Transcriptional activity of the giant barrel sponge, Xestospongia muta Holobiont: molecular evidence for metabolic interchange
Identifieur interne : 000054 ( Pmc/Checkpoint ); précédent : 000053; suivant : 000055Transcriptional activity of the giant barrel sponge, Xestospongia muta Holobiont: molecular evidence for metabolic interchange
Auteurs : Cara L. Fiore [États-Unis] ; Micheline Labrie [États-Unis] ; Jessica K. Jarett [États-Unis] ; Michael P. Lesser [États-Unis]Source :
- Frontiers in Microbiology [ 1664-302X ] ; 2015.
Abstract
Compared to our understanding of the taxonomic composition of the symbiotic microbes in marine sponges, the functional diversity of these symbionts is largely unknown. Furthermore, the application of genomic, transcriptomic, and proteomic techniques to functional questions on sponge host-symbiont interactions is in its infancy. In this study, we generated a transcriptome for the host and a metatranscriptome of its microbial symbionts for the giant barrel sponge,
Url:
DOI: 10.3389/fmicb.2015.00364
PubMed: 25972851
PubMed Central: 4412061
Affiliations:
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Transcriptional activity of the giant barrel sponge, <italic>Xestospongia muta</italic>
Holobiont: molecular evidence for metabolic interchange</title>
<author><name sortKey="Fiore, Cara L" sort="Fiore, Cara L" uniqKey="Fiore C" first="Cara L." last="Fiore">Cara L. Fiore</name>
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<author><name sortKey="Lesser, Michael P" sort="Lesser, Michael P" uniqKey="Lesser M" first="Michael P." last="Lesser">Michael P. Lesser</name>
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<series><title level="j">Frontiers in Microbiology</title>
<idno type="eISSN">1664-302X</idno>
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<front><div type="abstract" xml:lang="en"><p>Compared to our understanding of the taxonomic composition of the symbiotic microbes in marine sponges, the functional diversity of these symbionts is largely unknown. Furthermore, the application of genomic, transcriptomic, and proteomic techniques to functional questions on sponge host-symbiont interactions is in its infancy. In this study, we generated a transcriptome for the host and a metatranscriptome of its microbial symbionts for the giant barrel sponge, <italic>Xestospongia muta</italic>
, from the Caribbean. In combination with a gene-specific approach, our goals were to (1) characterize genetic evidence for nitrogen cycling in <italic>X. muta</italic>
, an important limiting nutrient on coral reefs (2) identify which prokaryotic symbiont lineages are metabolically active and, (3) characterize the metabolic potential of the prokaryotic community. <italic>Xestospongia muta</italic>
expresses genes from multiple nitrogen transformation pathways that when combined with the abundance of this sponge, and previous data on dissolved inorganic nitrogen fluxes, shows that this sponge is an important contributor to nitrogen cycling biogeochemistry on coral reefs. Additionally, we observed significant differences in gene expression of the archaeal <italic>amo</italic>
A gene, which is involved in ammonia oxidation, between coral reef locations consistent with differences in the fluxes of dissolved inorganic nitrogen previously reported. In regards to symbiont metabolic potential, the genes in the biosynthetic pathways of several amino acids were present in the prokaryotic metatranscriptome dataset but in the host-derived transcripts only the catabolic reactions for these amino acids were present. A similar pattern was observed for the B vitamins (riboflavin, biotin, thiamin, cobalamin). These results expand our understanding of biogeochemical cycling in sponges, and the metabolic interchange highlighted here advances the field of symbiont physiology by elucidating specific metabolic pathways where there is high potential for host-prokaryote interactions.</p>
</div>
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<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Antal, T K" uniqKey="Antal T">T. K. Antal</name>
</author>
<author><name sortKey="Lindblad, P" uniqKey="Lindblad P">P. Lindblad</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Arillo, A" uniqKey="Arillo A">A. Arillo</name>
</author>
<author><name sortKey="Bavestrello, G" uniqKey="Bavestrello G">G. Bavestrello</name>
</author>
<author><name sortKey="Burlando, B" uniqKey="Burlando B">B. Burlando</name>
</author>
<author><name sortKey="Sara, M" uniqKey="Sara M">M. Sarà</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bagwell, C E" uniqKey="Bagwell C">C. E. Bagwell</name>
</author>
<author><name sortKey="Piskorska, M" uniqKey="Piskorska M">M. Piskorska</name>
</author>
<author><name sortKey="Soule, T" uniqKey="Soule T">T. Soule</name>
</author>
<author><name sortKey="Petelos, A" uniqKey="Petelos A">A. Petelos</name>
</author>
<author><name sortKey="Yeager, C M" uniqKey="Yeager C">C. M. Yeager</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Boyer, J N" uniqKey="Boyer J">J. N. Boyer</name>
</author>
<author><name sortKey="Dailey, S K" uniqKey="Dailey S">S. K. Dailey</name>
</author>
<author><name sortKey="Gibson, P J" uniqKey="Gibson P">P. J. Gibson</name>
</author>
<author><name sortKey="Rogers, M T" uniqKey="Rogers M">M. T. Rogers</name>
</author>
<author><name sortKey="Mir Gonzalez, D" uniqKey="Mir Gonzalez D">D. Mir-Gonzalez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Braker, G" uniqKey="Braker G">G. Braker</name>
</author>
<author><name sortKey="Fesefeldt, A" uniqKey="Fesefeldt A">A. Fesefeldt</name>
