Metabolomic Profiling of the Nectars of Aquilegia pubescens and A. Canadensis
Identifieur interne : 000088 ( Pmc/Checkpoint ); précédent : 000087; suivant : 000089Metabolomic Profiling of the Nectars of Aquilegia pubescens and A. Canadensis
Auteurs : Christos Noutsos [États-Unis] ; Ann M. Perera [États-Unis] ; Basil J. Nikolau [États-Unis] ; Samuel M. D. Seaver [États-Unis] ; Doreen H. Ware [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2015.
Abstract
To date, variation in nectar chemistry of flowering plants has not been studied in detail. Such variation exerts considerable influence on pollinator–plant interactions, as well as on flower traits that play important roles in the selection of a plant for visitation by specific pollinators. Over the past 60 years the
Url:
DOI: 10.1371/journal.pone.0124501
PubMed: 25933103
PubMed Central: 4416886
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Metabolomic Profiling of the Nectars of <italic>Aquilegia pubescens</italic> and <italic>A</italic>. <italic>Canadensis</italic></title><author><name sortKey="Noutsos, Christos" sort="Noutsos, Christos" uniqKey="Noutsos C" first="Christos" last="Noutsos">Christos Noutsos</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Cold Spring Harbor Laboratory, Cold Spring Harbor, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Perera, Ann M" sort="Perera, Ann M" uniqKey="Perera A" first="Ann M." last="Perera">Ann M. Perera</name><affiliation wicri:level="4"><nlm:aff id="aff002"><addr-line>W.M. Keck Metabolomics Research Laboratory, Iowa State University, Ames, Iowa, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>W.M. Keck Metabolomics Research Laboratory, Iowa State University, Ames, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Ames (Iowa)</settlement></placeName><orgName type="university">Université d'État de l'Iowa</orgName></affiliation></author><author><name sortKey="Nikolau, Basil J" sort="Nikolau, Basil J" uniqKey="Nikolau B" first="Basil J." last="Nikolau">Basil J. Nikolau</name><affiliation wicri:level="4"><nlm:aff id="aff002"><addr-line>W.M. Keck Metabolomics Research Laboratory, Iowa State University, Ames, Iowa, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>W.M. Keck Metabolomics Research Laboratory, Iowa State University, Ames, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Ames (Iowa)</settlement></placeName><orgName type="university">Université d'État de l'Iowa</orgName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff003"><addr-line>Department of Biochemistry, Biophysics, and Molecular Biology, Iowa, State University, Ames, Iowa, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, Biophysics, and Molecular Biology, Iowa, State University, Ames, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region></placeName></affiliation></author><author><name sortKey="Seaver, Samuel M D" sort="Seaver, Samuel M D" uniqKey="Seaver S" first="Samuel M. D." last="Seaver">Samuel M. D. Seaver</name><affiliation wicri:level="2"><nlm:aff id="aff004"><addr-line>Mathematics and Computer Science Division, Argonne National Laboratory, Argonne, Illinois, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mathematics and Computer Science Division, Argonne National Laboratory, Argonne, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff005"><addr-line>Computation Institute, The University of Chicago, Chicago, Illinois, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Computation Institute, The University of Chicago, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Ware, Doreen H" sort="Ware, Doreen H" uniqKey="Ware D" first="Doreen H." last="Ware">Doreen H. Ware</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Cold Spring Harbor Laboratory, Cold Spring Harbor, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff006"><addr-line>USDA-ARS-NAA, Robert W. Holley Center, Ithaca, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA-ARS-NAA, Robert W. Holley Center, Ithaca, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25933103</idno><idno