Implications of Carotenoid Biosynthetic Genes in Apocarotenoid Formation during the Stigma Development of Crocus sativus and Its Closer Relatives1
Identifieur interne : 002714 ( Main/Exploration ); précédent : 002713; suivant : 002715Implications of Carotenoid Biosynthetic Genes in Apocarotenoid Formation during the Stigma Development of Crocus sativus and Its Closer Relatives1
Auteurs : Raquel Castillo ; José-Antonio Fernández ; Lourdes G Mez-G MezSource :
- Plant Physiology [ 0032-0889 ] ; 2005.
Abstract
Url:
DOI: 10.1104/pp.105.067827
PubMed: 16183835
PubMed Central: 1255987
Affiliations:
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and Its Closer Relatives<xref ref-type="fn" rid="fn1">1</xref>
</title>
<author><name sortKey="Castillo, Raquel" sort="Castillo, Raquel" uniqKey="Castillo R" first="Raquel" last="Castillo">Raquel Castillo</name>
</author>
<author><name sortKey="Fernandez, Jose Antonio" sort="Fernandez, Jose Antonio" uniqKey="Fernandez J" first="José-Antonio" last="Fernández">José-Antonio Fernández</name>
</author>
<author><name sortKey="G Mez G Mez, Lourdes" sort="G Mez G Mez, Lourdes" uniqKey="G Mez G Mez L" first="Lourdes" last="G Mez-G Mez">Lourdes G Mez-G Mez</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Implications of Carotenoid Biosynthetic Genes in Apocarotenoid Formation during the Stigma Development of <italic>Crocus sativus</italic>
and Its Closer Relatives<xref ref-type="fn" rid="fn1">1</xref>
</title>
<author><name sortKey="Castillo, Raquel" sort="Castillo, Raquel" uniqKey="Castillo R" first="Raquel" last="Castillo">Raquel Castillo</name>
</author>
<author><name sortKey="Fernandez, Jose Antonio" sort="Fernandez, Jose Antonio" uniqKey="Fernandez J" first="José-Antonio" last="Fernández">José-Antonio Fernández</name>
</author>
<author><name sortKey="G Mez G Mez, Lourdes" sort="G Mez G Mez, Lourdes" uniqKey="G Mez G Mez L" first="Lourdes" last="G Mez-G Mez">Lourdes G Mez-G Mez</name>
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<series><title level="j">Plant Physiology</title>
<idno type="ISSN">0032-0889</idno>
<idno type="eISSN">1532-2548</idno>
<imprint><date when="2005">2005</date>
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<front><div type="abstract" xml:lang="en"><p><italic>Crocus sativus</italic>
is a triploid sterile plant characterized by its long red stigmas, which produce and store significant quantities of the apocarotenoids crocetin and crocin, formed from the oxidative cleavage of zeaxanthin. Here, we investigate the accumulation and the molecular mechanisms that regulate the synthesis of these apocarotenoids during stigma development in <italic>C. sativus</italic>
. We cloned the cDNAs for phytoene synthase, lycopene-<italic>β</italic>
-cyclase, and <italic>β</italic>
-ring hydroxylase from <italic>C. sativus</italic>
. With the transition of yellow undeveloped to red fully developed stigmas, an accumulation of zeaxanthin was observed, accompanying the expression of <italic>CsPSY</italic>
, phytoene desaturase, and <italic>CsLYCb</italic>
, and the massive accumulation of <italic>CsBCH</italic>
and <italic>CsZCD</italic>
transcripts. We analyzed the expression of these two transcripts in relation to zeaxanthin and apocarotenoid accumulation in other Crocus species. We observed that only the relative levels of zeaxanthin in the stigma of each cultivar were correlated with the level of <italic>CsBCH</italic>
transcripts. By contrast, the expression levels of <italic>CsZCD</italic>
were not mirrored by changes in the apocarotenoid content, suggesting that the reaction catalyzed by the CsBCH enzyme could be the limiting step in the formation of saffron apocarotenoids in the stigma tissue. Phylogenetic analysis of the <italic>CsBCH</italic>
intron sequences allowed us to determine the relationships among 19 Crocus species and to identify the closely related diploids of <italic>C. sativus</italic>
. In addition, we examined the levels of the carotenoid and apocarotenoid biosynthetic genes in the triploid <italic>C. sativus</italic>
and its closer relatives to determine whether the quantities of these specific mRNAs were additive or not in <italic>C. sativus</italic>
. Transcript levels in saffron were clearly higher and nonadditive, suggesting that, in the triploid gene, regulatory interactions that produce novel effects on carotenoid biosynthesis genes are involved.</p>
</div>
</front>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Castillo, Raquel" sort="Castillo, Raquel" uniqKey="Castillo R" first="Raquel" last="Castillo">Raquel Castillo</name>
<name sortKey="Fernandez, Jose Antonio" sort="Fernandez, Jose Antonio" uniqKey="Fernandez J" first="José-Antonio" last="Fernández">José-Antonio Fernández</name>
<name sortKey="G Mez G Mez, Lourdes" sort="G Mez G Mez, Lourdes" uniqKey="G Mez G Mez L" first="Lourdes" last="G Mez-G Mez">Lourdes G Mez-G Mez</name>
</noCountry>
</tree>
</affiliations>
</record>
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