High-performance liquid chromatography for the characterization of carotenoids in the new sweet orange (Earlygold) grown in Florida, USA
Identifieur interne : 001036 ( Istex/Corpus ); précédent : 001035; suivant : 001037High-performance liquid chromatography for the characterization of carotenoids in the new sweet orange (Earlygold) grown in Florida, USA
Auteurs : H. S. Lee ; W. S. Castle ; G. A. CoatesSource :
- Journal of Chromatography A [ 0021-9673 ] ; 2001.
Abstract
High-performance liquid chromatography with photodiode array detection was developed for the separation and identification of carotenoids from a new sweet orange, Earlygold. Carotenoid pigments were extracted using hexane–acetone–ethanol and saponified using 10% methanolic potassium hydroxide. More than 25 carotenoid pigments were separated within 40 min using a ternary gradient (acetonitrile–methanol, methyl tert.-butyl ether and water) elution on a C30 reversed-phase column. The carotenoid pattern of Earlygold was generally similar to the early season Hamlin but with some quantitative differences, especially with violaxanthin. Major carotenoids including violaxanthin, lutein, β-cryptoxanthin, antheraxanthin, luteoxanthin, zeaxanthin, β-carotene, and α-carotene were identified based on on-line spectral data obtained by a photodiode array detector, and comparison to the spectra of the standards and reported values. A numerical notation, the ratio of the peak heights between absorption bands, was also calculated to compare to the literature values.
Url:
DOI: 10.1016/S0021-9673(00)01029-3
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>High-performance liquid chromatography for the characterization of carotenoids in the new sweet orange (Earlygold) grown in Florida, USA</title><author><name sortKey="Lee, H S" sort="Lee, H S" uniqKey="Lee H" first="H. S." last="Lee">H. S. Lee</name><affiliation><mods:affiliation>E-mail: hsl@lal.ufl.edu</mods:affiliation></affiliation><affiliation><mods:affiliation>Florida Department of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</mods:affiliation></affiliation></author><author><name sortKey="Castle, W S" sort="Castle, W S" uniqKey="Castle W" first="W. S." last="Castle">W. S. Castle</name><affiliation><mods:affiliation>University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</mods:affiliation></affiliation></author><author><name sortKey="Coates, G A" sort="Coates, G A" uniqKey="Coates G" first="G. A." last="Coates">G. 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Lee</name><affiliation><mods:affiliation>E-mail: hsl@lal.ufl.edu</mods:affiliation></affiliation><affiliation><mods:affiliation>Florida Department of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</mods:affiliation></affiliation></author><author><name sortKey="Castle, W S" sort="Castle, W S" uniqKey="Castle W" first="W. S." last="Castle">W. S. Castle</name><affiliation><mods:affiliation>University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</mods:affiliation></affiliation></author><author><name sortKey="Coates, G A" sort="Coates, G A" uniqKey="Coates G" first="G. A." last="Coates">G. A. 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Carotenoid pigments were extracted using hexane–acetone–ethanol and saponified using 10% methanolic potassium hydroxide. More than 25 carotenoid pigments were separated within 40 min using a ternary gradient (acetonitrile–methanol, methyl tert.-butyl ether and water) elution on a C30 reversed-phase column. The carotenoid pattern of Earlygold was generally similar to the early season Hamlin but with some quantitative differences, especially with violaxanthin. Major carotenoids including violaxanthin, lutein, β-cryptoxanthin, antheraxanthin, luteoxanthin, zeaxanthin, β-carotene, and α-carotene were identified based on on-line spectral data obtained by a photodiode array detector, and comparison to the spectra of the standards and reported values. A numerical notation, the ratio of the peak heights between absorption bands, was also calculated to compare to the literature values.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>H.S. Lee</name><affiliations><json:string>E-mail: hsl@lal.ufl.edu</json:string><json:string>Florida Department of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</json:string></affiliations></json:item><json:item><name>W.S. Castle</name><affiliations><json:string>University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</json:string></affiliations></json:item><json:item><name>G.A. Coates</name><affiliations><json:string>Florida Department of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Fruit juices</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Food analysis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Carotenoids</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>High-performance liquid chromatography with photodiode array detection was developed for the separation and identification of carotenoids from a new sweet orange, Earlygold. Carotenoid pigments were extracted using hexane–acetone–ethanol and saponified using 10% methanolic potassium hydroxide. More than 25 carotenoid pigments were separated within 40 min using a ternary gradient (acetonitrile–methanol, methyl tert.-butyl ether and water) elution on a C30 reversed-phase column. The carotenoid pattern of Earlygold was generally similar to the early season Hamlin but with some quantitative differences, especially with violaxanthin. Major carotenoids including violaxanthin, lutein, β-cryptoxanthin, antheraxanthin, luteoxanthin, zeaxanthin, β-carotene, and α-carotene were identified based on on-line spectral data obtained by a photodiode array detector, and comparison to the spectra of the standards and reported values. 