type="pmc">4416886</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4416886</idno><idno type="RBID">PMC:4416886</idno><idno type="doi">10.1371/journal.pone.0124501</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000075</idno><idno type="wicri:Area/Pmc/Curation">000075</idno><idno type="wicri:Area/Pmc/Checkpoint">000088</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Metabolomic Profiling of the Nectars of <italic>Aquilegia pubescens</italic> and <italic>A</italic>. <italic>Canadensis</italic></title><author><name sortKey="Noutsos, Christos" sort="Noutsos, Christos" uniqKey="Noutsos C" first="Christos" last="Noutsos">Christos Noutsos</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Cold Spring Harbor Laboratory, Cold Spring Harbor, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Perera, Ann M" sort="Perera, Ann M" uniqKey="Perera A" first="Ann M." last="Perera">Ann M. Perera</name><affiliation wicri:level="4"><nlm:aff id="aff002"><addr-line>W.M. Keck Metabolomics Research Laboratory, Iowa State University, Ames, Iowa, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>W.M. Keck Metabolomics Research Laboratory, Iowa State University, Ames, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Ames (Iowa)</settlement></placeName><orgName type="university">Université d'État de l'Iowa</orgName></affiliation></author><author><name sortKey="Nikolau, Basil J" sort="Nikolau, Basil J" uniqKey="Nikolau B" first="Basil J." last="Nikolau">Basil J. Nikolau</name><affiliation wicri:level="4"><nlm:aff id="aff002"><addr-line>W.M. Keck Metabolomics Research Laboratory, Iowa State University, Ames, Iowa, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>W.M. Keck Metabolomics Research Laboratory, Iowa State University, Ames, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Ames (Iowa)</settlement></placeName><orgName type="university">Université d'État de l'Iowa</orgName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff003"><addr-line>Department of Biochemistry, Biophysics, and Molecular Biology, Iowa, State University, Ames, Iowa, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, Biophysics, and Molecular Biology, Iowa, State University, Ames, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region></placeName></affiliation></author><author><name sortKey="Seaver, Samuel M D" sort="Seaver, Samuel M D" uniqKey="Seaver S" first="Samuel M. D." last="Seaver">Samuel M. D. Seaver</name><affiliation wicri:level="2"><nlm:aff id="aff004"><addr-line>Mathematics and Computer Science Division, Argonne National Laboratory, Argonne, Illinois, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mathematics and Computer Science Division, Argonne National Laboratory, Argonne, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff005"><addr-line>Computation Institute, The University of Chicago, Chicago, Illinois, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Computation Institute, The University of Chicago, Chicago, Illinois</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Ware, Doreen H" sort="Ware, Doreen H" uniqKey="Ware D" first="Doreen H." last="Ware">Doreen H. Ware</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Cold Spring Harbor Laboratory, Cold Spring Harbor, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff006"><addr-line>USDA-ARS-NAA, Robert W. Holley Center, Ithaca, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA-ARS-NAA, Robert W. Holley Center, Ithaca, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>To date, variation in nectar chemistry of flowering plants has not been studied in detail. Such variation exerts considerable influence on pollinator–plant interactions, as well as on flower traits that play important roles in the selection of a plant for visitation by specific pollinators. Over the past 60 years the <italic>Aquilegia</italic> genus has been used as a key model for speciation studies. In this study, we defined the metabolomic profiles of flower samples of two <italic>Aquilegia</italic> species, <italic>A</italic>. <italic>Canadensis</italic> and <italic>A</italic>. <italic>pubescens</italic>. We