Cryopreservation of embryonic axes of trifoliate orange ( Poncirus trifoliata [L.] RAF.)
Identifieur interne : 000E57 ( Istex/Curation ); précédent : 000E56; suivant : 000E58Cryopreservation of embryonic axes of trifoliate orange ( Poncirus trifoliata [L.] RAF.)
Auteurs : J. Radhamani [Inde] ; S. Chandel [Inde]Source :
- Plant Cell Reports [ 0721-7714 ] ; 1992-07-01.
Abstract
Abstract: Seeds of trifoliate orange (Poncirus trifoliata (L.) Raf.) are sensitive to desiccation, and could not withstand reduction in moisture level below 20%, whereas the excised embryonic axes could be easily desiccated to moisture levels as low as 14% without much loss in viability. Axes could be successfully cryopreserved in liquid nitrogen (−196°C) for eight months. The viable embryonic axes exhibited good growth on modified Murashige and Skoog medium supplemented wiith 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP). Growth of cryopreserved axes was promoted in the presence of charcoal in the medium allowing for plant recovery.
Url:
DOI: 10.1007/BF00233369
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000E57
Links to Exploration step
ISTEX:6CD1B37A2713F91BBD111A3FF85DC71061E908D9Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cryopreservation of embryonic axes of trifoliate orange ( Poncirus trifoliata [L.] RAF.)</title><author><name sortKey="Radhamani, J" sort="Radhamani, J" uniqKey="Radhamani J" first="J." last="Radhamani">J. Radhamani</name><affiliation wicri:level="1"><mods:affiliation>National Plant Tissue Culture Repository, NBPGR Campus, 110012, New Delhi, India</mods:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>National Plant Tissue Culture Repository, NBPGR Campus, 110012, New Delhi</wicri:regionArea></affiliation></author><author><name sortKey="Chandel, S" sort="Chandel, S" uniqKey="Chandel S" first="S." last="Chandel">S. Chandel</name><affiliation wicri:level="1"><mods:affiliation>National Plant Tissue Culture Repository, NBPGR Campus, 110012, New Delhi, India</mods:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>National Plant Tissue Culture Repository, NBPGR Campus, 110012, New Delhi</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:6CD1B37A2713F91BBD111A3FF85DC71061E908D9</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1007/BF00233369</idno><idno type="url">https://api.istex.fr/document/6CD1B37A2713F91BBD111A3FF85DC71061E908D9/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000E57</idno><idno type="wicri:Area/Istex/Curation">000E57</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Cryopreservation of embryonic axes of trifoliate orange ( Poncirus trifoliata [L.] RAF.)</title><author><name sortKey="Radhamani, J" sort="Radhamani, J" uniqKey="Radhamani J" first="J." last="Radhamani">J. Radhamani</name><affiliation wicri:level="1"><mods:affiliation>National Plant Tissue Culture Repository, NBPGR Campus, 110012, New Delhi, India</mods:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>National Plant Tissue Culture Repository, NBPGR Campus, 110012, New Delhi</wicri:regionArea></affiliation></author><author><name sortKey="Chandel, S" sort="Chandel, S" uniqKey="Chandel S" first="S." last="Chandel">S. Chandel</name><affiliation wicri:level="1"><mods:affiliation>National Plant Tissue Culture Repository, NBPGR Campus, 110012, New Delhi, India</mods:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>National Plant Tissue Culture Repository, NBPGR Campus, 110012, New Delhi</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Cell Reports</title><title level="j" type="abbrev">Plant Cell Reports</title><idno type="ISSN">0721-7714</idno><idno type="eISSN">1432-203X</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1992-07-01">1992-07-01</date><biblScope unit="volume">11</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="372">372</biblScope><biblScope unit="page" to="374">374</biblScope></imprint><idno type="ISSN">0721-7714</idno></series><idno type="istex">6CD1B37A2713F91BBD111A3FF85DC71061E908D9</idno><idno type="DOI">10.1007/BF00233369</idno><idno type="ArticleID">BF00233369</idno><idno type="ArticleID">Art12</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0721-7714</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Seeds of trifoliate orange (Poncirus trifoliata (L.) Raf.) are sensitive to desiccation, and could not withstand reduction in moisture level below 20%, whereas the excised embryonic axes could be easily desiccated to moisture levels as low as 14% without much loss in viability. Axes could be successfully cryopreserved in liquid nitrogen (−196°C) for eight months. The viable embryonic axes exhibited good growth on modified Murashige and Skoog medium supplemented wiith 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP). Growth of cryopreserved axes was promoted in the presence of charcoal in the medium allowing for plant recovery.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E57 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000E57 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:6CD1B37A2713F91BBD111A3FF85DC71061E908D9
|texte= Cryopreservation of embryonic axes of trifoliate orange ( Poncirus trifoliata [L.] RAF.)
}}
| This area was generated with Dilib version V0.6.25. |  |