Cryopreservation of embryonic axes of trifoliate orange ( Poncirus trifoliata [L.] RAF.)
Identifieur interne : 001167 ( Istex/Checkpoint ); précédent : 001166; suivant : 001168Cryopreservation of embryonic axes of trifoliate orange ( Poncirus trifoliata [L.] RAF.)
Auteurs : J. Radhamani [Inde] ; S. Chandel [Inde]Source :
- Plant Cell Reports [ 0721-7714 ] ; 1992-07-01.
Abstract
Abstract: Seeds of trifoliate orange (Poncirus trifoliata (L.) Raf.) are sensitive to desiccation, and could not withstand reduction in moisture level below 20%, whereas the excised embryonic axes could be easily desiccated to moisture levels as low as 14% without much loss in viability. Axes could be successfully cryopreserved in liquid nitrogen (−196°C) for eight months. The viable embryonic axes exhibited good growth on modified Murashige and Skoog medium supplemented wiith 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP). Growth of cryopreserved axes was promoted in the presence of charcoal in the medium allowing for plant recovery.
Url:
DOI: 10.1007/BF00233369
Affiliations:
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<front><div type="abstract" xml:lang="en">Abstract: Seeds of trifoliate orange (Poncirus trifoliata (L.) Raf.) are sensitive to desiccation, and could not withstand reduction in moisture level below 20%, whereas the excised embryonic axes could be easily desiccated to moisture levels as low as 14% without much loss in viability. Axes could be successfully cryopreserved in liquid nitrogen (−196°C) for eight months. The viable embryonic axes exhibited good growth on modified Murashige and Skoog medium supplemented wiith 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP). Growth of cryopreserved axes was promoted in the presence of charcoal in the medium allowing for plant recovery.</div>
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