identified a total of 75 metabolites that were classified into six main categories: organic acids, fatty acids, amino acids, esters, sugars, and unknowns. The mean abundances of 25 of these metabolites were significantly different between the two species, providing insights into interspecies variation in floral chemistry. Using the PlantSEED biochemistry database, we found that the majority of these metabolites are involved in biosynthetic pathways. Finally, we explored the annotated genome of <italic>A</italic>. <italic>coerulea</italic>, using the PlantSEED pipeline and reconstructed the metabolic network of <italic>Aquilegia</italic>. This network, which contains the metabolic pathways involved in generating the observed chemical variation, is now publicly available from the DOE Systems Biology Knowledge Base (KBase; <ext-link ext-link-type="uri" xlink:href="http://kbase.us">http://kbase.us</ext-link>).</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Fenster, Cb" uniqKey="Fenster C">CB Fenster</name></author><author><name sortKey="Cheely, G" uniqKey="Cheely G">G Cheely</name></author><author><name sortKey="Dudash, Mr" uniqKey="Dudash M">MR Dudash</name></author><author><name sortKey="Reynolds, Rj" uniqKey="Reynolds R">RJ Reynolds</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baker, Hg Bi" uniqKey="Baker H">HG BI Baker</name></author><author><name sortKey="Jones, Ce Lr" uniqKey="Jones C">CE LR Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bernardello, G" uniqKey="Bernardello G">G Bernardello</name></author><author><name sortKey="Galetto, L" uniqKey="Galetto L">L Galetto</name></author><author><name sortKey="Forcone, A" uniqKey="Forcone A">A Forcone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jurgens, A" uniqKey="Jurgens A">A Jurgens</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dupont, Yl" uniqKey="Dupont Y">YL Dupont</name></author><author><name sortKey="Hansen, Dm" uniqKey="Hansen D">DM Hansen</name></author><author><name sortKey="Rasmussen, Jt" uniqKey="Rasmussen J">JT Rasmussen</name></author><author><name sortKey="Olesen, Jm" uniqKey="Olesen J">JM Olesen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roldan Serrano, As" uniqKey="Roldan Serrano A">AS Roldán-Serrano</name></author><author><name sortKey="Guerra Sanz, J" uniqKey="Guerra Sanz J">J Guerra-Sanz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wit, T" uniqKey="Wit T">T Wit</name></author><author><name sortKey="Jurgens, A" uniqKey="Jurgens A">A Jürgens</name></author><author><name sortKey="Geyer, R" uniqKey="Geyer R">R Geyer</name></author><author><name sortKey="Gottsberger, G" uniqKey="Gottsberger G">G Gottsberger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grant, V" uniqKey="Grant V">V Grant</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Whittall, Jb" uniqKey="Whittall J">JB Whittall</name></author><author><name sortKey="Hodges, Sa" uniqKey="Hodges S">SA Hodges</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hodges, Sa" uniqKey="Hodges S">SA Hodges</name></author><author><name sortKey="Arnold, Ml" uniqKey="Arnold M">ML Arnold</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Payson, Eb" uniqKey="Payson E">EB Payson</name></author><author><name sortKey="Museum, Siusn" uniqKey="Museum S">SiUsn museum</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baldwin, Mw" uniqKey="Baldwin M">MW Baldwin</name></author><author><name sortKey="Toda, Y" uniqKey="Toda Y">Y Toda</name></author><author><name sortKey="Nakagita, T" uniqKey="Nakagita T">T Nakagita</name></author><author><name sortKey="O Connell, Mj" uniqKey="O Connell M">MJ O'Connell</name></author><author><name sortKey="Klasing, Kc" uniqKey="Klasing K">KC Klasing</name></author><author><name sortKey="Misaka, T" uniqKey="Misaka T">T Misaka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Canto, A" uniqKey="Canto A">A Canto</name></author><author><name sortKey="Perez, R" uniqKey="Perez R">R Perez</name></author><author><name sortKey="Medrano, M" uniqKey="Medrano M">M Medrano</name></author><author><name