Inhibition of Somatic Embryogenesis in Response to 2,3,5-triiodobenzoic acid and 2,4-dichlorophenoxyacetic acid in Ipomoea batatas (L.) Lam. Cultured in vitro
Identifieur interne : 003E85 ( Main/Exploration ); précédent : 003E84; suivant : 003E86Inhibition of Somatic Embryogenesis in Response to 2,3,5-triiodobenzoic acid and 2,4-dichlorophenoxyacetic acid in Ipomoea batatas (L.) Lam. Cultured in vitro
Auteurs : Raymond P. Chée [États-Unis] ; Daniel J. Cantliffe [États-Unis]Source :
- Journal of Plant Physiology [ 0176-1617 ] ; 1989.
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Abstract
Summary: The mechanism by which exogenous auxins inhibit embryo development from embryogenic callus is not known. In sweet potato [Ipomoea batatas, (L.) Lam.] we investigated the hypothesis that embryo development was subjected to endogenous IAA transport by studying embryogenesis from calli in response to various concentrations of either 2,4-dichlorophenoxyacetic acid (2,4-D), 2,3,5-triiodobenzoic acid (TIBA), 7-aza-indole (AZI) or p-chlorophenoxyisobutyric acid (PCIB). Additions of AZI and PCIB at 1 to 5 μM did not affect morphogenesis. Additions of TIBA and 2,4-D at 5 μM inhibited embryo formation and promoted embryogenic callus growth. Embryo development was restricted to increasingly earlier stages, i.e. from the torpedo stage toward the pro-embryo stage, as TIBA was gradually increased from 0 to 6μM, or as 2,4-D was gradually increased from 0 to 5μ/tM. TIBA specifically inhibits polar IAA transport, thus, our results suggest that embryo development may coincide with endogenous IAA transport and that, inhibition of embryogenesis from embryogenic calli by exogenously supplied auxins may be a consequence of the disruption by exogenous auxins of endogenous IAA efflux from embryogenic loci.
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DOI: 10.1016/S0176-1617(89)80094-X
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibition of Somatic Embryogenesis in Response to 2,3,5-triiodobenzoic acid and 2,4-dichlorophenoxyacetic acid in Ipomoea batatas (L.) Lam. Cultured in vitro</title><author><name sortKey="Chee, Raymond P" sort="Chee, Raymond P" uniqKey="Chee R" first="Raymond P." last="Chée">Raymond P. Chée</name></author><author><name sortKey="Cantliffe, Daniel J" sort="Cantliffe, Daniel J" uniqKey="Cantliffe D" first="Daniel J." last="Cantliffe">Daniel J. 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Cultured in vitro</title><author><name sortKey="Chee, Raymond P" sort="Chee, Raymond P" uniqKey="Chee R" first="Raymond P." last="Chée">Raymond P. Chée</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Vegetable Crops Department, University of Florida, Institute of Food and Agricultural Sciences, Gainesville, Florida 32611</wicri:regionArea><wicri:noRegion>Florida 32611</wicri:noRegion></affiliation></author><author><name sortKey="Cantliffe, Daniel J" sort="Cantliffe, Daniel J" uniqKey="Cantliffe D" first="Daniel J." last="Cantliffe">Daniel J. Cantliffe</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Vegetable Crops Department, University of Florida, Institute of Food and Agricultural Sciences, Gainesville, Florida 32611</wicri:regionArea><wicri:noRegion>Florida 32611</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Plant Physiology</title><title level="j" type="abbrev">JPLPH</title><idno type="ISSN">0176-1617</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989">1989</date><biblScope unit="volume">135</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="398">398</biblScope><biblScope unit="page" to="403">403</biblScope></imprint><idno type="ISSN">0176-1617</idno></series><idno type="istex">A5BF8EDC6F97E6EB6A5EFD3904C62DF527571952</idno><idno type="DOI">10.1016/S0176-1617(89)80094-X</idno><idno type="PII">S0176-1617(89)80094-X</idno><idno type="ArticleID">80094</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0176-1617</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>2,4,6-T</term><term>2,4-D</term><term>AZI</term><term>PCIB</term><term>Sweet potato</term><term>TIBA</term><term>auxin polar transport</term><term>polarity</term><term>somatic embryos</term><term>teratomata</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: The mechanism by which exogenous auxins inhibit embryo development from embryogenic callus is not known. In sweet potato [Ipomoea batatas, (L.) Lam.] we investigated the hypothesis that embryo development was subjected to endogenous IAA transport by studying embryogenesis from calli in response to various concentrations of either 2,4-dichlorophenoxyacetic acid (2,4-D), 2,3,5-triiodobenzoic acid (TIBA), 7-aza-indole (AZI) or p-chlorophenoxyisobutyric acid (PCIB). Additions of AZI and PCIB at 1 to 5 μM did not affect morphogenesis. Additions of TIBA and 2,4-D at 5 μM inhibited embryo formation and promoted embryogenic callus growth. Embryo development was restricted to increasingly earlier stages, i.e. from the torpedo stage toward the pro-embryo stage, as TIBA was gradually increased from 0 to 6μM, or as 2,4-D was gradually increased from 0 to 5μ/tM. TIBA specifically inhibits polar IAA transport, thus, our results suggest that embryo development may coincide with endogenous IAA transport and that, inhibition of embryogenesis from embryogenic calli by exogenously supplied auxins may be a consequence of the disruption by exogenous auxins of endogenous IAA efflux from embryogenic loci.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Chee, Raymond P" sort="Chee, Raymond P" uniqKey="Chee R" first="Raymond P." last="Chée">Raymond P. Chée</name></noRegion><name sortKey="Cantliffe, Daniel J" sort="Cantliffe, Daniel J" uniqKey="Cantliffe D" first="Daniel J." last="Cantliffe">Daniel J. Cantliffe</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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