</author>
<author><name sortKey="Witzel, K P" uniqKey="Witzel K">K.-P. Witzel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Braker, G" uniqKey="Braker G">G. Braker</name>
</author>
<author><name sortKey="Tiedje, J M" uniqKey="Tiedje J">J. M. Tiedje</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Calvo, L" uniqKey="Calvo L">L. Calvo</name>
</author>
<author><name sortKey="Cortey, M" uniqKey="Cortey M">M. Cortey</name>
</author>
<author><name sortKey="Garcia Marin, J L" uniqKey="Garcia Marin J">J.-L. García-Marín</name>
</author>
<author><name sortKey="Garcia Gil, L J" uniqKey="Garcia Gil L">L. J. Garcia-Gil</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, T W" uniqKey="Chen T">T.-W. Chen</name>
</author>
<author><name sortKey="Gan, R C R" uniqKey="Gan R">R.-C. R. Gan</name>
</author>
<author><name sortKey="Wu, T H" uniqKey="Wu T">T. H. Wu</name>
</author>
<author><name sortKey="Huang, P J" uniqKey="Huang P">P.-J. Huang</name>
</author>
<author><name sortKey="Lee, C Y" uniqKey="Lee C">C.-Y. Lee</name>
</author>
<author><name sortKey="Chen, Y Y M" uniqKey="Chen Y">Y.-Y. M. Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chien, Y T" uniqKey="Chien Y">Y.-T. Chien</name>
</author>
<author><name sortKey="Zinder, S H" uniqKey="Zinder S">S. H. Zinder</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chistoserdova, L" uniqKey="Chistoserdova L">L. Chistoserdova</name>
</author>
<author><name sortKey="Kalyuzhnaya, M G" uniqKey="Kalyuzhnaya M">M. G. Kalyuzhnaya</name>
</author>
<author><name sortKey="Lidstrom, M E" uniqKey="Lidstrom M">M. E. Lidstrom</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Conaco, C" uniqKey="Conaco C">C. Conaco</name>
</author>
<author><name sortKey="Neveu, P" uniqKey="Neveu P">P. Neveu</name>
</author>
<author><name sortKey="Zhou, H" uniqKey="Zhou H">H. Zhou</name>
</author>
<author><name sortKey="Arcila, M L" uniqKey="Arcila M">M. L. Arcila</name>
</author>
<author><name sortKey="Degnan, S M" uniqKey="Degnan S">S. M. Degnan</name>
</author>
<author><name sortKey="Degnan, B M" uniqKey="Degnan B">B. M. Degnan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Goeij, J M" uniqKey="De Goeij J">J. M. de Goeij</name>
</author>
<author><name sortKey="Van Oevelen, D" uniqKey="Van Oevelen D">D. van Oevelen</name>
</author>
<author><name sortKey="Vermeij, M J A" uniqKey="Vermeij M">M. J. A. Vermeij</name>
</author>
<author><name sortKey="Osinga, R" uniqKey="Osinga R">R. Osinga</name>
</author>
<author><name sortKey="Middelburg, J J" uniqKey="Middelburg J">J. J. Middelburg</name>
</author>
<author><name sortKey="De Goeij, A F P M" uniqKey="De Goeij A">A. F. P. M. de Goeij</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="D Elia, C F" uniqKey="D Elia C">C. F. D'Elia</name>
</author>
<author><name sortKey="Wiebe, W J" uniqKey="Wiebe W">W. J. Wiebe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Wit, P" uniqKey="De Wit P">P. De Wit</name>
</author>
<author><name sortKey="Pespeni, M H" uniqKey="Pespeni M">M. H. Pespeni</name>
</author>
<author><name sortKey="Ladner, J T" uniqKey="Ladner J">J. T. Ladner</name>
</author>
<author><name sortKey="Barshis, D J" uniqKey="Barshis D">D. J. Barshis</name>
</author>
<author><name sortKey="Seneca, F" uniqKey="Seneca F">F. Seneca</name>
</author>
<author><name sortKey="Jaris, H" uniqKey="Jaris H">H. Jaris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Diaz, C M" uniqKey="Diaz C">C. M. Diaz</name>
</author>
<author><name sortKey="Rutzler, K" uniqKey="Rutzler K">K. Rutzler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Diaz, M" uniqKey="Diaz M">M. Diaz</name>
</author>
<author><name sortKey="Ward, B" uniqKey="Ward B">B. Ward</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dillon, R J" uniqKey="Dillon R">R. J. Dillon</name>
</author>
<author><name sortKey="Dillon, V M" uniqKey="Dillon V">V. M. Dillon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Doxey, A C" uniqKey="Doxey A">A. C. Doxey</name>
</author>
<author><name sortKey="Kurtz, D A" uniqKey="Kurtz D">D. A. Kurtz</name>
</author>
<author><name sortKey="Lynch, M D J" uniqKey="Lynch M">M. D. J. Lynch</name>
</author>
<author><name sortKey="Sauder, L A" uniqKey="Sauder L">L. A. Sauder</name>
</author>
<author><name sortKey="Neufeld, J D" uniqKey="Neufeld J">J. D. Neufeld</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Erwin, P M" uniqKey="Erwin P">P. M. Erwin</name>
</author>
<author><name sortKey="Thacker, R W" uniqKey="Thacker R">R. W. Thacker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Erwin, P M" uniqKey="Erwin P">P. M. Erwin</name>
</author>
<author><name sortKey="Thacker, R W" uniqKey="Thacker R">R. W. Thacker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fan, L" uniqKey="Fan L">L. Fan</name>
</author>
<author><name sortKey="Reynolds, D" uniqKey="Reynolds D">D. Reynolds</name>
</author>
<author><name sortKey="Liu, M" uniqKey="Liu M">M. Liu</name>
</author>
<author><name sortKey="Stark, M" uniqKey="Stark M">M. Stark</name>
</author>
<author><name sortKey="Kjelleberg, S" uniqKey="Kjelleberg S">S. Kjelleberg</name>
</author>
<author><name sortKey="Webster, N S" uniqKey="Webster N">N. S. Webster</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fiore, C L" uniqKey="Fiore C">C. L. Fiore</name>
</author>
<author><name sortKey="Baker, D M" uniqKey="Baker D">D. M. Baker</name>
</author>
<author><name sortKey="Lesser, M P" uniqKey="Lesser M">M. P. Lesser</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fiore, C L" uniqKey="Fiore C">C. L. Fiore</name>
</author>
<author><name sortKey="Jarett, J K" uniqKey="Jarett J">J. K. Jarett</name>
