normal heart-shaped embryos, indicating that glycerol induction of globular-stage embryos is not necessary. Heart-shaped embryos were not observed when the osmotic potential of the medium was increased by the addition of 2.5-15% polyethylene glycol; neither were they observed when the matric potential of the medium was increased by increasing the gelling agent concentrations of agar and Gelrite from 0.8% to 3% and 0.15% to 0.9%, respectively.</div>
</front>
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<fA08 i1="01" i2="1" l="ENG"><s1>Normalizing sweet orange (C. sinensis (L.) Osbeck) somatic embryogenesis with semi-permeable membranes</s1>
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<fA11 i1="01" i2="1"><s1>NIEDZ (Randall P.)</s1>
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<fA11 i1="02" i2="1"><s1>HYNDMAN (Scott E.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>WYNN (Eldridge T.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>BAUSHER (Michael G.)</s1>
</fA11>
<fA14 i1="01"><s1>Agricultural Research Service, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Ft.</s1>
<s2>Pierce, FL 34945-3030</s2>
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<sZ>1 aut.</sZ>
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<fC01 i1="01" l="ENG"><s0>Development of citrus somatic embryos initiated from embryogenic callus generally results in abnormal morphogenesis of somatic embryos. To normalize development, glycerol-induced globular-stage somatic embryos of sweet orange [C. sinensis (L.) Osbeck cv. 'Hamlin'] were cultured on 6000-8000MW cutoff cellulose acetate, >400000MW cutoff cellulose acetate, nitrocellulose, polyvinylidene fluoride (PVDF), cellulose filter paper, or positive or neutral charged nylon membranes. Only the two cellulose acetate membranes resulted in the development of normal, two-cotyledon, bipolar, heart-shaped embryos, and no aberrant teratoma-like structures. Heart-shaped embryos developed and germinated normally on Murashige and Tucker basal medium with 0.5% sucrose and 1 μM gibberellic acid. Culture of embryogenic callus directly onto cellulose membranes also resulted in the development of normal heart-shaped embryos, indicating that glycerol induction of globular-stage embryos is not necessary. Heart-shaped embryos were not observed when the osmotic potential of the medium was increased by the addition of 2.5-15% polyethylene glycol; neither were they observed when the matric potential of the medium was increased by increasing the gelling agent concentrations of agar and Gelrite from 0.8% to 3% and 0.15% to 0.9%, respectively.</s0>
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<fC03 i1="01" i2="X" l="FRE"><s0>Embryon somatique</s0>
<s5>01</s5>
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<fC03 i1="01" i2="X" l="ENG"><s0>Somatic embryo</s0>
<s5>01</s5>
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<fC03 i1="01" i2="X" l="SPA"><s0>Embrión somático</s0>
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<s5>03</s5>
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<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Organogénesis</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Morphogenèse</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Morphogenesis</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Morfogénesis</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Protocole expérimental</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Experimental protocol</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Protocolo experimental</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Cellulose acétate</s0>
<s2>NK</s2>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Cellulose acetate</s0>
<s2>NK</s2>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Celulosa acetato</s0>
<s2>NK</s2>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Milieu culture</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Culture medium</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Medio cultivo</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Potentiel hydrique</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Water potential</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Potencial hídrico</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Glycérol</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Glycerol</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Glicerol</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>15</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Cellulose</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Cellulose</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Celulosa</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Acétate</s0>
<s2>NA</s2>
<s5>17</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Acetate</s0>
<s2>NA</s2>
<s5>17</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Acetato</s0>
<s2>NA</s2>
<s5>17</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Saccharose</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Sucrose</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Sacarosa</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>18</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Gibbérellique acide</s0>
<s2>NK</s2>
<s2>FR</s2>
<s2>FF</s2>
<s5>19</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Gibberellic acid</s0>
<s2>NK</s2>
<s2>FR</s2>
<s2>FF</s2>
<s5>19</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Giberelico ácido</s0>
<s2>NK</s2>
<s2>FR</s2>
<s2>FF</s2>
<s5>19</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Membrane semi perméable</s0>
<s4>INC</s4>
<s5>69</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Multiplication végétative in vitro</s0>
<s5>33</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Micropropagation</s0>
<s5>33</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Multiplicación vegetativa in vitro</s0>
<s5>33</s5>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Agrume</s0>
<s5>39</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>39</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Agrios</s0>
<s5>39</s5>
</fC07>
<fN21><s1>055</s1>
</fN21>
<fN82><s1>PSI</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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   |texte=   Normalizing sweet orange (C. sinensis (L.) Osbeck) somatic embryogenesis with semi-permeable membranes
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Normalizing sweet orange (C. sinensis (L.) Osbeck) somatic embryogenesis with semi-permeable membranes

Normalizing sweet orange (C. sinensis (L.) Osbeck) somatic embryogenesis with semi-permeable membranes

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