sortKey="Castellanos, Mc" uniqKey="Castellanos M">MC Castellanos</name></author><author><name sortKey="Herrera, Cm" uniqKey="Herrera C">CM Herrera</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sumner, Lw" uniqKey="Sumner L">LW Sumner</name></author><author><name sortKey="Amberg, A" uniqKey="Amberg A">A Amberg</name></author><author><name sortKey="Barrett, D" uniqKey="Barrett D">D Barrett</name></author><author><name sortKey="Beale, Mh" uniqKey="Beale M">MH Beale</name></author><author><name sortKey="Beger, R" uniqKey="Beger R">R Beger</name></author><author><name sortKey="Daykin, Ca" uniqKey="Daykin C">CA Daykin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schmidt, Ma" uniqKey="Schmidt M">MA Schmidt</name></author><author><name sortKey="Barbazuk, Wb" uniqKey="Barbazuk W">WB Barbazuk</name></author><author><name sortKey="Sandford, M" uniqKey="Sandford M">M Sandford</name></author><author><name sortKey="May, G" uniqKey="May G">G May</name></author><author><name sortKey="Song, Zh" uniqKey="Song Z">ZH Song</name></author><author><name sortKey="Zhou, Wx" uniqKey="Zhou W">WX Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Quanbeck, Sm" uniqKey="Quanbeck S">SM Quanbeck</name></author><author><name sortKey="Brachova, L" uniqKey="Brachova L">L Brachova</name></author><author><name sortKey="Campbell, Aa" uniqKey="Campbell A">AA Campbell</name></author><author><name sortKey="Guan, X" uniqKey="Guan X">X Guan</name></author><author><name sortKey="Perera, A" uniqKey="Perera A">A Perera</name></author><author><name sortKey="He, K" uniqKey="He K">K He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goff, Sa" uniqKey="Goff S">SA Goff</name></author><author><name sortKey="Vaughn, M" uniqKey="Vaughn M">M Vaughn</name></author><author><name sortKey="Mckay, S" uniqKey="Mckay S">S McKay</name></author><author><name sortKey="Lyons, E" uniqKey="Lyons E">E Lyons</name></author><author><name sortKey="Stapleton, Ae" uniqKey="Stapleton A">AE Stapleton</name></author><author><name sortKey="Gessler, D" uniqKey="Gessler D">D Gessler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Seaver, Smd" uniqKey="Seaver S">SMD Seaver</name></author><author><name sortKey="Gerdes, S" uniqKey="Gerdes S">S Gerdes</name></author><author><name sortKey="Frelin, O" uniqKey="Frelin O">O Frelin</name></author><author><name sortKey="Lerma Ortiz, C" uniqKey="Lerma Ortiz C">C Lerma-Ortiz</name></author><author><name sortKey="Bradbury, Lmt" uniqKey="Bradbury L">LMT Bradbury</name></author><author><name sortKey="Zallot, R" uniqKey="Zallot R">R Zallot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Overbeek, R" uniqKey="Overbeek R">R Overbeek</name></author><author><name sortKey="Olson, R" uniqKey="Olson R">R Olson</name></author><author><name sortKey="Pusch, Gd" uniqKey="Pusch G">GD Pusch</name></author><author><name sortKey="Olsen, Gj" uniqKey="Olsen G">GJ Olsen</name></author><author><name sortKey="Davis, Jj" uniqKey="Davis J">JJ Davis</name></author><author><name sortKey="Disz, T" uniqKey="Disz T">T Disz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaczorowski, Rl" uniqKey="Kaczorowski R">RL Kaczorowski</name></author><author><name sortKey="Gardener, Mc" uniqKey="Gardener M">MC Gardener</name></author><author><name sortKey="Holtsford, Tp" uniqKey="Holtsford T">TP Holtsford</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wolff, D" uniqKey="Wolff D">D Wolff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gottsberger, G" uniqKey="Gottsberger G">G Gottsberger</name></author><author><name sortKey="Schrauwen, J" uniqKey="Schrauwen J">J Schrauwen</name></author><author><name sortKey="Linskens, Hf" uniqKey="Linskens H">HF Linskens</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fenster, Cb" uniqKey="Fenster C">CB Fenster</name></author><author><name sortKey="Armbruster, Ws" uniqKey="Armbruster W">WS Armbruster</name></author><author><name sortKey="Wilson, P" uniqKey="Wilson P">P Wilson</name></author><author><name sortKey="Dudash, Mr" uniqKey="Dudash M">MR Dudash</name></author><author><name sortKey="Thomson, Jd" uniqKey="Thomson J">JD