</author>
<author><name sortKey="Lesser, M P" uniqKey="Lesser M">M. P. Lesser</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fiore, C L" uniqKey="Fiore C">C. L. Fiore</name>
</author>
<author><name sortKey="Jarett, J K" uniqKey="Jarett J">J. K. Jarett</name>
</author>
<author><name sortKey="Olson, N D" uniqKey="Olson N">N. D. Olson</name>
</author>
<author><name sortKey="Lesser, M P" uniqKey="Lesser M">M. P. Lesser</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Francis, C A" uniqKey="Francis C">C. A. Francis</name>
</author>
<author><name sortKey="Roberts, K J" uniqKey="Roberts K">K. J. Roberts</name>
</author>
<author><name sortKey="Beman, J M" uniqKey="Beman J">J. M. Beman</name>
</author>
<author><name sortKey="Santoro, A E" uniqKey="Santoro A">A. E. Santoro</name>
</author>
<author><name sortKey="Oakley, B B" uniqKey="Oakley B">B. B. Oakley</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Freeman, C J" uniqKey="Freeman C">C. J. Freeman</name>
</author>
<author><name sortKey="Thacker, R W" uniqKey="Thacker R">R. W. Thacker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Frias Lopez, J" uniqKey="Frias Lopez J">J. Frias-Lopez</name>
</author>
<author><name sortKey="Shi, Y" uniqKey="Shi Y">Y. Shi</name>
</author>
<author><name sortKey="Tyson, G W" uniqKey="Tyson G">G. W. Tyson</name>
</author>
<author><name sortKey="Coleman, M L" uniqKey="Coleman M">M. L. Coleman</name>
</author>
<author><name sortKey="Schuster, S C" uniqKey="Schuster S">S. C. Schuster</name>
</author>
<author><name sortKey="Chrisholm, S W" uniqKey="Chrisholm S">S. W. Chrisholm</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grabherr, M G" uniqKey="Grabherr M">M. G. Grabherr</name>
</author>
<author><name sortKey="Haas, B J" uniqKey="Haas B">B. J. Haas</name>
</author>
<author><name sortKey="Yassour, M" uniqKey="Yassour M">M. Yassour</name>
</author>
<author><name sortKey="Levin, J Z" uniqKey="Levin J">J. Z. Levin</name>
</author>
<author><name sortKey="Thompson, D A" uniqKey="Thompson D">D. A. Thompson</name>
</author>
<author><name sortKey="Amit, I" uniqKey="Amit I">I. Amit</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hallam, S J" uniqKey="Hallam S">S. J. Hallam</name>
</author>
<author><name sortKey="Konstantinidis, K T" uniqKey="Konstantinidis K">K. T. Konstantinidis</name>
</author>
<author><name sortKey="Putnam, N" uniqKey="Putnam N">N. Putnam</name>
</author>
<author><name sortKey="Schleper, C" uniqKey="Schleper C">C. Schleper</name>
</author>
<author><name sortKey="Watanabe, Y I" uniqKey="Watanabe Y">Y.-I. Watanabe</name>
</author>
<author><name sortKey="Sugahara, J" uniqKey="Sugahara J">J. Sugahara</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hallam, S J" uniqKey="Hallam S">S. J. Hallam</name>
</author>
<author><name sortKey="Mincer, T J" uniqKey="Mincer T">T. J. Mincer</name>
</author>
<author><name sortKey="Schleper, C" uniqKey="Schleper C">C. Schleper</name>
</author>
<author><name sortKey="Preston, C M" uniqKey="Preston C">C. M. Preston</name>
</author>
<author><name sortKey="Roberts, K" uniqKey="Roberts K">K. Roberts</name>
</author>
<author><name sortKey="Richardson, P M" uniqKey="Richardson P">P. M. Richardson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hamada, M" uniqKey="Hamada M">M. Hamada</name>
</author>
<author><name sortKey="Shoguchin, E" uniqKey="Shoguchin E">E. Shoguchin</name>
</author>
<author><name sortKey="Shinzato, C" uniqKey="Shinzato C">C. Shinzato</name>
</author>
<author><name sortKey="Kawashima, T" uniqKey="Kawashima T">T. Kawashima</name>
</author>
<author><name sortKey="Miller, D J" uniqKey="Miller D">D. J. Miller</name>
</author>
<author><name sortKey="Satoh, N" uniqKey="Satoh N">N. Satoh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hentschel, U" uniqKey="Hentschel U">U. Hentschel</name>
</author>
<author><name sortKey="Fieseler, L" uniqKey="Fieseler L">L. Fieseler</name>
</author>
<author><name sortKey="Wehrl, M" uniqKey="Wehrl M">M. Wehrl</name>
</author>
<author><name sortKey="Gernert, C" uniqKey="Gernert C">C. Gernert</name>
</author>
<author><name sortKey="Steinert, M" uniqKey="Steinert M">M. Steinert</name>
</author>
<author><name sortKey="Horn, M" uniqKey="Horn M">M. Horn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hentschel, U" uniqKey="Hentschel U">U. Hentschel</name>
</author>
<author><name sortKey="Piel, J" uniqKey="Piel J">J. Piel</name>
</author>
<author><name sortKey="Degnan, S M" uniqKey="Degnan S">S. M. Degnan</name>
</author>
<author><name sortKey="Taylor, M W" uniqKey="Taylor M">M. W. Taylor</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hoffmann, F" uniqKey="Hoffmann F">F. Hoffmann</name>
</author>
<author><name sortKey="Radax, R" uniqKey="Radax R">R. Radax</name>
</author>
<author><name sortKey="Woebken, D" uniqKey="Woebken D">D. Woebken</name>
</author>
<author><name sortKey="Holtappels, M" uniqKey="Holtappels M">M. Holtappels</name>
</author>
<author><name sortKey="Lavik, G" uniqKey="Lavik G">G. Lavik</name>
</author>
<author><name sortKey="Rapp, H T" uniqKey="Rapp H">H. T. Rapp</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huson, D H" uniqKey="Huson D">D. H. Huson</name>
</author>
<author><name sortKey="Auch, A F" uniqKey="Auch A">A. F. Auch</name>
</author>
<author><name sortKey="Qi, J" uniqKey="Qi J">J. Qi</name>
</author>
<author><name sortKey="Schuster, S C" uniqKey="Schuster S">S. C. Schuster</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jarett, J" uniqKey="Jarett J">J. Jarett</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Junier, P" uniqKey="Junier P">P. Junier</name>
