NickelMaghrebV1, Main, Exploration, bibRecord, 000072

Immobilization of His-tagged recombinant xylanase from Penicillium occitanis on Nickel-chelate Eupergit C for increasing digestibility of poultry feed

Identifieur interne : 000072 ( Main/Exploration ); précédent : 000071; suivant : 000073

Immobilization of His-tagged recombinant xylanase from Penicillium occitanis on Nickel-chelate Eupergit C for increasing digestibility of poultry feed

Auteurs : Dorra Driss [Tunisie] ; Zied Driss [Tunisie] ; Fatma Chaari [Tunisie] ; Semia Ellouz Chaabouni [Tunisie]

Source :

Bioengineered [ 2165-5979 ] ; 2014.

RBID : PMC:4143399

English descriptors

KwdEn :
- Endo-1,4-beta Xylanases (metabolism), Fungal Proteins (metabolism), Glucuronates (biosynthesis), Oligosaccharides (biosynthesis), Penicillium (enzymology).
MESH :
- chemical , biosynthesis : Glucuronates, Oligosaccharides.
- chemical , metabolism : Endo-1,4-beta Xylanases, Fungal Proteins.
- enzymology : Penicillium.

Abstract

Recombinant xylanase 2 from Penicillium occitanis expressed with an His-tag in Pichia pastoris, termed PoXyn2, was immobilized on nickel-chelate Eupergit C by covalent coupling reaction with a high immobilization yield up to 93.49%. Characterization of the immobilized PoXyn2 was further evaluated. The optimum pH was not affected by immobilization, but the immobilized PoXyn2 exhibited more acidic and large optimum pH range (pH 2.0–4.0) than that of the free PoXyn2 (pH 3.0). The free PoXyn2 had an optimum temperature of 50 °C, whereas that of the immobilized enzyme was shifted to 65 °C. Immobilization increased both pH stability and thermostability when compared with the free enzyme. Thermodynamically, increase in enthalpy and free energy change after covalent immobilization could be credited to the enhanced stability. Immobilized xylanase could be reused for 10 consecutive cycles retaining 60% of its initial activity. It was found to be effective in releasing reducing sugar from poultry feed. Immobilization on Eupergit C is important due to its mechanical resistance at high pH and temperature. Hence, considerable stability and reusability of bound enzyme may be advantageous for its industrial application.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143399

DOI: 10.4161/bioe.29596
PubMed: 24932488
PubMed Central: 4143399

Affiliations:

Tunisie

Le document en format XML

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 on Nickel-chelate Eupergit C for increasing digestibility of poultry feed</title>
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 on Nickel-chelate Eupergit C for increasing digestibility of poultry feed</title>
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<front><div type="abstract" xml:lang="en"><p>Recombinant xylanase 2 from <italic>Penicillium occitanis</italic>
 expressed with an His-tag in <italic>Pichia pastoris</italic>
, termed PoXyn2, was immobilized on nickel-chelate Eupergit C by covalent coupling reaction with a high immobilization yield up to 93.49%. Characterization of the immobilized PoXyn2 was further evaluated. The optimum pH was not affected by immobilization, but the immobilized PoXyn2 exhibited more acidic and large optimum pH range (pH 2.0–4.0) than that of the free PoXyn2 (pH 3.0). The free PoXyn2 had an optimum temperature of 50 °C, whereas that of the immobilized enzyme was shifted to 65 °C. Immobilization increased both pH stability and thermostability when compared with the free enzyme. Thermodynamically, increase in enthalpy and free energy change after covalent immobilization could be credited to the enhanced stability. Immobilized xylanase could be reused for 10 consecutive cycles retaining 60% of its initial activity. It was found to be effective in releasing reducing sugar from poultry feed. Immobilization on Eupergit C is important due to its mechanical resistance at high pH and temperature. Hence, considerable stability and reusability of bound enzyme may be advantageous for its industrial application.</p>
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Serveur d'exploration sur le nickel au Maghreb

Immobilization of His-tagged recombinant xylanase from Penicillium occitanis on Nickel-chelate Eupergit C for increasing digestibility of poultry feed

Immobilization of His-tagged recombinant xylanase from Penicillium occitanis on Nickel-chelate Eupergit C for increasing digestibility of poultry feed

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le nickel au Maghreb
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