High-performance liquid chromatography for the characterization of carotenoids in the new sweet orange (Earlygold) grown in Florida, USA
Identifieur interne : 001036 ( Istex/Curation ); précédent : 001035; suivant : 001037High-performance liquid chromatography for the characterization of carotenoids in the new sweet orange (Earlygold) grown in Florida, USA
Auteurs : H. S. Lee [États-Unis] ; W. S. Castle [États-Unis] ; G. A. Coates [États-Unis]Source :
- Journal of Chromatography A [ 0021-9673 ] ; 2001.
Abstract
High-performance liquid chromatography with photodiode array detection was developed for the separation and identification of carotenoids from a new sweet orange, Earlygold. Carotenoid pigments were extracted using hexane–acetone–ethanol and saponified using 10% methanolic potassium hydroxide. More than 25 carotenoid pigments were separated within 40 min using a ternary gradient (acetonitrile–methanol, methyl tert.-butyl ether and water) elution on a C30 reversed-phase column. The carotenoid pattern of Earlygold was generally similar to the early season Hamlin but with some quantitative differences, especially with violaxanthin. Major carotenoids including violaxanthin, lutein, β-cryptoxanthin, antheraxanthin, luteoxanthin, zeaxanthin, β-carotene, and α-carotene were identified based on on-line spectral data obtained by a photodiode array detector, and comparison to the spectra of the standards and reported values. A numerical notation, the ratio of the peak heights between absorption bands, was also calculated to compare to the literature values.
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DOI: 10.1016/S0021-9673(00)01029-3
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Carotenoid pigments were extracted using hexane–acetone–ethanol and saponified using 10% methanolic potassium hydroxide. More than 25 carotenoid pigments were separated within 40 min using a ternary gradient (acetonitrile–methanol, methyl tert.-butyl ether and water) elution on a C30 reversed-phase column. The carotenoid pattern of Earlygold was generally similar to the early season Hamlin but with some quantitative differences, especially with violaxanthin. Major carotenoids including violaxanthin, lutein, β-cryptoxanthin, antheraxanthin, luteoxanthin, zeaxanthin, β-carotene, and α-carotene were identified based on on-line spectral data obtained by a photodiode array detector, and comparison to the spectra of the standards and reported values. A numerical notation, the ratio of the peak heights between absorption bands, was also calculated to compare to the literature values.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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