</author>
<author><name sortKey="Kim, O S" uniqKey="Kim O">O.-S. Kim</name>
</author>
<author><name sortKey="Hadas, O" uniqKey="Hadas O">O. Hadas</name>
</author>
<author><name sortKey="Imhoff, J F" uniqKey="Imhoff J">J. F. Imhoff</name>
</author>
<author><name sortKey="Witzel, K P" uniqKey="Witzel K">K.-P. Witzel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kamke, J" uniqKey="Kamke J">J. Kamke</name>
</author>
<author><name sortKey="Taylor, M W" uniqKey="Taylor M">M. W. Taylor</name>
</author>
<author><name sortKey="Schmitt, S" uniqKey="Schmitt S">S. Schmitt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kanehisa, M" uniqKey="Kanehisa M">M. Kanehisa</name>
</author>
<author><name sortKey="Goto, S" uniqKey="Goto S">S. Goto</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kanehisa, M" uniqKey="Kanehisa M">M. Kanehisa</name>
</author>
<author><name sortKey="Goto, S" uniqKey="Goto S">S. Goto</name>
</author>
<author><name sortKey="Sato, Y" uniqKey="Sato Y">Y. Sato</name>
</author>
<author><name sortKey="Furumichi, M" uniqKey="Furumichi M">M. Furumichi</name>
</author>
<author><name sortKey="Tanabe, M" uniqKey="Tanabe M">M. Tanabe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kennedy, J" uniqKey="Kennedy J">J. Kennedy</name>
</author>
<author><name sortKey="Flemer, B" uniqKey="Flemer B">B. Flemer</name>
</author>
<author><name sortKey="Jackson, S A" uniqKey="Jackson S">S. A. Jackson</name>
</author>
<author><name sortKey="Morrisssey, J P" uniqKey="Morrisssey J">J. P. Morrisssey</name>
</author>
<author><name sortKey="O Gara, F" uniqKey="O Gara F">F. O'Gara</name>
</author>
<author><name sortKey="Dobson, A D W" uniqKey="Dobson A">A. D. W. Dobson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Knapp, A" uniqKey="Knapp A">A. Knapp</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kvennefors, E C E" uniqKey="Kvennefors E">E. C. E. Kvennefors</name>
</author>
<author><name sortKey="Leggat, W" uniqKey="Leggat W">W. Leggat</name>
</author>
<author><name sortKey="Kerr, C C" uniqKey="Kerr C">C. C. Kerr</name>
</author>
<author><name sortKey="Ainsworth, T D" uniqKey="Ainsworth T">T. D. Ainsworth</name>
</author>
<author><name sortKey="Hoegh Guldberg, O" uniqKey="Hoegh Guldberg O">O. Hoegh-Guldberg</name>
</author>
<author><name sortKey="Barnes, A C" uniqKey="Barnes A">A. C. Barnes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, O O" uniqKey="Lee O">O. O. Lee</name>
</author>
<author><name sortKey="Wong, Y H" uniqKey="Wong Y">Y. H. Wong</name>
</author>
<author><name sortKey="Qian, P Y" uniqKey="Qian P">P. Y. Qian</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lesser, M P" uniqKey="Lesser M">M. P. Lesser</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lesser, M P" uniqKey="Lesser M">M. P. Lesser</name>
</author>
<author><name sortKey="Stat, M" uniqKey="Stat M">M. Stat</name>
</author>
<author><name sortKey="Gates, R D" uniqKey="Gates R">R. D. Gates</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, Z Y" uniqKey="Li Z">Z.-Y. Li</name>
</author>
<author><name sortKey="Wang, Y Z" uniqKey="Wang Y">Y.-Z. Wang</name>
</author>
<author><name sortKey="He, L M" uniqKey="He L">L.-M. He</name>
</author>
<author><name sortKey="Zheng, H J" uniqKey="Zheng H">H.-J. Zheng</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lopez Legentil, S" uniqKey="Lopez Legentil S">S. Lopez-Legentil</name>
</author>
<author><name sortKey="Erwin, P M" uniqKey="Erwin P">P. M. Erwin</name>
</author>
<author><name sortKey="Pawlik, J R" uniqKey="Pawlik J">J. R. Pawlik</name>
</author>
<author><name sortKey="Song, B" uniqKey="Song B">B. Song</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mccutcheon, J P" uniqKey="Mccutcheon J">J. P. McCutcheon</name>
</author>
<author><name sortKey="Moran, N A" uniqKey="Moran N">N. A. Moran</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mcmurray, S E" uniqKey="Mcmurray S">S. E. McMurray</name>
</author>
<author><name sortKey="Blum, J E" uniqKey="Blum J">J. E. Blum</name>
</author>
<author><name sortKey="Pawlik, J R" uniqKey="Pawlik J">J. R. Pawlik</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mcmurray, S E" uniqKey="Mcmurray S">S. E. McMurray</name>
</author>
<author><name sortKey="Henkel, T P" uniqKey="Henkel T">T. P. Henkel</name>
</author>
<author><name sortKey="Pawlik, J R" uniqKey="Pawlik J">J. R. Pawlik</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meyer, O" uniqKey="Meyer O">O. Meyer</name>
</author>
<author><name sortKey="Schlegel, H G" uniqKey="Schlegel H">H. G. Schlegel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Miller, C S" uniqKey="Miller C">C. S. Miller</name>
</author>
<author><name sortKey="Baker, B J" uniqKey="Baker B">B. J. Baker</name>
</author>
<author><name sortKey="Thomas, B C" uniqKey="Thomas B">B. C. Thomas</name>
</author>
<author><name sortKey="Singer, S W" uniqKey="Singer S">S. W. Singer</name>
</author>
<author><name sortKey="Banfield, J F" uniqKey="Banfield J">J. F. Banfield</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mohamed, N M" uniqKey="Mohamed N">N. M. Mohamed</name>
</author>
<author><name sortKey="Colman, A S" uniqKey="Colman A">A. S. Colman</name>
</author>
<author><name sortKey="Tai, Y" uniqKey="Tai Y">Y. Tai</name>
</author>
<author><name sortKey="Hill, R T" uniqKey="Hill R">R. T. Hill</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moitinho Silva, L" uniqKey="Moitinho Silva L">L. Moitinho-Silva</name>
</author>
<author><name sortKey="Seridi, L" uniqKey="Seridi L">L. Seridi</name>
</author>
<author><name sortKey="Ryu, T" uniqKey="Ryu T">T. Ryu</name>
