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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identifying and tracking proteins through the marine water column: insights into the inputs and preservation mechanisms of protein in sediments</title><author><name sortKey="Moore, Eli K" sort="Moore, Eli K" uniqKey="Moore E" first="Eli K." last="Moore">Eli K. Moore</name><affiliation><nlm:aff id="A1">University of Maryland Center for Environmental Science, Chesapeake Biological Laboratory, Solomons, MD USA</nlm:aff></affiliation></author><author><name sortKey="Nunn, Brook L" sort="Nunn, Brook L" uniqKey="Nunn B" first="Brook L." last="Nunn">Brook L. Nunn</name><affiliation><nlm:aff id="A2">Dept. of Medicinal Chemistry, University of Washington, Seattle, WA USA</nlm:aff></affiliation></author><author><name sortKey="Goodlett, David R" sort="Goodlett, David R" uniqKey="Goodlett D" first="David R." last="Goodlett">David R. Goodlett</name><affiliation><nlm:aff id="A2">Dept. of Medicinal Chemistry, University of Washington, Seattle, WA USA</nlm:aff></affiliation></author><author><name sortKey="Harvey, H Rodger" sort="Harvey, H Rodger" uniqKey="Harvey H" first="H. Rodger" last="Harvey">H. Rodger Harvey</name><affiliation><nlm:aff id="A1">University of Maryland Center for Environmental Science, Chesapeake Biological Laboratory, Solomons, MD USA</nlm:aff></affiliation><affiliation><nlm:aff id="A3">Dept. of Ocean, Earth and Atmospheric Sciences, Old Dominion University, Norfolk, VA USA</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22711915</idno><idno type="pmc">3375732</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375732</idno><idno type="RBID">PMC:3375732</idno><idno type="doi">10.1016/j.gca.2012.01.002</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">000466</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identifying and tracking proteins through the marine water column: insights into the inputs and preservation mechanisms of protein in sediments</title><author><name sortKey="Moore, Eli K" sort="Moore, Eli K" uniqKey="Moore E" first="Eli K." last="Moore">Eli K. Moore</name><affiliation><nlm:aff id="A1">University of Maryland Center for Environmental Science, Chesapeake Biological Laboratory, Solomons, MD USA</nlm:aff></affiliation></author><author><name sortKey="Nunn, Brook L" sort="Nunn, Brook L" uniqKey="Nunn B" first="Brook L." last="Nunn">Brook L. Nunn</name><affiliation><nlm:aff id="A2">Dept. of Medicinal Chemistry, University of Washington, Seattle, WA USA</nlm:aff></affiliation></author><author><name sortKey="Goodlett, David R" sort="Goodlett, David R" uniqKey="Goodlett D" first="David R." last="Goodlett">David R. Goodlett</name><affiliation><nlm:aff id="A2">Dept. of Medicinal Chemistry, University of Washington, Seattle, WA USA</nlm:aff></affiliation></author><author><name sortKey="Harvey, H Rodger" sort="Harvey, H Rodger" uniqKey="Harvey H" first="H. Rodger" last="Harvey">H. Rodger Harvey</name><affiliation><nlm:aff id="A1">University of Maryland Center for Environmental Science, Chesapeake Biological Laboratory, Solomons, MD USA</nlm:aff></affiliation><affiliation><nlm:aff id="A3">Dept. of Ocean, Earth and Atmospheric Sciences, Old Dominion University, Norfolk, VA USA</nlm:aff></affiliation></author></analytic><series><title level="j">Geochimica et cosmochimica acta</title><idno type="ISSN">0016-7037</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Proteins generated during primary production represent an important fraction of marine organic nitrogen and carbon, and have the potential to provide organism-specific information in the environment. The Bering Sea is a highly productive system dominated by seasonal blooms and was used as a model system for algal proteins to be tracked through the water column and incorporated into detrital sedimentary material. Samples of suspended and sinking particles were collected at multiple depths along with surface sediments on the continental shelf and deeper basin of the Bering Sea. Modified standard proteomic preparations were used in conjunction with high pressure liquid chromatography-tandem mass spectrometry to identify the suite of proteins present and monitor changes in their distribution. In surface waters 207 proteins were identified, decreasing through the water column to 52 proteins identified in post-bloom shelf surface sediments and 24 proteins in deeper (3490 m) basin sediments. The vast majority of identified proteins in all samples were diatom in origin, reflecting their dominant contribution of biomass during the spring bloom. Identified proteins were predominantly from metabolic, binding/structural, and transport-related protein groups. Significant linear correlations were observed between the number of proteins identified and the concentration of total hydrolysable amino acids normalized to carbon and nitrogen. Organelle-bound, transmembrane, photosynthetic, and other proteins involved in light harvesting were preferentially retained during recycling. These findings suggest that organelle and membrane protection represent important mechanisms that enhance the preservation of protein during transport and incorporation into sediments.