Thomson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Burke, Jm" uniqKey="Burke J">JM Burke</name></author><author><name sortKey="Wyatt, R" uniqKey="Wyatt R">R Wyatt</name></author><author><name sortKey="Depamphilis, Cw" uniqKey="Depamphilis C">CW dePamphilis</name></author><author><name sortKey="Arnold, Ml" uniqKey="Arnold M">ML Arnold</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pyke, Gh" uniqKey="Pyke G">GH Pyke</name></author><author><name sortKey="Waser, Nm" uniqKey="Waser N">NM Waser</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zajacz, E" uniqKey="Zajacz E">E Zajacz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bieleski, Rl" uniqKey="Bieleski R">RL Bieleski</name></author><author><name sortKey="Redgwell, Rj" uniqKey="Redgwell R">RJ Redgwell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Payan, F" uniqKey="Payan F">F Payan</name></author><author><name sortKey="Leone, P" uniqKey="Leone P">P Leone</name></author><author><name sortKey="Porciero, S" uniqKey="Porciero S">S Porciero</name></author><author><name sortKey="Furniss, C" uniqKey="Furniss C">C Furniss</name></author><author><name sortKey="Tahir, T" uniqKey="Tahir T">T Tahir</name></author><author><name sortKey="Williamson, G" uniqKey="Williamson G">G Williamson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bender, R" uniqKey="Bender R">R Bender</name></author><author><name sortKey="Klinkenberg, P" uniqKey="Klinkenberg P">P Klinkenberg</name></author><author><name sortKey="Jiang, Zh" uniqKey="Jiang Z">ZH Jiang</name></author><author><name sortKey="Bauer, B" uniqKey="Bauer B">B Bauer</name></author><author><name sortKey="Karypis, G" uniqKey="Karypis G">G Karypis</name></author><author><name sortKey="Nguyen, N" uniqKey="Nguyen N">N Nguyen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gijbels, P" uniqKey="Gijbels P">P Gijbels</name></author><author><name sortKey="Van Den Ende, W" uniqKey="Van Den Ende W">W Van den Ende</name></author><author><name sortKey="Honnay, O" uniqKey="Honnay O">O Honnay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nepi, M" uniqKey="Nepi M">M Nepi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kirk, H" uniqKey="Kirk H">H Kirk</name></author><author><name sortKey="Cheng, Dd" uniqKey="Cheng D">DD Cheng</name></author><author><name sortKey="Choi, Yh" uniqKey="Choi Y">YH Choi</name></author><author><name sortKey="Vrieling, K" uniqKey="Vrieling K">K Vrieling</name></author><author><name sortKey="Klinkhamer, Pgl" uniqKey="Klinkhamer P">PGL Klinkhamer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baker, Hg" uniqKey="Baker H">HG Baker</name></author><author><name sortKey="Baker, I" uniqKey="Baker I">I Baker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baker, I" uniqKey="Baker I">I Baker</name></author><author><name sortKey="Baker, H G" uniqKey="Baker H">H.G. Baker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brice, At" uniqKey="Brice A">AT Brice</name></author><author><name sortKey="Grau, Cr" uniqKey="Grau C">CR Grau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bertazzini, M" uniqKey="Bertazzini M">M Bertazzini</name></author><author><name sortKey="Medrzycki, P" uniqKey="Medrzycki P">P Medrzycki</name></author><author><name sortKey="Bortolotti, L" uniqKey="Bortolotti L">L Bortolotti</name></author><author><name sortKey="Maistrello, L" uniqKey="Maistrello L">L Maistrello</name></author><author><name sortKey="Forlani, G" uniqKey="Forlani G">G Forlani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mato, I" uniqKey="Mato I">I Mato</name></author><author><name sortKey="Huidobro, J" uniqKey="Huidobro J">J. Huidobro</name></author><author><name sortKey="Simal Lozano, J" uniqKey="Simal Lozano J">J. Simal-Lozano</name></author><author><name sortKey="Sancho, M T" uniqKey="Sancho M">M. T. Sancho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shalit, M" uniqKey="Shalit M">M Shalit</name></author><author><name sortKey="Guterman, I" uniqKey="Guterman I">I Guterman</name></author><author><name sortKey="Volpin, H" uniqKey="Volpin H">H Volpin</name></author><author><name