</author>
<author><name sortKey="Voolstra, C R" uniqKey="Voolstra C">C. R. Voolstra</name>
</author>
<author><name sortKey="Ravasi, T" uniqKey="Ravasi T">T. Ravasi</name>
</author>
<author><name sortKey="Hentschel, U" uniqKey="Hentschel U">U. Hentschel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Montalvo, N F" uniqKey="Montalvo N">N. F. Montalvo</name>
</author>
<author><name sortKey="Davis, J" uniqKey="Davis J">J. Davis</name>
</author>
<author><name sortKey="Vicente, J" uniqKey="Vicente J">J. Vicente</name>
</author>
<author><name sortKey="Pittiglio, R" uniqKey="Pittiglio R">R. Pittiglio</name>
</author>
<author><name sortKey="Ravel, J" uniqKey="Ravel J">J. Ravel</name>
</author>
<author><name sortKey="Hill, R T" uniqKey="Hill R">R. T. Hill</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Montalvo, N F" uniqKey="Montalvo N">N. F. Montalvo</name>
</author>
<author><name sortKey="Hill, R T" uniqKey="Hill R">R. T. Hill</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Muscatine, L" uniqKey="Muscatine L">L. Muscatine</name>
</author>
<author><name sortKey="Porter, J W" uniqKey="Porter J">J. W. Porter</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nichols, S A" uniqKey="Nichols S">S. A. Nichols</name>
</author>
<author><name sortKey="Dirks, W" uniqKey="Dirks W">W. Dirks</name>
</author>
<author><name sortKey="Pearse, J S" uniqKey="Pearse J">J. S. Pearse</name>
</author>
<author><name sortKey="King, N" uniqKey="King N">N. King</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Noyer Weidner, M" uniqKey="Noyer Weidner M">M. Noyer-Weidner</name>
</author>
<author><name sortKey="Trautner, T A" uniqKey="Trautner T">T. A. Trautner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="O Brien, R G" uniqKey="O Brien R">R. G. O'Brien</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oelgeschl Ger, E" uniqKey="Oelgeschl Ger E">E. Oelgeschläger</name>
</author>
<author><name sortKey="Rother, M" uniqKey="Rother M">M. Rother</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ohkuma, M" uniqKey="Ohkuma M">M. Ohkuma</name>
</author>
<author><name sortKey="Kudo, T" uniqKey="Kudo T">T. Kudo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Olson, J B" uniqKey="Olson J">J. B. Olson</name>
</author>
<author><name sortKey="Steppe, T F" uniqKey="Steppe T">T. F. Steppe</name>
</author>
<author><name sortKey="Litaker, R W" uniqKey="Litaker R">R. W. Litaker</name>
</author>
<author><name sortKey="Paerl, H W" uniqKey="Paerl H">H. W. Paerl</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Olson, N D" uniqKey="Olson N">N. D. Olson</name>
</author>
<author><name sortKey="Ainsworth, T D" uniqKey="Ainsworth T">T. D. Ainsworth</name>
</author>
<author><name sortKey="Gates, R D" uniqKey="Gates R">R. D. Gates</name>
</author>
<author><name sortKey="Takabayashi, M" uniqKey="Takabayashi M">M. Takabayashi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Olson, N D" uniqKey="Olson N">N. D. Olson</name>
</author>
<author><name sortKey="Lesser, M P" uniqKey="Lesser M">M. P. Lesser</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Parsek, M R" uniqKey="Parsek M">M. R. Parsek</name>
</author>
<author><name sortKey="Greenberg, E P" uniqKey="Greenberg E">E. P. Greenberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Prasanna, R" uniqKey="Prasanna R">R. Prasanna</name>
</author>
<author><name sortKey="Kumar, V" uniqKey="Kumar V">V. Kumar</name>
</author>
<author><name sortKey="Kumar, S" uniqKey="Kumar S">S. Kumar</name>
</author>
<author><name sortKey="Yadav, A K" uniqKey="Yadav A">A. K. Yadav</name>
</author>
<author><name sortKey="Tripathi, U" uniqKey="Tripathi U">U. Tripathi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Quast, C" uniqKey="Quast C">C. Quast</name>
</author>
<author><name sortKey="Pruesse, E" uniqKey="Pruesse E">E. Pruesse</name>
</author>
<author><name sortKey="Yilmaz, P" uniqKey="Yilmaz P">P. Yilmaz</name>
</author>
<author><name sortKey="Gerken, J" uniqKey="Gerken J">J. Gerken</name>
</author>
<author><name sortKey="Schweer, T" uniqKey="Schweer T">T. Schweer</name>
</author>
<author><name sortKey="Yarza, P" uniqKey="Yarza P">P. Yarza</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Radax, R" uniqKey="Radax R">R. Radax</name>
</author>
<author><name sortKey="Rattei, T" uniqKey="Rattei T">T. Rattei</name>
</author>
<author><name sortKey="Lanzen, A" uniqKey="Lanzen A">A. Lanzen</name>
</author>
<author><name sortKey="Bayer, C" uniqKey="Bayer C">C. Bayer</name>
</author>
<author><name sortKey="Rapp, H T" uniqKey="Rapp H">H. T. Rapp</name>
</author>
<author><name sortKey="Urich, T" uniqKey="Urich T">T. Urich</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reiswig, H M" uniqKey="Reiswig H">H. M. Reiswig</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Riesgo, A" uniqKey="Riesgo A">A. Riesgo</name>
</author>
<author><name sortKey="Farrar, N" uniqKey="Farrar N">N. Farrar</name>
</author>
<author><name sortKey="Windsor, P J" uniqKey="Windsor P">P. J. Windsor</name>
</author>
<author><name sortKey="Giribet, G" uniqKey="Giribet G">G. Giribet</name>
</author>
<author><name sortKey="Leys, S P" uniqKey="Leys S">S. P. Leys</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roberts, K L" uniqKey="Roberts K">K. L. Roberts</name>
</author>
<author><name sortKey="Kessler, A J" uniqKey="Kessler A">A. J. Kessler</name>
</author>
<author><name sortKey="Grace, M R" uniqKey="Grace M">M. R. Grace</name>
</author>
<author><name sortKey="Cook, P L M" uniqKey="Cook P">P. L. M. Cook</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rotthauwe, J H" uniqKey="Rotthauwe J">J.-H. Rotthauwe</name>
