Serological characterisation of citrus tristeza virus isolates from Israel
Identifieur interne : 001319 ( Istex/Corpus ); précédent : 001318; suivant : 001320Serological characterisation of citrus tristeza virus isolates from Israel
Auteurs : D. Shalitin ; M. Mawassi ; R. Gafny ; O. Leitner ; S. Cabilly ; Z. Eshhar ; M. Bar-JosephSource :
- Annals of Applied Biology [ 0003-4746 ] ; 1994-08.
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Abstract
Seven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of citrus tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reactions in ELISA with polyclonal antibodies and with one MAb (#25#2). Two MAbs; #13#21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG‐VT group (Mawassi, Gafny & Bar‐Joseph, 1993), reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG‐MT group, did not react with five or more of the MAbs. These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV‐CPG.
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DOI: 10.1111/j.1744-7348.1994.tb04951.x
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ISTEX:04A0F32E5C8FF11668E00CC56B23B59EE2F91FF6Le document en format XML
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Shalitin</name><affiliation><mods:affiliation>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</mods:affiliation></affiliation></author><author><name sortKey="Mawassi, M" sort="Mawassi, M" uniqKey="Mawassi M" first="M" last="Mawassi">M. Mawassi</name><affiliation><mods:affiliation>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</mods:affiliation></affiliation></author><author><name sortKey="Gafny, R" sort="Gafny, R" uniqKey="Gafny R" first="R" last="Gafny">R. Gafny</name><affiliation><mods:affiliation>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</mods:affiliation></affiliation></author><author><name sortKey="Leitner, O" sort="Leitner, O" uniqKey="Leitner O" first="O" last="Leitner">O. Leitner</name><affiliation><mods:affiliation>Department of Biological Sciences, The Weizman Institute of Science, Rehovot, Israel</mods:affiliation></affiliation></author><author><name sortKey="Cabilly, S" sort="Cabilly, S" uniqKey="Cabilly S" first="S" last="Cabilly">S. Cabilly</name><affiliation><mods:affiliation>Department of Membrane and Biophysics, The Weizman Institute of Science, Rehovot, Israel</mods:affiliation></affiliation></author><author><name sortKey="Eshhar, Z" sort="Eshhar, Z" uniqKey="Eshhar Z" first="Z" last="Eshhar">Z. Eshhar</name><affiliation><mods:affiliation>Department of Chemical Immunology, The Weizman Institute of Science, Rehovot, Israel</mods:affiliation></affiliation></author><author><name sortKey="Bar Oseph, M" sort="Bar Oseph, M" uniqKey="Bar Oseph M" first="M" last="Bar-Joseph">M. Bar-Joseph</name><affiliation><mods:affiliation>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Annals of Applied Biology</title><idno type="ISSN">0003-4746</idno><idno type="eISSN">1744-7348</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1994-08">1994-08</date><biblScope unit="volume">125</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="105">105</biblScope><biblScope unit="page" to="113">113</biblScope></imprint><idno type="ISSN">0003-4746</idno></series><idno type="istex">04A0F32E5C8FF11668E00CC56B23B59EE2F91FF6</idno><idno type="DOI">10.1111/j.1744-7348.1994.tb04951.x</idno><idno type="ArticleID">AAB105</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-4746</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Monoclonal antibodies</term><term>Tristeza‐epidemiology</term><term>aphid transmissibility</term><term>coat protein gene</term><term>pathogenic isolates</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Seven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of citrus tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reactions in ELISA with polyclonal antibodies and with one MAb (#25#2). Two MAbs; #13#21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG‐VT group (Mawassi, Gafny & Bar‐Joseph, 1993), reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG‐MT group, did not react with five or more of the MAbs. These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV‐CPG.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>D SHALITIN</name><affiliations><json:string>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</json:string></affiliations></json:item><json:item><name>M MAWASSI</name><affiliations><json:string>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</json:string></affiliations></json:item><json:item><name>R GAFNY</name><affiliations><json:string>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</json:string></affiliations></json:item><json:item><name>O LEITNER</name><affiliations><json:string>Department of Biological Sciences, The Weizman Institute of Science, Rehovot, Israel</json:string></affiliations></json:item><json:item><name>S CABILLY</name><affiliations><json:string>Department of Membrane and Biophysics, The Weizman Institute of Science, Rehovot, Israel</json:string></affiliations></json:item><json:item><name>Z ESHHAR</name><affiliations><json:string>Department of Chemical Immunology, The Weizman Institute of Science, Rehovot, Israel</json:string></affiliations></json:item><json:item><name>M BAR‐JOSEPH</name><affiliations><json:string>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Monoclonal antibodies</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>coat protein gene</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Tristeza‐epidemiology</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>pathogenic isolates</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>aphid transmissibility</value></json:item></subject><articleId><json:string>AAB105</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Seven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of citrus tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reactions in ELISA with polyclonal antibodies and with one MAb (#25#2). Two MAbs; #13#21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG‐VT group (Mawassi, Gafny & Bar‐Joseph, 1993), reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG‐MT group, did not react with five or more of the MAbs. These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV‐CPG.