sortKey="Bar, E" uniqKey="Bar E">E Bar</name></author><author><name sortKey="Tamari, T" uniqKey="Tamari T">T Tamari</name></author><author><name sortKey="Menda, N" uniqKey="Menda N">N Menda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hodges, Sa" uniqKey="Hodges S">SA Hodges</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wyatt, R" uniqKey="Wyatt R">R Wyatt</name></author><author><name sortKey="Broyles, Sb" uniqKey="Broyles S">SB Broyles</name></author><author><name sortKey="Derda, Gs" uniqKey="Derda G">GS Derda</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25933103</article-id><article-id pub-id-type="pmc">4416886</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0124501</article-id><article-id pub-id-type="publisher-id">PONE-D-14-46474</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Metabolomic Profiling of the Nectars of <italic>Aquilegia pubescens</italic> and <italic>A</italic>. <italic>Canadensis</italic></article-title><alt-title alt-title-type="running-head">Metabolomics Profile Plants</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Noutsos</surname><given-names>Christos</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref rid="cor001" ref-type="corresp">*</xref></contrib><contrib contrib-type="author"><name><surname>Perera</surname><given-names>Ann M.</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nikolau</surname><given-names>Basil J.</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Seaver</surname><given-names>Samuel M. D.</given-names></name><xref ref-type="aff" rid="aff004"><sup>4</sup></xref><xref ref-type="aff" rid="aff005"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ware</surname><given-names>Doreen H.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff006"><sup>6</sup></xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America</addr-line></aff><aff id="aff002"><label>2</label><addr-line>W.M. Keck Metabolomics Research Laboratory, Iowa State University, Ames, Iowa, United States of America</addr-line></aff><aff id="aff003"><label>3</label><addr-line>Department of Biochemistry, Biophysics, and Molecular Biology, Iowa, State University, Ames, Iowa, United States of America</addr-line></aff><aff id="aff004"><label>4</label><addr-line>Mathematics and Computer Science Division, Argonne National Laboratory, Argonne, Illinois, United States of America</addr-line></aff><aff id="aff005"><label>5</label><addr-line>Computation Institute, The University of Chicago, Chicago, Illinois, United States of America</addr-line></aff><aff id="aff006"><label>6</label><addr-line>USDA-ARS-NAA, Robert W. Holley Center, Ithaca, New York, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Motta</surname><given-names>Andrea</given-names></name><role>Academic Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>National Research Council of Italy, ITALY</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: CN BJN SMDS. Performed the experiments: CN MAP SMDS. Analyzed the data: CN SMDS. Contributed reagents/materials/analysis tools: CN BJN MAP SMDS DW. Wrote the paper: CN SMDS BJN.</p></fn><corresp id="cor001">* E-mail: <email>cnoutsos@cshl.edu</email></corresp></author-notes><pub-date pub-type="epub"><day>1</day><month>5</month><year>2015</year></pub-date><pub-date pub-type="collection"><year>2015</year></pub-date><volume>10</volume><issue>5</issue><elocation-id>e0124501</elocation-id><history><date date-type="received"><day>21</day><month>10</month><year>2014</year></date><date date-type="accepted"><day>2</day><month>3</month><year>2015</year></date></history><permissions><license xlink:href="https://creativecommons.org/publicdomain/zero/1.0/"><license-p>This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/publicdomain/zero/1.0/">Creative Commons CC0</ext-link> public domain dedication</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="pone.0124501.pdf"></self-uri><abstract><p>To date, variation in nectar chemistry of flowering plants has not been studied in detail. Such variation exerts considerable influence on pollinator–plant interactions, as