</author>
<author><name sortKey="Witzel, K P" uniqKey="Witzel K">K.-P. Witzel</name>
</author>
<author><name sortKey="Liesack, W" uniqKey="Liesack W">W. Liesack</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Royston, P" uniqKey="Royston P">P. Royston</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sa Udo Wilhelmy, S A" uniqKey="Sa Udo Wilhelmy S">S. A. Sañudo-Wilhelmy</name>
</author>
<author><name sortKey="G Mez Consarnau, L" uniqKey="G Mez Consarnau L">L. Gómez-Consarnau</name>
</author>
<author><name sortKey="Suffridge, C" uniqKey="Suffridge C">C. Suffridge</name>
</author>
<author><name sortKey="Webb, E A" uniqKey="Webb E">E. A. Webb</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sayers, E W" uniqKey="Sayers E">E. W. Sayers</name>
</author>
<author><name sortKey="Barrett, T" uniqKey="Barrett T">T. Barrett</name>
</author>
<author><name sortKey="Benson, D A" uniqKey="Benson D">D. A. Benson</name>
</author>
<author><name sortKey="Bolton, E" uniqKey="Bolton E">E. Bolton</name>
</author>
<author><name sortKey="Bryant, S H" uniqKey="Bryant S">S. H. Bryant</name>
</author>
<author><name sortKey="Canese, K" uniqKey="Canese K">K. Canese</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schmid, M C" uniqKey="Schmid M">M. C. Schmid</name>
</author>
<author><name sortKey="Maas, M" uniqKey="Maas M">M. Maas</name>
</author>
<author><name sortKey="Dapena, A" uniqKey="Dapena A">A. Dapena</name>
</author>
<author><name sortKey="Van De Pas Schoonen, K" uniqKey="Van De Pas Schoonen K">K. van de Pas-Schoonen</name>
</author>
<author><name sortKey="Van De Vossenber, J" uniqKey="Van De Vossenber J">J. van de Vossenber</name>
</author>
<author><name sortKey="Kartal, B" uniqKey="Kartal B">B. Kartal</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schmieder, R" uniqKey="Schmieder R">R. Schmieder</name>
</author>
<author><name sortKey="Edwards, R" uniqKey="Edwards R">R. Edwards</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schmitt, S" uniqKey="Schmitt S">S. Schmitt</name>
</author>
<author><name sortKey="Tsai, P" uniqKey="Tsai P">P. Tsai</name>
</author>
<author><name sortKey="Bell, J" uniqKey="Bell J">J. Bell</name>
</author>
<author><name sortKey="Fromont, J" uniqKey="Fromont J">J. Fromont</name>
</author>
<author><name sortKey="Ilan, M" uniqKey="Ilan M">M. Ilan</name>
</author>
<author><name sortKey="Lindquist, N" uniqKey="Lindquist N">N. Lindquist</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Seutin, G" uniqKey="Seutin G">G. Seutin</name>
</author>
<author><name sortKey="White, B N" uniqKey="White B">B. N. White</name>
</author>
<author><name sortKey="Boag, P T" uniqKey="Boag P">P. T. Boag</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shu, Q" uniqKey="Shu Q">Q. Shu</name>
</author>
<author><name sortKey="Jiao, N" uniqKey="Jiao N">N. Jiao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Siegl, A" uniqKey="Siegl A">A. Siegl</name>
</author>
<author><name sortKey="Kamke, J" uniqKey="Kamke J">J. Kamke</name>
</author>
<author><name sortKey="Hochmuth, T" uniqKey="Hochmuth T">T. Hochmuth</name>
</author>
<author><name sortKey="Piel, J O R" uniqKey="Piel J">J. O. R. Piel</name>
</author>
<author><name sortKey="Richter, M" uniqKey="Richter M">M. Richter</name>
</author>
<author><name sortKey="Liang, C" uniqKey="Liang C">C. Liang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Simister, R L" uniqKey="Simister R">R. L. Simister</name>
</author>
<author><name sortKey="Deines, P" uniqKey="Deines P">P. Deines</name>
</author>
<author><name sortKey="Botte, E S" uniqKey="Botte E">E. S. Botté</name>
</author>
<author><name sortKey="Webster, N S" uniqKey="Webster N">N. S. Webster</name>
</author>
<author><name sortKey="Taylor, M W" uniqKey="Taylor M">M. W. Taylor</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Southwell, M W" uniqKey="Southwell M">M. W. Southwell</name>
</author>
<author><name sortKey="Weisz, J B" uniqKey="Weisz J">J. B. Weisz</name>
</author>
<author><name sortKey="Martens, C S" uniqKey="Martens C">C. S. Martens</name>
</author>
<author><name sortKey="Lindquist, N" uniqKey="Lindquist N">N. Lindquist</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Spaepen, S" uniqKey="Spaepen S">S. Spaepen</name>
</author>
<author><name sortKey="Vanderleyden, J" uniqKey="Vanderleyden J">J. Vanderleyden</name>
</author>
<author><name sortKey="Remans, R" uniqKey="Remans R">R. Remans</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Steger, D" uniqKey="Steger D">D. Steger</name>
</author>
<author><name sortKey="Ettinger Epstein, P" uniqKey="Ettinger Epstein P">P. Ettinger-Epstein</name>
</author>
<author><name sortKey="Whalan, S" uniqKey="Whalan S">S. Whalan</name>
</author>
<author><name sortKey="Hentschel, U" uniqKey="Hentschel U">U. Hentschel</name>
</author>
<author><name sortKey="De Nys, R" uniqKey="De Nys R">R. De Nys</name>
</author>
<author><name sortKey="Wagner, M" uniqKey="Wagner M">M. Wagner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Steindler, L" uniqKey="Steindler L">L. Steindler</name>
</author>
<author><name sortKey="Huchon, D" uniqKey="Huchon D">D. Huchon</name>
</author>
<author><name sortKey="Avni, A" uniqKey="Avni A">A. Avni</name>
</author>
<author><name sortKey="Ilan, M" uniqKey="Ilan M">M. Ilan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sun, S" uniqKey="Sun S">S. Sun</name>
</author>
<author><name sortKey="Chen, J" uniqKey="Chen J">J. Chen</name>
</author>
<author><name sortKey="Li, W" uniqKey="Li W">W. Li</name>
</author>
<author><name sortKey="Altintas, I" uniqKey="Altintas I">I. Altintas</name>