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Yeung</name></json:item></editor><pages><last>377</last><first>371</first></pages><conference><json:item><name>International Symposium on High Performance Liquid Phase Separations and Related Techniques. Part I, Seattle, WA, USA HPLC 2000 Part 1 S.I. 20000624 20000630</name></json:item></conference><issn><json:string>0021-9673</json:string></issn><issue>1–2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Journal of Chromatography A</title><publicationDate>2001</publicationDate></host><categories><wos><json:string>CHEMISTRY, ANALYTICAL</json:string><json:string>BIOCHEMICAL RESEARCH METHODS</json:string></wos></categories><publicationDate>2001</publicationDate><copyrightDate>2001</copyrightDate><doi><json:string>10.1016/S0021-9673(00)01029-3</json:string></doi><id>E6C4566431EB8BFBE40B9FEB30E075762A24D235</id><score>0.20822568</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/E6C4566431EB8BFBE40B9FEB30E075762A24D235/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/E6C4566431EB8BFBE40B9FEB30E075762A24D235/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/E6C4566431EB8BFBE40B9FEB30E075762A24D235/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">High-performance liquid chromatography for the characterization of carotenoids in the new sweet orange (Earlygold) grown in Florida, USA</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2001 Elsevier Science B.V.</p></availability><date>2001</date></publicationStmt><notesStmt><note type="content">Fig. 1: RP-HPLC chromatogram for carotenoid in Earlygold orange juice (mid-January, 1997–1998 season). YMC C30 Carotenoid column (4.6×150 mm, 3 μm). Mobile phases are MeCN–MeOH (75:25), MTBE and water. The mobile phase contained 0.01% BHT and 0.05% TEA as modifiers. Detection at 450 nm. See Table 1 for identification.</note><note type="content">Fig. 2: Spectral fine structure (as %III/II) of violaxanthin.</note><note type="content">Table 1: Chromatographic and spectroscopic characteristics of Earlygold orange juice carotenoidsa</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">High-performance liquid chromatography for the characterization of carotenoids in the new sweet orange (Earlygold) grown in Florida, USA</title><author xml:id="author-1"><persName><forename type="first">H.S.</forename><surname>Lee</surname></persName><email>hsl@lal.ufl.edu</email><note type="correspondence"><p>Corresponding author. Tel.: +1-863-295-5943; fax: +1-863-295-5920</p></note><affiliation>Florida Department of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</affiliation></author><author xml:id="author-2"><persName><forename type="first">W.S.</forename><surname>Castle</surname></persName><affiliation>University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</affiliation></author><author xml:id="author-3"><persName><forename type="first">G.A.</forename><surname>Coates</surname></persName><affiliation>Florida Department of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</affiliation></author></analytic><monogr><title level="j">Journal of Chromatography A</title><title level="j" type="abbrev">CHROMA</title><idno type="pISSN">0021-9673</idno><idno type="PII">S0021-9673(00)X0628-0</idno><meeting><addName>International Symposium on High Performance Liquid Phase Separations and Related Techniques. Part I, Seattle, WA, USA</addName><addName>HPLC 2000 Part 1 S.I.</addName><date>20000624</date><date>20000630</date></meeting><editor><persName>Edward S. Yeung</persName></editor><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001"></date><biblScope unit="volume">913</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="371">371</biblScope><biblScope unit="page" to="377">377</biblScope></imprint></monogr><idno type="istex">E6C4566431EB8BFBE40B9FEB30E075762A24D235</idno><idno type="DOI">10.1016/S0021-9673(00)01029-3</idno><idno type="PII">S0021-9673(00)01029-3</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2001</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>High-performance liquid chromatography with photodiode array detection was developed for the separation and identification of carotenoids from a new sweet orange, Earlygold. Carotenoid pigments were extracted using hexane–acetone–ethanol and saponified using 10% methanolic potassium hydroxide. More than 25 carotenoid pigments were separated within 40 min using a ternary gradient (acetonitrile–methanol, methyl tert.-butyl ether and water) elution on a C30 reversed-phase column. The carotenoid pattern of Earlygold was generally similar to the early season Hamlin but with some quantitative differences, especially with violaxanthin. Major carotenoids including violaxanthin, lutein, β-cryptoxanthin, antheraxanthin, luteoxanthin, zeaxanthin, β-carotene, and α-carotene were identified based on on-line spectral data obtained by a photodiode array detector, and comparison to the spectra of the standards and reported values. A numerical notation, the ratio of the peak heights between absorption bands, was also calculated to compare to the literature values.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Fruit juices</term></item><item><term>Food analysis</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Carotenoids</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2001">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/E6C4566431EB8BFBE40B9FEB30E075762A24D235/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="GR1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="GR2"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CHROMA</jid><aid>4905</aid><ce:pii>S0021-9673(00)01029-3</ce:pii><ce:doi>10.1016/S0021-9673(00)01029-3</ce:doi><ce:copyright type="full-transfer" year="2001">Elsevier Science B.V.