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9876074</journal-id><journal-id journal-id-type="pubmed-jr-id">22847</journal-id><journal-id journal-id-type="nlm-ta">Geochim Cosmochim Acta</journal-id><journal-title-group><journal-title>Geochimica et cosmochimica acta</journal-title></journal-title-group><issn pub-type="ppub">0016-7037</issn></journal-meta><article-meta><article-id pub-id-type="pmid">22711915</article-id><article-id pub-id-type="pmc">3375732</article-id><article-id pub-id-type="doi">10.1016/j.gca.2012.01.002</article-id><article-id pub-id-type="manuscript">NIHMS352104</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Identifying and tracking proteins through the marine water column: insights into the inputs and preservation mechanisms of protein in sediments</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Moore</surname><given-names>Eli K.</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Nunn</surname><given-names>Brook L.</given-names></name><xref ref-type="aff" rid="A2">b</xref></contrib><contrib contrib-type="author"><name><surname>Goodlett</surname><given-names>David R.</given-names></name><xref ref-type="aff" rid="A2">b</xref></contrib><contrib contrib-type="author"><name><surname>Harvey</surname><given-names>H. Rodger</given-names></name><xref ref-type="aff" rid="A1">a</xref><xref ref-type="aff" rid="A3">c</xref><xref ref-type="corresp" rid="CR1">*</xref></contrib></contrib-group><aff id="A1"><label>a</label>University of Maryland Center for Environmental Science, Chesapeake Biological Laboratory, Solomons, MD USA</aff><aff id="A2"><label>b</label>Dept. of Medicinal Chemistry, University of Washington, Seattle, WA USA</aff><aff id="A3"><label>c</label>Dept. of Ocean, Earth and Atmospheric Sciences, Old Dominion University, Norfolk, VA USA</aff><author-notes><corresp id="CR1"><label>*</label>Corresponding author: <email>rharvey@odu.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>8</day><month>2</month><year>2012</year></pub-date><pub-date pub-type="epub"><day>24</day><month>1</month><year>2012</year></pub-date><pub-date pub-type="ppub"><day>15</day><month>4</month><year>2012</year></pub-date><pub-date pub-type="pmc-release"><day>15</day><month>4</month><year>2013</year></pub-date><volume>83</volume><fpage>324</fpage><lpage>359</lpage><permissions><copyright-statement>© 2012 Elsevier Ltd. All rights reserved</copyright-statement><copyright-year>2012</copyright-year></permissions><abstract><p id="P1">Proteins generated during primary production represent an important fraction of marine organic nitrogen and carbon, and have the potential to provide organism-specific information in the environment. The Bering Sea is a highly productive system dominated by seasonal blooms and was used as a model system for algal proteins to be tracked through the water column and incorporated into detrital sedimentary material. Samples of suspended and sinking particles were collected at multiple depths along with surface sediments on the continental shelf and deeper basin of the Bering Sea. Modified standard proteomic preparations were used in conjunction with high pressure liquid chromatography-tandem mass spectrometry to identify the suite of proteins present and monitor changes in their distribution. In surface waters 207 proteins were identified, decreasing through the water column to 52 proteins identified in post-bloom shelf surface sediments and 24 proteins in deeper (3490 m) basin sediments. The vast majority of identified proteins in all samples were diatom in origin, reflecting their dominant contribution of biomass during the spring bloom. Identified proteins were predominantly from metabolic, binding/structural, and transport-related protein groups. Significant linear correlations were observed between the number of proteins identified and the concentration of total hydrolysable amino acids normalized to carbon and nitrogen. Organelle-bound, transmembrane, photosynthetic, and other proteins involved in light harvesting were preferentially retained during recycling. These findings suggest that organelle and membrane protection represent important mechanisms that enhance the preservation of protein during transport and incorporation into sediments.</p></abstract><kwd-group><kwd>proteomics</kwd><kwd>amino acids</kwd><kwd>mass spectrometry</kwd><kwd>marine sediments</kwd><kwd>organic nitrogen</kwd><kwd>peptides</kwd><kwd>sediment traps</kwd><kwd>Bering Sea</kwd><kwd>diatom</kwd><kwd>organelles</kwd><kwd>gel-electrophoresis</kwd></kwd-group><funding-group><award-group><funding-source country="United States">National Center for Research Resources : NCRR</funding-source><award-id>S10 RR023044-01 || RR</award-id></award-group></funding-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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