</author>
<author><name sortKey="Lin, A" uniqKey="Lin A">A. Lin</name>
</author>
<author><name sortKey="Peltier, S" uniqKey="Peltier S">S. Peltier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Taprab, Y" uniqKey="Taprab Y">Y. Taprab</name>
</author>
<author><name sortKey="Johjima, T" uniqKey="Johjima T">T. Johjima</name>
</author>
<author><name sortKey="Maeda, Y" uniqKey="Maeda Y">Y. Maeda</name>
</author>
<author><name sortKey="Moriya, S" uniqKey="Moriya S">S. Moriya</name>
</author>
<author><name sortKey="Trakulnaleamsai, S" uniqKey="Trakulnaleamsai S">S. Trakulnaleamsai</name>
</author>
<author><name sortKey="Noparatnaraporn, N" uniqKey="Noparatnaraporn N">N. Noparatnaraporn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Taylor, M W" uniqKey="Taylor M">M. W. Taylor</name>
</author>
<author><name sortKey="Radax, R" uniqKey="Radax R">R. Radax</name>
</author>
<author><name sortKey="Stegor, D" uniqKey="Stegor D">D. Stegor</name>
</author>
<author><name sortKey="Wagner, M" uniqKey="Wagner M">M. Wagner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thomas, T" uniqKey="Thomas T">T. Thomas</name>
</author>
<author><name sortKey="Rusch, D" uniqKey="Rusch D">D. Rusch</name>
</author>
<author><name sortKey="Demaere, M Z" uniqKey="Demaere M">M. Z. DeMaere</name>
</author>
<author><name sortKey="Yung, P Y" uniqKey="Yung P">P. Y. Yung</name>
</author>
<author><name sortKey="Lewis, M" uniqKey="Lewis M">M. Lewis</name>
</author>
<author><name sortKey="Halpern, A" uniqKey="Halpern A">A. Halpern</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ulrich, T" uniqKey="Ulrich T">T. Ulrich</name>
</author>
<author><name sortKey="Lanzen, A" uniqKey="Lanzen A">A. Lanzén</name>
</author>
<author><name sortKey="Qi, J" uniqKey="Qi J">J. Qi</name>
</author>
<author><name sortKey="Huson, D H" uniqKey="Huson D">D. H. Huson</name>
</author>
<author><name sortKey="Schleper, C" uniqKey="Schleper C">C. Schleper</name>
</author>
<author><name sortKey="Schuster, S C" uniqKey="Schuster S">S. C Schuster</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q. Wang</name>
</author>
<author><name sortKey="Garrity, G M" uniqKey="Garrity G">G. M. Garrity</name>
</author>
<author><name sortKey="Tiedje, J M" uniqKey="Tiedje J">J. M. Tiedje</name>
</author>
<author><name sortKey="Cole, J R" uniqKey="Cole J">J. R. Cole</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Webster, N S" uniqKey="Webster N">N. S. Webster</name>
</author>
<author><name sortKey="Taylor, M W" uniqKey="Taylor M">M. W. Taylor</name>
</author>
<author><name sortKey="Behnam, F" uniqKey="Behnam F">F. Behnam</name>
</author>
<author><name sortKey="Lucker, S" uniqKey="Lucker S">S. Lucker</name>
</author>
<author><name sortKey="Rattei, T" uniqKey="Rattei T">T. Rattei</name>
</author>
<author><name sortKey="Whalen, S" uniqKey="Whalen S">S. Whalen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Weisz, J B" uniqKey="Weisz J">J. B. Weisz</name>
</author>
<author><name sortKey="Lindquist, N" uniqKey="Lindquist N">N. Lindquist</name>
</author>
<author><name sortKey="Martens, C S" uniqKey="Martens C">C. S. Martens</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wilkinson, C" uniqKey="Wilkinson C">C. Wilkinson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wilkinson, C" uniqKey="Wilkinson C">C. Wilkinson</name>
</author>
<author><name sortKey="Fay, P" uniqKey="Fay P">P. Fay</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zehr, J P" uniqKey="Zehr J">J. P. Zehr</name>
</author>
<author><name sortKey="Mcreynolds, L A" uniqKey="Mcreynolds L">L. A. McReynolds</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhang, F" uniqKey="Zhang F">F. Zhang</name>
</author>
<author><name sortKey="Vicente, J" uniqKey="Vicente J">J. Vicente</name>
</author>
<author><name sortKey="Hill, R T" uniqKey="Hill R">R. T. Hill</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Front Microbiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Front Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">Front. Microbiol.</journal-id>
<journal-title-group><journal-title>Frontiers in Microbiology</journal-title>
</journal-title-group>
<issn pub-type="epub">1664-302X</issn>
<publisher><publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">25972851</article-id>
<article-id pub-id-type="pmc">4412061</article-id>
<article-id pub-id-type="doi">10.3389/fmicb.2015.00364</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Microbiology</subject>
<subj-group><subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Transcriptional activity of the giant barrel sponge, <italic>Xestospongia muta</italic>
Holobiont: molecular evidence for metabolic interchange</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Fiore</surname>
<given-names>Cara L.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="author-notes" rid="fn003"><sup>†</sup>
</xref>
<uri xlink:type="simple" xlink:href="http://community.frontiersin.org/people/u/197727"></uri>
</contrib>
<contrib contrib-type="author"><name><surname>Labrie</surname>
<given-names>Micheline</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="author-notes" rid="fn004"><sup>†</sup>
</xref>
<uri xlink:type="simple" xlink:href="http://community.frontiersin.org/people/u/213917"></uri>
</contrib>
<contrib contrib-type="author"><name><surname>Jarett</surname>
<given-names>Jessica K.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="author-notes" rid="fn005"><sup>†</sup>
</xref>
<uri xlink:type="simple" xlink:href="http://community.frontiersin.org/people/u/230632"></uri>
</contrib>
<contrib contrib-type="author"><name><surname>Lesser</surname>