</abstract><qualityIndicators><score>5.443</score><pdfVersion>1.3</pdfVersion><pdfPageSize>504 x 755.759 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1189</abstractCharCount><pdfWordCount>3223</pdfWordCount><pdfCharCount>19966</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>185</abstractWordCount></qualityIndicators><title>Serological characterisation of citrus tristeza virus isolates from 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ident="en">en</language></langUsage><abstract xml:lang="en"><p>Seven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of citrus tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reactions in ELISA with polyclonal antibodies and with one MAb (#25#2). Two MAbs; #13#21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG‐VT group (Mawassi, Gafny & Bar‐Joseph, 1993), reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG‐MT group, did not react with five or more of the MAbs. These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV‐CPG.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>Monoclonal antibodies</term></item><item><term>coat protein gene</term></item><item><term>Tristeza‐epidemiology</term></item><item><term>pathogenic isolates</term></item><item><term>aphid transmissibility</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1994-08">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/04A0F32E5C8FF11668E00CC56B23B59EE2F91FF6/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" 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countryCode="IL"><unparsedAffiliation>Department of Biological Sciences, The Weizman Institute of Science, Rehovot, Israel</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="IL"><unparsedAffiliation>Department of Membrane and Biophysics, The Weizman Institute of Science, Rehovot, Israel</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="IL"><unparsedAffiliation>Department of Chemical Immunology, The Weizman Institute of Science, Rehovot, Israel</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">Monoclonal antibodies</keyword><keyword xml:id="k2">coat protein gene</keyword><keyword xml:id="k3">Tristeza‐epidemiology</keyword><keyword xml:id="k4">pathogenic isolates</keyword><keyword xml:id="k5">aphid transmissibility</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>Seven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of citrus tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reactions in ELISA with polyclonal antibodies and with one MAb (#25#2). Two MAbs; #13#21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG‐VT group (Mawassi, Gafny & Bar‐Joseph, 1993), reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG‐MT group, did not react with five or more of the MAbs.</p><p>These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV‐CPG.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Serological characterisation of citrus tristeza virus isolates from Israel</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Serological characterisation of citrus tristeza virus isolates from Israel</title></titleInfo><name type="personal"><namePart type="given">D</namePart><namePart type="family">SHALITIN</namePart><affiliation>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">MAWASSI</namePart><affiliation>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R</namePart><namePart type="family">GAFNY</namePart><affiliation>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O</namePart><namePart type="family">LEITNER</namePart><affiliation>Department of Biological Sciences, The Weizman Institute of Science, Rehovot, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">CABILLY</namePart><affiliation>Department of Membrane and Biophysics, The Weizman Institute of Science, Rehovot, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Z</namePart><namePart type="family">ESHHAR</namePart><affiliation>Department of Chemical Immunology, The Weizman Institute of Science, Rehovot, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">BAR‐JOSEPH</namePart><affiliation>The S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1994-08</dateIssued><edition>Accepted 24 February 1994, Received 13 September 1993</edition><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="references">27</extent></physicalDescription><abstract lang="en">Seven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of citrus tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reactions in ELISA with polyclonal antibodies and with one MAb (#25#2). Two MAbs; #13#21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG‐VT group (Mawassi, Gafny & Bar‐Joseph, 1993), reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG‐MT group, did not react with five or more of the MAbs. These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV‐CPG.</abstract><subject lang="en"><genre>keywords</genre><topic>Monoclonal antibodies</topic><topic>coat protein gene</topic><topic>Tristeza‐epidemiology</topic><topic>pathogenic isolates</topic><topic>aphid transmissibility</topic></subject><relatedItem type="host"><titleInfo><title>Annals of Applied Biology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0003-4746</identifier><identifier type="eISSN">1744-7348</identifier><identifier type="DOI">10.1111/(ISSN)1744-7348</identifier><identifier type="PublisherID">AAB</identifier><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>125</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>105</start><end>113</end><total>9</total></extent></part></relatedItem><identifier type="istex">04A0F32E5C8FF11668E00CC56B23B59EE2F91FF6</identifier><identifier type="DOI">10.1111/j.1744-7348.1994.tb04951.x</identifier><identifier type="ArticleID">AAB105</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><enrichments><json:item><type>multicat</type><uri>https://api.istex.fr/document/04A0F32E5C8FF11668E00CC56B23B59EE2F91FF6/enrichments/multicat</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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