well as on flower traits that play important roles in the selection of a plant for visitation by specific pollinators. Over the past 60 years the <italic>Aquilegia</italic> genus has been used as a key model for speciation studies. In this study, we defined the metabolomic profiles of flower samples of two <italic>Aquilegia</italic> species, <italic>A</italic>. <italic>Canadensis</italic> and <italic>A</italic>. <italic>pubescens</italic>. We identified a total of 75 metabolites that were classified into six main categories: organic acids, fatty acids, amino acids, esters, sugars, and unknowns. The mean abundances of 25 of these metabolites were significantly different between the two species, providing insights into interspecies variation in floral chemistry. Using the PlantSEED biochemistry database, we found that the majority of these metabolites are involved in biosynthetic pathways. Finally, we explored the annotated genome of <italic>A</italic>. <italic>coerulea</italic>, using the PlantSEED pipeline and reconstructed the metabolic network of <italic>Aquilegia</italic>. This network, which contains the metabolic pathways involved in generating the observed chemical variation, is now publicly available from the DOE Systems Biology Knowledge Base (KBase; <ext-link ext-link-type="uri" xlink:href="http://kbase.us">http://kbase.us</ext-link>).</p></abstract><funding-group><funding-statement>This work was funded by grants from the National Science Foundation of United States of America under the Division of Biological Infrastructure to Dr. Doreen Ware who is Co-Principal Investigator to the DBI-1265382. Financial support was provided by the Department of Energy (DE-AC02-98CH10886) for Seaver SMD and to Christos Noutsos from the NSF by the BDI-1265383, the iPlant Collaborative Project. Basil J. Nikolau and and Ann M. Perera received no specific funding for this work. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><fig-count count="4"></fig-count><table-count count="3"></table-count><page-count count="13"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper and its Supporting Information files and from the <ext-link ext-link-type="uri" xlink:href="http://narrative.kbase.us/functional-site/#/ws/objects/Acoerulea">http://narrative.kbase.us/functional-site/#/ws/objects/Acoerulea</ext-link> site.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper and its Supporting Information files and from the <ext-link ext-link-type="uri" xlink:href="http://narrative.kbase.us/functional-site/#/ws/objects/Acoerulea">http://narrative.kbase.us/functional-site/#/ws/objects/Acoerulea</ext-link> site.</p></notes></front></pmc><affiliations><list><country><li>États-Unis</li></country><region><li>Illinois</li><li>Iowa</li><li>État de New York</li></region><settlement><li>Ames (Iowa)</li></settlement><orgName><li>Université d'État de l'Iowa</li></orgName></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Noutsos, Christos" sort="Noutsos, Christos" uniqKey="Noutsos C" first="Christos" last="Noutsos">Christos Noutsos</name></region><name sortKey="Nikolau, Basil J" sort="Nikolau, Basil J" uniqKey="Nikolau B" first="Basil J." last="Nikolau">Basil J. Nikolau</name><name sortKey="Nikolau, Basil J" sort="Nikolau, Basil J" uniqKey="Nikolau B" first="Basil J." last="Nikolau">Basil J. Nikolau</name><name sortKey="Perera, Ann M" sort="Perera, Ann M" uniqKey="Perera A" first="Ann M." last="Perera">Ann M. Perera</name><name sortKey="Seaver, Samuel M D" sort="Seaver, Samuel M D" uniqKey="Seaver S" first="Samuel M. D." last="Seaver">Samuel M. D. Seaver</name><name sortKey="Seaver, Samuel M D" sort="Seaver, Samuel M D" uniqKey="Seaver S" first="Samuel M. D." last="Seaver">Samuel M. D. Seaver</name><name sortKey="Ware, Doreen H" sort="Ware, Doreen H" uniqKey="Ware D" first="Doreen H." last="Ware">Doreen H. Ware</name><name sortKey="Ware, Doreen H" sort="Ware, Doreen H" uniqKey="Ware D" first="Doreen H." last="Ware">Doreen H. Ware</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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