<given-names>Michael P.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<xref ref-type="author-notes" rid="fn001"><sup>*</sup>
</xref>
<uri xlink:type="simple" xlink:href="http://community.frontiersin.org/people/u/155678"></uri>
</contrib>
</contrib-group>
<aff id="aff1"><sup>1</sup>
<institution>Department of Molecular, Cellular and Biomedical Sciences, University of New Hampshire</institution>
<country>Durham, NH, USA</country>
</aff>
<aff id="aff2"><sup>2</sup>
<institution>School of Marine Science and Ocean Engineering, University of New Hampshire</institution>
<country>Durham, NH, USA</country>
</aff>
<author-notes><fn fn-type="edited-by"><p>Edited by: Mike Taylor, The University of Auckland, New Zealand</p>
</fn>
<fn fn-type="edited-by"><p>Reviewed by: Jose V. Lopez, Nova Southeastern University, USA; Jeremy Brian Weisz, Linfield College, USA; Christian Robert Voolstra, King Abdullah University of Science and Technology, Saudi Arabia</p>
</fn>
<corresp id="fn001">*Correspondence: Michael P. Lesser, University of New Hampshire, Rudman Hall, 46 College Rd., 03824 Durham, NH, USA <email xlink:type="simple">mpl@unh.edu</email>
</corresp>
<fn fn-type="other" id="fn002"><p>This article was submitted to Microbial Symbioses, a section of the journal Frontiers in Microbiology</p>
</fn>
<fn fn-type="present-address" id="fn003"><p>†Present Address: Cara L. Fiore, Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, MA, USA;</p>
</fn>
<fn fn-type="present-address" id="fn004"><p>Micheline Labrie, School of Marine Science and Technology, University of Massachusetts at Dartmouth, Dartmouth, MA, USA;</p>
</fn>
<fn fn-type="present-address" id="fn005"><p>Jessica K. Jarett, United States Department of Energy Joint Genome Institute, Walnut Creek, CA, USA</p>
</fn>
</author-notes>
<pub-date pub-type="epub"><day>28</day>
<month>4</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="collection"><year>2015</year>
</pub-date>
<volume>6</volume>
<elocation-id>364</elocation-id>
<history><date date-type="received"><day>09</day>
<month>2</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>10</day>
<month>4</month>
<year>2015</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2015 Fiore, Labrie, Jarett and Lesser.</copyright-statement>
<copyright-year>2015</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p>
</license>
</permissions>
<abstract><p>Compared to our understanding of the taxonomic composition of the symbiotic microbes in marine sponges, the functional diversity of these symbionts is largely unknown. Furthermore, the application of genomic, transcriptomic, and proteomic techniques to functional questions on sponge host-symbiont interactions is in its infancy. In this study, we generated a transcriptome for the host and a metatranscriptome of its microbial symbionts for the giant barrel sponge, <italic>Xestospongia muta</italic>
, from the Caribbean. In combination with a gene-specific approach, our goals were to (1) characterize genetic evidence for nitrogen cycling in <italic>X. muta</italic>
, an important limiting nutrient on coral reefs (2) identify which prokaryotic symbiont lineages are metabolically active and, (3) characterize the metabolic potential of the prokaryotic community. <italic>Xestospongia muta</italic>
expresses genes from multiple nitrogen transformation pathways that when combined with the abundance of this sponge, and previous data on dissolved inorganic nitrogen fluxes, shows that this sponge is an important contributor to nitrogen cycling biogeochemistry on coral reefs. Additionally, we observed significant differences in gene expression of the archaeal <italic>amo</italic>
A gene, which is involved in ammonia oxidation, between coral reef locations consistent with differences in the fluxes of dissolved inorganic nitrogen previously reported. In regards to symbiont metabolic potential, the genes in the biosynthetic pathways of several amino acids were present in the prokaryotic metatranscriptome dataset but in the host-derived transcripts only the catabolic reactions for these amino acids were present. A similar pattern was observed for the B vitamins (riboflavin, biotin, thiamin, cobalamin). These results expand our understanding of biogeochemical cycling in sponges, and the metabolic interchange highlighted here advances the field of symbiont physiology by elucidating specific metabolic pathways where there is high potential for host-prokaryote interactions.</p>
</abstract>
<kwd-group><kwd>ammonia oxidation</kwd>
<kwd>denitrification</kwd>
<kwd>B vitamins</kwd>
<kwd>sponge symbionts</kwd>
<kwd>metatranscriptome</kwd>
</kwd-group>
<counts><fig-count count="7"></fig-count>
<table-count count="1"></table-count>
<equation-count count="0"></equation-count>
<ref-count count="100"></ref-count>
<page-count count="18"></page-count>
<word-count count="12250"></word-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Fiore, Cara L" sort="Fiore, Cara L" uniqKey="Fiore C" first="Cara L." last="Fiore">Cara L. Fiore</name>
</noRegion>
<name sortKey="Jarett, Jessica K" sort="Jarett, Jessica K" uniqKey="Jarett J" first="Jessica K." last="Jarett">Jessica K. Jarett</name>
<name sortKey="Labrie, Micheline" sort="Labrie, Micheline" uniqKey="Labrie M" first="Micheline" last="Labrie">Micheline Labrie</name>
<name sortKey="Lesser, Michael P" sort="Lesser, Michael P" uniqKey="Lesser M" first="Michael P." last="Lesser">Michael P. Lesser</name>
</country>
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</record>
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