</ce:copyright></item-info><head><ce:title>High-performance liquid chromatography for the characterization of carotenoids in the new sweet orange (Earlygold) grown in Florida, USA</ce:title><ce:author-group><ce:author><ce:given-name>H.S.</ce:given-name><ce:surname>Lee</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref><ce:e-address>hsl@lal.ufl.edu</ce:e-address></ce:author><ce:author><ce:given-name>W.S.</ce:given-name><ce:surname>Castle</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>G.A.</ce:given-name><ce:surname>Coates</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Florida Department of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +1-863-295-5943; fax: +1-863-295-5920</ce:text></ce:correspondence></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>High-performance liquid chromatography with photodiode array detection was developed for the separation and identification of carotenoids from a new sweet orange, Earlygold. Carotenoid pigments were extracted using hexane–acetone–ethanol and saponified using 10% methanolic potassium hydroxide. More than 25 carotenoid pigments were separated within 40 min using a ternary gradient (acetonitrile–methanol, methyl <ce:italic>tert.</ce:italic>-butyl ether and water) elution on a C<ce:inf>30</ce:inf> reversed-phase column. The carotenoid pattern of Earlygold was generally similar to the early season Hamlin but with some quantitative differences, especially with violaxanthin. Major carotenoids including violaxanthin, lutein, <ce:italic>β</ce:italic>-cryptoxanthin, antheraxanthin, luteoxanthin, zeaxanthin, <ce:italic>β</ce:italic>-carotene, and <ce:italic>α</ce:italic>-carotene were identified based on on-line spectral data obtained by a photodiode array detector, and comparison to the spectra of the standards and reported values. A numerical notation, the ratio of the peak heights between absorption bands, was also calculated to compare to the literature values.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="idt"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Fruit juices</ce:text></ce:keyword><ce:keyword><ce:text>Food analysis</ce:text></ce:keyword></ce:keywords><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Carotenoids</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>High-performance liquid chromatography for the characterization of carotenoids in the new sweet orange (Earlygold) grown in Florida, USA</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>High-performance liquid chromatography for the characterization of carotenoids in the new sweet orange (Earlygold) grown in Florida, USA</title></titleInfo><name type="personal"><namePart type="given">H.S.</namePart><namePart type="family">Lee</namePart><affiliation>E-mail: hsl@lal.ufl.edu</affiliation><affiliation>Florida Department of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</affiliation><description>Corresponding author. Tel.: +1-863-295-5943; fax: +1-863-295-5920</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.S.</namePart><namePart type="family">Castle</namePart><affiliation>University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.A.</namePart><namePart type="family">Coates</namePart><affiliation>Florida Department of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">High-performance liquid chromatography with photodiode array detection was developed for the separation and identification of carotenoids from a new sweet orange, Earlygold. Carotenoid pigments were extracted using hexane–acetone–ethanol and saponified using 10% methanolic potassium hydroxide. More than 25 carotenoid pigments were separated within 40 min using a ternary gradient (acetonitrile–methanol, methyl tert.-butyl ether and water) elution on a C30 reversed-phase column. The carotenoid pattern of Earlygold was generally similar to the early season Hamlin but with some quantitative differences, especially with violaxanthin. Major carotenoids including violaxanthin, lutein, β-cryptoxanthin, antheraxanthin, luteoxanthin, zeaxanthin, β-carotene, and α-carotene were identified based on on-line spectral data obtained by a photodiode array detector, and comparison to the spectra of the standards and reported values. A numerical notation, the ratio of the peak heights between absorption bands, was also calculated to compare to the literature values.</abstract><note type="content">Fig. 1: RP-HPLC chromatogram for carotenoid in Earlygold orange juice (mid-January, 1997–1998 season). YMC C30 Carotenoid column (4.6×150 mm, 3 μm). Mobile phases are MeCN–MeOH (75:25), MTBE and water. The mobile phase contained 0.01% BHT and 0.05% TEA as modifiers. Detection at 450 nm. See Table 1 for identification.</note><note type="content">Fig. 2: Spectral fine structure (as %III/II) of violaxanthin.</note><note type="content">Table 1: Chromatographic and spectroscopic characteristics of Earlygold orange juice carotenoidsa</note><subject><genre>Keywords</genre><topic>Fruit juices</topic><topic>Food analysis</topic></subject><subject><genre>Keywords</genre><topic>Carotenoids</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Chromatography A</title></titleInfo><titleInfo type="abbreviated"><title>CHROMA</title></titleInfo><name type="conference"><namePart>International Symposium on High Performance Liquid Phase Separations and Related Techniques. Part I, Seattle, WA, USA</namePart><namePart>HPLC 2000 Part 1 S.I.</namePart><namePart type="date">20000624</namePart><namePart type="date">20000630</namePart></name><name type="personal"><namePart>Edward S. 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