Candidatus Liberibacter asiaticus and Huanglongbing Effects on Citrus Seeds and Seedlings
Identifieur interne : 002039 ( Main/Merge ); précédent : 002038; suivant : 002040Candidatus Liberibacter asiaticus and Huanglongbing Effects on Citrus Seeds and Seedlings
Auteurs : Ute Albrecht [États-Unis] ; Kim D. Bowman [États-Unis]Source :
- HortScience [ 0018-5345 ] ; 2009.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Semence, Horticulture.
English descriptors
- KwdEn :
Abstract
Huanglongbing (HLB) is a devastating disease of citrus and threatens the citrus industry worldwide. The suspected causal agent of the disease is a phloem-limited bacterium of the genus Candidatus Liberibacter transmitted through insect vector or grafting with diseased budwood. Currently, most seed source trees for citrus rootstock propagation are located outdoors and unprotected from disease transmission. In addition, fruit from HLB-affected scion varieties in Florida containing seeds enter the commercial trade and move into other citrus-growing areas. The objective of this study was to determine how Ca. L. asiaticus infection affects seed quality and seedling development and whether the disease appears in seedlings grown from infected fruit. Two experiments were conducted involving thousands of seedlings produced from seeds from infected rootstock seed source trees and 'Valencia' sweet orange trees, respectively. Infection of trees and fruit with Ca. L. asiaticus significantly reduced seed weight, seed germination, and seedling height. Seedlings did not develop symptoms typical of HLB throughout the experiment. Polymerase chain reaction (PCR) analysis initially identified two of 686 rootstock seedlings and three of 431 sweet orange seedlings positive for the pathogen when they were very young. Resampling and PCR analysis of these five seedlings at older ages consistently indicated they were negative for the pathogen and none of these plants ever developed symptoms of HLB. It is suggested that Ca. L. asiaticus may have been translocated into some part of the embryo during seed development but that it was not present in cells or tissue, which permitted replication or disease development as the seedling grew.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Candidatus Liberibacter asiaticus and Huanglongbing Effects on Citrus Seeds and Seedlings</title><author><name sortKey="Albrecht, Ute" sort="Albrecht, Ute" uniqKey="Albrecht U" first="Ute" last="Albrecht">Ute Albrecht</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>U.S. Horticultural Research Laboratory, U.S. Department of Agriculture, Agricultural Research Service, 2001 South Rock Road</s1><s2>Fort Pierce, FL 34945</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Bowman, Kim D" sort="Bowman, Kim D" uniqKey="Bowman K" first="Kim D." last="Bowman">Kim D. Bowman</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>U.S. Horticultural Research Laboratory, U.S. Department of Agriculture, Agricultural Research Service, 2001 South Rock Road</s1><s2>Fort Pierce, FL 34945</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">10-0047589</idno><date when="2009">2009</date><idno type="stanalyst">PASCAL 10-0047589 INIST</idno><idno type="RBID">Pascal:10-0047589</idno><idno type="wicri:Area/PascalFrancis/Corpus">000274</idno><idno type="wicri:Area/PascalFrancis/Curation">000726</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000338</idno><idno type="wicri:doubleKey">0018-5345:2009:Albrecht U:candidatus:liberibacter:asiaticus</idno><idno type="wicri:Area/Main/Merge">002039</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Candidatus Liberibacter asiaticus and Huanglongbing Effects on Citrus Seeds and Seedlings</title><author><name sortKey="Albrecht, Ute" sort="Albrecht, Ute" uniqKey="Albrecht U" first="Ute" last="Albrecht">Ute Albrecht</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>U.S. Horticultural Research Laboratory, U.S. Department of Agriculture, Agricultural Research Service, 2001 South Rock Road</s1><s2>Fort Pierce, FL 34945</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Bowman, Kim D" sort="Bowman, Kim D" uniqKey="Bowman K" first="Kim D." last="Bowman">Kim D. Bowman</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>U.S. Horticultural Research Laboratory, U.S. Department of Agriculture, Agricultural Research Service, 2001 South Rock Road</s1><s2>Fort Pierce, FL 34945</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author></analytic><series><title level="j" type="main">HortScience</title><title level="j" type="abbreviated">HortScience</title><idno type="ISSN">0018-5345</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">HortScience</title><title level="j" type="abbreviated">HortScience</title><idno type="ISSN">0018-5345</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus sinensis</term><term>Genotype</term><term>Greening</term><term>Growth</term><term>Horticulture</term><term>Plant juvenile growth stage</term><term>Quality</term><term>Rootstock</term><term>Seed</term><term>Sporophyte</term><term>Transmission</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Stade juvénile plante</term><term>Verdissement</term><term>Porte greffe</term><term>Génotype</term><term>Semence</term><term>Qualité</term><term>Croissance</term><term>Transmission</term><term>Horticulture</term><term>Citrus sinensis</term><term>Sporophyte</term><term>Liberibacter asiaticus</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Semence</term><term>Horticulture</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Huanglongbing (HLB) is a devastating disease of citrus and threatens the citrus industry worldwide. The suspected causal agent of the disease is a phloem-limited bacterium of the genus Candidatus Liberibacter transmitted through insect vector or grafting with diseased budwood. Currently, most seed source trees for citrus rootstock propagation are located outdoors and unprotected from disease transmission. In addition, fruit from HLB-affected scion varieties in Florida containing seeds enter the commercial trade and move into other citrus-growing areas. The objective of this study was to determine how Ca. L. asiaticus infection affects seed quality and seedling development and whether the disease appears in seedlings grown from infected fruit. Two experiments were conducted involving thousands of seedlings produced from seeds from infected rootstock seed source trees and 'Valencia' sweet orange trees, respectively. Infection of trees and fruit with Ca. L. asiaticus significantly reduced seed weight, seed germination, and seedling height. Seedlings did not develop symptoms typical of HLB throughout the experiment. Polymerase chain reaction (PCR) analysis initially identified two of 686 rootstock seedlings and three of 431 sweet orange seedlings positive for the pathogen when they were very young. Resampling and PCR analysis of these five seedlings at older ages consistently indicated they were negative for the pathogen and none of these plants ever developed symptoms of HLB. It is suggested that Ca. L. asiaticus may have been translocated into some part of the embryo during seed development but that it was not present in cells or tissue, which permitted replication or disease development as the seedling grew.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="États-Unis"><region name="Floride"><name sortKey="Albrecht, Ute" sort="Albrecht, Ute" uniqKey="Albrecht U" first="Ute" last="Albrecht">Ute Albrecht</name></region><name sortKey="Bowman, Kim D" sort="Bowman, Kim D" uniqKey="Bowman K" first="Kim D." last="Bowman">Kim D. Bowman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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